UniProtKB - P54292 (RHLR_PSEAE)
HTH-type quorum-sensing regulator RhlR
rhlR
Functioni
Quorum-sensing regulator that controls the expression of multiple virulence factors in response to extracellular signaling molecules called autoinducers (PubMed:7494482, PubMed:8522523, PubMed:15576196).
Involved, among others, in the transcriptional regulation of genes that are responsible for rhamnolipid surfactant biosynthesis (PubMed:8144472, PubMed:7604006, PubMed:14526008).
Acts by binding to a specific sequence in the rhlAB regulatory region, both in the presence and in the absence of its autoinducer (PubMed:14526008).
In the former case it activates transcription of the promoter, whereas in the latter it acts as a transcriptional repressor (PubMed:14526008).
Also regulates the expression of the rmlBDAC operon, encoding dTDP-L-rhamnose biosynthetic enzymes, by binding to the rml box in the promoter region (PubMed:22262098).
In addition, is involved in the regulation of the production of elastase (lasB) and pyocyanine (PubMed:8144472, PubMed:7604006, PubMed:8522523).
7 PublicationsActivity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 198 – 217 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA-binding transcription activator activity Source: CollecTF
- DNA-binding transcription factor activity Source: PHI-base
- DNA-binding transcription repressor activity Source: CollecTF
- signaling receptor activity Source: PHI-base
- transcription cis-regulatory region binding Source: CollecTF
GO - Biological processi
- gene expression Source: CACAO
- positive regulation of lipid biosynthetic process Source: PseudoCAP
- positive regulation of proteolysis Source: PseudoCAP
- positive regulation of pyocyanine biosynthetic process Source: PHI-base
- positive regulation of secondary metabolite biosynthetic process Source: PseudoCAP
- positive regulation of transcription, DNA-templated Source: CollecTF
- quorum sensing Source: UniProtKB-KW
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Quorum sensing, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type quorum-sensing regulator RhlRCuratedAlternative name(s): Elastase modulator1 Publication Regulatory protein RhlR |
Gene namesi | Name:rhlR1 Publication Synonyms:lasM1 Publication, vsmR1 Publication Ordered Locus Names:PA3477 |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA3477 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- protein-DNA complex Source: CollecTF
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Miscellaneous databases
PHI-basei | PHI:6748 PHI:6996 PHI:7656 PHI:8547 PHI:8822 |
Chemistry databases
ChEMBLi | CHEMBL3112386 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000184186 | 1 – 241 | HTH-type quorum-sensing regulator RhlRAdd BLAST | 241 |
Proteomic databases
PaxDbi | P54292 |
Expressioni
Inductioni
Gene expression databases
CollecTFi | EXPREG_00000b10 |
Interactioni
Subunit structurei
Homodimer in the absence of any acyl-L-homoserine lactone (PubMed:12657054). The presence of the autoinducer C4-HSL has no significant effect on dimerization whereas N-(3-oxododecanoyl)-L-homoserine lactone (3O-C12-HSL), the LasR inducer, is able to dissociate the RhlR homodimers into monomers (PubMed:12657054).
1 PublicationProtein-protein interaction databases
STRINGi | 287.DR97_4444 |
Chemistry databases
BindingDBi | P54292 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 174 – 239 | HTH luxR-typePROSITE-ProRule annotationAdd BLAST | 66 |
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_072786_7_1_6 |
InParanoidi | P54292 |
OMAi | NHTVDWY |
PhylomeDBi | P54292 |
Family and domain databases
CDDi | cd06170, LuxR_C_like, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 3.30.450.80, 1 hit |
InterProi | View protein in InterPro IPR016032, Sig_transdc_resp-reg_C-effctor IPR005143, TF_LuxR_autoind-bd_dom IPR036693, TF_LuxR_autoind-bd_dom_sf IPR000792, Tscrpt_reg_LuxR_C IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF03472, Autoind_bind, 1 hit PF00196, GerE, 1 hit |
PRINTSi | PR00038, HTHLUXR |
SMARTi | View protein in SMART SM00421, HTH_LUXR, 1 hit |
SUPFAMi | SSF46894, SSF46894, 1 hit SSF75516, SSF75516, 1 hit |
PROSITEi | View protein in PROSITE PS00622, HTH_LUXR_1, 1 hit PS50043, HTH_LUXR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MRNDGGFLLW WDGLRSEMQP IHDSQGVFAV LEKEVRRLGF DYYAYGVRHT
60 70 80 90 100
IPFTRPKTEV HGTYPKAWLE RYQMQNYGAV DPAILNGLRS SEMVVWSDSL
110 120 130 140 150
FDQSRMLWNE ARDWGLCVGA TLPIRAPNNL LSVLSVARDQ QNISSFEREE
160 170 180 190 200
IRLRLRCMIE LLTQKLTDLE HPMLMSNPVC LSHREREILQ WTADGKSSGE
210 220 230 240
IAIILSISES TVNFHHKNIQ KKFDAPNKTL AAAYAAALGL I
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 12 | D → H in AAA89073 (PubMed:7494482).Curated | 1 | |
Sequence conflicti | 16 | S → C in AAA89073 (PubMed:7494482).Curated | 1 | |
Sequence conflicti | 99 | S → R in AAA89073 (PubMed:7494482).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40458 Genomic DNA Translation: AAC44036.1 L08962 Genomic DNA Translation: AAA25983.1 U15644 Genomic DNA Translation: AAA89073.1 AE004091 Genomic DNA Translation: AAG06865.1 L28170 Genomic DNA Translation: AAA62130.1 |
PIRi | B83212 C53652 S70174 |
RefSeqi | NP_252167.1, NC_002516.2 WP_003119559.1, NZ_QZGE01000039.1 |
Genome annotation databases
EnsemblBacteriai | AAG06865; AAG06865; PA3477 |
GeneIDi | 878968 |
KEGGi | pae:PA3477 |
PATRICi | fig|208964.12.peg.3640 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40458 Genomic DNA Translation: AAC44036.1 L08962 Genomic DNA Translation: AAA25983.1 U15644 Genomic DNA Translation: AAA89073.1 AE004091 Genomic DNA Translation: AAG06865.1 L28170 Genomic DNA Translation: AAA62130.1 |
PIRi | B83212 C53652 S70174 |
RefSeqi | NP_252167.1, NC_002516.2 WP_003119559.1, NZ_QZGE01000039.1 |
3D structure databases
AlphaFoldDBi | P54292 |
SMRi | P54292 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 287.DR97_4444 |
Chemistry databases
BindingDBi | P54292 |
ChEMBLi | CHEMBL3112386 |
Proteomic databases
PaxDbi | P54292 |
Genome annotation databases
EnsemblBacteriai | AAG06865; AAG06865; PA3477 |
GeneIDi | 878968 |
KEGGi | pae:PA3477 |
PATRICi | fig|208964.12.peg.3640 |
Organism-specific databases
PseudoCAPi | PA3477 |
Phylogenomic databases
HOGENOMi | CLU_072786_7_1_6 |
InParanoidi | P54292 |
OMAi | NHTVDWY |
PhylomeDBi | P54292 |
Miscellaneous databases
PHI-basei | PHI:6748 PHI:6996 PHI:7656 PHI:8547 PHI:8822 |
Gene expression databases
CollecTFi | EXPREG_00000b10 |
Family and domain databases
CDDi | cd06170, LuxR_C_like, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 3.30.450.80, 1 hit |
InterProi | View protein in InterPro IPR016032, Sig_transdc_resp-reg_C-effctor IPR005143, TF_LuxR_autoind-bd_dom IPR036693, TF_LuxR_autoind-bd_dom_sf IPR000792, Tscrpt_reg_LuxR_C IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF03472, Autoind_bind, 1 hit PF00196, GerE, 1 hit |
PRINTSi | PR00038, HTHLUXR |
SMARTi | View protein in SMART SM00421, HTH_LUXR, 1 hit |
SUPFAMi | SSF46894, SSF46894, 1 hit SSF75516, SSF75516, 1 hit |
PROSITEi | View protein in PROSITE PS00622, HTH_LUXR_1, 1 hit PS50043, HTH_LUXR_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RHLR_PSEAE | |
Accessioni | P54292Primary (citable) accession number: P54292 Secondary accession number(s): Q9HYD2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families