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Protein

Activated RNA polymerase II transcriptional coactivator p15

Gene

SUB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).7 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SIGNORiP53999

Names & Taxonomyi

Protein namesi
Recommended name:
Activated RNA polymerase II transcriptional coactivator p15
Alternative name(s):
Positive cofactor 4
Short name:
PC4
SUB1 homolog
p14
Gene namesi
Name:SUB1
Synonyms:PC4, RPO2TC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000113387.11
HGNCiHGNC:19985 SUB1
MIMi600503 gene
neXtProtiNX_P53999

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi68K → G: Reduced ssDNA binding. 1 Publication1
Mutagenesisi75R → G: Reduced ssDNA binding. 1 Publication1
Mutagenesisi77 – 80FKGK → AGG: Loss of ssDNA binding. 1 Publication4
Mutagenesisi86R → G: Loss of ssDNA binding. 1 Publication1
Mutagenesisi101K → G: Loss of ssDNA binding. 1 Publication1

Organism-specific databases

DisGeNETi10923
OpenTargetsiENSG00000113387
PharmGKBiPA142670858

Polymorphism and mutation databases

BioMutaiSUB1
DMDMi1709514

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002159442 – 127Activated RNA polymerase II transcriptional coactivator p15Add BLAST126

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineCombined sources1
Modified residuei9PhosphoserineCombined sources1
Modified residuei10PhosphoserineCombined sources1
Modified residuei11Phosphoserine1 Publication1
Modified residuei13PhosphoserineCombined sources1 Publication1
Modified residuei15PhosphoserineCombined sources1 Publication1
Modified residuei17PhosphoserineCombined sources1 Publication1
Modified residuei19PhosphoserineCombined sources1 Publication1
Modified residuei35N6-acetyllysineCombined sources1
Modified residuei53N6-acetyllysineBy similarity1
Modified residuei55PhosphoserineCombined sources1
Modified residuei56PhosphoserineCombined sources1
Modified residuei57PhosphoserineCombined sources1
Modified residuei58PhosphoserineCombined sources1
Modified residuei68N6-acetyllysine; alternateCombined sources1
Cross-linki68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei118PhosphoserineCombined sources1

Post-translational modificationi

Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei50 – 51Cleavage2

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP53999
PaxDbiP53999
PeptideAtlasiP53999
PRIDEiP53999
ProteomicsDBi56641
TopDownProteomicsiP53999

PTM databases

iPTMnetiP53999
PhosphoSitePlusiP53999
SwissPalmiP53999

Expressioni

Gene expression databases

BgeeiENSG00000113387 Expressed in 240 organ(s), highest expression level in epithelium of mammary gland
CleanExiHS_SUB1
ExpressionAtlasiP53999 baseline and differential
GenevisibleiP53999 HS

Organism-specific databases

HPAiCAB015351
HPA001311

Interactioni

Subunit structurei

Homodimer. Interacts with CSTF2.3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116127, 83 interactors
CORUMiP53999
DIPiDIP-29044N
IntActiP53999, 17 interactors
MINTiP53999
STRINGi9606.ENSP00000265073

Structurei

Secondary structure

1127
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP53999
SMRiP53999
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53999

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 50RegulatoryAdd BLAST49
Regioni77 – 101Interaction with ssDNAAdd BLAST25

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 19Ser-richAdd BLAST16
Compositional biasi23 – 53Lys-richAdd BLAST31
Compositional biasi50 – 58Ser-rich9

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2712 Eukaryota
ENOG410XUB8 LUCA
GeneTreeiENSGT00390000008802
HOVERGENiHBG028243
InParanoidiP53999
OMAiMVDIREH
OrthoDBiEOG091G14VO
PhylomeDBiP53999
TreeFamiTF313859

Family and domain databases

Gene3Di2.30.31.10, 1 hit
InterProiView protein in InterPro
IPR003173 PC4
IPR009044 ssDNA-bd_transcriptional_reg
PfamiView protein in Pfam
PF02229 PC4, 1 hit
SUPFAMiSSF54447 SSF54447, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P53999-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPKSKELVSS SSSGSDSDSE VDKKLKRKKQ VAPEKPVKKQ KTGETSRALS
60 70 80 90 100
SSKQSSSSRD DNMFQIGKMR YVSVRDFKGK VLIDIREYWM DPEGEMKPGR
110 120
KGISLNPEQW SQLKEQISDI DDAVRKL
Length:127
Mass (Da):14,395
Last modified:January 23, 2007 - v3
Checksum:i0DAE0CAAAD5E4E15
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RC37D6RC37_HUMAN
Activated RNA polymerase II transcr...
SUB1
65Annotation score:
D6R970D6R970_HUMAN
Activated RNA polymerase II transcr...
SUB1
84Annotation score:

Mass spectrometryi

Molecular mass is 14266±4 Da from positions 2 - 127. Determined by MALDI. 1 Publication
Molecular mass is 14263 Da from positions 2 - 127. Determined by ESI. 1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03287011S → G1 PublicationCorresponds to variant dbSNP:rs17850527Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79805 mRNA Translation: CAA56200.1
U12979 mRNA Translation: AAA20980.1
BC009610 mRNA Translation: AAH09610.1
BC010537 mRNA Translation: AAH10537.1
BC018189 mRNA Translation: AAH18189.1
BC022339 mRNA Translation: AAH22339.1
CCDSiCCDS3897.1
PIRiA54670
RefSeqiNP_006704.3, NM_006713.3
XP_011512246.1, XM_011513944.2
XP_016864475.1, XM_017008986.1
XP_016864476.1, XM_017008987.1
UniGeneiHs.229641

Genome annotation databases

EnsembliENST00000265073; ENSP00000265073; ENSG00000113387
ENST00000502897; ENSP00000427100; ENSG00000113387
ENST00000506237; ENSP00000422078; ENSG00000113387
ENST00000512913; ENSP00000422806; ENSG00000113387
ENST00000515355; ENSP00000426850; ENSG00000113387
GeneIDi10923
KEGGihsa:10923

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79805 mRNA Translation: CAA56200.1
U12979 mRNA Translation: AAA20980.1
BC009610 mRNA Translation: AAH09610.1
BC010537 mRNA Translation: AAH10537.1
BC018189 mRNA Translation: AAH18189.1
BC022339 mRNA Translation: AAH22339.1
CCDSiCCDS3897.1
PIRiA54670
RefSeqiNP_006704.3, NM_006713.3
XP_011512246.1, XM_011513944.2
XP_016864475.1, XM_017008986.1
XP_016864476.1, XM_017008987.1
UniGeneiHs.229641

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PCFX-ray1.74A/B/C/D/E/F/G/H63-127[»]
2C62X-ray1.74A/B63-127[»]
2PHENMR-A/B63-127[»]
4USGX-ray1.97A/B63-127[»]
ProteinModelPortaliP53999
SMRiP53999
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116127, 83 interactors
CORUMiP53999
DIPiDIP-29044N
IntActiP53999, 17 interactors
MINTiP53999
STRINGi9606.ENSP00000265073

PTM databases

iPTMnetiP53999
PhosphoSitePlusiP53999
SwissPalmiP53999

Polymorphism and mutation databases

BioMutaiSUB1
DMDMi1709514

Proteomic databases

EPDiP53999
PaxDbiP53999
PeptideAtlasiP53999
PRIDEiP53999
ProteomicsDBi56641
TopDownProteomicsiP53999

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265073; ENSP00000265073; ENSG00000113387
ENST00000502897; ENSP00000427100; ENSG00000113387
ENST00000506237; ENSP00000422078; ENSG00000113387
ENST00000512913; ENSP00000422806; ENSG00000113387
ENST00000515355; ENSP00000426850; ENSG00000113387
GeneIDi10923
KEGGihsa:10923

Organism-specific databases

CTDi10923
DisGeNETi10923
EuPathDBiHostDB:ENSG00000113387.11
GeneCardsiSUB1
HGNCiHGNC:19985 SUB1
HPAiCAB015351
HPA001311
MIMi600503 gene
neXtProtiNX_P53999
OpenTargetsiENSG00000113387
PharmGKBiPA142670858
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2712 Eukaryota
ENOG410XUB8 LUCA
GeneTreeiENSGT00390000008802
HOVERGENiHBG028243
InParanoidiP53999
OMAiMVDIREH
OrthoDBiEOG091G14VO
PhylomeDBiP53999
TreeFamiTF313859

Enzyme and pathway databases

SIGNORiP53999

Miscellaneous databases

ChiTaRSiSUB1 human
EvolutionaryTraceiP53999
GeneWikiiSUB1
GenomeRNAii10923
PROiPR:P53999
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000113387 Expressed in 240 organ(s), highest expression level in epithelium of mammary gland
CleanExiHS_SUB1
ExpressionAtlasiP53999 baseline and differential
GenevisibleiP53999 HS

Family and domain databases

Gene3Di2.30.31.10, 1 hit
InterProiView protein in InterPro
IPR003173 PC4
IPR009044 ssDNA-bd_transcriptional_reg
PfamiView protein in Pfam
PF02229 PC4, 1 hit
SUPFAMiSSF54447 SSF54447, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTCP4_HUMAN
AccessioniPrimary (citable) accession number: P53999
Secondary accession number(s): Q96L29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 171 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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