UniProtKB - P53725 (MPP6_YEAST)
Protein
M-phase phosphoprotein 6 homolog
Gene
MPP6
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA) derived from intergenic regions and the ribosomal DNA spacer heterochromatin. Binds RNA.1 Publication
Miscellaneous
Present with 1350 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- poly(U) RNA binding Source: SGD
- protein-containing complex binding Source: SGD
GO - Biological processi
- exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
- nuclear mRNA surveillance of mRNA 3'-end processing Source: SGD
- nuclear mRNA surveillance of spliceosomal pre-mRNA splicing Source: SGD
- nuclear polyadenylation-dependent CUT catabolic process Source: SGD
- nuclear polyadenylation-dependent rRNA catabolic process Source: SGD
Keywordsi
Molecular function | RNA-binding |
Biological process | rRNA processing |
Names & Taxonomyi
Protein namesi | Recommended name: M-phase phosphoprotein 6 homologAlternative name(s): Exosome-associated RNA-binding protein MPP6 |
Gene namesi | Name:MPP6 Ordered Locus Names:YNR024W ORF Names:N3230 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005307, MPP6 |
VEuPathDBi | FungiDB:YNR024W |
Subcellular locationi
Nucleus
- Nucleus 4 Publications
Note: Colocalizes with the nuclear exosome and ribosomes.
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Lethal in combination with the absence of either RRP6 or its cofactor RRP47. Weak but detectable stabilization of unspliced pre-mRNAs, and stronger stabilization is detected for mRNAs with defects in 3' cleavage and polyadenylation.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000203474 | 2 – 186 | M-phase phosphoprotein 6 homologAdd BLAST | 185 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 42 | PhosphoserineCombined sources | 1 | |
Modified residuei | 150 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P53725 |
PaxDbi | P53725 |
PRIDEi | P53725 |
PTM databases
iPTMneti | P53725 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 35849, 95 interactors |
DIPi | DIP-4263N |
IntActi | P53725, 1 interactor |
MINTi | P53725 |
STRINGi | 4932.YNR024W |
Miscellaneous databases
RNActi | P53725, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P53725 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG502SFA3, Eukaryota |
HOGENOMi | CLU_132767_0_0_1 |
InParanoidi | P53725 |
OMAi | KIMENVG |
Family and domain databases
InterProi | View protein in InterPro IPR019324, MPP6 |
Pfami | View protein in Pfam PF10175, MPP6, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P53725-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSANNGVTGK LSSRVMNMKF MKFGKTDDEE SSNSNTPSNI NSDVEPIEQK
60 70 80 90 100
GKLFGLDDSA WDLNSYKDDL KKISGKEKKK VKRVVYKKRP NLIISNVGYS
110 120 130 140 150
ELRKPEGVIS GRKTFGDNSD DSGSRKRKFD EGEQNEDEKR DAKDKEFTGS
160 170 180
QDDGEDEYDL DKLFKDSIKK KKTNHNGKNK NRNSKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z71639 Genomic DNA Translation: CAA96303.1 BK006947 Genomic DNA Translation: DAA10565.1 |
PIRi | S63355 |
RefSeqi | NP_014421.3, NM_001183201.3 |
Genome annotation databases
EnsemblFungii | YNR024W_mRNA; YNR024W; YNR024W |
GeneIDi | 855758 |
KEGGi | sce:YNR024W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z71639 Genomic DNA Translation: CAA96303.1 BK006947 Genomic DNA Translation: DAA10565.1 |
PIRi | S63355 |
RefSeqi | NP_014421.3, NM_001183201.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5OKZ | X-ray | 3.20 | J/T/d/n | 1-186 | [»] | |
5VZJ | X-ray | 3.30 | L | 81-120 | [»] | |
6FSZ | electron microscopy | 4.60 | NN | 90-118 | [»] | |
6LQS | electron microscopy | 3.80 | M6 | 1-186 | [»] | |
SMRi | P53725 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35849, 95 interactors |
DIPi | DIP-4263N |
IntActi | P53725, 1 interactor |
MINTi | P53725 |
STRINGi | 4932.YNR024W |
PTM databases
iPTMneti | P53725 |
Proteomic databases
MaxQBi | P53725 |
PaxDbi | P53725 |
PRIDEi | P53725 |
Genome annotation databases
EnsemblFungii | YNR024W_mRNA; YNR024W; YNR024W |
GeneIDi | 855758 |
KEGGi | sce:YNR024W |
Organism-specific databases
SGDi | S000005307, MPP6 |
VEuPathDBi | FungiDB:YNR024W |
Phylogenomic databases
eggNOGi | ENOG502SFA3, Eukaryota |
HOGENOMi | CLU_132767_0_0_1 |
InParanoidi | P53725 |
OMAi | KIMENVG |
Miscellaneous databases
PROi | PR:P53725 |
RNActi | P53725, protein |
Family and domain databases
InterProi | View protein in InterPro IPR019324, MPP6 |
Pfami | View protein in Pfam PF10175, MPP6, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MPP6_YEAST | |
Accessioni | P53725Primary (citable) accession number: P53725 Secondary accession number(s): D6W1J9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references