UniProtKB - P53810 (PIPNA_MOUSE)
Protein
Phosphatidylinositol transfer protein alpha isoform
Gene
Pitpna
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes (PubMed:8049244). Shows a preference for PI and PC containing shorter saturated or monosaturated acyl chains at the sn-1 and sn-2 positions (By similarity). Preference order for PC is C16:1 > C16:0 > C18:1 > C18:0 > C20:4 and for PI is C16:1 > C16:0 > C18:1 > C18:0 > C20:4 > C20:3 (By similarity).By similarity1 Publication
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)1 PublicationThis reaction proceeds in the forward1 Publication direction.
- a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)1 PublicationThis reaction proceeds in the forward1 Publication direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 59 | PhosphatidylinositolBy similarity | 1 | |
Binding sitei | 61 | PhosphatidylinositolBy similarity | 1 | |
Binding sitei | 86 | PhosphatidylinositolBy similarity | 1 | |
Binding sitei | 90 | PhosphatidylinositolBy similarity | 1 | |
Binding sitei | 97 | PhosphatidylinositolBy similarity | 1 | |
Binding sitei | 195 | PhosphatidylinositolBy similarity | 1 |
GO - Molecular functioni
- fatty-acyl-CoA binding Source: MGI
- lipid binding Source: MGI
- phosphatidylcholine binding Source: UniProtKB
- phosphatidylcholine transfer activity Source: UniProtKB
- phosphatidylcholine transporter activity Source: MGI
- phosphatidylinositol binding Source: UniProtKB
- phosphatidylinositol transfer activity Source: UniProtKB
- phospholipid binding Source: MGI
- stearic acid binding Source: MGI
GO - Biological processi
- axonogenesis Source: BHF-UCL
- phospholipid transport Source: MGI
Keywordsi
Biological process | Transport |
Ligand | Lipid-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatidylinositol transfer protein alpha isoformShort name: PI-TP-alpha Short name: PtdIns transfer protein alpha Short name: PtdInsTP alpha |
Gene namesi | Name:Pitpna Synonyms:Pitpn |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99887, Pitpna |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- integral component of membrane Source: MGI
- myelin sheath Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Involvement in diseasei
Defects in Pitpna are the cause of the vibrator phenotype which is characterized by early-onset progressive action tremor, degeneration of brain stem and spinal cord neurons, and juvenile death. The mutation is due to the insertion of an intracisternal A particle retrotransposon in intron 4 which results in a 5-fold reduction in protein levels.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000191640 | 2 – 271 | Phosphatidylinositol transfer protein alpha isoformAdd BLAST | 270 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 216 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Phosphorylated by PKC in a calcium and phosphatidylserine-dependent manner.1 Publication
Keywords - PTMi
AcetylationProteomic databases
EPDi | P53810 |
jPOSTi | P53810 |
PaxDbi | P53810 |
PeptideAtlasi | P53810 |
PRIDEi | P53810 |
ProteomicsDBi | 289578 |
2D gel databases
REPRODUCTION-2DPAGEi | P53810 |
PTM databases
iPTMneti | P53810 |
PhosphoSitePlusi | P53810 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000017781, Expressed in jejunum and 313 other tissues |
ExpressionAtlasi | P53810, baseline and differential |
Genevisiblei | P53810, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 202184, 5 interactors |
IntActi | P53810, 1 interactor |
MINTi | P53810 |
STRINGi | 10090.ENSMUSP00000115723 |
Miscellaneous databases
RNActi | P53810, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P53810 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P53810 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3668, Eukaryota |
GeneTreei | ENSGT00940000157119 |
HOGENOMi | CLU_046509_0_0_1 |
InParanoidi | P53810 |
OrthoDBi | 951268at2759 |
PhylomeDBi | P53810 |
TreeFami | TF313279 |
Family and domain databases
Gene3Di | 3.30.530.20, 1 hit |
InterProi | View protein in InterPro IPR001666, PI_transfer IPR023393, START-like_dom_sf |
PANTHERi | PTHR10658, PTHR10658, 1 hit |
Pfami | View protein in Pfam PF02121, IP_trans, 1 hit |
PRINTSi | PR00391, PITRANSFER |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P53810-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVLLKEYRVI LPVSVDEYQV GQLYSVAEAS KNETGGGEGV EVLVNEPYEK
60 70 80 90 100
DDGEKGQYTH KIYHLQSKVP TFVRMLAPEG ALNIHEKAWN AYPYCRTVIT
110 120 130 140 150
NEYMKEDFLI KIETWHKPDL GTQENVHKLE PEAWKHVEAI YIDIADRSQV
160 170 180 190 200
LSKDYKAEED PAKFKSVKTG RGPLGPNWKQ ELVNQKDCPY MCAYKLVTVK
210 220 230 240 250
FKWWGLQNKV ENFIHKQEKR LFTNFHRQLF CWLDKWVDLT MDDIRRMEEE
260 270
TKRQLDEMRQ KDPVKGMTAD D
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3QPW1 | J3QPW1_MOUSE | Phosphatidylinositol transfer prote... | Pitpna | 272 | Annotation score: | ||
J3QQ30 | J3QQ30_MOUSE | Phosphatidylinositol transfer prote... | Pitpna | 271 | Annotation score: | ||
F8WGG5 | F8WGG5_MOUSE | Phosphatidylinositol transfer prote... | Pitpna | 99 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S72681 mRNA Translation: AAC60690.1 U96725 mRNA Translation: AAC53266.1 U96726 Genomic DNA Translation: AAC60756.1 BC056171 mRNA Translation: AAH56171.1 |
CCDSi | CCDS25052.1 |
RefSeqi | NP_032876.1, NM_008850.2 |
Genome annotation databases
Ensembli | ENSMUST00000143219; ENSMUSP00000115723; ENSMUSG00000017781 |
GeneIDi | 18738 |
KEGGi | mmu:18738 |
UCSCi | uc007kei.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S72681 mRNA Translation: AAC60690.1 U96725 mRNA Translation: AAC53266.1 U96726 Genomic DNA Translation: AAC60756.1 BC056171 mRNA Translation: AAH56171.1 |
CCDSi | CCDS25052.1 |
RefSeqi | NP_032876.1, NM_008850.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KCM | X-ray | 2.00 | A | 2-271 | [»] | |
SMRi | P53810 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202184, 5 interactors |
IntActi | P53810, 1 interactor |
MINTi | P53810 |
STRINGi | 10090.ENSMUSP00000115723 |
PTM databases
iPTMneti | P53810 |
PhosphoSitePlusi | P53810 |
2D gel databases
REPRODUCTION-2DPAGEi | P53810 |
Proteomic databases
EPDi | P53810 |
jPOSTi | P53810 |
PaxDbi | P53810 |
PeptideAtlasi | P53810 |
PRIDEi | P53810 |
ProteomicsDBi | 289578 |
Protocols and materials databases
Antibodypediai | 5311, 183 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000143219; ENSMUSP00000115723; ENSMUSG00000017781 |
GeneIDi | 18738 |
KEGGi | mmu:18738 |
UCSCi | uc007kei.1, mouse |
Organism-specific databases
CTDi | 5306 |
MGIi | MGI:99887, Pitpna |
Phylogenomic databases
eggNOGi | KOG3668, Eukaryota |
GeneTreei | ENSGT00940000157119 |
HOGENOMi | CLU_046509_0_0_1 |
InParanoidi | P53810 |
OrthoDBi | 951268at2759 |
PhylomeDBi | P53810 |
TreeFami | TF313279 |
Miscellaneous databases
BioGRID-ORCSi | 18738, 2 hits in 53 CRISPR screens |
ChiTaRSi | Pitpna, mouse |
EvolutionaryTracei | P53810 |
PROi | PR:P53810 |
RNActi | P53810, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000017781, Expressed in jejunum and 313 other tissues |
ExpressionAtlasi | P53810, baseline and differential |
Genevisiblei | P53810, MM |
Family and domain databases
Gene3Di | 3.30.530.20, 1 hit |
InterProi | View protein in InterPro IPR001666, PI_transfer IPR023393, START-like_dom_sf |
PANTHERi | PTHR10658, PTHR10658, 1 hit |
Pfami | View protein in Pfam PF02121, IP_trans, 1 hit |
PRINTSi | PR00391, PITRANSFER |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PIPNA_MOUSE | |
Accessioni | P53810Primary (citable) accession number: P53810 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 162 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families