UniProtKB - P53927 (NOP15_YEAST)
Protein
Ribosome biogenesis protein 15
Gene
NOP15
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Involved in the biogenesis of the 60S ribosomal subunit. Required for pre-rRNA processing and cytokinesis. Associates with the precursors of the 25S and 5.8S rRNAs.2 Publications
Miscellaneous
Present with 4280 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- RNA binding Source: GO_Central
- rRNA binding Source: GO_Central
GO - Biological processi
- maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
- ribosomal large subunit biogenesis Source: SGD
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Biological process | Ribosome biogenesis, rRNA processing |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosome biogenesis protein 15Alternative name(s): Nucleolar protein 151 Publication |
Gene namesi | Name:NOP151 Publication Ordered Locus Names:YNL110C ORF Names:N1954 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005054, NOP15 |
VEuPathDBi | FungiDB:YNL110C |
Subcellular locationi
Nucleus
Other locations
- cytoplasm Source: UniProtKB-SubCell
- preribosome, large subunit precursor Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Essential gene. Abrupt growth arrest prior to substantial depletion of ribosomal subunits. Fails to synthesize the 25S and 5.8S rRNA components of the 60S ribosomal subunit, and exonucleolytic 5' processing of 5.8S rRNA is strongly inhibited. Arrests at cytokinesis and fails to assemble a contractile actin ring at the bud neck.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082035 | 1 – 220 | Ribosome biogenesis protein 15Add BLAST | 220 |
Proteomic databases
MaxQBi | P53927 |
PaxDbi | P53927 |
PRIDEi | P53927 |
PTM databases
iPTMneti | P53927 |
Interactioni
Subunit structurei
Component of the pre-66S ribosomal particle.
Interacts with NOP7 and RRP1.
3 PublicationsBinary interactionsi
Hide detailsP53927
With | #Exp. | IntAct |
---|---|---|
CIC1 [P38779] | 3 | EBI-28853,EBI-24538 |
HAS1 [Q03532] | 4 | EBI-28853,EBI-8170 |
NSA2 [P40078] | 3 | EBI-28853,EBI-22681 |
PRP43 [P53131] | 3 | EBI-28853,EBI-505 |
Protein-protein interaction databases
BioGRIDi | 35715, 239 interactors |
DIPi | DIP-4741N |
IntActi | P53927, 54 interactors |
MINTi | P53927 |
STRINGi | 4932.YNL110C |
Miscellaneous databases
RNActi | P53927, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SASBDBi | P53927 |
SMRi | P53927 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 91 – 169 | RRMPROSITE-ProRule annotationAdd BLAST | 79 |
Phylogenomic databases
eggNOGi | KOG4208, Eukaryota |
GeneTreei | ENSGT00940000172371 |
HOGENOMi | CLU_025741_2_0_1 |
InParanoidi | P53927 |
OMAi | QETMNNY |
Family and domain databases
CDDi | cd12552, RRM_Nop15p, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR034469, Nop15_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
i Sequence
Sequence statusi: Complete.
P53927-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVKSTSKTST KETVTKQPTE EKPIQEKEEL ALETSSSSSD EEDEKDEDEI
60 70 80 90 100
EGLAASDDEQ SGTHKIKRLN PKKQANEKKS KDKKTLEEYS GIIYVSRLPH
110 120 130 140 150
GFHEKELSKY FAQFGDLKEV RLARNKKTGN SRHYGFLEFV NKEDAMIAQE
160 170 180 190 200
SMNNYLLMGH LLQVRVLPKG AKIEKLYKYK KRVLVEKGIT KPVKQLKDNM
210 220
KQKHEERIKK LAKSGIEFKW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z69382 Genomic DNA Translation: CAA93397.1 Z71386 Genomic DNA Translation: CAA95989.1 AY693098 Genomic DNA Translation: AAT93117.1 BK006947 Genomic DNA Translation: DAA10437.1 |
PIRi | S63051 |
RefSeqi | NP_014289.1, NM_001182948.1 |
Genome annotation databases
EnsemblFungii | YNL110C_mRNA; YNL110C; YNL110C |
GeneIDi | 855613 |
KEGGi | sce:YNL110C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z69382 Genomic DNA Translation: CAA93397.1 Z71386 Genomic DNA Translation: CAA95989.1 AY693098 Genomic DNA Translation: AAT93117.1 BK006947 Genomic DNA Translation: DAA10437.1 |
PIRi | S63051 |
RefSeqi | NP_014289.1, NM_001182948.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3JCT | electron microscopy | 3.08 | o | 1-220 | [»] | |
5T9P | X-ray | 2.00 | A/B/C/D | 81-191 | [»] | |
5Z3G | electron microscopy | 3.65 | E | 1-220 | [»] | |
6C0F | electron microscopy | 3.70 | o | 1-220 | [»] | |
6CB1 | electron microscopy | 4.60 | o | 1-220 | [»] | |
6ELZ | electron microscopy | 3.30 | o | 1-220 | [»] | |
6EM1 | electron microscopy | 3.60 | o | 1-220 | [»] | |
6EM3 | electron microscopy | 3.20 | o | 1-220 | [»] | |
6EM4 | electron microscopy | 4.10 | o | 1-220 | [»] | |
6EM5 | electron microscopy | 4.30 | o | 1-220 | [»] | |
6M62 | electron microscopy | 3.20 | o | 1-220 | [»] | |
6YLX | electron microscopy | 3.90 | o | 1-220 | [»] | |
6YLY | electron microscopy | 3.80 | o | 1-220 | [»] | |
7BTB | electron microscopy | 3.22 | o | 1-220 | [»] | |
SASBDBi | P53927 | |||||
SMRi | P53927 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35715, 239 interactors |
DIPi | DIP-4741N |
IntActi | P53927, 54 interactors |
MINTi | P53927 |
STRINGi | 4932.YNL110C |
PTM databases
iPTMneti | P53927 |
Proteomic databases
MaxQBi | P53927 |
PaxDbi | P53927 |
PRIDEi | P53927 |
Genome annotation databases
EnsemblFungii | YNL110C_mRNA; YNL110C; YNL110C |
GeneIDi | 855613 |
KEGGi | sce:YNL110C |
Organism-specific databases
SGDi | S000005054, NOP15 |
VEuPathDBi | FungiDB:YNL110C |
Phylogenomic databases
eggNOGi | KOG4208, Eukaryota |
GeneTreei | ENSGT00940000172371 |
HOGENOMi | CLU_025741_2_0_1 |
InParanoidi | P53927 |
OMAi | QETMNNY |
Miscellaneous databases
PROi | PR:P53927 |
RNActi | P53927, protein |
Family and domain databases
CDDi | cd12552, RRM_Nop15p, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR034469, Nop15_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NOP15_YEAST | |
Accessioni | P53927Primary (citable) accession number: P53927 Secondary accession number(s): D6W171 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references