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Protein

Aryl hydrocarbon receptor nuclear translocator

Gene

Arnt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens (By similarity). The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (PubMed:26245371, PubMed:27782878). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28602820).By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-1234158 Regulation of gene expression by Hypoxia-inducible Factor
R-MMU-211945 Phase I - Functionalization of compounds
R-MMU-211976 Endogenous sterols
R-MMU-211981 Xenobiotics
R-MMU-8937144 Aryl hydrocarbon receptor signalling

Names & Taxonomyi

Protein namesi
Recommended name:
Aryl hydrocarbon receptor nuclear translocator
Short name:
ARNT protein
Alternative name(s):
Dioxin receptor, nuclear translocator
Hypoxia-inducible factor 1-beta
Short name:
HIF-1-beta
Short name:
HIF1-beta
Gene namesi
Name:Arnt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:88071 Arnt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi94H → A: Reduces DNA binding. 1 Publication1
Mutagenesisi98E → A: Reduces DNA binding. 1 Publication1
Mutagenesisi102R → E: Reduces DNA binding. Decreases transcription factor activity. 2 Publications1
Mutagenesisi112L → D: Interfers with transcription factor activity. 1 Publication1
Mutagenesisi112L → E: Impairs heterodimer formation with EPAS1. Impairs heterodimer formation with HIF1A. Significantly destabilizes ARNT?s heterodimeric interactions with both NPAS1 and NPAS3. Compromises SIM1:ARNT heterodimer stability. Compromise NPAS4:ARNT heterodimer stability. Compromise AHR:ARNT heterodimer stability. 2 Publications1
Mutagenesisi132L → E: Impairs heterodimer formation with EPAS1. Impairs heterodimer formation with HIF1A. Significantly destabilizes ARNT?s heterodimeric interactions with both NPAS1 and NPAS3. Compromises SIM1:ARNT heterodimer stability. Compromise NPAS4:ARNT heterodimer stability. Compromise AHR:ARNT heterodimer stability. 2 Publications1
Mutagenesisi136V → D: Impairs heterodimer formation with EPAS1. Impairs heterodimer formation with HIF1A. Significantly destabilizes ARNT?s heterodimeric interactions with both NPAS1 and NPAS3. Compromises SIM1:ARNT heterodimer stability. Compromise NPAS4:ARNT heterodimer stability. Compromise AHR:ARNT heterodimer stability. 2 Publications1
Mutagenesisi139M → D: Interfers with transcription factor activity. 1 Publication1
Mutagenesisi164L → D: Does not affect transcription factor activity. 1 Publication1
Mutagenesisi167L → E: Highly reduces transcription activity. Impairs interaction with AHR. Impairs heterodimer formation with EPAS1. Impairs heterodimer formation with HIF1A. Significantly destabilizes ARNT?s heterodimeric interactions with both NPAS1 and NPAS3. Compromises SIM1:ARNT heterodimer stability. Compromise NPAS4:ARNT heterodimer stability. Compromise AHR:ARNT heterodimer stability. 3 Publications1
Mutagenesisi168I → D: Highly reduces transcription activity. Impairs interaction with AHR. Impairs heterodimer formation with EPAS1. Impairs heterodimer formation with HIF1A. Significantly destabilizes ARNT?s heterodimeric interactions with both NPAS1 and NPAS3. Compromises SIM1:ARNT heterodimer stability. Compromise NPAS4:ARNT heterodimer stability. Compromise AHR:ARNT heterodimer stability. 3 Publications1
Mutagenesisi171A → D: Reduces transcription activity. Markedly reduces interaction with AHR. Impairs heterodimer formation with EPAS1. Markedly decreases heterodimer formation with HIF1A. Significantly destabilizes ARNT?s heterodimeric interactions with both NPAS1 and NPAS3. Compromises SIM1:ARNT heterodimer stability. Compromise NPAS4:ARNT heterodimer stability. Compromise AHR:ARNT heterodimer stability. 3 Publications1
Mutagenesisi264I → D: Impairs heterodimer formation with EPAS1. Markedly decreases heterodimer formation with HIF1A. Significantly destabilizes ARNT?s heterodimeric interactions with both NPAS1 and NPAS3. Compromises SIM1:ARNT heterodimer stability. Does not compromise NPAS4:ARNT heterodimer stability. Does not compromise AHR:ARNT heterodimer stability. 2 Publications1
Mutagenesisi266R → A: Markedly decreases heterodimer formation with EPAS1. Decreases heterodimer formation with HIF1A. Significantly destabilizes ARNT?s heterodimeric interactions with both NPAS1 and NPAS3. Compromises SIM1:ARNT heterodimer stability. Does not compromise NPAS4:ARNT heterodimer stability. Does not compromise AHR:ARNT heterodimer stability. 2 Publications1
Mutagenesisi305V → D: Markedly decreases heterodimer formation with EPAS1. Markedly decreases heterodimer formation with HIF1A. Significantly destabilizes ARNT?s heterodimeric interactions with both NPAS1 and NPAS3. Compromises SIM1:ARNT heterodimer stability. Does not compromise NPAS4:ARNT heterodimer stability. Does not compromise AHR:ARNT heterodimer stability. 2 Publications1
Mutagenesisi307H → A: Decreases heterodimer formation with EPAS1. Decreases heterodimer formation with HIF1A. Significantly destabilizes ARNT?s heterodimeric interactions with both NPAS1 and NPAS3. Compromises SIM1:ARNT heterodimer stability. Does not compromise NPAS4:ARNT heterodimer stability. Does not compromise AHR:ARNT heterodimer stability. 2 Publications1
Mutagenesisi366R → A: Markedly decreases heterodimer formation with EPAS1. Markedly decreases heterodimer formation with HIF1A. Impairs heterodimer formation with EPAS1; when associated with N-448. Impairs heterodimer formation with HIF1A; when associated with N-448. Significantly destabilizes ARNT?s heterodimeric interactions with both NPAS1 and NPAS3. Compromises SIM1:ARNT heterodimer stability. Does not compromise NPAS4:ARNT heterodimer stability. Does not compromise AHR:ARNT heterodimer stability. 2 Publications1
Mutagenesisi448N → A: Decreases heterodimer formation with EPAS1. Decreases heterodimer formation with HIF1A. Impairs heterodimer formation with EPAS1; when associated with A-366. Impairs heterodimer formation with HIF1A; when associated with A-366. Significantly destabilizes ARNT?s heterodimeric interactions with both NPAS1 and NPAS3. Compromises SIM1:ARNT heterodimer stability. Does not compromise NPAS4:ARNT heterodimer stability. Does not compromise AHR:ARNT heterodimer stability. 2 Publications1
Mutagenesisi456Y → D: Decreases heterodimer formation with EPAS1. Decreases heterodimer formation with HIF1A. Significantly destabilizes ARNT?s heterodimeric interactions with both NPAS1 and NPAS3. Compromises SIM1:ARNT heterodimer stability. Does not compromise NPAS4:ARNT heterodimer stability. Does not compromise AHR:ARNT heterodimer stability. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001271192 – 791Aryl hydrocarbon receptor nuclear translocatorAdd BLAST790

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki58Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei77PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP53762
PaxDbiP53762
PRIDEiP53762

PTM databases

iPTMnetiP53762
PhosphoSitePlusiP53762

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSMUSG00000015522 Expressed in 314 organ(s), highest expression level in camera-type eye
CleanExiMM_ARNT
ExpressionAtlasiP53762 baseline and differential
GenevisibleiP53762 MM

Interactioni

Subunit structurei

Monomer (PubMed:28602820). Homodimer only upon binding to a DNA (PubMed:26245371, PubMed:28602820). Efficient DNA binding requires dimerization with another bHLH protein (By similarity). Interacts with TACC3 (PubMed:11025203). Interacts with HIF1A, EPAS1, NPAS1 and NPAS3; forms a heterodimer that binds core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:26245371, PubMed:27782878). Forms a heterodimer with AHRR, as well as with other bHLH proteins (PubMed:9887096). Interacts with NOCA7 (By similarity). Interacts with AHR; the heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28602820, PubMed:24001774, PubMed:27782878). Interacts with SIM1 and NPAS4 (PubMed:27782878).By similarity6 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198205, 9 interactors
CORUMiP53762
DIPiDIP-280N
IntActiP53762, 12 interactors
STRINGi10090.ENSMUSP00000099810

Structurei

Secondary structure

1791
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP53762
SMRiP53762
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini89 – 142bHLHPROSITE-ProRule annotationAdd BLAST54
Domaini161 – 235PAS 1PROSITE-ProRule annotationAdd BLAST75
Domaini349 – 419PAS 2PROSITE-ProRule annotationAdd BLAST71
Domaini424 – 467PACAdd BLAST44

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni88 – 128DNA-binding2 PublicationsAdd BLAST41
Regioni112 – 264Required for heterodimer formation with EPAS11 PublicationAdd BLAST153
Regioni112 – 168Required for heterodimer formation with HIF1A1 PublicationAdd BLAST57
Regioni167 – 171Mediates the transcription activity and dimerization of the AHR:ARNT complex1 Publication5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi99 – 102Poly-Arg4
Compositional biasi503 – 507Poly-Gln5
Compositional biasi740 – 743Poly-Ser4

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IQ5T Eukaryota
ENOG410XVHF LUCA
GeneTreeiENSGT00760000118788
HOGENOMiHOG000234380
HOVERGENiHBG000164
InParanoidiP53762
KOiK09097
OrthoDBiEOG091G03AI
TreeFamiTF319983

Family and domain databases

CDDicd00083 HLH, 1 hit
cd00130 PAS, 2 hits
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR001067 Nuc_translocat
IPR001610 PAC
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
PfamiView protein in Pfam
PF00010 HLH, 1 hit
PF00989 PAS, 1 hit
PRINTSiPR00785 NCTRNSLOCATR
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SM00086 PAC, 1 hit
SM00091 PAS, 2 hits
SUPFAMiSSF47459 SSF47459, 1 hit
SSF55785 SSF55785, 2 hits
TIGRFAMsiTIGR00229 sensory_box, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS50112 PAS, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform Long (identifier: P53762-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAATTANPEM TSDVPSLGPT IASGNPGPGI QGGGAVVQRA IKRRSGLDFD
60 70 80 90 100
DEVEVNTKFL RCDDDQMCND KERFARSDDE QSSADKERLA RENHSEIERR
110 120 130 140 150
RRNKMTAYIT ELSDMVPTCS ALARKPDKLT ILRMAVSHMK SLRGTGNTST
160 170 180 190 200
DGSYKPSFLT DQELKHLILE AADGFLFIVS CETGRVVYVS DSVTPVLNQP
210 220 230 240 250
QSEWFGSTLY DQVHPDDVDK LREQLSTSEN ALTGRVLDLK TGTVKKEGQQ
260 270 280 290 300
SSMRMCMGSR RSFICRMRCG TSSVDPVSMN RLSFLRNRCR NGLGSVKEGE
310 320 330 340 350
PHFVVVHCTG YIKAWPPAGV SLPDDDPEAG QGSKFCLVAI GRLQVTSSPN
360 370 380 390 400
CTDMSNICQP TEFISRHNIE GIFTFVDHRC VATVGYQPQE LLGKNIVEFC
410 420 430 440 450
HPEDQQLLRD SFQQVVKLKG QVLSVMFRFR SKTREWLWMR TSSFTFQNPY
460 470 480 490 500
SDEIEYIICT NTNVKNSSQE PRPTLSNTIP RSQLGPTANL SLEMGTGQLP
510 520 530 540 550
SRQQQQQHTE LDMVPGRDGL ASYNHSQVSV QPVASAGSEH SKPLEKSEGL
560 570 580 590 600
FAQDRDPRFP EIYPSITADQ SKGISSSTVP ATQQLFSQGS SFPPNPRPAE
610 620 630 640 650
NFRNSGLTPP VTIVQPSSSA GQILAQISRH SNPAQGSAPT WTSSSRPGFA
660 670 680 690 700
AQQVPTQATA KTRSSQFGVN NFQTSSSFSA MSLPGAPTAS SGTAAYPALP
710 720 730 740 750
NRGSNFPPET GQTTGQFQAR TAEGVGVWPQ WQGQQPHHRS SSSEQHVQQT
760 770 780 790
QAQAPSQPEV FQEMLSMLGD QSNTYNNEEF PDLTMFPPFS E
Length:791
Mass (Da):86,963
Last modified:July 27, 2011 - v3
Checksum:iBDEC0991CD57D402
GO
Isoform Short (identifier: P53762-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-100: SDDEQSSADKERLARENHSEIERR → TKFL

Show »
Length:771
Mass (Da):84,613
Checksum:i1577F629BAE8F38B
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3ULM2Q3ULM2_MOUSE
Aryl hydrocarbon receptor nuclear t...
Arnt
776Annotation score:
E9QLT6E9QLT6_MOUSE
Aryl hydrocarbon receptor nuclear t...
Arnt
771Annotation score:
Q8CEC2Q8CEC2_MOUSE
Aryl hydrocarbon receptor nuclear t...
Arnt
760Annotation score:
F7C9F6F7C9F6_MOUSE
Aryl hydrocarbon receptor nuclear t...
Arnt
126Annotation score:
D3YV20D3YV20_MOUSE
Aryl hydrocarbon receptor nuclear t...
Arnt
76Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti411S → T in AAA61732 (PubMed:7961746).Curated1
Sequence conflicti534A → R in AAA61732 (PubMed:7961746).Curated1
Sequence conflicti644S → T in AAA56717 (PubMed:8065341).Curated1
Sequence conflicti650A → C in AAA61732 (PubMed:7961746).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00209377 – 100SDDEQ…EIERR → TKFL in isoform Short. 1 PublicationAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10325 mRNA Translation: AAA56717.1
U14333 mRNA Translation: AAA61732.1
AK153355 mRNA Translation: BAE31928.1
CH466620 Genomic DNA Translation: EDL38816.1
BC012870 mRNA Translation: AAH12870.1
CCDSiCCDS17614.1 [P53762-1]
PIRiA55448
A56241
RefSeqiNP_001032826.1, NM_001037737.2 [P53762-1]
UniGeneiMm.250265

Genome annotation databases

EnsembliENSMUST00000102749; ENSMUSP00000099810; ENSMUSG00000015522 [P53762-1]
GeneIDi11863
KEGGimmu:11863
UCSCiuc008qjr.2 mouse [P53762-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10325 mRNA Translation: AAA56717.1
U14333 mRNA Translation: AAA61732.1
AK153355 mRNA Translation: BAE31928.1
CH466620 Genomic DNA Translation: EDL38816.1
BC012870 mRNA Translation: AAH12870.1
CCDSiCCDS17614.1 [P53762-1]
PIRiA55448
A56241
RefSeqiNP_001032826.1, NM_001037737.2 [P53762-1]
UniGeneiMm.250265

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZP4X-ray2.35A/C82-464[»]
4ZPHX-ray2.80A/C82-464[»]
4ZPKX-ray3.60A82-464[»]
4ZPRX-ray3.90A82-464[»]
4ZQDX-ray2.87A/C82-464[»]
5NJ8X-ray3.30B/D84-345[»]
5SY5X-ray3.20A/C/E82-464[»]
5SY7X-ray4.20A82-464[»]
ProteinModelPortaliP53762
SMRiP53762
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198205, 9 interactors
CORUMiP53762
DIPiDIP-280N
IntActiP53762, 12 interactors
STRINGi10090.ENSMUSP00000099810

PTM databases

iPTMnetiP53762
PhosphoSitePlusiP53762

Proteomic databases

MaxQBiP53762
PaxDbiP53762
PRIDEiP53762

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102749; ENSMUSP00000099810; ENSMUSG00000015522 [P53762-1]
GeneIDi11863
KEGGimmu:11863
UCSCiuc008qjr.2 mouse [P53762-1]

Organism-specific databases

CTDi405
MGIiMGI:88071 Arnt

Phylogenomic databases

eggNOGiENOG410IQ5T Eukaryota
ENOG410XVHF LUCA
GeneTreeiENSGT00760000118788
HOGENOMiHOG000234380
HOVERGENiHBG000164
InParanoidiP53762
KOiK09097
OrthoDBiEOG091G03AI
TreeFamiTF319983

Enzyme and pathway databases

ReactomeiR-MMU-1234158 Regulation of gene expression by Hypoxia-inducible Factor
R-MMU-211945 Phase I - Functionalization of compounds
R-MMU-211976 Endogenous sterols
R-MMU-211981 Xenobiotics
R-MMU-8937144 Aryl hydrocarbon receptor signalling

Miscellaneous databases

ChiTaRSiArnt mouse
PROiPR:P53762
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015522 Expressed in 314 organ(s), highest expression level in camera-type eye
CleanExiMM_ARNT
ExpressionAtlasiP53762 baseline and differential
GenevisibleiP53762 MM

Family and domain databases

CDDicd00083 HLH, 1 hit
cd00130 PAS, 2 hits
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR001067 Nuc_translocat
IPR001610 PAC
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
PfamiView protein in Pfam
PF00010 HLH, 1 hit
PF00989 PAS, 1 hit
PRINTSiPR00785 NCTRNSLOCATR
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SM00086 PAC, 1 hit
SM00091 PAS, 2 hits
SUPFAMiSSF47459 SSF47459, 1 hit
SSF55785 SSF55785, 2 hits
TIGRFAMsiTIGR00229 sensory_box, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS50112 PAS, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiARNT_MOUSE
AccessioniPrimary (citable) accession number: P53762
Secondary accession number(s): Q60661, Q921F3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 168 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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