Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
We will be switching to the new UniProt website soon. Please explore and share your feedback. Take me to the new website.

UniProtKB - P53840 (TOF1_YEAST)

Entry version 167 (23 Feb 2022)
Sequence version 1 (01 Oct 1996)
Previous versions | rss
Add a publicationFeedback
Protein

Topoisomerase 1-associated factor 1

Gene

TOF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Forms a fork protection complex (FPC) with CSM3 and which is required for chromosome segregation during meiosis and DNA damage repair. FPC coordinates leading and lagging strand synthesis and moves with the replication fork. FPC stabilizes replication forks in a configuration that is recognized by replication checkpoint sensors and protects stalled replication forks against the fork-releasing activity of RRM3 helicase.

9 Publications

Miscellaneous

Present with 952 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • DNA binding Source: GO_Central
Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA replication inhibitor
Biological processCell cycle, DNA damage, DNA repair, Meiosis

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Topoisomerase 1-associated factor 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:TOF1
Ordered Locus Names:YNL273W
ORF Names:N0636
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XIV

Organism-specific databases

Saccharomyces Genome Database

More...SGDi		S000005217, TOF1

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		FungiDB:YNL273W

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000726201 – 1238Topoisomerase 1-associated factor 1Add BLAST1238

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei626PhosphoserineCombined sources1
Modified residuei654PhosphoserineCombined sources1
Modified residuei1056PhosphoserineCombined sources1
Modified residuei1058PhosphoserineCombined sources1
Modified residuei1213PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...MaxQBi		P53840

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P53840

PRoteomics IDEntifications database

More...PRIDEi		P53840

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P53840

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the fork protection complex (FPC) consisting of TOF1 and CSM3.

Interacts with WSS1 and ESC4.

4 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		35567, 320 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-1673, Replication fork protection complex

Database of interacting proteins

More...DIPi		DIP-4272N

Protein interaction database and analysis system

More...IntActi		P53840, 8 interactors

Molecular INTeraction database

More...MINTi		P53840

STRING: functional protein association networks

More...STRINGi		4932.YNL273W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P53840, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P53840

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1008 – 1051DisorderedSequence analysisAdd BLAST44
Regioni1159 – 1218DisorderedSequence analysisAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi1008 – 1022Basic residuesSequence analysisAdd BLAST15
Compositional biasi1023 – 1051Basic and acidic residuesSequence analysisAdd BLAST29
Compositional biasi1159 – 1195Polar residuesSequence analysisAdd BLAST37

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the timeless family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1974, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00390000015124

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_008440_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P53840

Identification of Orthologs from Complete Genome Data

More...OMAi		MTVNHHR

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR044998, Timeless
IPR006906, Timeless_N

The PANTHER Classification System

More...PANTHERi		PTHR22940, PTHR22940, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF04821, TIMELESS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P53840-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSADLQQGTT NAADFSLTVL RARIALLATA IGGPDYTSQI DPPPYKLGDD 
        60         70         80         90        100
CLACLKDLKR WFKLVDDQQK RWDVAMAVAE YRILTDDLLP ILIDWENKCS 
       110        120        130        140        150
LAAKLAKNNP DHEEFRNKAY YDKIALNCLQ LLVLMTWPLI VTEQSSSNQI 
       160        170        180        190        200
TLYGELKKHQ LVYKKTILSM ESGKVLRAAI RLALDVIKID RLSRTPRDNM 
       210        220        230        240        250
VLKLVLNFFR NVIAIEPGEF TINTKKSMPK KGITSIDTLP PNVSMDDISL 
       260        270        280        290        300
NTVISSFHKN KVFGFLLTLT SSLSKEFDQD FINIPLLEIM FYFTKDVNQE 
       310        320        330        340        350
LLFPRQFETG THSKVVNKNE SSSANNIVTS AGFELSKLLQ KEHQMRKNVI 
       360        370        380        390        400
KHTSARHSRF GGLLSIQTPD KTRLTVSGSQ ALVDEKIALQ KLDDSKKWNK 
       410        420        430        440        450
RIIKKHQSVA AEGLPNSLLN SQTGKAIFFT ESNGKHFKEF INNFIDSGFN 
       460        470        480        490        500
ILLHSVTNYF TTEQDRMVTL EQVEYLLFFA WFVKYQLLRS KIDNSADIKQ 
       510        520        530        540        550
VSEALKEVTF ILVSSLLRSA YDLKNWTVTH AGMIAFNELL NLVSRTKAAQ 
       560        570        580        590        600
EEDSTDIEFI VSRLFSDERI QLLSNLPKIG SKYSLQFMKS CIELTHSVLK 
       610        620        630        640        650
VLEQYSDDKT LVIEGKSRRQ KKFNISEGDI TKLIEEENVD RDEALDILTS 
       660        670        680        690        700
SLRSIEVNFQ KVQANYMTEP VIETYINFLE RFRELEDDSI KKVFSFFHRV 
       710        720        730        740        750
FVQAKEQALL FRFDLIILLR EMLSPDGLDR MSRSRKYVSQ FSDYFLARLK 
       760        770        780        790        800
KRLKKSPAWF VGLLFPPLHN SEVGFYQRYG EYNVLNNESM YAAPASQFKP 
       810        820        830        840        850
IPDEEALPPS ILLDMKYGVL VSTLLDDGKT ELLDQLLKHI THTLDIFKSW 
       860        870        880        890        900
LTVNVNAGKE TVNPPNEYFT LTGVLNNDPI FKDKDYRALL LLIGYSIPRK 
       910        920        930        940        950
INEPCFLPGT VEVSDLTVSC ELVKKYLSTP FETPNGLPSS SYLLRVRSEK 
       960        970        980        990       1000
DSFSHNEQDG WEGDDDYDYN DPYIVPDDQI LSKSDAAYFK DLDNNASDKL 
      1010       1020       1030       1040       1050
KGTKFSKGIA RSKKKDKRKR RKGEAKTNLP MFGDQDDERP QTVRERHGVF 
      1060       1070       1080       1090       1100
SKEFISDSED DEDLMNPIFF ENETYMRWLL DKNNGQLTED RYIQFAKFAA 
      1110       1120       1130       1140       1150
ERMNNGGVVT GDYTSLFGGS IPSIESIRAT ESSSFAPDKS LISLASHVAS 
      1160       1170       1180       1190       1200
EMSIFDVNNN NNNQLSDDDV NSESRNSLGS SQPSNSQNMF QSEVYSRKES 
      1210       1220       1230 
TKRSLEASAA DESDEDEEAI RLFGKKSRVV LSQGDSDD
Length:1,238
Mass (Da):141,120
Last modified:October 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iCF8FEC33EE0088E9
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
Z71549 Genomic DNA Translation: CAA96181.1
BK006947 Genomic DNA Translation: DAA10287.1

Protein sequence database of the Protein Information Resource

More...PIRi		S63247

NCBI Reference Sequences

More...RefSeqi		NP_014126.1, NM_001183111.1

Genome annotation databases

Ensembl fungal genome annotation project

More...EnsemblFungii		YNL273W_mRNA; YNL273W; YNL273W

Database of genes from NCBI RefSeq genomes

More...GeneIDi		855448

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		sce:YNL273W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71549 Genomic DNA Translation: CAA96181.1
BK006947 Genomic DNA Translation: DAA10287.1
PIRiS63247
RefSeqiNP_014126.1, NM_001183111.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6SKLelectron microscopy3.70X1-1238[»]
SMRiP53840
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi35567, 320 interactors
ComplexPortaliCPX-1673, Replication fork protection complex
DIPiDIP-4272N
IntActiP53840, 8 interactors
MINTiP53840
STRINGi4932.YNL273W

PTM databases

iPTMnetiP53840

Proteomic databases

MaxQBiP53840
PaxDbiP53840
PRIDEiP53840

Genome annotation databases

EnsemblFungiiYNL273W_mRNA; YNL273W; YNL273W
GeneIDi855448
KEGGisce:YNL273W

Organism-specific databases

SGDiS000005217, TOF1
VEuPathDBiFungiDB:YNL273W

Phylogenomic databases

eggNOGiKOG1974, Eukaryota
GeneTreeiENSGT00390000015124
HOGENOMiCLU_008440_0_0_1
InParanoidiP53840
OMAiMTVNHHR

Miscellaneous databases

Protein Ontology

More...PROi		PR:P53840
RNActiP53840, protein

Family and domain databases

InterProiView protein in InterPro
IPR044998, Timeless
IPR006906, Timeless_N
PANTHERiPTHR22940, PTHR22940, 1 hit
PfamiView protein in Pfam
PF04821, TIMELESS, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTOF1_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P53840
Secondary accession number(s): D6W0S1
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 23, 2022
This is version 167 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again