UniProtKB - P54287 (CACB3_RAT)
Voltage-dependent L-type calcium channel subunit beta-3
Cacnb3
Functioni
Regulatory subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:7679112, PubMed:10666413, PubMed:24751537, PubMed:29742403, PubMed:15170217).
Increases CACNA1B peak calcium current and shifts the voltage dependencies of channel activation and inactivation (By similarity).
Increases CACNA1C peak calcium current and shifts the voltage dependencies of channel activation and inactivation (PubMed:7679112, PubMed:10666413, PubMed:24751537, PubMed:29742403, PubMed:15170217).
By similarity5 PublicationsGO - Molecular functioni
- calcium channel regulator activity Source: UniProtKB
- protein kinase binding Source: RGD
- voltage-gated calcium channel activity Source: RGD
GO - Biological processi
- calcium ion transmembrane transport Source: BHF-UCL
- calcium ion transmembrane transport via high voltage-gated calcium channel Source: UniProtKB
- calcium ion transport Source: RGD
- calcium ion transport into cytosol Source: BHF-UCL
- cellular response to oxygen-glucose deprivation Source: RGD
- chemical synaptic transmission Source: GO_Central
- detection of mechanical stimulus involved in sensory perception of pain Source: RGD
- negative regulation of detection of mechanical stimulus involved in sensory perception of touch Source: RGD
- negative regulation of voltage-gated calcium channel activity Source: RGD
- neuromuscular junction development Source: GO_Central
- positive regulation of excitatory postsynaptic potential Source: RGD
- positive regulation of high voltage-gated calcium channel activity Source: UniProtKB
- positive regulation of protein targeting to membrane Source: RGD
- positive regulation of voltage-gated calcium channel activity Source: RGD
- protein localization to plasma membrane Source: BHF-UCL
- regulation of membrane hyperpolarization Source: RGD
- regulation of membrane repolarization during action potential Source: BHF-UCL
- regulation of voltage-gated calcium channel activity Source: GO_Central
- T cell receptor signaling pathway Source: RGD
Keywordsi
Molecular function | Calcium channel, Ion channel, Voltage-gated channel |
Biological process | Calcium transport, Ion transport, Transport |
Ligand | Calcium |
Enzyme and pathway databases
Reactomei | R-RNO-112308, Presynaptic depolarization and calcium channel opening R-RNO-422356, Regulation of insulin secretion |
Names & Taxonomyi
Protein namesi | Recommended name: Voltage-dependent L-type calcium channel subunit beta-3Short name: CAB3 Alternative name(s): Calcium channel voltage-dependent subunit beta 3 |
Gene namesi | Name:Cacnb3 Synonyms:Cacnlb3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2248, Cacnb3 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Plasma Membrane
- apical plasma membrane Source: RGD
- L-type voltage-gated calcium channel complex Source: UniProtKB
- voltage-gated calcium channel complex Source: RGD
Other locations
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144059 | 1 – 484 | Voltage-dependent L-type calcium channel subunit beta-3Add BLAST | 484 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 152 | PhosphoserineCombined sources | 1 | |
Modified residuei | 393 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P54287 |
PRIDEi | P54287 |
PTM databases
iPTMneti | P54287 |
PhosphoSitePlusi | P54287 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000054274, Expressed in brain and 21 other tissues |
Genevisiblei | P54287, RN |
Interactioni
Subunit structurei
Component of a calcium channel complex consisting of a pore-forming alpha subunit (CACNA1C) and the ancillary subunits CACNB3 and CACNA2D1 (Probable). The channel complex contains alpha, beta, gamma and delta subunits in a 1:1:1:1 ratio (Probable).
Interacts with CACNA2D4.
Interacts with FASLG (By similarity).
Interacts with CBARP; prevents the interaction of CACNB3 with the alpha subunit CACNA1C thereby negatively regulating the activity of the corresponding calcium channel (PubMed:24751537).
By similarity4 Publications1 PublicationBinary interactionsi
P54287
With | #Exp. | IntAct |
---|---|---|
GEM [P55040] from Homo sapiens. | 2 | EBI-8028596,EBI-744104 |
GO - Molecular functioni
- protein kinase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 247336, 3 interactors |
DIPi | DIP-59370N |
IntActi | P54287, 3 interactors |
MINTi | P54287 |
STRINGi | 10116.ENSRNOP00000017490 |
Chemistry databases
BindingDBi | P54287 |
Structurei
Secondary structure
3D structure databases
SMRi | P54287 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P54287 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 59 – 128 | SH3PROSITE-ProRule annotationAdd BLAST | 70 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 52 | DisorderedSequence analysisAdd BLAST | 52 | |
Regioni | 129 – 170 | DisorderedSequence analysisAdd BLAST | 42 | |
Regioni | 195 – 345 | Mediates interaction with the alpha subunit1 PublicationAdd BLAST | 151 | |
Regioni | 388 – 484 | DisorderedSequence analysisAdd BLAST | 97 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 29 – 52 | Basic and acidic residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 136 – 159 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 389 – 404 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 405 – 420 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 458 – 484 | Basic and acidic residuesSequence analysisAdd BLAST | 27 |
Sequence similaritiesi
Keywords - Domaini
SH3 domainPhylogenomic databases
eggNOGi | KOG3812, Eukaryota |
GeneTreei | ENSGT00950000182837 |
HOGENOMi | CLU_021995_0_1_1 |
InParanoidi | P54287 |
OMAi | QEVELIM |
OrthoDBi | 926074at2759 |
PhylomeDBi | P54287 |
TreeFami | TF316195 |
Family and domain databases
CDDi | cd12042, SH3_CACNB3, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR035760, CACNB3_SH3 IPR008145, GK/Ca_channel_bsu IPR027417, P-loop_NTPase IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR008079, VDCC_L_b3su IPR000584, VDCC_L_bsu |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF12052, VGCC_beta4Aa_N, 1 hit |
PRINTSi | PR01626, LCACHANNELB PR01696, LCACHANNELB3 |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MYDDSYVPGF EDSEAGSADS YTSRPSLDSD VSLEEDRESA RREVESQAQQ
60 70 80 90 100
QLERAKHKPV AFAVRTNVSY CGVLDEECPV QGSGVNFEAK DFLHIKEKYS
110 120 130 140 150
NDWWIGRLVK EGGDIAFIPS PQRLESIRLK QEQKARRSGN PSSLSDIGNR
160 170 180 190 200
RSPPPSLAKQ KQKQAEHVPP YDVVPSMRPV VLVGPSLKGY EVTDMMQKAL
210 220 230 240 250
FDFLKHRFDG RISITRVTAD LSLAKRSVLN NPGKRTIIER SSARSSIAEV
260 270 280 290 300
QSEIERIFEL AKSLQLVVLD ADTINHPAQL AKTSLAPIIV FVKVSSPKVL
310 320 330 340 350
QRLIRSRGKS QMKHLTVQMM AYDKLVQCPP ESFDVILDEN QLDDACEHLA
360 370 380 390 400
EYLEVYWRAT HHPAPGPGML GPPSAIPGLQ NQQLLGERGE EHSPLERDSL
410 420 430 440 450
MPSDEASESS RQAWTGSSQR SSRHLEEDYA DAYQDLYQPH RQHTSGLPSA
460 470 480
NGHDPQDRLL AQDSEHDHND RNWQRNRPWP KDSY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88751 mRNA Translation: AAA18486.1 |
PIRi | A46608 |
RefSeqi | NP_036960.1, NM_012828.2 |
Genome annotation databases
Ensembli | ENSRNOT00000081206; ENSRNOP00000073026; ENSRNOG00000054274 |
GeneIDi | 25297 |
KEGGi | rno:25297 |
UCSCi | RGD:2248, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88751 mRNA Translation: AAA18486.1 |
PIRi | A46608 |
RefSeqi | NP_036960.1, NM_012828.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1VYT | X-ray | 2.60 | A/B | 16-366 | [»] | |
1VYU | X-ray | 2.30 | A/B | 16-366 | [»] | |
SMRi | P54287 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 247336, 3 interactors |
DIPi | DIP-59370N |
IntActi | P54287, 3 interactors |
MINTi | P54287 |
STRINGi | 10116.ENSRNOP00000017490 |
Chemistry databases
BindingDBi | P54287 |
ChEMBLi | CHEMBL3137269 CHEMBL3137270 CHEMBL4296086 |
PTM databases
iPTMneti | P54287 |
PhosphoSitePlusi | P54287 |
Proteomic databases
PaxDbi | P54287 |
PRIDEi | P54287 |
Genome annotation databases
Ensembli | ENSRNOT00000081206; ENSRNOP00000073026; ENSRNOG00000054274 |
GeneIDi | 25297 |
KEGGi | rno:25297 |
UCSCi | RGD:2248, rat |
Organism-specific databases
CTDi | 784 |
RGDi | 2248, Cacnb3 |
Phylogenomic databases
eggNOGi | KOG3812, Eukaryota |
GeneTreei | ENSGT00950000182837 |
HOGENOMi | CLU_021995_0_1_1 |
InParanoidi | P54287 |
OMAi | QEVELIM |
OrthoDBi | 926074at2759 |
PhylomeDBi | P54287 |
TreeFami | TF316195 |
Enzyme and pathway databases
Reactomei | R-RNO-112308, Presynaptic depolarization and calcium channel opening R-RNO-422356, Regulation of insulin secretion |
Miscellaneous databases
EvolutionaryTracei | P54287 |
PROi | PR:P54287 |
Gene expression databases
Bgeei | ENSRNOG00000054274, Expressed in brain and 21 other tissues |
Genevisiblei | P54287, RN |
Family and domain databases
CDDi | cd12042, SH3_CACNB3, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR035760, CACNB3_SH3 IPR008145, GK/Ca_channel_bsu IPR027417, P-loop_NTPase IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR008079, VDCC_L_b3su IPR000584, VDCC_L_bsu |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF12052, VGCC_beta4Aa_N, 1 hit |
PRINTSi | PR01626, LCACHANNELB PR01696, LCACHANNELB3 |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CACB3_RAT | |
Accessioni | P54287Primary (citable) accession number: P54287 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | September 29, 2021 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families