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Protein

Monocarboxylate transporter 1

Gene

SLC16A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.1 Publication

Miscellaneous

Overexpression in pancreatic beta-cells triggers insulin secretion in response to pyruvate, causing hyperinsulemia and hypoglycemia during strenuous exercise.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processSymport, Transport

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000155380-MONOMER
ReactomeiR-HSA-210991 Basigin interactions
R-HSA-433692 Proton-coupled monocarboxylate transport
R-HSA-5619070 Defective SLC16A1 causes symptomatic deficiency in lactate transport (SDLT)
R-HSA-70268 Pyruvate metabolism
SABIO-RKiP53985

Protein family/group databases

TCDBi2.A.1.13.1 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Monocarboxylate transporter 1
Short name:
MCT 1
Alternative name(s):
Solute carrier family 16 member 1
Gene namesi
Name:SLC16A1
Synonyms:MCT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000155380.11
HGNCiHGNC:10922 SLC16A1
MIMi600682 gene
neXtProtiNX_P53985

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicSequence analysisAdd BLAST15
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Topological domaini37 – 59ExtracellularSequence analysisAdd BLAST23
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Topological domaini81 – 86CytoplasmicSequence analysis6
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Topological domaini108 – 111ExtracellularSequence analysis4
Transmembranei112 – 132HelicalSequence analysisAdd BLAST21
Topological domaini133 – 143CytoplasmicSequence analysisAdd BLAST11
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Topological domaini165 – 166ExtracellularSequence analysis2
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Topological domaini188 – 262CytoplasmicSequence analysisAdd BLAST75
Transmembranei263 – 283HelicalSequence analysisAdd BLAST21
Topological domaini284 – 298ExtracellularSequence analysisAdd BLAST15
Transmembranei299 – 319HelicalSequence analysisAdd BLAST21
Topological domaini320 – 328CytoplasmicSequence analysis9
Transmembranei329 – 349HelicalSequence analysisAdd BLAST21
Topological domaini350 – 353ExtracellularSequence analysis4
Transmembranei354 – 374HelicalSequence analysisAdd BLAST21
Topological domaini375 – 389CytoplasmicSequence analysisAdd BLAST15
Transmembranei390 – 410HelicalSequence analysisAdd BLAST21
Topological domaini411 – 422ExtracellularSequence analysisAdd BLAST12
Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
Topological domaini444 – 500CytoplasmicSequence analysisAdd BLAST57

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Symptomatic deficiency in lactate transport (SDLT)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionDeficiency of lactate transporter may result in an acidic intracellular environment created by muscle activity with consequent degeneration of muscle and release of myoglobin and creatine kinase. This defect might compromise extreme performance in otherwise healthy individuals.
See also OMIM:245340
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_010434204K → E in SDLT. 1 PublicationCorresponds to variant dbSNP:rs80358222EnsemblClinVar.1
Natural variantiVAR_010435472G → R in SDLT. 1 PublicationCorresponds to variant dbSNP:rs72552271EnsemblClinVar.1
Familial hyperinsulinemic hypoglycemia 7 (HHF7)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionDominantly inherited hypoglycemic disorder characterized by inappropriate insulin secretion during anaerobic exercise or on pyruvate load.
See also OMIM:610021
Monocarboxylate transporter 1 deficiency (MCT1D)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA metabolic disorder characterized by recurrent ketoacidosis, a pathologic state due to ketone formation exceeding ketone utilization. The clinical consequences of ketoacidosis are vomiting, osmotic diuresis, dehydration, and Kussmaul breathing. The condition may progress to decreased consciousness and, ultimately, death.
See also OMIM:616095
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_072428313R → Q in MCT1D. 1 PublicationCorresponds to variant dbSNP:rs606231302EnsemblClinVar.1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi6566
MalaCardsiSLC16A1
MIMi245340 phenotype
610021 phenotype
616095 phenotype
OpenTargetsiENSG00000155380
Orphaneti165991 Exercise-induced hyperinsulinism
171690 Metabolic myopathy due to lactate transporter defect
PharmGKBiPA35813

Chemistry databases

ChEMBLiCHEMBL4360
DrugBankiDB04319 6-Deoxyglucose
DB03166 Acetic acid
DB01762 Acetoacetic Acid
DB04074 Alpha-ketoisovalerate
DB00345 Aminohippuric acid
DB00415 Ampicillin
DB08892 Arbaclofen Placarbil
DB03793 Benzoic Acid
DB03066 D-Lactic Acid
DB00529 Foscarnet
DB01440 Gamma Hydroxybutyric Acid
DB00142 L-Glutamic Acid
DB04398 Lactic Acid
DB00563 Methotrexate
DB00731 Nateglinide
DB00627 Niacin
DB04552 Niflumic Acid
DB00175 Pravastatin
DB01032 Probenecid
DB00119 Pyruvic acid
DB04216 Quercetin
DB00936 Salicylic acid
DB04348 Taurocholic Acid
DB00313 Valproic Acid
GuidetoPHARMACOLOGYi988

Polymorphism and mutation databases

BioMutaiSLC16A1
DMDMi313104214

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002113811 – 500Monocarboxylate transporter 1Add BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei210PhosphoserineBy similarity1
Modified residuei213PhosphoserineCombined sources1
Modified residuei231PhosphothreonineBy similarity1
Modified residuei461PhosphoserineCombined sources1
Modified residuei466PhosphothreonineCombined sources1
Modified residuei467PhosphoserineCombined sources1
Modified residuei483PhosphoserineCombined sources1
Modified residuei498PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP53985
MaxQBiP53985
PaxDbiP53985
PeptideAtlasiP53985
PRIDEiP53985
ProteomicsDBi56635

PTM databases

iPTMnetiP53985
PhosphoSitePlusiP53985
SwissPalmiP53985

Miscellaneous databases

PMAP-CutDBiP53985

Expressioni

Tissue specificityi

Detected in heart and in blood lymphocytes and monocytes (at protein level). Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000155380 Expressed in 237 organ(s), highest expression level in pigmented layer of retina
CleanExiHS_SLC16A1
ExpressionAtlasiP53985 baseline and differential
GenevisibleiP53985 HS

Organism-specific databases

HPAiCAB017489
CAB079035
HPA003324
HPA071055

Interactioni

Subunit structurei

Interacts with EMB. Interaction with either BSG or EMB is required for expression at the cell membrane (By similarity). Interacts with BSG; this is required for expression at the cell membrane.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112454, 29 interactors
IntActiP53985, 17 interactors
MINTiP53985
STRINGi9606.ENSP00000358640

Chemistry databases

BindingDBiP53985

Structurei

3D structure databases

ProteinModelPortaliP53985
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2504 Eukaryota
COG0477 LUCA
GeneTreeiENSGT00760000118926
HOGENOMiHOG000280688
HOVERGENiHBG006384
InParanoidiP53985
KOiK08179
OMAiRPFVGMF
OrthoDBiEOG091G05SW
PhylomeDBiP53985
TreeFamiTF313792

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR004743 MCT
IPR030757 MCT1
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
PANTHERiPTHR11360:SF24 PTHR11360:SF24, 1 hit
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 2 hits
TIGRFAMsiTIGR00892 2A0113, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: P53985-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPPAVGGPVG YTPPDGGWGW AVVIGAFISI GFSYAFPKSI TVFFKEIEGI
60 70 80 90 100
FHATTSEVSW ISSIMLAVMY GGGPISSILV NKYGSRIVMI VGGCLSGCGL
110 120 130 140 150
IAASFCNTVQ QLYVCIGVIG GLGLAFNLNP ALTMIGKYFY KRRPLANGLA
160 170 180 190 200
MAGSPVFLCT LAPLNQVFFG IFGWRGSFLI LGGLLLNCCV AGALMRPIGP
210 220 230 240 250
KPTKAGKDKS KASLEKAGKS GVKKDLHDAN TDLIGRHPKQ EKRSVFQTIN
260 270 280 290 300
QFLDLTLFTH RGFLLYLSGN VIMFFGLFAP LVFLSSYGKS QHYSSEKSAF
310 320 330 340 350
LLSILAFVDM VARPSMGLVA NTKPIRPRIQ YFFAASVVAN GVCHMLAPLS
360 370 380 390 400
TTYVGFCVYA GFFGFAFGWL SSVLFETLMD LVGPQRFSSA VGLVTIVECC
410 420 430 440 450
PVLLGPPLLG RLNDMYGDYK YTYWACGVVL IISGIYLFIG MGINYRLLAK
460 470 480 490 500
EQKANEQKKE SKEEETSIDV AGKPNEVTKA AESPDQKDTD GGPKEEESPV
Length:500
Mass (Da):53,944
Last modified:November 30, 2010 - v3
Checksum:i3F5B048CB962ECC8
GO
Isoform 2 (identifier: P53985-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     411-500: RLNDMYGDYK...GGPKEEESPV → IVYLPTNVGLLQNKHVRWEC

Note: No experimental confirmation available.
Show »
Length:430
Mass (Da):46,234
Checksum:i28C6B55692E6C4BE
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5T8R5Q5T8R5_HUMAN
Monocarboxylate transporter 1
SLC16A1 hCG_37455
456Annotation score:
Q5T8R3Q5T8R3_HUMAN
Monocarboxylate transporter 1
SLC16A1
296Annotation score:
Q5T8R4Q5T8R4_HUMAN
Monocarboxylate transporter 1
SLC16A1
215Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti480A → T in AAC41707 (PubMed:7835905).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05480485S → G. Corresponds to variant dbSNP:rs11551867Ensembl.1
Natural variantiVAR_010434204K → E in SDLT. 1 PublicationCorresponds to variant dbSNP:rs80358222EnsemblClinVar.1
Natural variantiVAR_072428313R → Q in MCT1D. 1 PublicationCorresponds to variant dbSNP:rs606231302EnsemblClinVar.1
Natural variantiVAR_010435472G → R in SDLT. 1 PublicationCorresponds to variant dbSNP:rs72552271EnsemblClinVar.1
Natural variantiVAR_010436490D → ECombined sources3 PublicationsCorresponds to variant dbSNP:rs1049434EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056191411 – 500RLNDM…EESPV → IVYLPTNVGLLQNKHVRWEC in isoform 2. 1 PublicationAdd BLAST90

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31801 mRNA Translation: AAC41707.1
AJ438945 Genomic DNA Translation: CAD27707.1
AL162079 mRNA Translation: CAB82412.1
AL158844 Genomic DNA No translation available.
CH471122 Genomic DNA Translation: EAW56552.1
BC026317 mRNA Translation: AAH26317.1
BC045664 mRNA Translation: AAH45664.1
CCDSiCCDS858.1 [P53985-1]
PIRiA55568
RefSeqiNP_001159968.1, NM_001166496.1 [P53985-1]
NP_003042.3, NM_003051.3 [P53985-1]
XP_011540328.1, XM_011542026.2 [P53985-1]
XP_011540329.1, XM_011542027.2 [P53985-1]
UniGeneiHs.75231

Genome annotation databases

EnsembliENST00000369626; ENSP00000358640; ENSG00000155380 [P53985-1]
ENST00000538576; ENSP00000441065; ENSG00000155380 [P53985-1]
ENST00000628110; ENSP00000485688; ENSG00000281917 [P53985-1]
ENST00000630362; ENSP00000486000; ENSG00000281917 [P53985-1]
GeneIDi6566
KEGGihsa:6566
UCSCiuc001ecx.4 human [P53985-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31801 mRNA Translation: AAC41707.1
AJ438945 Genomic DNA Translation: CAD27707.1
AL162079 mRNA Translation: CAB82412.1
AL158844 Genomic DNA No translation available.
CH471122 Genomic DNA Translation: EAW56552.1
BC026317 mRNA Translation: AAH26317.1
BC045664 mRNA Translation: AAH45664.1
CCDSiCCDS858.1 [P53985-1]
PIRiA55568
RefSeqiNP_001159968.1, NM_001166496.1 [P53985-1]
NP_003042.3, NM_003051.3 [P53985-1]
XP_011540328.1, XM_011542026.2 [P53985-1]
XP_011540329.1, XM_011542027.2 [P53985-1]
UniGeneiHs.75231

3D structure databases

ProteinModelPortaliP53985
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112454, 29 interactors
IntActiP53985, 17 interactors
MINTiP53985
STRINGi9606.ENSP00000358640

Chemistry databases

BindingDBiP53985
ChEMBLiCHEMBL4360
DrugBankiDB04319 6-Deoxyglucose
DB03166 Acetic acid
DB01762 Acetoacetic Acid
DB04074 Alpha-ketoisovalerate
DB00345 Aminohippuric acid
DB00415 Ampicillin
DB08892 Arbaclofen Placarbil
DB03793 Benzoic Acid
DB03066 D-Lactic Acid
DB00529 Foscarnet
DB01440 Gamma Hydroxybutyric Acid
DB00142 L-Glutamic Acid
DB04398 Lactic Acid
DB00563 Methotrexate
DB00731 Nateglinide
DB00627 Niacin
DB04552 Niflumic Acid
DB00175 Pravastatin
DB01032 Probenecid
DB00119 Pyruvic acid
DB04216 Quercetin
DB00936 Salicylic acid
DB04348 Taurocholic Acid
DB00313 Valproic Acid
GuidetoPHARMACOLOGYi988

Protein family/group databases

TCDBi2.A.1.13.1 the major facilitator superfamily (mfs)

PTM databases

iPTMnetiP53985
PhosphoSitePlusiP53985
SwissPalmiP53985

Polymorphism and mutation databases

BioMutaiSLC16A1
DMDMi313104214

Proteomic databases

EPDiP53985
MaxQBiP53985
PaxDbiP53985
PeptideAtlasiP53985
PRIDEiP53985
ProteomicsDBi56635

Protocols and materials databases

DNASUi6566
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369626; ENSP00000358640; ENSG00000155380 [P53985-1]
ENST00000538576; ENSP00000441065; ENSG00000155380 [P53985-1]
ENST00000628110; ENSP00000485688; ENSG00000281917 [P53985-1]
ENST00000630362; ENSP00000486000; ENSG00000281917 [P53985-1]
GeneIDi6566
KEGGihsa:6566
UCSCiuc001ecx.4 human [P53985-1]

Organism-specific databases

CTDi6566
DisGeNETi6566
EuPathDBiHostDB:ENSG00000155380.11
GeneCardsiSLC16A1
H-InvDBiHIX0000897
HGNCiHGNC:10922 SLC16A1
HPAiCAB017489
CAB079035
HPA003324
HPA071055
MalaCardsiSLC16A1
MIMi245340 phenotype
600682 gene
610021 phenotype
616095 phenotype
neXtProtiNX_P53985
OpenTargetsiENSG00000155380
Orphaneti165991 Exercise-induced hyperinsulinism
171690 Metabolic myopathy due to lactate transporter defect
PharmGKBiPA35813
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2504 Eukaryota
COG0477 LUCA
GeneTreeiENSGT00760000118926
HOGENOMiHOG000280688
HOVERGENiHBG006384
InParanoidiP53985
KOiK08179
OMAiRPFVGMF
OrthoDBiEOG091G05SW
PhylomeDBiP53985
TreeFamiTF313792

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000155380-MONOMER
ReactomeiR-HSA-210991 Basigin interactions
R-HSA-433692 Proton-coupled monocarboxylate transport
R-HSA-5619070 Defective SLC16A1 causes symptomatic deficiency in lactate transport (SDLT)
R-HSA-70268 Pyruvate metabolism
SABIO-RKiP53985

Miscellaneous databases

ChiTaRSiSLC16A1 human
GenomeRNAii6566
PMAP-CutDBiP53985
PROiPR:P53985
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000155380 Expressed in 237 organ(s), highest expression level in pigmented layer of retina
CleanExiHS_SLC16A1
ExpressionAtlasiP53985 baseline and differential
GenevisibleiP53985 HS

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR004743 MCT
IPR030757 MCT1
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
PANTHERiPTHR11360:SF24 PTHR11360:SF24, 1 hit
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 2 hits
TIGRFAMsiTIGR00892 2A0113, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMOT1_HUMAN
AccessioniPrimary (citable) accession number: P53985
Secondary accession number(s): Q49A45, Q5T8R6, Q9NSJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 30, 2010
Last modified: September 12, 2018
This is version 183 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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