UniProtKB - P53746 (FRE4_YEAST)
Protein
Ferric reductase transmembrane component 4
Gene
FRE4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Siderophore-iron reductase responsible for reducing extracellular iron prior to import. Catalyzes the reductive uptake of Fe3+ bound to dihydroxamate rhodotorulic acid. Fe3+ is reduced to Fe2+, which then dissociates from the siderophore and can be imported by the high-affinity Fe2+ transport complex in the plasma membrane.1 Publication
Catalytic activityi
- EC:1.16.1.9
Cofactori
FADCurated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 309 | Iron (heme 1 axial ligand)By similarity | 1 | |
Metal bindingi | 323 | Iron (heme 2 axial ligand)By similarity | 1 | |
Metal bindingi | 379 | Iron (heme 1 axial ligand)By similarity | 1 | |
Metal bindingi | 393 | Iron (heme 2 axial ligand)By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 472 – 478 | FADSequence analysis | 7 | |
Nucleotide bindingi | 519 – 522 | NADPSequence analysis | 4 | |
Nucleotide bindingi | 685 – 686 | NADPSequence analysis | 2 |
GO - Molecular functioni
- ferric-chelate reductase (NADPH) activity Source: UniProtKB-EC
- ferric-chelate reductase activity Source: SGD
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cellular iron ion homeostasis Source: GO_Central
- copper ion import Source: GO_Central
- iron ion transport Source: GO_Central
- siderophore transport Source: SGD
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Ion transport, Iron transport, Transport |
Ligand | FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP |
Names & Taxonomyi
Protein namesi | Recommended name: Ferric reductase transmembrane component 4 (EC:1.16.1.9)Alternative name(s): Ferric-chelate reductase 4 |
Gene namesi | Name:FRE4 Ordered Locus Names:YNR060W ORF Names:N3518 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YNR060W |
SGDi | S000005343, FRE4 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein Curated
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 19 – 156 | ExtracellularBy similarityAdd BLAST | 138 | |
Transmembranei | 157 – 177 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 178 – 228 | CytoplasmicBy similarityAdd BLAST | 51 | |
Transmembranei | 229 – 249 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 250 – 267 | ExtracellularBy similarityAdd BLAST | 18 | |
Transmembranei | 268 – 288 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 289 – 304 | CytoplasmicBy similarityAdd BLAST | 16 | |
Transmembranei | 305 – 325 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 326 – 346 | ExtracellularBy similarityAdd BLAST | 21 | |
Transmembranei | 347 – 367 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 368 – 373 | CytoplasmicBy similarity | 6 | |
Transmembranei | 374 – 394 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 395 | ExtracellularBy similarity | 1 | |
Transmembranei | 396 – 416 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 417 – 719 | CytoplasmicBy similarityAdd BLAST | 303 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000010140 | 19 – 719 | Ferric reductase transmembrane component 4Add BLAST | 701 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 51 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 80 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 101 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 113 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 127 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 135 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | P53746 |
PRIDEi | P53746 |
PTM databases
iPTMneti | P53746 |
Expressioni
Inductioni
By iron deprivation.2 Publications
Interactioni
Protein-protein interaction databases
BioGRIDi | 35886, 24 interactors |
DIPi | DIP-7626N |
IntActi | P53746, 1 interactor |
MINTi | P53746 |
STRINGi | 4932.YNR060W |
Miscellaneous databases
RNActi | P53746, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 273 – 407 | Ferric oxidoreductaseAdd BLAST | 135 | |
Domaini | 408 – 527 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 120 |
Sequence similaritiesi
Belongs to the ferric reductase (FRE) family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0039, Eukaryota |
GeneTreei | ENSGT00940000176303 |
HOGENOMi | CLU_010365_4_0_1 |
InParanoidi | P53746 |
OMAi | GWSYRTF |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P53746-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLLVHIISFL LFFQLSAAKA PPSKTSLINT HERRSIYSCY VGLRKETWGF
60 70 80 90 100
NGSAICRYEP AIQSMLYCLY EDTHEKGYSN KTLEKGFEEM RQFCYTPKFL
110 120 130 140 150
NMTDAEFYTS LDNGTYYIQD QPKAGINITY PIRLNTTLRK AYYDAYYGYY
160 170 180 190 200
YNHDIPYYFG GIICAYFVGV MLLAGLIRFL NYTPIKKIMF QQKLVNYVRG
210 220 230 240 250
YTTLPTLYEK HAEPFSYLKV ITGYLPTRFE TLVILGYLIL HTIFMAYKYQ
260 270 280 290 300
YDPYHIIFAA HRAEVAHFVA YRSGILSFAH LPLIVLFAGR NNFLQLISGL
310 320 330 340 350
KHTSFIVFHK WLGRMMFLDA IIHAAGFTNY YLYYKKWNTV RLRVYWKFGI
360 370 380 390 400
ATTCLAGMLI FFSIAAFRRH YYETFMALHI VFAALFLYTC WEHVTNFSGI
410 420 430 440 450
EWIYAAIAIW GVDRIVRITR IALLGFPKAD LQLVGSDLVR VTVKKPKKFW
460 470 480 490 500
KAKPGQYVFV SFLRPLCFWQ SHPFTVMDSC VNDRELVIVL KAKKGVTKLV
510 520 530 540 550
RNFVERKGGK ASMRLAIEGP YGSKSTAHRF DNVLLLAGGS GLPGPISHAL
560 570 580 590 600
ELGKTTAASG KNFVQLVIAV RGLDMLNACK KELMALKGLN VQVHIYNSKQ
610 620 630 640 650
ELASAEKISS NEVKNGETTA EKAPSSLSNS EKAPSESENT ELPLSLNDTS
660 670 680 690 700
ISDLEFATFH VGRPNVEEIL NESVNHSGSL AVVCCGPPIF VDTARNQTAK
710
AVIRNPSRMI EYLEEYQAW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z71675 Genomic DNA Translation: CAA96342.1 BK006947 Genomic DNA Translation: DAA10601.1 |
PIRi | S63392 |
RefSeqi | NP_014458.1, NM_001183237.1 |
Genome annotation databases
EnsemblFungii | YNR060W_mRNA; YNR060W; YNR060W |
GeneIDi | 855797 |
KEGGi | sce:YNR060W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z71675 Genomic DNA Translation: CAA96342.1 BK006947 Genomic DNA Translation: DAA10601.1 |
PIRi | S63392 |
RefSeqi | NP_014458.1, NM_001183237.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 35886, 24 interactors |
DIPi | DIP-7626N |
IntActi | P53746, 1 interactor |
MINTi | P53746 |
STRINGi | 4932.YNR060W |
PTM databases
iPTMneti | P53746 |
Proteomic databases
PaxDbi | P53746 |
PRIDEi | P53746 |
Genome annotation databases
EnsemblFungii | YNR060W_mRNA; YNR060W; YNR060W |
GeneIDi | 855797 |
KEGGi | sce:YNR060W |
Organism-specific databases
EuPathDBi | FungiDB:YNR060W |
SGDi | S000005343, FRE4 |
Phylogenomic databases
eggNOGi | KOG0039, Eukaryota |
GeneTreei | ENSGT00940000176303 |
HOGENOMi | CLU_010365_4_0_1 |
InParanoidi | P53746 |
OMAi | GWSYRTF |
Miscellaneous databases
PROi | PR:P53746 |
RNActi | P53746, protein |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FRE4_YEAST | |
Accessioni | P53746Primary (citable) accession number: P53746 Secondary accession number(s): D6W1N5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | October 7, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD