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Protein

Squalene synthase

Gene

Fdft1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Critical branch point enzyme of isoprenoid biosynthesis that is thought to regulate the flux of isoprene intermediates through the sterol pathway.

Catalytic activityi

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

Cofactori

Pathwayi: lanosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase (Fdft1)
  2. Squalene monooxygenase (Sqle)
  3. Lanosterol synthase (Lss)
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMultifunctional enzyme, Transferase
Biological processCholesterol biosynthesis, Cholesterol metabolism, Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandMagnesium, NADP

Enzyme and pathway databases

ReactomeiR-MMU-191273 Cholesterol biosynthesis
UniPathwayi
UPA00767;UER00751

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene synthase (EC:2.5.1.21)
Short name:
SQS
Short name:
SS
Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase
Gene namesi
Name:Fdft1
Synonyms:Erg9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:102706 Fdft1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei284 – 304HelicalSequence analysisAdd BLAST21
Transmembranei384 – 404HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4778

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000674441 – 416Squalene synthaseAdd BLAST416

Proteomic databases

EPDiP53798
MaxQBiP53798
PaxDbiP53798
PeptideAtlasiP53798
PRIDEiP53798

PTM databases

iPTMnetiP53798
PhosphoSitePlusiP53798
SwissPalmiP53798

Expressioni

Gene expression databases

BgeeiENSMUSG00000021273 Expressed in 99 organ(s), highest expression level in dorsal root ganglion
CleanExiMM_FDFT1
GenevisibleiP53798 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055313

Structurei

3D structure databases

ProteinModelPortaliP53798
SMRiP53798
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1459 Eukaryota
COG1562 LUCA
GeneTreeiENSGT00390000016034
HOGENOMiHOG000186940
HOVERGENiHBG002370
InParanoidiP53798
KOiK00801
OMAiFNFVAIP
OrthoDBiEOG091G072P
TreeFamiTF105316

Family and domain databases

CDDicd00683 Trans_IPPS_HH, 1 hit
Gene3Di1.10.600.10, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR002060 Squ/phyt_synthse
IPR006449 Squal_synth-like
IPR019845 Squalene/phytoene_synthase_CS
IPR033904 Trans_IPPS_HH
PfamiView protein in Pfam
PF00494 SQS_PSY, 1 hit
SUPFAMiSSF48576 SSF48576, 1 hit
TIGRFAMsiTIGR01559 squal_synth, 1 hit
PROSITEiView protein in PROSITE
PS01044 SQUALEN_PHYTOEN_SYN_1, 1 hit
PS01045 SQUALEN_PHYTOEN_SYN_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

P53798-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEFVKCLGHP EEFYNLLRFR MGGRRNFIPK MDQDSLSSSL KTCYKYLNQT
60 70 80 90 100
SRSFAAVIQA LDGDIRHAIC VFYLVLRALD TVEDDMSISV EKKIPLLCNF
110 120 130 140 150
HTFLYDPEWR FTESKEKDRQ VLEDFPTISL EFRNLAEKYQ TVIDDICHRM
160 170 180 190 200
GCGMAEFVDK DVTSKQDWDK YCHYVAGLVG IGLSRLFSAS EFEDPIVGED
210 220 230 240 250
IECANSMGLF LQKTNIIRDY LEDQQEGRKF WPQEVWGRYI KKLEDFAKPE
260 270 280 290 300
NVDVAVQCLN ELITNTLQHI PDVLTYLSRL RNQSVFNFCA IPQVMAIATL
310 320 330 340 350
AACYNNQQVF KGVVKIRKGQ AVTLMMDATN MPAVKAIIYQ YIEEIYHRIP
360 370 380 390 400
NSDPSSSKTK QVISKIRTQN LPNCQLISRS HYSPIYLSFI MLLAALSWQY
410
LSTLSQVTED YVQREH
Length:416
Mass (Da):48,154
Last modified:July 27, 2011 - v2
Checksum:i12C63625DD4FF92B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A286YD20A0A286YD20_MOUSE
Squalene synthase
Fdft1
135Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149R → Q in BAA06102 (PubMed:7999794).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29016 mRNA Translation: BAA06102.1
AK076062 mRNA Translation: BAC36156.1
AK146766 mRNA Translation: BAE27418.1
AK158633 mRNA Translation: BAE34590.1
AK168629 mRNA Translation: BAE40489.1
AK169317 mRNA Translation: BAE41072.1
CH466535 Genomic DNA Translation: EDL36069.1
BC054722 mRNA Translation: AAH54722.1
BC138301 mRNA Translation: AAI38302.1
BC138302 mRNA Translation: AAI38303.1
CCDSiCCDS27198.1
PIRiS52075
RefSeqiNP_034321.2, NM_010191.3
XP_017171333.1, XM_017315844.1
UniGeneiMm.474432

Genome annotation databases

EnsembliENSMUST00000054963; ENSMUSP00000055313; ENSMUSG00000021273
ENSMUST00000224625; ENSMUSP00000153671; ENSMUSG00000021273
GeneIDi14137
KEGGimmu:14137
UCSCiuc007uhj.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29016 mRNA Translation: BAA06102.1
AK076062 mRNA Translation: BAC36156.1
AK146766 mRNA Translation: BAE27418.1
AK158633 mRNA Translation: BAE34590.1
AK168629 mRNA Translation: BAE40489.1
AK169317 mRNA Translation: BAE41072.1
CH466535 Genomic DNA Translation: EDL36069.1
BC054722 mRNA Translation: AAH54722.1
BC138301 mRNA Translation: AAI38302.1
BC138302 mRNA Translation: AAI38303.1
CCDSiCCDS27198.1
PIRiS52075
RefSeqiNP_034321.2, NM_010191.3
XP_017171333.1, XM_017315844.1
UniGeneiMm.474432

3D structure databases

ProteinModelPortaliP53798
SMRiP53798
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055313

Chemistry databases

ChEMBLiCHEMBL4778

PTM databases

iPTMnetiP53798
PhosphoSitePlusiP53798
SwissPalmiP53798

Proteomic databases

EPDiP53798
MaxQBiP53798
PaxDbiP53798
PeptideAtlasiP53798
PRIDEiP53798

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054963; ENSMUSP00000055313; ENSMUSG00000021273
ENSMUST00000224625; ENSMUSP00000153671; ENSMUSG00000021273
GeneIDi14137
KEGGimmu:14137
UCSCiuc007uhj.3 mouse

Organism-specific databases

CTDi2222
MGIiMGI:102706 Fdft1

Phylogenomic databases

eggNOGiKOG1459 Eukaryota
COG1562 LUCA
GeneTreeiENSGT00390000016034
HOGENOMiHOG000186940
HOVERGENiHBG002370
InParanoidiP53798
KOiK00801
OMAiFNFVAIP
OrthoDBiEOG091G072P
TreeFamiTF105316

Enzyme and pathway databases

UniPathwayi
UPA00767;UER00751

ReactomeiR-MMU-191273 Cholesterol biosynthesis

Miscellaneous databases

ChiTaRSiFdft1 mouse
PROiPR:P53798
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021273 Expressed in 99 organ(s), highest expression level in dorsal root ganglion
CleanExiMM_FDFT1
GenevisibleiP53798 MM

Family and domain databases

CDDicd00683 Trans_IPPS_HH, 1 hit
Gene3Di1.10.600.10, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR002060 Squ/phyt_synthse
IPR006449 Squal_synth-like
IPR019845 Squalene/phytoene_synthase_CS
IPR033904 Trans_IPPS_HH
PfamiView protein in Pfam
PF00494 SQS_PSY, 1 hit
SUPFAMiSSF48576 SSF48576, 1 hit
TIGRFAMsiTIGR01559 squal_synth, 1 hit
PROSITEiView protein in PROSITE
PS01044 SQUALEN_PHYTOEN_SYN_1, 1 hit
PS01045 SQUALEN_PHYTOEN_SYN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFDFT_MOUSE
AccessioniPrimary (citable) accession number: P53798
Secondary accession number(s): Q8BPF5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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