UniProtKB - P53847 (DSL1_YEAST)
Protein transport protein DSL1
DSL1
Functioni
Required for protein transport between the Golgi and the endoplasmic reticulum. May tether coatomer-coated retrograde transport vesicles to the ER membrane through interaction with coatomer as well as the SNARE complex. May contribute to the stabilization of the SNARE complex.
3 PublicationsMiscellaneous
GO - Biological processi
- endoplasmic reticulum to Golgi vesicle-mediated transport Source: GO_Central
- ER-dependent peroxisome organization Source: SGD
- mitotic spindle assembly checkpoint signaling Source: GO_Central
- protein transport Source: UniProtKB-KW
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: ComplexPortal
Keywordsi
Biological process | ER-Golgi transport, Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-SCE-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Names & Taxonomyi
Protein namesi | Recommended name: Protein transport protein DSL1Alternative name(s): Dependent on SLY1-20 protein 1 |
Gene namesi | Name:DSL1 Ordered Locus Names:YNL258C ORF Names:N0842 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005202, DSL1 |
VEuPathDBi | FungiDB:YNL258C |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Peripheral membrane protein 2 Publications
Cytosol
- cytosol Source: SGD
Endoplasmic reticulum
- Dsl1/NZR complex Source: SGD
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Nucleus
- nucleus Source: SGD
Other locations
- RZZ complex Source: GO_Central
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 413 | W → A: Viable and reduced interaction with RET2, strong Golgi-ER retrograde transport defect. Loss of interaction with RET2; when associated with A-455. Lethal and loss of interactions with RET1 and RET2; when associated with A-425. 1 Publication | 1 | |
Mutagenesisi | 425 | W → A: Loss of interaction with RET1; when associated with A-413. 1 Publication | 1 | |
Mutagenesisi | 455 | W → A: Viable and no effect. Lethal and loss of interaction with RET2 and reduced interaction with RET1; when associated with A-413. 1 Publication | 1 | |
Mutagenesisi | 725 – 754 | Missing in dsl1-22; strong Golgi-ER retrograde transport defect. 1 PublicationAdd BLAST | 30 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000203383 | 1 – 754 | Protein transport protein DSL1Add BLAST | 754 |
Proteomic databases
MaxQBi | P53847 |
PaxDbi | P53847 |
PRIDEi | P53847 |
PTM databases
iPTMneti | P53847 |
Interactioni
Subunit structurei
Component of a peripheral membrane protein complex consisting of DSL1, SEC39/DSL3 and TIP20. Bound to a SNARE complex consisting of UFE1, USE1, SEC20 and SEC22 or YKT6 through direct interaction of TIP20 with SEC20. Binds the coatomer complex through direct interaction with RET2/COPD and RET1/COPA. Binds TIP20 and SEC39/DSL3.
Binary interactionsi
P53847
With | #Exp. | IntAct |
---|---|---|
SEC39 [Q12745] | 14 | EBI-29249,EBI-31898 |
TIP20 [P33891] | 14 | EBI-29249,EBI-19396 |
Protein-protein interaction databases
BioGRIDi | 35581, 75 interactors |
ComplexPortali | CPX-1786, Dsl1 tethering complex |
DIPi | DIP-4274N |
IntActi | P53847, 9 interactors |
STRINGi | 4932.YNL258C |
Miscellaneous databases
RNActi | P53847, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P53847 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P53847 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 200 | Interaction with TIP201 PublicationAdd BLAST | 200 | |
Regioni | 406 – 459 | Interaction with RET2Add BLAST | 54 | |
Regioni | 406 – 440 | Interaction with RET11 PublicationAdd BLAST | 35 | |
Regioni | 425 – 454 | DisorderedSequence analysisAdd BLAST | 30 |
Phylogenomic databases
eggNOGi | ENOG502QR7Q, Eukaryota |
HOGENOMi | CLU_357169_0_0_1 |
InParanoidi | P53847 |
OMAi | DFYLFST |
Family and domain databases
Gene3Di | 1.20.58.2230, 1 hit |
InterProi | View protein in InterPro IPR021876, Dsl1_C IPR021875, Dsl1_N_dom IPR038442, Dsl1_N_sf |
Pfami | View protein in Pfam PF11989, Dsl1_C, 1 hit PF11988, Dsl1_N, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MESLFPNKGE IIRELLKDPL ILKNDSKRSN GSELELDSSD LLQREAILAN
60 70 80 90 100
ELNILDNLKT FLNLIKEVKT NLNILELENC YYSLQSLRKK MRNNAAYLKQ
110 120 130 140 150
SFNFQQSIST YVDTLHLELV STLYKILTNG FWKITENSIQ FTPTVEWGKD
160 170 180 190 200
KVHIEYDTFM DFVAQQYFPK GSLDNQAWFI LDMTSADSQE QVRAKLNTIM
210 220 230 240 250
KEYMNLSRIV SMIKNSIFIS GKEISYENEK NILVFSKSSS HGQHCVSTVL
260 270 280 290 300
TSFEAVCDFM LDGLAFRDRK TLSYELGPLF NTEFTKFVKN NASIILESLD
310 320 330 340 350
SPLKNLVSVI NNKLTRLVAK SEVTNWTHSG KEIQDLLMNK QLYYNLLLDK
360 370 380 390 400
VLESHISEIR SIFEDPKKSW QNLEVVELTT SNTNTMSEKI GKNDSDVQNE
410 420 430 440 450
KELHNAVSKD DDWNWEVEDD DADAWGDEID VNIDDEEEKT NQEKEKEPEE
460 470 480 490 500
EENAWDEAWA IDENIDDASL ENGKEHLKAH DVGSLDKDHI EVTQLPKLFL
510 520 530 540 550
AISQNFKSSF ADSHVDEQYF AYKYNLLQTS YMAMCTANFS HNWCQLYVDM
560 570 580 590 600
RYLIERDEKL YRIKELTRNL LETKLNMKYR IVCQLIRHQL TEFRENERNP
610 620 630 640 650
SWDATIEKLL PYILKEIVRP LQKIRGEEGS RYLLSFLNFL YNDCVTKEIL
660 670 680 690 700
KWQIISEVNS ENLGELVSLL VNNTDIQLLA KEPSYKKMRE KFATMGKFLP
710 720 730 740 750
LHLKEIMEMF YNGDFYLFAT DELIQWIELL FADTPLRRNA IDDIYEIRGT
ALDD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X96722 Genomic DNA Translation: CAA65486.1 Z71534 Genomic DNA Translation: CAA96165.1 BK006947 Genomic DNA Translation: DAA10301.1 |
PIRi | S63231 |
RefSeqi | NP_014141.1, NM_001183096.1 |
Genome annotation databases
EnsemblFungii | YNL258C_mRNA; YNL258C; YNL258C |
GeneIDi | 855463 |
KEGGi | sce:YNL258C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X96722 Genomic DNA Translation: CAA65486.1 Z71534 Genomic DNA Translation: CAA96165.1 BK006947 Genomic DNA Translation: DAA10301.1 |
PIRi | S63231 |
RefSeqi | NP_014141.1, NM_001183096.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3ETU | X-ray | 2.40 | A | 1-361 | [»] | |
3ETV | X-ray | 1.94 | A | 37-339 | [»] | |
5FJW | X-ray | 2.80 | L/M/N/O/P/Q/R/S | 411-419 | [»] | |
5FJZ | X-ray | 1.90 | P/Q/R/S | 411-419 | [»] | |
SMRi | P53847 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35581, 75 interactors |
ComplexPortali | CPX-1786, Dsl1 tethering complex |
DIPi | DIP-4274N |
IntActi | P53847, 9 interactors |
STRINGi | 4932.YNL258C |
PTM databases
iPTMneti | P53847 |
Proteomic databases
MaxQBi | P53847 |
PaxDbi | P53847 |
PRIDEi | P53847 |
Genome annotation databases
EnsemblFungii | YNL258C_mRNA; YNL258C; YNL258C |
GeneIDi | 855463 |
KEGGi | sce:YNL258C |
Organism-specific databases
SGDi | S000005202, DSL1 |
VEuPathDBi | FungiDB:YNL258C |
Phylogenomic databases
eggNOGi | ENOG502QR7Q, Eukaryota |
HOGENOMi | CLU_357169_0_0_1 |
InParanoidi | P53847 |
OMAi | DFYLFST |
Enzyme and pathway databases
Reactomei | R-SCE-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Miscellaneous databases
EvolutionaryTracei | P53847 |
PROi | PR:P53847 |
RNActi | P53847, protein |
Family and domain databases
Gene3Di | 1.20.58.2230, 1 hit |
InterProi | View protein in InterPro IPR021876, Dsl1_C IPR021875, Dsl1_N_dom IPR038442, Dsl1_N_sf |
Pfami | View protein in Pfam PF11989, Dsl1_C, 1 hit PF11988, Dsl1_N, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DSL1_YEAST | |
Accessioni | P53847Primary (citable) accession number: P53847 Secondary accession number(s): D6W0T5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references