UniProtKB - P53995 (APC1_MOUSE)
Anaphase-promoting complex subunit 1
Anapc1
Functioni
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).
By similarity: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- molecular adaptor activity Source: GO_Central
GO - Biological processi
- anaphase-promoting complex-dependent catabolic process Source: GO_Central
- cell division Source: UniProtKB-KW
- metaphase/anaphase transition of mitotic cell cycle Source: GO_Central
- protein K11-linked ubiquitination Source: UniProtKB
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis, Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-141430, Inactivation of APC/C via direct inhibition of the APC/C complex R-MMU-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-MMU-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-MMU-174154, APC/C:Cdc20 mediated degradation of Securin R-MMU-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-176407, Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase R-MMU-176408, Regulation of APC/C activators between G1/S and early anaphase R-MMU-176409, APC/C:Cdc20 mediated degradation of mitotic proteins R-MMU-176412, Phosphorylation of the APC/C R-MMU-179409, APC-Cdc20 mediated degradation of Nek2A R-MMU-2467813, Separation of Sister Chromatids R-MMU-2559582, Senescence-Associated Secretory Phenotype (SASP) R-MMU-69017, CDK-mediated phosphorylation and removal of Cdc6 R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Anaphase-promoting complex subunit 1Short name: APC1 Alternative name(s): Cyclosome subunit 1 Mitotic checkpoint regulator Testis-specific gene 24 protein |
Gene namesi | Name:Anapc1 Synonyms:Mcpr, Tsg24 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:103097, Anapc1 |
VEuPathDBi | HostDB:ENSMUSG00000014355 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215872 | 1 – 1944 | Anaphase-promoting complex subunit 1Add BLAST | 1944 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 51 | PhosphoserineBy similarity | 1 | |
Modified residuei | 60 | PhosphoserineBy similarity | 1 | |
Modified residuei | 291 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 313 | PhosphoserineBy similarity | 1 | |
Modified residuei | 341 | PhosphoserineBy similarity | 1 | |
Modified residuei | 343 | PhosphoserineBy similarity | 1 | |
Modified residuei | 355 | PhosphoserineBy similarity | 1 | |
Modified residuei | 362 | PhosphoserineBy similarity | 1 | |
Modified residuei | 373 | PhosphoserineBy similarity | 1 | |
Modified residuei | 377 | PhosphoserineCombined sources | 1 | |
Modified residuei | 537 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 547 | PhosphoserineCombined sources | 1 | |
Modified residuei | 555 | PhosphoserineBy similarity | 1 | |
Modified residuei | 571 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 680 | PhosphoserineCombined sources | 1 | |
Modified residuei | 686 | PhosphoserineBy similarity | 1 | |
Modified residuei | 688 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P53995 |
jPOSTi | P53995 |
MaxQBi | P53995 |
PaxDbi | P53995 |
PRIDEi | P53995 |
ProteomicsDBi | 281825 |
PTM databases
iPTMneti | P53995 |
PhosphoSitePlusi | P53995 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000014355, Expressed in uterus and 312 other tissues |
ExpressionAtlasi | P53995, baseline and differential |
Genevisiblei | P53995, MM |
Interactioni
Subunit structurei
The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5.
By similarityProtein-protein interaction databases
BioGRIDi | 201352, 22 interactors |
CORUMi | P53995 |
IntActi | P53995, 1 interactor |
STRINGi | 10090.ENSMUSP00000014499 |
Miscellaneous databases
RNActi | P53995, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 1297 – 1325 | PC 1Add BLAST | 29 | |
Repeati | 1366 – 1404 | PC 2Add BLAST | 39 | |
Repeati | 1467 – 1501 | PC 3Add BLAST | 35 | |
Repeati | 1520 – 1552 | PC 4Add BLAST | 33 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 312 – 343 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 370 – 395 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 991 – 1014 | DisorderedSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1858, Eukaryota |
GeneTreei | ENSGT00390000016757 |
HOGENOMi | CLU_001202_1_0_1 |
InParanoidi | P53995 |
OMAi | MQPPDSR |
OrthoDBi | 738153at2759 |
PhylomeDBi | P53995 |
TreeFami | TF105441 |
Family and domain databases
Gene3Di | 1.25.10.10, 2 hits |
InterProi | View protein in InterPro IPR024990, Apc1 IPR041221, APC1_C IPR011989, ARM-like IPR002015, Proteasome/cyclosome_rpt |
PANTHERi | PTHR12827, PTHR12827, 1 hit |
Pfami | View protein in Pfam PF12859, ANAPC1, 1 hit PF18122, APC1_C, 1 hit PF01851, PC_rep, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSNFSEERAT MIAAGDLQEF VPFGRDHCKH HPNALNLQLR QLQPASELWS
60 70 80 90 100
SDGAAGLVGS LQEVTIHEKQ KESWQLRKGV SEIGDAADYD EELYVAGNMV
110 120 130 140 150
IWSKGSKSQA LAVYKAFTVD STVQQALWCD FIISQDKSEK IYKSHELEKC
160 170 180 190 200
ICILQSSCMN MHSIDGKDYI ASLPFQVANV WATKYGLLFE RCSSSHEVPP
210 220 230 240 250
SLPREPLPTM FSMLHPLDEI TPLVCKSGSL FGSSRVQYVV DPAVKIVFLN
260 270 280 290 300
IDPSIVMTYD AVQNVHSVWT LRRVKPEEEN AVLKFPEQAG TLQNATTSSS
310 320 330 340 350
LTAHLRSLSK GESPVASPFQ NYSSIHSQSR STSSPSLHSR SPSISNMAAL
360 370 380 390 400
SRAHSPALGV HSFSGAQRFN LSSHSQSPKR HSISHSPSGS FNDSFLAPET
410 420 430 440 450
EPIVPELCID HLWTETLPNI REKNSQASKV FITTDLCGQK FLCFLVEAQL
460 470 480 490 500
QLRCVKFQES NDKTQLIFGS VTNIHAKDAA PVEKIHTMLV LEGNGNLVLY
510 520 530 540 550
TGVVRVGKVF IPGLPAPSLT MSNMMPRPST PLDGVGTPKP LSKLLGSMDE
560 570 580 590 600
VVLLSPVPEL RDSSKLNDSL YNEDCTFQQL GTYIHSVRDP VHNRVTLELS
610 620 630 640 650
NGSMVRITIP EVATSELVQT CLQAIKFILP KEVAIQVLVK WYNVHSAPGG
660 670 680 690 700
PSCHSEWSLF VICLLNMMGY NTDRLAWTRS FDFEGSLSPV IAPKKARPSD
710 720 730 740 750
TGSDEDWEYL LNSEYHRNVE SHLLNKSLCL TALEVSNAKD EDFSQNLSLD
760 770 780 790 800
SSTLLFAHIP AIFFVLHLVY EELKLNTLMG EGICSLIDLL VQLARDLKLD
810 820 830 840 850
SYLDHYYRDS PTLVKTTGQV CTIDQGQMGF MHHPPFFTSE PPSIYQWVSS
860 870 880 890 900
CLKGEGMPPY PYLPGICERS RLVVLSIALY TLGDESCVSD ETCQYLSKVT
910 920 930 940 950
STPQKPQAEQ EENRFTFRHS ASVSVLAERL VVWMASVGFT LRDLETLPFG
960 970 980 990 1000
IALPIRDAIY HCREQPDSDW SEAVCLLIGR QDLSKQACEG NLPRGKSVLS
1010 1020 1030 1040 1050
SEVSSGTEAE EEDDGMNDLN HEVMSLIWSE DLRVQDVRRL LQSAQPVRVN
1060 1070 1080 1090 1100
VVQYPELSDH EFIEEKENRL LQLCQRTMAL PVGRGMFTLF SYHPVPTEPL
1110 1120 1130 1140 1150
PVPKLNLTGR APPRNTTVDL NSGNIDVPPN MASWASFHNG VAAGLKIAPA
1160 1170 1180 1190 1200
SQIDSAWIVY NKPKHAELAN EYAGFLMALG LNGHLTKLAT LNIHDYLTKG
1210 1220 1230 1240 1250
HEMTSIGLLL GVSAAKLGTM DMSITRLLSI HVPALLPPTS TELDVPHNVQ
1260 1270 1280 1290 1300
VAAVVGIGLV YQGTAHRHTA EVLLAEIGRP PGPEMEYCTD RESYSLAAGL
1310 1320 1330 1340 1350
ALGMVCLGHG SNLIGMSDLN VPEQLYQYMV GGHRRFQTGM HREKHKSPSY
1360 1370 1380 1390 1400
QIKEGDTINV DVTCPGATLA LAMIYLKTNN RSIADWLRAP DTMYLLDFVK
1410 1420 1430 1440 1450
PEFLLLRTLA RCLILWDDIL PNSKWVDSNV PQIIRENSIS LSEIELPCSE
1460 1470 1480 1490 1500
DLNLETLSQA HVYIIAGACL SLGFRFAGSE NLSAFSCLHK FAKDFMNYLS
1510 1520 1530 1540 1550
APNASVTGPY NLETCLSVVL LSLAMVMAGS GNLKVLQLCR FLHMKTGGEM
1560 1570 1580 1590 1600
NYGFHLAHHM ALGLLFLGGG RYSLSTSNSS IAALLCALYP HFPAHSTDNR
1610 1620 1630 1640 1650
YHLQALRHLY VLAAEPRLLV PVDVDTNTPC YALIEVTYKG TQWYEQTKEE
1660 1670 1680 1690 1700
LMAPTLLPEL HLLKQMKVKG PRYWELLIDL SKGEQHLRSI LSKDGVLYVK
1710 1720 1730 1740 1750
LRAGQLSYKE DPMGWQSLLA QTVANRNSEA RAFKPETISS FTSDPALLSF
1760 1770 1780 1790 1800
AEYFCKPTVS MGPKQEILDL FSSILYECVA QETPEMLPAY IAMDQALRSL
1810 1820 1830 1840 1850
KKRDMSDTSD LWQIKLILEF FSSRSHQDRQ HTYPKRGLFI NSEFLPVVKC
1860 1870 1880 1890 1900
TVDATLDQWL QAGGDVCVHA YLSGQPVEKS QLNMLACFLV YHSVPAPRHL
1910 1920 1930 1940
PPMGLEGSTS FAELLYRFRH LKMPVRALLR LAPVLLGNPQ PMVM
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2ATQ5 | A2ATQ5_MOUSE | Anaphase-promoting complex subunit ... | Anapc1 | 1,213 | Annotation score: | ||
Q6PG65 | Q6PG65_MOUSE | Anapc1 protein | Anapc1 | 80 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 112 | A → Q in CAA56450 (PubMed:7929068).Curated | 1 | |
Sequence conflicti | 348 – 349 | AA → GV in CAA56450 (PubMed:7929068).Curated | 2 | |
Sequence conflicti | 643 | N → K in BAC34976 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1036 | D → H in CAA56450 (PubMed:7929068).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X80169 mRNA Translation: CAA56450.1 AL928910 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL28216.1 BC139002 mRNA Translation: AAI39003.1 AK052404 mRNA Translation: BAC34976.1 AK077847 mRNA Translation: BAC37032.2 |
CCDSi | CCDS16715.1 |
PIRi | A55117 |
RefSeqi | NP_032595.2, NM_008569.2 |
Genome annotation databases
Ensembli | ENSMUST00000014499; ENSMUSP00000014499; ENSMUSG00000014355 |
GeneIDi | 17222 |
KEGGi | mmu:17222 |
UCSCi | uc008mgq.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X80169 mRNA Translation: CAA56450.1 AL928910 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL28216.1 BC139002 mRNA Translation: AAI39003.1 AK052404 mRNA Translation: BAC34976.1 AK077847 mRNA Translation: BAC37032.2 |
CCDSi | CCDS16715.1 |
PIRi | A55117 |
RefSeqi | NP_032595.2, NM_008569.2 |
3D structure databases
AlphaFoldDBi | P53995 |
SMRi | P53995 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201352, 22 interactors |
CORUMi | P53995 |
IntActi | P53995, 1 interactor |
STRINGi | 10090.ENSMUSP00000014499 |
PTM databases
iPTMneti | P53995 |
PhosphoSitePlusi | P53995 |
Proteomic databases
EPDi | P53995 |
jPOSTi | P53995 |
MaxQBi | P53995 |
PaxDbi | P53995 |
PRIDEi | P53995 |
ProteomicsDBi | 281825 |
Protocols and materials databases
Antibodypediai | 18056, 544 antibodies from 36 providers |
DNASUi | 17222 |
Genome annotation databases
Ensembli | ENSMUST00000014499; ENSMUSP00000014499; ENSMUSG00000014355 |
GeneIDi | 17222 |
KEGGi | mmu:17222 |
UCSCi | uc008mgq.2, mouse |
Organism-specific databases
CTDi | 64682 |
MGIi | MGI:103097, Anapc1 |
VEuPathDBi | HostDB:ENSMUSG00000014355 |
Phylogenomic databases
eggNOGi | KOG1858, Eukaryota |
GeneTreei | ENSGT00390000016757 |
HOGENOMi | CLU_001202_1_0_1 |
InParanoidi | P53995 |
OMAi | MQPPDSR |
OrthoDBi | 738153at2759 |
PhylomeDBi | P53995 |
TreeFami | TF105441 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-141430, Inactivation of APC/C via direct inhibition of the APC/C complex R-MMU-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-MMU-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-MMU-174154, APC/C:Cdc20 mediated degradation of Securin R-MMU-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-176407, Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase R-MMU-176408, Regulation of APC/C activators between G1/S and early anaphase R-MMU-176409, APC/C:Cdc20 mediated degradation of mitotic proteins R-MMU-176412, Phosphorylation of the APC/C R-MMU-179409, APC-Cdc20 mediated degradation of Nek2A R-MMU-2467813, Separation of Sister Chromatids R-MMU-2559582, Senescence-Associated Secretory Phenotype (SASP) R-MMU-69017, CDK-mediated phosphorylation and removal of Cdc6 R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 17222, 22 hits in 72 CRISPR screens |
ChiTaRSi | Anapc1, mouse |
PROi | PR:P53995 |
RNActi | P53995, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000014355, Expressed in uterus and 312 other tissues |
ExpressionAtlasi | P53995, baseline and differential |
Genevisiblei | P53995, MM |
Family and domain databases
Gene3Di | 1.25.10.10, 2 hits |
InterProi | View protein in InterPro IPR024990, Apc1 IPR041221, APC1_C IPR011989, ARM-like IPR002015, Proteasome/cyclosome_rpt |
PANTHERi | PTHR12827, PTHR12827, 1 hit |
Pfami | View protein in Pfam PF12859, ANAPC1, 1 hit PF18122, APC1_C, 1 hit PF01851, PC_rep, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | APC1_MOUSE | |
Accessioni | P53995Primary (citable) accession number: P53995 Secondary accession number(s): A2ATQ4, Q8BP33, Q8C772 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | July 27, 2011 | |
Last modified: | May 25, 2022 | |
This is version 146 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families