UniProtKB - P54219 (VMAT1_HUMAN)
Protein
Chromaffin granule amine transporter
Gene
SLC18A1
Organism
Homo sapiens (Human)
Status
Functioni
Involved in the transport of biogenic monoamines, such as serotonin, from the cytoplasm into the secretory vesicles of neuroendocrine and endocrine cells.2 Publications
Activity regulationi
Strongly inhibited by reserpine. Also inhibited to a lesser extent by ketanserin and fenfluramine. Not significantly inhibited by tetrabenazine.1 Publication
GO - Molecular functioni
- monoamine transmembrane transporter activity Source: GO_Central
- serotonin:sodium symporter activity Source: GO_Central
- transmembrane transporter activity Source: GO_Central
- xenobiotic transmembrane transporter activity Source: InterPro
GO - Biological processi
- aminergic neurotransmitter loading into synaptic vesicle Source: GO_Central
- monoamine transport Source: UniProtKB
- neurotransmitter transport Source: GO_Central
Keywordsi
Biological process | Neurotransmitter transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | P54219 |
Reactomei | R-HSA-442660, Na+/Cl- dependent neurotransmitter transporters |
SIGNORi | P54219 |
Protein family/group databases
TCDBi | 2.A.1.2.12, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Chromaffin granule amine transporterAlternative name(s): Solute carrier family 18 member 1 Vesicular amine transporter 1 Short name: VAT1 |
Gene namesi | Name:SLC18A1 Synonyms:VAT1, VMAT1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000036565.14 |
HGNCi | HGNC:10934, SLC18A1 |
MIMi | 193002, gene |
neXtProti | NX_P54219 |
Subcellular locationi
Other locations
- Cytoplasmic vesicle membrane 2 Publications; Multi-pass membrane protein 1 Publication
- synaptic vesicle membrane By similarity; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- clathrin-sculpted monoamine transport vesicle membrane Source: Reactome
- integral component of membrane Source: UniProtKB-KW
- synaptic vesicle membrane Source: GO_Central
- terminal bouton Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 21 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 22 – 42 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 43 – 138 | Lumenal, vesicleSequence analysisAdd BLAST | 96 | |
Transmembranei | 139 – 158 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 159 – 167 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 168 – 188 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 189 – 197 | Lumenal, vesicleSequence analysis | 9 | |
Transmembranei | 198 – 218 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 219 – 227 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 228 – 250 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 251 – 256 | Lumenal, vesicleSequence analysis | 6 | |
Transmembranei | 257 – 279 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 280 – 299 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 300 – 319 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 320 – 335 | Lumenal, vesicleSequence analysisAdd BLAST | 16 | |
Transmembranei | 336 – 360 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 361 – 365 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 366 – 386 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 387 – 397 | Lumenal, vesicleSequence analysisAdd BLAST | 11 | |
Transmembranei | 398 – 418 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 419 – 422 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 423 – 443 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 444 – 448 | Lumenal, vesicleSequence analysis | 5 | |
Transmembranei | 449 – 470 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 471 – 525 | CytoplasmicSequence analysisAdd BLAST | 55 |
Keywords - Cellular componenti
Cell junction, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, SynapsePathology & Biotechi
Organism-specific databases
DisGeNETi | 6570 |
OpenTargetsi | ENSG00000036565 |
PharmGKBi | PA324 |
Miscellaneous databases
Pharosi | P54219, Tchem |
Chemistry databases
ChEMBLi | CHEMBL1838 |
DrugBanki | DB01363, Ephedra sinica root DB01577, Metamfetamine DB01442, MMDA DB00368, Norepinephrine DB00206, Reserpine |
DrugCentrali | P54219 |
GuidetoPHARMACOLOGYi | 1011 |
Polymorphism and mutation databases
BioMutai | SLC18A1 |
DMDMi | 1722741 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127510 | 1 – 525 | Chromaffin granule amine transporterAdd BLAST | 525 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 58 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 87 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 104 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
MassIVEi | P54219 |
PaxDbi | P54219 |
PeptideAtlasi | P54219 |
PRIDEi | P54219 |
ProteomicsDBi | 19679 56654 [P54219-1] |
PTM databases
GlyGeni | P54219, 3 sites |
iPTMneti | P54219 |
PhosphoSitePlusi | P54219 |
Expressioni
Tissue specificityi
Highly expressed in chromaffin cells of the adrenal medulla (at protein level). Detected in peripheral sympathetic ganglia (at protein level). Found in some paracrine cells in stomach and duodenum (at protein level).1 Publication
Gene expression databases
Bgeei | ENSG00000036565, Expressed in adrenal gland and 74 other tissues |
ExpressionAtlasi | P54219, baseline and differential |
Genevisiblei | P54219, HS |
Organism-specific databases
HPAi | ENSG00000036565, Tissue enhanced (adrenal gland, intestine) |
Interactioni
Binary interactionsi
Isoform 3 [P54219-3]
Protein-protein interaction databases
BioGRIDi | 112458, 127 interactors |
IntActi | P54219, 104 interactors |
STRINGi | 9606.ENSP00000387549 |
Chemistry databases
BindingDBi | P54219 |
Miscellaneous databases
RNActi | P54219, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3764, Eukaryota |
GeneTreei | ENSGT00940000159352 |
HOGENOMi | CLU_001265_10_9_1 |
InParanoidi | P54219 |
OMAi | RVGYHIP |
PhylomeDBi | P54219 |
TreeFami | TF313494 |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR004734, Multidrug-R |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00880, 2_A_01_02, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P54219-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLRTILDAPQ RLLKEGRASR QLVLVVVFVA LLLDNMLFTV VVPIVPTFLY
60 70 80 90 100
DMEFKEVNSS LHLGHAGSSP HALASPAFST IFSFFNNNTV AVEESVPSGI
110 120 130 140 150
AWMNDTASTI PPPATEAISA HKNNCLQGTG FLEEEITRVG VLFASKAVMQ
160 170 180 190 200
LLVNPFVGPL TNRIGYHIPM FAGFVIMFLS TVMFAFSGTY TLLFVARTLQ
210 220 230 240 250
GIGSSFSSVA GLGMLASVYT DDHERGRAMG TALGGLALGL LVGAPFGSVM
260 270 280 290 300
YEFVGKSAPF LILAFLALLD GALQLCILQP SKVSPESAKG TPLFMLLKDP
310 320 330 340 350
YILVAAGSIC FANMGVAILE PTLPIWMMQT MCSPKWQLGL AFLPASVSYL
360 370 380 390 400
IGTNLFGVLA NKMGRWLCSL IGMLVVGTSL LCVPLAHNIF GLIGPNAGLG
410 420 430 440 450
LAIGMVDSSM MPIMGHLVDL RHTSVYGSVY AIADVAFCMG FAIGPSTGGA
460 470 480 490 500
IVKAIGFPWL MVITGVINIV YAPLCYYLRS PPAKEEKLAI LSQDCPMETR
510 520
MYATQKPTKE FPLGEDSDEE PDHEE
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ96GL6 | Q96GL6_HUMAN | Chromaffin granule amine transporte... | SLC18A1 | 385 | Annotation score: | ||
E5RK12 | E5RK12_HUMAN | Chromaffin granule amine transporte... | SLC18A1 | 258 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_021856 | 4 | T → P1 PublicationCorresponds to variant dbSNP:rs2270641Ensembl. | 1 | |
Natural variantiVAR_052002 | 11 | R → Q. Corresponds to variant dbSNP:rs17092144Ensembl. | 1 | |
Natural variantiVAR_024632 | 74 | A → V. Corresponds to variant dbSNP:rs17215815Ensembl. | 1 | |
Natural variantiVAR_029149 | 82 | F → C. Corresponds to variant dbSNP:rs17215822Ensembl. | 1 | |
Natural variantiVAR_024633 | 84 | F → S. Corresponds to variant dbSNP:rs17215801Ensembl. | 1 | |
Natural variantiVAR_020033 | 98 | S → T. Corresponds to variant dbSNP:rs2270637Ensembl. | 1 | |
Natural variantiVAR_024634 | 101 | A → P. Corresponds to variant dbSNP:rs17222218Ensembl. | 1 | |
Natural variantiVAR_012065 | 136 | I → T1 PublicationCorresponds to variant dbSNP:rs1390938Ensembl. | 1 | |
Natural variantiVAR_029150 | 140 | G → R. Corresponds to variant dbSNP:rs17215808Ensembl. | 1 | |
Natural variantiVAR_024635 | 164 | I → M. Corresponds to variant dbSNP:rs17222092Ensembl. | 1 | |
Natural variantiVAR_029151 | 202 | I → T. Corresponds to variant dbSNP:rs17222120Ensembl. | 1 | |
Natural variantiVAR_024636 | 249 | V → I. Corresponds to variant dbSNP:rs17215759Ensembl. | 1 | |
Natural variantiVAR_024637 | 392 | L → V. Corresponds to variant dbSNP:rs17092104Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046304 | 307 – 338 | Missing in isoform 3. 1 PublicationAdd BLAST | 32 | |
Alternative sequenceiVSP_046305 | 445 – 525 | PSTGG…PDHEE → YSESGLPHGDPDVCNPEAHE GISSGGGQ in isoform 2. 1 PublicationAdd BLAST | 81 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39905 mRNA Translation: AAC50472.1 AC025853 Genomic DNA No translation available. BC006317 mRNA Translation: AAH06317.1 |
CCDSi | CCDS47814.1 [P54219-3] CCDS47815.1 [P54219-2] CCDS6013.1 [P54219-1] |
RefSeqi | NP_001129163.1, NM_001135691.2 [P54219-1] NP_001135796.1, NM_001142324.1 [P54219-3] NP_001135797.1, NM_001142325.1 [P54219-2] NP_003044.1, NM_003053.3 [P54219-1] XP_011542928.1, XM_011544626.1 [P54219-3] XP_011542929.1, XM_011544627.1 |
Genome annotation databases
Ensembli | ENST00000265808; ENSP00000265808; ENSG00000036565 [P54219-3] ENST00000276373; ENSP00000276373; ENSG00000036565 [P54219-1] ENST00000381608; ENSP00000371021; ENSG00000036565 [P54219-2] ENST00000437980; ENSP00000413361; ENSG00000036565 [P54219-2] ENST00000440926; ENSP00000387549; ENSG00000036565 [P54219-1] ENST00000519026; ENSP00000429664; ENSG00000036565 [P54219-3] |
GeneIDi | 6570 |
KEGGi | hsa:6570 |
UCSCi | uc003wzm.3, human [P54219-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39905 mRNA Translation: AAC50472.1 AC025853 Genomic DNA No translation available. BC006317 mRNA Translation: AAH06317.1 |
CCDSi | CCDS47814.1 [P54219-3] CCDS47815.1 [P54219-2] CCDS6013.1 [P54219-1] |
RefSeqi | NP_001129163.1, NM_001135691.2 [P54219-1] NP_001135796.1, NM_001142324.1 [P54219-3] NP_001135797.1, NM_001142325.1 [P54219-2] NP_003044.1, NM_003053.3 [P54219-1] XP_011542928.1, XM_011544626.1 [P54219-3] XP_011542929.1, XM_011544627.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 112458, 127 interactors |
IntActi | P54219, 104 interactors |
STRINGi | 9606.ENSP00000387549 |
Chemistry databases
BindingDBi | P54219 |
ChEMBLi | CHEMBL1838 |
DrugBanki | DB01363, Ephedra sinica root DB01577, Metamfetamine DB01442, MMDA DB00368, Norepinephrine DB00206, Reserpine |
DrugCentrali | P54219 |
GuidetoPHARMACOLOGYi | 1011 |
Protein family/group databases
TCDBi | 2.A.1.2.12, the major facilitator superfamily (mfs) |
PTM databases
GlyGeni | P54219, 3 sites |
iPTMneti | P54219 |
PhosphoSitePlusi | P54219 |
Polymorphism and mutation databases
BioMutai | SLC18A1 |
DMDMi | 1722741 |
Proteomic databases
MassIVEi | P54219 |
PaxDbi | P54219 |
PeptideAtlasi | P54219 |
PRIDEi | P54219 |
ProteomicsDBi | 19679 56654 [P54219-1] |
Protocols and materials databases
Antibodypediai | 1514, 159 antibodies |
DNASUi | 6570 |
Genome annotation databases
Ensembli | ENST00000265808; ENSP00000265808; ENSG00000036565 [P54219-3] ENST00000276373; ENSP00000276373; ENSG00000036565 [P54219-1] ENST00000381608; ENSP00000371021; ENSG00000036565 [P54219-2] ENST00000437980; ENSP00000413361; ENSG00000036565 [P54219-2] ENST00000440926; ENSP00000387549; ENSG00000036565 [P54219-1] ENST00000519026; ENSP00000429664; ENSG00000036565 [P54219-3] |
GeneIDi | 6570 |
KEGGi | hsa:6570 |
UCSCi | uc003wzm.3, human [P54219-1] |
Organism-specific databases
CTDi | 6570 |
DisGeNETi | 6570 |
EuPathDBi | HostDB:ENSG00000036565.14 |
GeneCardsi | SLC18A1 |
HGNCi | HGNC:10934, SLC18A1 |
HPAi | ENSG00000036565, Tissue enhanced (adrenal gland, intestine) |
MIMi | 193002, gene |
neXtProti | NX_P54219 |
OpenTargetsi | ENSG00000036565 |
PharmGKBi | PA324 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3764, Eukaryota |
GeneTreei | ENSGT00940000159352 |
HOGENOMi | CLU_001265_10_9_1 |
InParanoidi | P54219 |
OMAi | RVGYHIP |
PhylomeDBi | P54219 |
TreeFami | TF313494 |
Enzyme and pathway databases
PathwayCommonsi | P54219 |
Reactomei | R-HSA-442660, Na+/Cl- dependent neurotransmitter transporters |
SIGNORi | P54219 |
Miscellaneous databases
BioGRID-ORCSi | 6570, 4 hits in 844 CRISPR screens |
ChiTaRSi | SLC18A1, human |
GenomeRNAii | 6570 |
Pharosi | P54219, Tchem |
PROi | PR:P54219 |
RNActi | P54219, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000036565, Expressed in adrenal gland and 74 other tissues |
ExpressionAtlasi | P54219, baseline and differential |
Genevisiblei | P54219, HS |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR004734, Multidrug-R |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00880, 2_A_01_02, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VMAT1_HUMAN | |
Accessioni | P54219Primary (citable) accession number: P54219 Secondary accession number(s): E9PDJ5, Q9BRE4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations