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UniProtKB - P53840 (TOF1_YEAST)
Protein
Topoisomerase 1-associated factor 1
Gene
TOF1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Forms a fork protection complex (FPC) with CSM3 and which is required for chromosome segregation during meiosis and DNA damage repair. FPC coordinates leading and lagging strand synthesis and moves with the replication fork. FPC stabilizes replication forks in a configuration that is recognized by replication checkpoint sensors and protects stalled replication forks against the fork-releasing activity of RRM3 helicase.
9 PublicationsMiscellaneous
Present with 952 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- DNA binding Source: GO_Central
GO - Biological processi
- DNA repair Source: ComplexPortal
- DNA replication Source: SGD
- DNA replication checkpoint signaling Source: SGD
- maintenance of DNA repeat elements Source: SGD
- meiotic cell cycle Source: UniProtKB-KW
- mitotic sister chromatid cohesion Source: SGD
- replication fork arrest Source: SGD
- replication fork protection Source: ComplexPortal
Keywordsi
Molecular function | DNA replication inhibitor |
Biological process | Cell cycle, DNA damage, DNA repair, Meiosis |
Names & Taxonomyi
Protein namesi | Recommended name: Topoisomerase 1-associated factor 1 |
Gene namesi | Name:TOF1 Ordered Locus Names:YNL273W ORF Names:N0636 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005217, TOF1 |
VEuPathDBi | FungiDB:YNL273W |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Note: Associated with chromatin during S phase.
Cytosol
- cytosol Source: SGD
Nucleus
- nuclear replication fork Source: SGD
- nucleus Source: SGD
- replication fork protection complex Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000072620 | 1 – 1238 | Topoisomerase 1-associated factor 1Add BLAST | 1238 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 626 | PhosphoserineCombined sources | 1 | |
Modified residuei | 654 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1056 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1058 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1213 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P53840 |
PaxDbi | P53840 |
PRIDEi | P53840 |
PTM databases
iPTMneti | P53840 |
Interactioni
Subunit structurei
Component of the fork protection complex (FPC) consisting of TOF1 and CSM3.
Interacts with WSS1 and ESC4.
4 PublicationsBinary interactionsi
P53840
With | #Exp. | IntAct |
---|---|---|
CDC45 [Q08032] | 3 | EBI-28257,EBI-4292 |
CSM3 [Q04659] | 7 | EBI-28257,EBI-28093 |
MRC1 [P25588] | 4 | EBI-28257,EBI-412442 |
Protein-protein interaction databases
BioGRIDi | 35567, 320 interactors |
ComplexPortali | CPX-1673, Replication fork protection complex |
DIPi | DIP-4272N |
IntActi | P53840, 8 interactors |
MINTi | P53840 |
STRINGi | 4932.YNL273W |
Miscellaneous databases
RNActi | P53840, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1008 – 1051 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 1159 – 1218 | DisorderedSequence analysisAdd BLAST | 60 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1008 – 1022 | Basic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 1023 – 1051 | Basic and acidic residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 1159 – 1195 | Polar residuesSequence analysisAdd BLAST | 37 |
Sequence similaritiesi
Belongs to the timeless family.Curated
Phylogenomic databases
eggNOGi | KOG1974, Eukaryota |
GeneTreei | ENSGT00390000015124 |
HOGENOMi | CLU_008440_0_0_1 |
InParanoidi | P53840 |
OMAi | MTVNHHR |
Family and domain databases
InterProi | View protein in InterPro IPR044998, Timeless IPR006906, Timeless_N |
PANTHERi | PTHR22940, PTHR22940, 1 hit |
Pfami | View protein in Pfam PF04821, TIMELESS, 1 hit |
i Sequence
Sequence statusi: Complete.
P53840-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSADLQQGTT NAADFSLTVL RARIALLATA IGGPDYTSQI DPPPYKLGDD
60 70 80 90 100
CLACLKDLKR WFKLVDDQQK RWDVAMAVAE YRILTDDLLP ILIDWENKCS
110 120 130 140 150
LAAKLAKNNP DHEEFRNKAY YDKIALNCLQ LLVLMTWPLI VTEQSSSNQI
160 170 180 190 200
TLYGELKKHQ LVYKKTILSM ESGKVLRAAI RLALDVIKID RLSRTPRDNM
210 220 230 240 250
VLKLVLNFFR NVIAIEPGEF TINTKKSMPK KGITSIDTLP PNVSMDDISL
260 270 280 290 300
NTVISSFHKN KVFGFLLTLT SSLSKEFDQD FINIPLLEIM FYFTKDVNQE
310 320 330 340 350
LLFPRQFETG THSKVVNKNE SSSANNIVTS AGFELSKLLQ KEHQMRKNVI
360 370 380 390 400
KHTSARHSRF GGLLSIQTPD KTRLTVSGSQ ALVDEKIALQ KLDDSKKWNK
410 420 430 440 450
RIIKKHQSVA AEGLPNSLLN SQTGKAIFFT ESNGKHFKEF INNFIDSGFN
460 470 480 490 500
ILLHSVTNYF TTEQDRMVTL EQVEYLLFFA WFVKYQLLRS KIDNSADIKQ
510 520 530 540 550
VSEALKEVTF ILVSSLLRSA YDLKNWTVTH AGMIAFNELL NLVSRTKAAQ
560 570 580 590 600
EEDSTDIEFI VSRLFSDERI QLLSNLPKIG SKYSLQFMKS CIELTHSVLK
610 620 630 640 650
VLEQYSDDKT LVIEGKSRRQ KKFNISEGDI TKLIEEENVD RDEALDILTS
660 670 680 690 700
SLRSIEVNFQ KVQANYMTEP VIETYINFLE RFRELEDDSI KKVFSFFHRV
710 720 730 740 750
FVQAKEQALL FRFDLIILLR EMLSPDGLDR MSRSRKYVSQ FSDYFLARLK
760 770 780 790 800
KRLKKSPAWF VGLLFPPLHN SEVGFYQRYG EYNVLNNESM YAAPASQFKP
810 820 830 840 850
IPDEEALPPS ILLDMKYGVL VSTLLDDGKT ELLDQLLKHI THTLDIFKSW
860 870 880 890 900
LTVNVNAGKE TVNPPNEYFT LTGVLNNDPI FKDKDYRALL LLIGYSIPRK
910 920 930 940 950
INEPCFLPGT VEVSDLTVSC ELVKKYLSTP FETPNGLPSS SYLLRVRSEK
960 970 980 990 1000
DSFSHNEQDG WEGDDDYDYN DPYIVPDDQI LSKSDAAYFK DLDNNASDKL
1010 1020 1030 1040 1050
KGTKFSKGIA RSKKKDKRKR RKGEAKTNLP MFGDQDDERP QTVRERHGVF
1060 1070 1080 1090 1100
SKEFISDSED DEDLMNPIFF ENETYMRWLL DKNNGQLTED RYIQFAKFAA
1110 1120 1130 1140 1150
ERMNNGGVVT GDYTSLFGGS IPSIESIRAT ESSSFAPDKS LISLASHVAS
1160 1170 1180 1190 1200
EMSIFDVNNN NNNQLSDDDV NSESRNSLGS SQPSNSQNMF QSEVYSRKES
1210 1220 1230
TKRSLEASAA DESDEDEEAI RLFGKKSRVV LSQGDSDD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z71549 Genomic DNA Translation: CAA96181.1 BK006947 Genomic DNA Translation: DAA10287.1 |
PIRi | S63247 |
RefSeqi | NP_014126.1, NM_001183111.1 |
Genome annotation databases
EnsemblFungii | YNL273W_mRNA; YNL273W; YNL273W |
GeneIDi | 855448 |
KEGGi | sce:YNL273W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z71549 Genomic DNA Translation: CAA96181.1 BK006947 Genomic DNA Translation: DAA10287.1 |
PIRi | S63247 |
RefSeqi | NP_014126.1, NM_001183111.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6SKL | electron microscopy | 3.70 | X | 1-1238 | [»] | |
SMRi | P53840 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35567, 320 interactors |
ComplexPortali | CPX-1673, Replication fork protection complex |
DIPi | DIP-4272N |
IntActi | P53840, 8 interactors |
MINTi | P53840 |
STRINGi | 4932.YNL273W |
PTM databases
iPTMneti | P53840 |
Proteomic databases
MaxQBi | P53840 |
PaxDbi | P53840 |
PRIDEi | P53840 |
Genome annotation databases
EnsemblFungii | YNL273W_mRNA; YNL273W; YNL273W |
GeneIDi | 855448 |
KEGGi | sce:YNL273W |
Organism-specific databases
SGDi | S000005217, TOF1 |
VEuPathDBi | FungiDB:YNL273W |
Phylogenomic databases
eggNOGi | KOG1974, Eukaryota |
GeneTreei | ENSGT00390000015124 |
HOGENOMi | CLU_008440_0_0_1 |
InParanoidi | P53840 |
OMAi | MTVNHHR |
Miscellaneous databases
PROi | PR:P53840 |
RNActi | P53840, protein |
Family and domain databases
InterProi | View protein in InterPro IPR044998, Timeless IPR006906, Timeless_N |
PANTHERi | PTHR22940, PTHR22940, 1 hit |
Pfami | View protein in Pfam PF04821, TIMELESS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TOF1_YEAST | |
Accessioni | P53840Primary (citable) accession number: P53840 Secondary accession number(s): D6W0S1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families