UniProtKB - P53990 (IST1_HUMAN)
IST1 homolog
IST1
Functioni
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
- identical protein binding Source: IntAct
- MIT domain binding Source: UniProtKB
- protein-containing complex binding Source: UniProtKB
- protein domain specific binding Source: UniProtKB
GO - Biological processi
- abscission Source: UniProtKB
- cell division Source: UniProtKB
- cytoskeleton-dependent cytokinesis Source: UniProtKB
- ESCRT III complex disassembly Source: ParkinsonsUK-UCL
- establishment of protein localization Source: UniProtKB
- mitotic nuclear envelope reassembly Source: Reactome
- multivesicular body assembly Source: ParkinsonsUK-UCL
- neutrophil degranulation Source: Reactome
- positive regulation of collateral sprouting Source: Ensembl
- positive regulation of proteolysis Source: UniProtKB
- protein localization Source: UniProtKB
- protein transport Source: InterPro
- viral capsid secondary envelopment Source: UniProtKB
- viral release from host cell Source: UniProtKB
Keywordsi
Biological process | Cell cycle, Cell division |
Enzyme and pathway databases
PathwayCommonsi | P53990 |
Reactomei | R-HSA-6798695, Neutrophil degranulation R-HSA-9668328, Sealing of the nuclear envelope (NE) by ESCRT-III |
Names & Taxonomyi
Protein namesi | Recommended name: IST1 homologShort name: hIST1 Alternative name(s): Putative MAPK-activating protein PM28 |
Gene namesi | Name:IST1 Synonyms:KIAA0174 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28977, IST1 |
MIMi | 616434, gene |
neXtProti | NX_P53990 |
VEuPathDBi | HostDB:ENSG00000182149.20 |
Subcellular locationi
Nucleus
- Nucleus envelope 1 Publication
Cytoskeleton
- centrosome 1 Publication
Other locations
- Cytoplasmic vesicle 2 Publications
- Midbody 1 Publication
Note: Localizes to centrosome and midbody of dividing cells (PubMed:19129480, PubMed:19129479, PubMed:20719964). Colocalized with SPART to the ends of Flemming bodies during cytokinesis (PubMed:20719964). Localizes to the nuclear envelope during late anaphase (PubMed:26040712).4 Publications
Cytoskeleton
- centrosome Source: UniProtKB
Cytosol
- cytosol Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular region Source: Reactome
Lysosome
- azurophil granule lumen Source: Reactome
Nucleus
- nuclear envelope Source: UniProtKB-SubCell
Other locations
- endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
- Flemming body Source: UniProtKB
- intracellular membrane-bounded organelle Source: HPA
- midbody Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 323 | L → D: Diminishes interaction with VPS4A. Greatly diminishes interaction with VPS4A; when associated with A-353. 1 Publication | 1 | |
Mutagenesisi | 326 | L → D: Diminishes interaction with VPS4A. Greatly diminishes interaction with VPS4A and abolishes interaction with VTA1; when associated with A-353. Greatly diminishes interaction with VPS4A; when associated with A-360. 1 Publication | 1 | |
Mutagenesisi | 353 | L → A: Diminishes interaction with VPS4A. Greatly diminishes interaction with VPS4A and abolishes interaction with VTA1; when associated with D-326. Greatly diminishes interaction with VPS4A; when associated with D-323. 1 Publication | 1 | |
Mutagenesisi | 360 – 361 | LK → AA: Abolishes interaction with VTA1, MITD1 and USP8; diminishes interaction with VPS4A. 1 Publication | 2 | |
Mutagenesisi | 360 | L → A: Diminishes interaction with VPS4A. Greatly diminishes interaction with VPS4A; when associated with D-326. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 9798 |
OpenTargetsi | ENSG00000182149 |
PharmGKBi | PA142671633 |
Miscellaneous databases
Pharosi | P53990, Tbio |
Genetic variation databases
BioMutai | IST1 |
DMDMi | 1723119 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050727 | 1 – 364 | IST1 homologAdd BLAST | 364 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 4 | PhosphoserineCombined sources | 1 | |
Modified residuei | 43 | PhosphotyrosineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P53990 |
jPOSTi | P53990 |
MassIVEi | P53990 |
MaxQBi | P53990 |
PaxDbi | P53990 |
PeptideAtlasi | P53990 |
PRIDEi | P53990 |
ProteomicsDBi | 1902 56636 [P53990-1] 56637 [P53990-2] 56638 [P53990-3] 56639 [P53990-4] |
PTM databases
iPTMneti | P53990 |
MetOSitei | P53990 |
PhosphoSitePlusi | P53990 |
SwissPalmi | P53990 |
Expressioni
Gene expression databases
Bgeei | ENSG00000182149, Expressed in left lobe of thyroid gland and 246 other tissues |
ExpressionAtlasi | P53990, baseline and differential |
Genevisiblei | P53990, HS |
Organism-specific databases
HPAi | ENSG00000182149, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with CHMP1A, CHMP1B, VPS4A and VTA1.
Interacts with SPAST, STAMBP, and USP8. May interact with VPS37B. May associate with the ESCRT-I complex.
Interacts with MITD1, in competition with VSP4 (PubMed:23015756).
Interacts with SPART (via MIT domain); leading to the recruitment of SPART to midbodies (PubMed:20719964).
Interacts with SPAST (PubMed:23897888, PubMed:26040712).
6 PublicationsBinary interactionsi
Hide detailsP53990
With | #Exp. | IntAct |
---|---|---|
APEH [P13798] | 5 | EBI-945994,EBI-723792 |
ATXN1 [P54253] | 2 | EBI-945994,EBI-930964 |
CAPN7 [Q7Z479] | 3 | EBI-945994,EBI-10213454 |
CAPN7 [Q9Y6W3] | 6 | EBI-945994,EBI-1765641 |
UBQLN1 [Q9UMX0] | 3 | EBI-945994,EBI-741480 |
Isoform 2 [P53990-2]
With | #Exp. | IntAct |
---|---|---|
ATXN1 [P54253] | 6 | EBI-4402971,EBI-930964 |
Isoform 3 [P53990-3]
IST1 - isoform 4 [P53990-4]
With | #Exp. | IntAct |
---|---|---|
CHMP1B [Q7LBR1] | 3 | EBI-15788863,EBI-2118090 |
itself | 2 | EBI-15788863,EBI-15788863 |
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
- identical protein binding Source: IntAct
- MIT domain binding Source: UniProtKB
- protein domain specific binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 115141, 62 interactors |
DIPi | DIP-42546N |
IntActi | P53990, 38 interactors |
MINTi | P53990 |
STRINGi | 9606.ENSP00000438399 |
Miscellaneous databases
RNActi | P53990, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P53990 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P53990 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 168 | Interaction with CHMP1A and CHMP1BAdd BLAST | 168 | |
Regioni | 64 – 279 | Interaction with VPS37B1 PublicationAdd BLAST | 216 | |
Regioni | 190 – 364 | Interaction with VTA1Add BLAST | 175 | |
Regioni | 348 – 364 | Interaction with VPS4A, VTA1, MITD1 STAMBP and USP81 PublicationAdd BLAST | 17 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 321 – 332 | Type-2 MIT-interacting motifAdd BLAST | 12 | |
Motifi | 351 – 361 | MIT-interacting motifAdd BLAST | 11 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2027, Eukaryota |
GeneTreei | ENSGT00390000007453 |
InParanoidi | P53990 |
OMAi | PQPRSKM |
OrthoDBi | 1302348at2759 |
PhylomeDBi | P53990 |
TreeFami | TF314258 |
Family and domain databases
Gene3Di | 1.20.1260.60, 1 hit |
InterProi | View protein in InterPro IPR005061, Ist1 IPR042277, IST1-like |
PANTHERi | PTHR12161, PTHR12161, 1 hit |
Pfami | View protein in Pfam PF03398, Ist1, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLGSGFKAER LRVNLRLVIN RLKLLEKKKT ELAQKARKEI ADYLAAGKDE
60 70 80 90 100
RARIRVEHII REDYLVEAME ILELYCDLLL ARFGLIQSMK ELDSGLAESV
110 120 130 140 150
STLIWAAPRL QSEVAELKIV ADQLCAKYSK EYGKLCRTNQ IGTVNDRLMH
160 170 180 190 200
KLSVEAPPKI LVERYLIEIA KNYNVPYEPD SVVMAEAPPG VETDLIDVGF
210 220 230 240 250
TDDVKKGGPG RGGSGGFTAP VGGPDGTVPM PMPMPMPSAN TPFSYPLPKG
260 270 280 290 300
PSDFNGLPMG TYQAFPNIHP PQIPATPPSY ESVDDINADK NISSAQIVGP
310 320 330 340 350
GPKPEASAKL PSRPADNYDN FVLPELPSVP DTLPTASAGA STSASEDIDF
360
DDLSRRFEEL KKKT
Computationally mapped potential isoform sequencesi
There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BUI0 | H3BUI0_HUMAN | IST1 homolog | IST1 | 253 | Annotation score: | ||
F5GXM3 | F5GXM3_HUMAN | IST1 homolog | IST1 | 141 | Annotation score: | ||
H3BMU1 | H3BMU1_HUMAN | IST1 homolog | IST1 | 206 | Annotation score: | ||
H3BRE2 | H3BRE2_HUMAN | IST1 homolog | IST1 | 105 | Annotation score: | ||
H3BQF7 | H3BQF7_HUMAN | IST1 homolog | IST1 | 166 | Annotation score: | ||
H3BPP6 | H3BPP6_HUMAN | IST1 homolog | IST1 | 59 | Annotation score: | ||
H3BQ38 | H3BQ38_HUMAN | IST1 homolog | IST1 | 40 | Annotation score: | ||
J3KR23 | J3KR23_HUMAN | IST1 homolog | IST1 | 289 | Annotation score: | ||
E9PF00 | E9PF00_HUMAN | IST1 homolog | IST1 | 41 | Annotation score: | ||
J3QQP8 | J3QQP8_HUMAN | IST1 homolog | IST1 | 85 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055118 | 1 – 148 | Missing in isoform 6. CuratedAdd BLAST | 148 | |
Alternative sequenceiVSP_047075 | 1 | M → MVFKLKTKEEQHSM in isoform 5. 1 Publication | 1 | |
Alternative sequenceiVSP_017118 | 228 | V → VPM in isoform 2, isoform 3, isoform 4 and isoform 6. 2 Publications | 1 | |
Alternative sequenceiVSP_047076 | 237 | P → PMP in isoform 5. 1 Publication | 1 | |
Alternative sequenceiVSP_017119 | 252 – 282 | Missing in isoform 2. 1 PublicationAdd BLAST | 31 | |
Alternative sequenceiVSP_017120 | 283 – 364 | VDDIN…LKKKT → MTLMLIRISLLHRLLVLDPS QKPLQSFLPDLQITMTTLSY QSCHLCQTHYQLHLLVPAPQ HLKTLTLMIFPGGLKS in isoform 3. 1 PublicationAdd BLAST | 82 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB097052 mRNA Translation: BAC77405.1 D79996 mRNA Translation: BAA11491.2 Different initiation. AK057258 mRNA Translation: BAG51894.1 AK293022 mRNA Translation: BAF85711.1 AK293040 mRNA Translation: BAF85729.1 AC009127 Genomic DNA No translation available. AC010653 Genomic DNA No translation available. BC000116 mRNA Translation: AAH00116.1 BC000430 mRNA Translation: AAH00430.1 BC004359 mRNA Translation: AAH04359.1 BC103745 mRNA Translation: AAI03746.1 |
CCDSi | CCDS10905.1 [P53990-3] CCDS59271.1 [P53990-5] CCDS59272.1 [P53990-4] CCDS59273.1 [P53990-2] CCDS59274.1 [P53990-6] |
RefSeqi | NP_001257904.1, NM_001270975.1 [P53990-4] NP_001257905.1, NM_001270976.1 [P53990-5] NP_001257906.1, NM_001270977.1 [P53990-2] NP_001257907.1, NM_001270978.1 [P53990-6] NP_055576.2, NM_014761.3 [P53990-3] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB097052 mRNA Translation: BAC77405.1 D79996 mRNA Translation: BAA11491.2 Different initiation. AK057258 mRNA Translation: BAG51894.1 AK293022 mRNA Translation: BAF85711.1 AK293040 mRNA Translation: BAF85729.1 AC009127 Genomic DNA No translation available. AC010653 Genomic DNA No translation available. BC000116 mRNA Translation: AAH00116.1 BC000430 mRNA Translation: AAH00430.1 BC004359 mRNA Translation: AAH04359.1 BC103745 mRNA Translation: AAI03746.1 |
CCDSi | CCDS10905.1 [P53990-3] CCDS59271.1 [P53990-5] CCDS59272.1 [P53990-4] CCDS59273.1 [P53990-2] CCDS59274.1 [P53990-6] |
RefSeqi | NP_001257904.1, NM_001270975.1 [P53990-4] NP_001257905.1, NM_001270976.1 [P53990-5] NP_001257906.1, NM_001270977.1 [P53990-2] NP_001257907.1, NM_001270978.1 [P53990-6] NP_055576.2, NM_014761.3 [P53990-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FRR | X-ray | 1.80 | A | 1-189 | [»] | |
3FRS | X-ray | 2.61 | A | 5-189 | [»] | |
3JC1 | electron microscopy | 4.00 | Aa/Ac/Ae/Ag/Ai/Ak/Am/Ao/Aq/As/Au/Aw/Ay/Ba/Bc/Be/Bg/Bi/Bk/Bm/Bo/Bq/Bs/Bu/Bw/By/Ca/Cc/Ce/Cg | 6-187 | [»] | |
4U7E | X-ray | 1.60 | A | 341-364 | [»] | |
4U7I | X-ray | 1.79 | B | 341-364 | [»] | |
4U7Y | X-ray | 2.50 | B | 341-364 | [»] | |
4WZX | X-ray | 1.39 | E | 342-364 | [»] | |
6E8G | electron microscopy | 2.90 | A/BA/BB/C/DA/DB/E/FA/FB/G/HA/HB/I/JA/JB/K/LA/LB/M/NA/NB/O/PA/PB/Q/RA/RB/S/TA/TB | 1-364 | [»] | |
6TZ4 | electron microscopy | 3.20 | 01/AA/AB/B/CA/CB/D/EA/EB/F/GA/GB/H/IA/IB/J/KA/KB/L/MA/MB/N/OA/OB/P/QA/QB/R/SA/SB | 1-189 | [»] | |
6TZ5 | electron microscopy | 3.10 | A/BA/BB/C/DA/DB/E/FA/FB/G/HA/HB/I/JA/JB/K/LA/LB/M/NA/NB/O/PA/PB/Q/RA/S/TA/V/VA | 1-189 | [»] | |
6TZA | electron microscopy | 7.20 | A/B/C/D/E/F/G/H/I/J/K/L/M/N | 1-189 | [»] | |
SMRi | P53990 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115141, 62 interactors |
DIPi | DIP-42546N |
IntActi | P53990, 38 interactors |
MINTi | P53990 |
STRINGi | 9606.ENSP00000438399 |
PTM databases
iPTMneti | P53990 |
MetOSitei | P53990 |
PhosphoSitePlusi | P53990 |
SwissPalmi | P53990 |
Genetic variation databases
BioMutai | IST1 |
DMDMi | 1723119 |
Proteomic databases
EPDi | P53990 |
jPOSTi | P53990 |
MassIVEi | P53990 |
MaxQBi | P53990 |
PaxDbi | P53990 |
PeptideAtlasi | P53990 |
PRIDEi | P53990 |
ProteomicsDBi | 1902 56636 [P53990-1] 56637 [P53990-2] 56638 [P53990-3] 56639 [P53990-4] |
Protocols and materials databases
Antibodypediai | 44614, 128 antibodies |
DNASUi | 9798 |
Genome annotation databases
Organism-specific databases
CTDi | 9798 |
DisGeNETi | 9798 |
GeneCardsi | IST1 |
HGNCi | HGNC:28977, IST1 |
HPAi | ENSG00000182149, Low tissue specificity |
MIMi | 616434, gene |
neXtProti | NX_P53990 |
OpenTargetsi | ENSG00000182149 |
PharmGKBi | PA142671633 |
VEuPathDBi | HostDB:ENSG00000182149.20 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2027, Eukaryota |
GeneTreei | ENSGT00390000007453 |
InParanoidi | P53990 |
OMAi | PQPRSKM |
OrthoDBi | 1302348at2759 |
PhylomeDBi | P53990 |
TreeFami | TF314258 |
Enzyme and pathway databases
PathwayCommonsi | P53990 |
Reactomei | R-HSA-6798695, Neutrophil degranulation R-HSA-9668328, Sealing of the nuclear envelope (NE) by ESCRT-III |
Miscellaneous databases
BioGRID-ORCSi | 9798, 88 hits in 994 CRISPR screens |
ChiTaRSi | IST1, human |
EvolutionaryTracei | P53990 |
GenomeRNAii | 9798 |
Pharosi | P53990, Tbio |
PROi | PR:P53990 |
RNActi | P53990, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000182149, Expressed in left lobe of thyroid gland and 246 other tissues |
ExpressionAtlasi | P53990, baseline and differential |
Genevisiblei | P53990, HS |
Family and domain databases
Gene3Di | 1.20.1260.60, 1 hit |
InterProi | View protein in InterPro IPR005061, Ist1 IPR042277, IST1-like |
PANTHERi | PTHR12161, PTHR12161, 1 hit |
Pfami | View protein in Pfam PF03398, Ist1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IST1_HUMAN | |
Accessioni | P53990Primary (citable) accession number: P53990 Secondary accession number(s): A8KAH5 Q9BWN2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families