UniProtKB - P54284 (CACB3_HUMAN)
Voltage-dependent L-type calcium channel subunit beta-3
CACNB3
Functioni
Regulatory subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:8119293).
Increases CACNA1B peak calcium current and shifts the voltage dependencies of channel activation and inactivation (By similarity).
Increases CACNA1C peak calcium current and shifts the voltage dependencies of channel activation and inactivation (By similarity).
By similarity1 PublicationGO - Molecular functioni
- calcium channel regulator activity Source: UniProtKB
- high voltage-gated calcium channel activity Source: GO_Central
- voltage-gated calcium channel activity Source: ProtInc
GO - Biological processi
- calcium ion transmembrane transport via high voltage-gated calcium channel Source: UniProtKB
- calcium ion transport Source: UniProtKB
- calcium ion transport into cytosol Source: BHF-UCL
- chemical synaptic transmission Source: GO_Central
- neuromuscular junction development Source: GO_Central
- positive regulation of high voltage-gated calcium channel activity Source: UniProtKB
- protein localization to plasma membrane Source: BHF-UCL
- regulation of membrane repolarization during action potential Source: BHF-UCL
- regulation of voltage-gated calcium channel activity Source: GO_Central
- T cell receptor signaling pathway Source: Ensembl
Keywordsi
Molecular function | Calcium channel, Ion channel, Voltage-gated channel |
Biological process | Calcium transport, Ion transport, Transport |
Ligand | Calcium |
Enzyme and pathway databases
PathwayCommonsi | P54284 |
Reactomei | R-HSA-112308, Presynaptic depolarization and calcium channel opening R-HSA-400042, Adrenaline,noradrenaline inhibits insulin secretion R-HSA-419037, NCAM1 interactions R-HSA-422356, Regulation of insulin secretion |
SignaLinki | P54284 |
Protein family/group databases
TCDBi | 8.A.22.1.3, the ca(2+) channel auxiliary subunit Beta types 1-4 (cca-Beta) family |
Names & Taxonomyi
Protein namesi | Recommended name: Voltage-dependent L-type calcium channel subunit beta-3Short name: CAB3 Alternative name(s): Calcium channel voltage-dependent subunit beta 3 |
Gene namesi | Name:CACNB3 Synonyms:CACNLB31 Publication |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1403, CACNB3 |
MIMi | 601958, gene |
neXtProti | NX_P54284 |
VEuPathDBi | HostDB:ENSG00000167535 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: Reactome
Plasma Membrane
- L-type voltage-gated calcium channel complex Source: UniProtKB
- voltage-gated calcium channel complex Source: UniProtKB
Other locations
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 784 |
OpenTargetsi | ENSG00000167535 |
PharmGKBi | PA89 |
Miscellaneous databases
Pharosi | P54284, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3430901 |
DrugBanki | DB01118, Amiodarone DB09231, Benidipine DB13746, Bioallethrin DB11148, Butamben DB11093, Calcium citrate DB11348, Calcium Phosphate DB14481, Calcium phosphate dihydrate DB09232, Cilnidipine DB04855, Dronedarone DB00228, Enflurane DB00153, Ergocalciferol DB13961, Fish oil DB09236, Lacidipine DB00825, Levomenthol DB00653, Magnesium sulfate DB09238, Manidipine DB01388, Mibefradil DB01110, Miconazole DB00393, Nimodipine DB00252, Phenytoin DB00243, Ranolazine DB00421, Spironolactone DB00273, Topiramate DB09089, Trimebutine |
Genetic variation databases
BioMutai | CACNB3 |
DMDMi | 1705683 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144056 | 1 – 484 | Voltage-dependent L-type calcium channel subunit beta-3Add BLAST | 484 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 152 | PhosphoserineBy similarity | 1 | |
Modified residuei | 393 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P54284 |
MassIVEi | P54284 |
MaxQBi | P54284 |
PaxDbi | P54284 |
PeptideAtlasi | P54284 |
PRIDEi | P54284 |
ProteomicsDBi | 25153 26032 28606 56673 [P54284-1] 56674 [P54284-2] |
PTM databases
iPTMneti | P54284 |
PhosphoSitePlusi | P54284 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000167535, Expressed in right frontal lobe and 210 other tissues |
ExpressionAtlasi | P54284, baseline and differential |
Genevisiblei | P54284, HS |
Organism-specific databases
HPAi | ENSG00000167535, Tissue enhanced (brain) |
Interactioni
Subunit structurei
Component of a calcium channel complex consisting of a pore-forming alpha subunit (CACNA1C) and the ancillary subunits CACNB3 and CACNA2D1 (PubMed:29742403). The channel complex contains alpha, beta, gamma and delta subunits in a 1:1:1:1 ratio (Probable).
Interacts with CACNA2D4 (PubMed:12181424).
Interacts with FASLG (PubMed:19807924).
Interacts with CBARP; prevents the interaction of CACNB3 with the alpha subunit CACNA1C thereby negatively regulating the activity of the corresponding calcium channel (By similarity).
By similarityCurated3 PublicationsBinary interactionsi
P54284
Protein-protein interaction databases
BioGRIDi | 107238, 27 interactors |
DIPi | DIP-39138N |
IntActi | P54284, 19 interactors |
STRINGi | 9606.ENSP00000301050 |
Chemistry databases
BindingDBi | P54284 |
Miscellaneous databases
RNActi | P54284, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 59 – 128 | SH3PROSITE-ProRule annotationAdd BLAST | 70 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 52 | DisorderedSequence analysisAdd BLAST | 52 | |
Regioni | 129 – 170 | DisorderedSequence analysisAdd BLAST | 42 | |
Regioni | 195 – 345 | Mediates interaction with the alpha subunitBy similarityAdd BLAST | 151 | |
Regioni | 387 – 484 | DisorderedSequence analysisAdd BLAST | 98 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 29 – 52 | Basic and acidic residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 136 – 159 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 389 – 404 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 405 – 420 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 458 – 484 | Basic and acidic residuesSequence analysisAdd BLAST | 27 |
Sequence similaritiesi
Keywords - Domaini
SH3 domainPhylogenomic databases
eggNOGi | KOG3812, Eukaryota |
GeneTreei | ENSGT00950000182837 |
HOGENOMi | CLU_021995_0_1_1 |
InParanoidi | P54284 |
OMAi | QEVELIM |
OrthoDBi | 926074at2759 |
PhylomeDBi | P54284 |
TreeFami | TF316195 |
Family and domain databases
CDDi | cd12042, SH3_CACNB3, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR035760, CACNB3_SH3 IPR008145, GK/Ca_channel_bsu IPR027417, P-loop_NTPase IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR008079, VDCC_L_b3su IPR000584, VDCC_L_bsu |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF12052, VGCC_beta4Aa_N, 1 hit |
PRINTSi | PR01626, LCACHANNELB PR01696, LCACHANNELB3 |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MYDDSYVPGF EDSEAGSADS YTSRPSLDSD VSLEEDRESA RREVESQAQQ
60 70 80 90 100
QLERAKHKPV AFAVRTNVSY CGVLDEECPV QGSGVNFEAK DFLHIKEKYS
110 120 130 140 150
NDWWIGRLVK EGGDIAFIPS PQRLESIRLK QEQKARRSGN PSSLSDIGNR
160 170 180 190 200
RSPPPSLAKQ KQKQAEHVPP YDVVPSMRPV VLVGPSLKGY EVTDMMQKAL
210 220 230 240 250
FDFLKHRFDG RISITRVTAD LSLAKRSVLN NPGKRTIIER SSARSSIAEV
260 270 280 290 300
QSEIERIFEL AKSLQLVVLD ADTINHPAQL AKTSLAPIIV FVKVSSPKVL
310 320 330 340 350
QRLIRSRGKS QMKHLTVQMM AYDKLVQCPP ESFDVILDEN QLEDACEHLA
360 370 380 390 400
EYLEVYWRAT HHPAPGPGLL GPPSAIPGLQ NQQLLGERGE EHSPLERDSL
410 420 430 440 450
MPSDEASESS RQAWTGSSQR SSRHLEEDYA DAYQDLYQPH RQHTSGLPSA
460 470 480
NGHDPQDRLL AQDSEHNHSD RNWQRNRPWP KDSY
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VNV8 | F8VNV8_HUMAN | Calcium channel voltage-dependent s... | CACNB3 | 457 | Annotation score: | ||
F8VV14 | F8VV14_HUMAN | Voltage-dependent L-type calcium ch... | CACNB3 | 151 | Annotation score: | ||
F8VWK1 | F8VWK1_HUMAN | Voltage-dependent L-type calcium ch... | CACNB3 | 144 | Annotation score: | ||
F8W0F8 | F8W0F8_HUMAN | Voltage-dependent L-type calcium ch... | CACNB3 | 105 | Annotation score: | ||
H0YHY2 | H0YHY2_HUMAN | Voltage-dependent L-type calcium ch... | CACNB3 | 76 | Annotation score: | ||
F8VUW8 | F8VUW8_HUMAN | Voltage-dependent L-type calcium ch... | CACNB3 | 122 | Annotation score: | ||
F8VU10 | F8VU10_HUMAN | Voltage-dependent L-type calcium ch... | CACNB3 | 55 | Annotation score: | ||
F8W1N3 | F8W1N3_HUMAN | Voltage-dependent L-type calcium ch... | CACNB3 | 37 | Annotation score: | ||
H0YHK1 | H0YHK1_HUMAN | Voltage-dependent L-type calcium ch... | CACNB3 | 84 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 7 | V → L in AAA19799 (PubMed:8119293).Curated | 1 | |
Sequence conflicti | 32 | Missing in AAA19799 (PubMed:8119293).Curated | 1 | |
Sequence conflicti | 60 | V → E in AAA19799 (PubMed:8119293).Curated | 1 | |
Sequence conflicti | 101 | N → D in BAH14694 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 162 | Q → R in BAH14209 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 196 | M → T in BAH12637 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 245 | S → P in BAH12637 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 320 | Missing in AAA19799 (PubMed:8119293).Curated | 1 | |
Sequence conflicti | 348 | H → L in AAA19799 (PubMed:8119293).Curated | 1 | |
Sequence conflicti | 421 | S → T in AAA19799 (PubMed:8119293).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_024384 | 423 | R → H. Corresponds to variant dbSNP:rs2229954Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046708 | 1 – 15 | MYDDS…EDSEA → ME in isoform 3. 1 PublicationAdd BLAST | 15 | |
Alternative sequenceiVSP_046709 | 1 – 15 | MYDDS…EDSEA → MSFSDSSATFLLNE in isoform 4. 1 PublicationAdd BLAST | 15 | |
Alternative sequenceiVSP_046710 | 57 – 97 | Missing in isoform 5. CuratedAdd BLAST | 41 | |
Alternative sequenceiVSP_000634 | 440 – 484 | HRQHT…PKDSY → QCQLLSLL in isoform 2. 1 PublicationAdd BLAST | 45 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76555 mRNA Translation: CAA54055.1 X76556 mRNA Translation: CAA54056.1 D43704 Genomic DNA Translation: BAA07803.1 U07139 mRNA Translation: AAA95958.1 L27584 mRNA Translation: AAA19799.1 AK289709 mRNA Translation: BAF82398.1 AK297639 mRNA Translation: BAH12637.1 AK304537 mRNA Translation: BAH14209.1 AK316323 mRNA Translation: BAH14694.1 AC117498 Genomic DNA No translation available. CH471111 Genomic DNA Translation: EAW58008.1 BC041811 mRNA Translation: AAH41811.1 |
CCDSi | CCDS55821.1 [P54284-3] CCDS55822.1 [P54284-4] CCDS55823.1 [P54284-5] CCDS8769.1 [P54284-1] |
PIRi | S39315 S39316 S41211 |
RefSeqi | NP_000716.2, NM_000725.3 [P54284-1] NP_001193844.1, NM_001206915.1 [P54284-5] NP_001193845.1, NM_001206916.1 [P54284-4] NP_001193846.1, NM_001206917.1 [P54284-3] |
Genome annotation databases
Ensembli | ENST00000301050; ENSP00000301050; ENSG00000167535 ENST00000536187; ENSP00000444160; ENSG00000167535 [P54284-4] ENST00000540990; ENSP00000445495; ENSG00000167535 [P54284-3] ENST00000547230; ENSP00000448304; ENSG00000167535 [P54284-5] |
GeneIDi | 784 |
KEGGi | hsa:784 |
MANE-Selecti | ENST00000301050.7; ENSP00000301050.2; NM_000725.4; NP_000716.2 |
UCSCi | uc001rsl.3, human [P54284-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76555 mRNA Translation: CAA54055.1 X76556 mRNA Translation: CAA54056.1 D43704 Genomic DNA Translation: BAA07803.1 U07139 mRNA Translation: AAA95958.1 L27584 mRNA Translation: AAA19799.1 AK289709 mRNA Translation: BAF82398.1 AK297639 mRNA Translation: BAH12637.1 AK304537 mRNA Translation: BAH14209.1 AK316323 mRNA Translation: BAH14694.1 AC117498 Genomic DNA No translation available. CH471111 Genomic DNA Translation: EAW58008.1 BC041811 mRNA Translation: AAH41811.1 |
CCDSi | CCDS55821.1 [P54284-3] CCDS55822.1 [P54284-4] CCDS55823.1 [P54284-5] CCDS8769.1 [P54284-1] |
PIRi | S39315 S39316 S41211 |
RefSeqi | NP_000716.2, NM_000725.3 [P54284-1] NP_001193844.1, NM_001206915.1 [P54284-5] NP_001193845.1, NM_001206916.1 [P54284-4] NP_001193846.1, NM_001206917.1 [P54284-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7MIX | electron microscopy | 3.00 | C | 1-484 | [»] | |
7MIY | electron microscopy | 3.10 | C | 1-484 | [»] | |
SMRi | P54284 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107238, 27 interactors |
DIPi | DIP-39138N |
IntActi | P54284, 19 interactors |
STRINGi | 9606.ENSP00000301050 |
Chemistry databases
BindingDBi | P54284 |
ChEMBLi | CHEMBL3430901 |
DrugBanki | DB01118, Amiodarone DB09231, Benidipine DB13746, Bioallethrin DB11148, Butamben DB11093, Calcium citrate DB11348, Calcium Phosphate DB14481, Calcium phosphate dihydrate DB09232, Cilnidipine DB04855, Dronedarone DB00228, Enflurane DB00153, Ergocalciferol DB13961, Fish oil DB09236, Lacidipine DB00825, Levomenthol DB00653, Magnesium sulfate DB09238, Manidipine DB01388, Mibefradil DB01110, Miconazole DB00393, Nimodipine DB00252, Phenytoin DB00243, Ranolazine DB00421, Spironolactone DB00273, Topiramate DB09089, Trimebutine |
Protein family/group databases
TCDBi | 8.A.22.1.3, the ca(2+) channel auxiliary subunit Beta types 1-4 (cca-Beta) family |
PTM databases
iPTMneti | P54284 |
PhosphoSitePlusi | P54284 |
Genetic variation databases
BioMutai | CACNB3 |
DMDMi | 1705683 |
Proteomic databases
jPOSTi | P54284 |
MassIVEi | P54284 |
MaxQBi | P54284 |
PaxDbi | P54284 |
PeptideAtlasi | P54284 |
PRIDEi | P54284 |
ProteomicsDBi | 25153 26032 28606 56673 [P54284-1] 56674 [P54284-2] |
Protocols and materials databases
Antibodypediai | 13690, 210 antibodies from 30 providers |
DNASUi | 784 |
Genome annotation databases
Ensembli | ENST00000301050; ENSP00000301050; ENSG00000167535 ENST00000536187; ENSP00000444160; ENSG00000167535 [P54284-4] ENST00000540990; ENSP00000445495; ENSG00000167535 [P54284-3] ENST00000547230; ENSP00000448304; ENSG00000167535 [P54284-5] |
GeneIDi | 784 |
KEGGi | hsa:784 |
MANE-Selecti | ENST00000301050.7; ENSP00000301050.2; NM_000725.4; NP_000716.2 |
UCSCi | uc001rsl.3, human [P54284-1] |
Organism-specific databases
CTDi | 784 |
DisGeNETi | 784 |
GeneCardsi | CACNB3 |
HGNCi | HGNC:1403, CACNB3 |
HPAi | ENSG00000167535, Tissue enhanced (brain) |
MIMi | 601958, gene |
neXtProti | NX_P54284 |
OpenTargetsi | ENSG00000167535 |
PharmGKBi | PA89 |
VEuPathDBi | HostDB:ENSG00000167535 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3812, Eukaryota |
GeneTreei | ENSGT00950000182837 |
HOGENOMi | CLU_021995_0_1_1 |
InParanoidi | P54284 |
OMAi | QEVELIM |
OrthoDBi | 926074at2759 |
PhylomeDBi | P54284 |
TreeFami | TF316195 |
Enzyme and pathway databases
PathwayCommonsi | P54284 |
Reactomei | R-HSA-112308, Presynaptic depolarization and calcium channel opening R-HSA-400042, Adrenaline,noradrenaline inhibits insulin secretion R-HSA-419037, NCAM1 interactions R-HSA-422356, Regulation of insulin secretion |
SignaLinki | P54284 |
Miscellaneous databases
BioGRID-ORCSi | 784, 204 hits in 1039 CRISPR screens |
ChiTaRSi | CACNB3, human |
GeneWikii | CACNB3 |
GenomeRNAii | 784 |
Pharosi | P54284, Tbio |
PROi | PR:P54284 |
RNActi | P54284, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000167535, Expressed in right frontal lobe and 210 other tissues |
ExpressionAtlasi | P54284, baseline and differential |
Genevisiblei | P54284, HS |
Family and domain databases
CDDi | cd12042, SH3_CACNB3, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR035760, CACNB3_SH3 IPR008145, GK/Ca_channel_bsu IPR027417, P-loop_NTPase IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR008079, VDCC_L_b3su IPR000584, VDCC_L_bsu |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF12052, VGCC_beta4Aa_N, 1 hit |
PRINTSi | PR01626, LCACHANNELB PR01696, LCACHANNELB3 |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CACB3_HUMAN | |
Accessioni | P54284Primary (citable) accession number: P54284 Secondary accession number(s): A8K0Z4 Q13913 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 199 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families