UniProtKB - P53950 (VAC7_YEAST)
Vacuolar segregation protein 7
VAC7
Functioni
The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Positively regulates FAB1 kinase activity. Major activator of FAB1 during hyperosmotic shock and can elevate levels of PtdIns(3,5)P2 in the absence of VAC14 and FIG4. Directly involved in vacuolar membrane scission. Required for normal vacuole acidification, inheritance and morphology.
4 PublicationsGO - Biological processi
- 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate metabolic process Source: ComplexPortal
- positive regulation of kinase activity Source: SGD
- positive regulation of phosphatidylinositol biosynthetic process Source: SGD
- protein localization to vacuolar membrane Source: SGD
- regulation of phosphatidylinositol biosynthetic process Source: ComplexPortal
- vacuole inheritance Source: GO_Central
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar segregation protein 7 |
Gene namesi | Name:VAC7 Ordered Locus Names:YNL054W ORF Names:N2467, YNL2467W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004999, VAC7 |
VEuPathDBi | FungiDB:YNL054W |
Subcellular locationi
Vacuole
- Vacuole membrane 2 Publications; Single-pass type II membrane protein 2 Publications
Vacuole
- fungal-type vacuole membrane Source: SGD
- PAS complex Source: SGD
- vacuolar membrane Source: UniProtKB
Other locations
- cytoplasm Source: SGD
- integral component of membrane Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 919 | CytoplasmicSequence analysisAdd BLAST | 919 | |
Transmembranei | 920 – 940 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 941 – 1165 | VacuolarSequence analysisAdd BLAST | 225 |
Keywords - Cellular componenti
Membrane, VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000065757 | 1 – 1165 | Vacuolar segregation protein 7Add BLAST | 1165 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 164 | PhosphoserineCombined sources | 1 | |
Glycosylationi | 1020 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1099 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
MaxQBi | P53950 |
PaxDbi | P53950 |
PRIDEi | P53950 |
PTM databases
iPTMneti | P53950 |
Interactioni
Subunit structurei
Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold.
1 PublicationBinary interactionsi
P53950
With | #Exp. | IntAct |
---|---|---|
VAC14 [Q06708] | 4 | EBI-28714,EBI-27189 |
Protein-protein interaction databases
BioGRIDi | 35770, 387 interactors |
ComplexPortali | CPX-3088, PAS complex |
DIPi | DIP-4368N |
IntActi | P53950, 4 interactors |
MINTi | P53950 |
STRINGi | 4932.YNL054W |
Miscellaneous databases
RNActi | P53950, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 118 – 147 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 215 – 241 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 274 – 423 | DisorderedSequence analysisAdd BLAST | 150 | |
Regioni | 461 – 497 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 560 – 668 | DisorderedSequence analysisAdd BLAST | 109 | |
Regioni | 1074 – 1121 | DisorderedSequence analysisAdd BLAST | 48 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 215 – 231 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 275 – 289 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 306 – 331 | Basic and acidic residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 332 – 414 | Polar residuesSequence analysisAdd BLAST | 83 | |
Compositional biasi | 1084 – 1098 | Acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 1099 – 1121 | Basic and acidic residuesSequence analysisAdd BLAST | 23 |
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QU5B, Eukaryota |
HOGENOMi | CLU_006002_0_0_1 |
InParanoidi | P53950 |
OMAi | GNTISCP |
Family and domain databases
InterProi | View protein in InterPro IPR024260, Vac7 |
PANTHERi | PTHR28258, PTHR28258, 1 hit |
Pfami | View protein in Pfam PF12751, Vac7, 2 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTEEDRKLTV ETETVEAPVA NNLLLSNNSN VVAPNPSIPS ASTSTSPLHR
60 70 80 90 100
EIVDDSVATA NTTSNVVQHN LPTIDNNLMD SDATSHNQDH WHSDINRAGT
110 120 130 140 150
SMSTSDIPTD LHLEHIGSVS STNNNSNNAL INHNPLSSHL SNPSSSLRNK
160 170 180 190 200
KSSLLVASNP AFASDVELSK KKPAVISNNM PTSNIALYQT ARSANIHGPS
210 220 230 240 250
STSASKAFRK ASAFSNNTAP STSNNIGSNT PPAPLLPLPS LSQQNKPKII
260 270 280 290 300
ERPTMHVTNS REILLGENLL DDTKAKNAPA NSTTHDNGPV ANDGLRIPNH
310 320 330 340 350
SNADDNENNN KMKKNKNINS GKNERNDDTS KICTTSTKTA PSTAPLGSTD
360 370 380 390 400
NTQALTASVS SSNADNHNNN KKKTSSNNNG NNSNSASNKT NADIKNSNAD
410 420 430 440 450
LSASTSNNNA INDDSHESNS EKPTKADFFA ARLATAVGEN EISDSEETFV
460 470 480 490 500
YESAANSTKN LIFPDSSSQQ QQQQQQPPKQ QQQQQNHGIT SKISAPLLNN
510 520 530 540 550
NKKLLSRLKN SRHISTGAIL NNTIATISTN PNLNSNVMQN NNNLMSGHNH
560 570 580 590 600
LDELSSIKQE PPHQLQQQQP PMDVQSVDSY TSDNPDSNVI AKSPDKRSSL
610 620 630 640 650
VSLSKVSPHL LSSTSSNGNT ISCPNVATNS QELEPNNDIS TKKSLSNSTL
660 670 680 690 700
RHSSANRNSN YGDNKRPLRT TVSKIFDSNP NGAPLRRYSG VPDHVNLEDY
710 720 730 740 750
IEQPHNYPTM QNSVKKDEFY NSRNNKFPHG LNFYGDNNVI EEENNGDSSN
760 770 780 790 800
VNRPQHTNLQ HEFIPEDNES DENDIHSMFY YNHKNDLETK PLISDYGEDE
810 820 830 840 850
DVDDYDRPNA TFNSYYGSAS NTHELPLHGR MPSRSNNDYY DFMVGNNTGN
860 870 880 890 900
NNQLNEYTPL RMKRGQRHLS RTNNSIMNGS IHMNGNDDVT HSNINNNDIV
910 920 930 940 950
GYSPHNFYSR KSPFVKVKNF LYLAFVISSL LMTGFILGFL LATNKELQDV
960 970 980 990 1000
DVVVMDNVIS SSDELIFDIT VSAFNPGFFS ISVSQVDLDI FAKSSYLKCD
1010 1020 1030 1040 1050
SNGDCTVMEQ ERKILQITTN LSLVEESANN DISGGNIETV LLGTAKKLET
1060 1070 1080 1090 1100
PLKFQGGAFN RNYDVSVSSV KLLSPGSREA KHENDDDDDD DGDDGDDENN
1110 1120 1130 1140 1150
TNERQYKSKP NARDDKEDDT KKWKLLIKHD YELIVRGSMK YEVPFFNTQK
1160
STAIQKDSMV HPGKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U12141 Genomic DNA Translation: AAA99658.1 Z71330 Genomic DNA Translation: CAA95925.1 BK006947 Genomic DNA Translation: DAA10491.1 |
PIRi | S62982 |
RefSeqi | NP_014344.3, NM_001182893.3 |
Genome annotation databases
EnsemblFungii | YNL054W_mRNA; YNL054W; YNL054W |
GeneIDi | 855673 |
KEGGi | sce:YNL054W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U12141 Genomic DNA Translation: AAA99658.1 Z71330 Genomic DNA Translation: CAA95925.1 BK006947 Genomic DNA Translation: DAA10491.1 |
PIRi | S62982 |
RefSeqi | NP_014344.3, NM_001182893.3 |
3D structure databases
AlphaFoldDBi | P53950 |
SMRi | P53950 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35770, 387 interactors |
ComplexPortali | CPX-3088, PAS complex |
DIPi | DIP-4368N |
IntActi | P53950, 4 interactors |
MINTi | P53950 |
STRINGi | 4932.YNL054W |
PTM databases
iPTMneti | P53950 |
Proteomic databases
MaxQBi | P53950 |
PaxDbi | P53950 |
PRIDEi | P53950 |
Genome annotation databases
EnsemblFungii | YNL054W_mRNA; YNL054W; YNL054W |
GeneIDi | 855673 |
KEGGi | sce:YNL054W |
Organism-specific databases
SGDi | S000004999, VAC7 |
VEuPathDBi | FungiDB:YNL054W |
Phylogenomic databases
eggNOGi | ENOG502QU5B, Eukaryota |
HOGENOMi | CLU_006002_0_0_1 |
InParanoidi | P53950 |
OMAi | GNTISCP |
Miscellaneous databases
PROi | PR:P53950 |
RNActi | P53950, protein |
Family and domain databases
InterProi | View protein in InterPro IPR024260, Vac7 |
PANTHERi | PTHR28258, PTHR28258, 1 hit |
Pfami | View protein in Pfam PF12751, Vac7, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | VAC7_YEAST | |
Accessioni | P53950Primary (citable) accession number: P53950 Secondary accession number(s): D6W1C5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names