Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar segregation protein 7

Gene

VAC7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Positively regulates FAB1 kinase activity. Major activator of FAB1 during hyperosmotic shock and can elevate levels of PtdIns(3,5)P2 in the absence of VAC14 and FIG4. Directly involved in vacuolar membrane scission. Required for normal vacuole acidification, inheritance and morphology.4 Publications

GO - Biological processi

  • positive regulation of kinase activity Source: SGD
  • positive regulation of phosphatidylinositol biosynthetic process Source: SGD
  • protein localization to vacuolar membrane Source: SGD

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33086-MONOMER
YEAST:G3O-33086-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar segregation protein 7
Gene namesi
Name:VAC7
Ordered Locus Names:YNL054W
ORF Names:N2467, YNL2467W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

SGDiS000004999 VAC7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 919CytoplasmicSequence analysisAdd BLAST919
Transmembranei920 – 940Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini941 – 1165VacuolarSequence analysisAdd BLAST225

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000657571 – 1165Vacuolar segregation protein 7Add BLAST1165

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei164PhosphoserineCombined sources1
Glycosylationi1020N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1099N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP53950
PaxDbiP53950
PRIDEiP53950

PTM databases

iPTMnetiP53950

Interactioni

Subunit structurei

Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VAC14Q067084EBI-28714,EBI-27189

Protein-protein interaction databases

BioGridi35770, 384 interactors
ComplexPortaliCPX-3088 PAS complex
DIPiDIP-4368N
IntActiP53950, 4 interactors
MINTiP53950
STRINGi4932.YNL054W

Structurei

3D structure databases

ProteinModelPortaliP53950
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi277 – 412Asn-richAdd BLAST136
Compositional biasi469 – 485Gln-richAdd BLAST17
Compositional biasi1085 – 1119Asp-richAdd BLAST35

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiP53950
OMAiAFNPGWF
OrthoDBiEOG092C50ZN

Family and domain databases

InterProiView protein in InterPro
IPR024260 Vac7
PfamiView protein in Pfam
PF12751 Vac7, 2 hits

Sequencei

Sequence statusi: Complete.

P53950-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTEEDRKLTV ETETVEAPVA NNLLLSNNSN VVAPNPSIPS ASTSTSPLHR
60 70 80 90 100
EIVDDSVATA NTTSNVVQHN LPTIDNNLMD SDATSHNQDH WHSDINRAGT
110 120 130 140 150
SMSTSDIPTD LHLEHIGSVS STNNNSNNAL INHNPLSSHL SNPSSSLRNK
160 170 180 190 200
KSSLLVASNP AFASDVELSK KKPAVISNNM PTSNIALYQT ARSANIHGPS
210 220 230 240 250
STSASKAFRK ASAFSNNTAP STSNNIGSNT PPAPLLPLPS LSQQNKPKII
260 270 280 290 300
ERPTMHVTNS REILLGENLL DDTKAKNAPA NSTTHDNGPV ANDGLRIPNH
310 320 330 340 350
SNADDNENNN KMKKNKNINS GKNERNDDTS KICTTSTKTA PSTAPLGSTD
360 370 380 390 400
NTQALTASVS SSNADNHNNN KKKTSSNNNG NNSNSASNKT NADIKNSNAD
410 420 430 440 450
LSASTSNNNA INDDSHESNS EKPTKADFFA ARLATAVGEN EISDSEETFV
460 470 480 490 500
YESAANSTKN LIFPDSSSQQ QQQQQQPPKQ QQQQQNHGIT SKISAPLLNN
510 520 530 540 550
NKKLLSRLKN SRHISTGAIL NNTIATISTN PNLNSNVMQN NNNLMSGHNH
560 570 580 590 600
LDELSSIKQE PPHQLQQQQP PMDVQSVDSY TSDNPDSNVI AKSPDKRSSL
610 620 630 640 650
VSLSKVSPHL LSSTSSNGNT ISCPNVATNS QELEPNNDIS TKKSLSNSTL
660 670 680 690 700
RHSSANRNSN YGDNKRPLRT TVSKIFDSNP NGAPLRRYSG VPDHVNLEDY
710 720 730 740 750
IEQPHNYPTM QNSVKKDEFY NSRNNKFPHG LNFYGDNNVI EEENNGDSSN
760 770 780 790 800
VNRPQHTNLQ HEFIPEDNES DENDIHSMFY YNHKNDLETK PLISDYGEDE
810 820 830 840 850
DVDDYDRPNA TFNSYYGSAS NTHELPLHGR MPSRSNNDYY DFMVGNNTGN
860 870 880 890 900
NNQLNEYTPL RMKRGQRHLS RTNNSIMNGS IHMNGNDDVT HSNINNNDIV
910 920 930 940 950
GYSPHNFYSR KSPFVKVKNF LYLAFVISSL LMTGFILGFL LATNKELQDV
960 970 980 990 1000
DVVVMDNVIS SSDELIFDIT VSAFNPGFFS ISVSQVDLDI FAKSSYLKCD
1010 1020 1030 1040 1050
SNGDCTVMEQ ERKILQITTN LSLVEESANN DISGGNIETV LLGTAKKLET
1060 1070 1080 1090 1100
PLKFQGGAFN RNYDVSVSSV KLLSPGSREA KHENDDDDDD DGDDGDDENN
1110 1120 1130 1140 1150
TNERQYKSKP NARDDKEDDT KKWKLLIKHD YELIVRGSMK YEVPFFNTQK
1160
STAIQKDSMV HPGKK
Length:1,165
Mass (Da):128,140
Last modified:October 1, 1996 - v1
Checksum:i0414978AA3066CE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12141 Genomic DNA Translation: AAA99658.1
Z71330 Genomic DNA Translation: CAA95925.1
BK006947 Genomic DNA Translation: DAA10491.1
PIRiS62982
RefSeqiNP_014344.3, NM_001182893.3

Genome annotation databases

EnsemblFungiiYNL054W; YNL054W; YNL054W
GeneIDi855673
KEGGisce:YNL054W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12141 Genomic DNA Translation: AAA99658.1
Z71330 Genomic DNA Translation: CAA95925.1
BK006947 Genomic DNA Translation: DAA10491.1
PIRiS62982
RefSeqiNP_014344.3, NM_001182893.3

3D structure databases

ProteinModelPortaliP53950
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35770, 384 interactors
ComplexPortaliCPX-3088 PAS complex
DIPiDIP-4368N
IntActiP53950, 4 interactors
MINTiP53950
STRINGi4932.YNL054W

PTM databases

iPTMnetiP53950

Proteomic databases

MaxQBiP53950
PaxDbiP53950
PRIDEiP53950

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL054W; YNL054W; YNL054W
GeneIDi855673
KEGGisce:YNL054W

Organism-specific databases

SGDiS000004999 VAC7

Phylogenomic databases

InParanoidiP53950
OMAiAFNPGWF
OrthoDBiEOG092C50ZN

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33086-MONOMER
YEAST:G3O-33086-MONOMER

Miscellaneous databases

PROiPR:P53950

Family and domain databases

InterProiView protein in InterPro
IPR024260 Vac7
PfamiView protein in Pfam
PF12751 Vac7, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiVAC7_YEAST
AccessioniPrimary (citable) accession number: P53950
Secondary accession number(s): D6W1C5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again