UniProtKB - P53690 (MMP14_MOUSE)
Protein
Matrix metalloproteinase-14
Gene
Mmp14
Organism
Mus musculus (Mouse)
Status
Functioni
Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (PubMed:10520996). May be involved in actin cytoskeleton reorganization by cleaving PTK7 (By similarity). Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues (By similarity). Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis (By similarity).By similarity1 Publication
Catalytic activityi
- Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain. EC:3.4.24.80
Cofactori
Protein has several cofactor binding sites:Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 93 | Zinc; in inhibited formBy similarity | 1 | |
Metal bindingi | 239 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 240 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 243 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 249 | Zinc; catalyticPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- endopeptidase activity Source: MGI
- integrin binding Source: MGI
- metalloaminopeptidase activity Source: MGI
- metalloendopeptidase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- angiogenesis Source: Ensembl
- astrocyte cell migration Source: Ensembl
- bone development Source: MGI
- branching morphogenesis of an epithelial tube Source: MGI
- cell migration Source: MGI
- chondrocyte proliferation Source: MGI
- collagen catabolic process Source: MGI
- craniofacial suture morphogenesis Source: MGI
- embryonic cranial skeleton morphogenesis Source: MGI
- endochondral ossification Source: MGI
- endodermal cell differentiation Source: Ensembl
- endothelial cell proliferation Source: Ensembl
- extracellular matrix organization Source: GO_Central
- head development Source: MGI
- lung development Source: MGI
- male gonad development Source: Ensembl
- negative regulation of focal adhesion assembly Source: MGI
- negative regulation of Notch signaling pathway Source: UniProtKB
- ossification Source: MGI
- ovarian follicle development Source: Ensembl
- positive regulation of B cell differentiation Source: UniProtKB
- positive regulation of cell growth Source: MGI
- positive regulation of cell migration Source: MGI
- positive regulation of macrophage migration Source: BHF-UCL
- positive regulation of myotube differentiation Source: UniProtKB
- positive regulation of protein processing Source: MGI
- proteolysis Source: MGI
- regulation of protein localization to plasma membrane Source: MGI
- response to estrogen Source: Ensembl
- response to hormone Source: MGI
- response to hypoxia Source: Ensembl
- response to mechanical stimulus Source: Ensembl
- response to odorant Source: Ensembl
- response to organic cyclic compound Source: Ensembl
- response to oxidative stress Source: Ensembl
- skeletal system development Source: GO_Central
- tissue remodeling Source: MGI
- zymogen activation Source: MGI
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Biological process | Collagen degradation |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-1442490, Collagen degradation R-MMU-1474228, Degradation of the extracellular matrix R-MMU-1592389, Activation of Matrix Metalloproteinases |
Protein family/group databases
MEROPSi | M10.014 |
Names & Taxonomyi
Protein namesi | Recommended name: Matrix metalloproteinase-14 (EC:3.4.24.80)Short name: MMP-14 Alternative name(s): MMP-X1 MT-MMP Membrane-type matrix metalloproteinase 1 Short name: MT-MMP 1 Short name: MTMMP1 Membrane-type-1 matrix metalloproteinase Short name: MT1-MMP Short name: MT1MMP |
Gene namesi | Name:Mmp14 Synonyms:Mtmmp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101900, Mmp14 |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass type I membrane protein Curated; Extracellular side Curated
- Melanosome By similarity
- Cytoplasm By similarity
Note: Forms a complex with BST2 and localizes to the cytoplasm.By similarity
Cytoskeleton
- intermediate filament cytoskeleton Source: MGI
Cytosol
- cytosol Source: MGI
Endosome
- macropinosome Source: MGI
Extracellular region or secreted
- extracellular matrix Source: InterPro
- extracellular space Source: MGI
Nucleus
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: MGI
- cytoplasmic vesicle Source: MGI
- integral component of membrane Source: UniProtKB-KW
- melanosome Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 112 – 541 | ExtracellularSequence analysisAdd BLAST | 430 | |
Transmembranei | 542 – 562 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 563 – 582 | CytoplasmicSequence analysisAdd BLAST | 20 |
Keywords - Cellular componenti
Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
PropeptideiPRO_0000028800 | 21 – 111 | By similarityAdd BLAST | 91 | |
ChainiPRO_0000028801 | 112 – 582 | Matrix metalloproteinase-14Add BLAST | 471 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 319 ↔ 508 | By similarity | ||
Modified residuei | 399 | Phosphotyrosine; by PKDCCBy similarity | 1 |
Post-translational modificationi
The precursor is cleaved by a furin endopeptidase.By similarity
Tyrosine phosphorylated by PKDCC/VLK.By similarity
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Phosphoprotein, ZymogenProteomic databases
MaxQBi | P53690 |
PaxDbi | P53690 |
PeptideAtlasi | P53690 |
PRIDEi | P53690 |
PTM databases
iPTMneti | P53690 |
PhosphoSitePlusi | P53690 |
SwissPalmi | P53690 |
Expressioni
Tissue specificityi
Highly expressed in placenta, kidney, heart, lung, embryonic skeletal and periskeletal tissues.
Developmental stagei
Not detected before day 10.5. At day 12.5, prominently expressed in large arteries and the umbilical arteries, expressed at lower levels in the myocardium, craniofacial mesenchyme, nasal epithelium and liver capsule. At days 14.5 and 17.5, expressed in the musculoskeletal system, and ossification areas, with continued expression in the arterial tunica media.
Gene expression databases
Bgeei | ENSMUSG00000000957, Expressed in uterus and 293 other tissues |
ExpressionAtlasi | P53690, baseline and differential |
Genevisiblei | P53690, MM |
Interactioni
Subunit structurei
Interacts (via C-terminal cytoplasmic tail) with BST2.
Interacts with DLL1; inhibits DLL1-induced Notch signaling.
By similarityGO - Molecular functioni
- integrin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201446, 7 interactors |
IntActi | P53690, 1 interactor |
MINTi | P53690 |
STRINGi | 10090.ENSMUSP00000087119 |
Miscellaneous databases
RNActi | P53690, protein |
Structurei
3D structure databases
BMRBi | P53690 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 316 – 364 | Hemopexin 1Add BLAST | 49 | |
Repeati | 365 – 410 | Hemopexin 2Add BLAST | 46 | |
Repeati | 412 – 460 | Hemopexin 3Add BLAST | 49 | |
Repeati | 461 – 508 | Hemopexin 4Add BLAST | 48 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 91 – 98 | Cysteine switchBy similarity | 8 |
Domaini
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Sequence similaritiesi
Belongs to the peptidase M10A family.Curated
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1565, Eukaryota |
GeneTreei | ENSGT00940000157808 |
HOGENOMi | CLU_015489_8_1_1 |
InParanoidi | P53690 |
OMAi | LPTKMPP |
OrthoDBi | 1075463at2759 |
TreeFami | TF352396 |
Family and domain databases
CDDi | cd00094, HX, 1 hit cd04278, ZnMc_MMP, 1 hit |
Gene3Di | 1.10.101.10, 1 hit 2.110.10.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR000585, Hemopexin-like_dom IPR036375, Hemopexin-like_dom_sf IPR018487, Hemopexin-like_repeat IPR018486, Hemopexin_CS IPR033739, M10A_MMP IPR024079, MetalloPept_cat_dom_sf IPR001818, Pept_M10_metallopeptidase IPR021190, Pept_M10A IPR021805, Pept_M10A_metallopeptidase_C IPR021158, Pept_M10A_Zn_BS IPR006026, Peptidase_Metallo IPR002477, Peptidoglycan-bd-like IPR036365, PGBD-like_sf IPR036366, PGBDSf |
Pfami | View protein in Pfam PF11857, DUF3377, 1 hit PF00045, Hemopexin, 4 hits PF00413, Peptidase_M10, 1 hit PF01471, PG_binding_1, 1 hit |
PIRSFi | PIRSF001191, Peptidase_M10A_matrix, 1 hit |
PRINTSi | PR00138, MATRIXIN |
SMARTi | View protein in SMART SM00120, HX, 4 hits SM00235, ZnMc, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit SSF50923, SSF50923, 1 hit |
PROSITEi | View protein in PROSITE PS00546, CYSTEINE_SWITCH, 1 hit PS00024, HEMOPEXIN, 1 hit PS51642, HEMOPEXIN_2, 4 hits PS00142, ZINC_PROTEASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P53690-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSPAPRPSRS LLLPLLTLGT ALASLGWAQG SNFSPEAWLQ QYGYLPPGDL
60 70 80 90 100
RTHTQRSPQS LSAAIAAMQK FYGLQVTGKA DLATMMAMRR PRCGVPDKFG
110 120 130 140 150
TEIKANVRRK RYAIQGLKWQ HNEITFCIQN YTPKVGEYAT FEAIRKAFRV
160 170 180 190 200
WESATPLRFR EVPYAYIREG HEKQADIMIL FAEGFHGDST PFDGEGGFLA
210 220 230 240 250
HAYFPGPNIG GDTHFDSAEP WTVQNEDLNG NDIFLVAVHE LGHALGLEHS
260 270 280 290 300
NDPSAIMAPF YQWMDTENFV LPDDDRRGIQ QLYGSKSGSP TKMPPQPRTT
310 320 330 340 350
SRPSVPDKPK NPAYGPNICD GNFDTVAMLR GEMFVFKERW FWRVRNNQVM
360 370 380 390 400
DGYPMPIGQF WRGLPASINT AYERKDGKFV FFKGDKHWVF DEASLEPGYP
410 420 430 440 450
KHIKELGRGL PTDKIDAALF WMPNGKTYFF RGNKYYRFNE EFRAVDSEYP
460 470 480 490 500
KNIKVWEGIP ESPRGSFMGS DEVFTYFYKG NKYWKFNNQK LKVEPGYPKS
510 520 530 540 550
ALRDWMGCPS GGRPDEGTEE ETEVIIIEVD EEGSGAVSAA AVVLPVLLLL
560 570 580
LVLAVGLAVF FFRRHGTPKR LLYCQRSLLD KV
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A286YED2 | A0A286YED2_MOUSE | Matrix metalloproteinase-14 | Mmp14 | 558 | Annotation score: | ||
A0A0G2JE78 | A0A0G2JE78_MOUSE | Matrix metalloproteinase-14 | Mmp14 | 43 | Annotation score: | ||
A0A0G2JF39 | A0A0G2JF39_MOUSE | Matrix metalloproteinase-14 | Mmp14 | 11 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 133 | P → S in AAB86602 (PubMed:9325265).Curated | 1 | |
Sequence conflicti | 255 | A → D in CAA58520 (PubMed:7708715).Curated | 1 | |
Sequence conflicti | 258 | A → S in CAA58520 (PubMed:7708715).Curated | 1 | |
Sequence conflicti | 258 | A → S in AAB51753 (PubMed:9648071).Curated | 1 | |
Sequence conflicti | 341 | F → L in CAA58520 (PubMed:7708715).Curated | 1 | |
Sequence conflicti | 346 | N → P in AAB86602 (PubMed:9325265).Curated | 1 | |
Sequence conflicti | 378 | K → T in CAA58520 (PubMed:7708715).Curated | 1 | |
Sequence conflicti | 390 – 391 | FD → CV in CAA58520 (PubMed:7708715).Curated | 2 | |
Sequence conflicti | 400 – 401 | PK → AN in CAA58520 (PubMed:7708715).Curated | 2 | |
Sequence conflicti | 407 | G → V in CAA58520 (PubMed:7708715).Curated | 1 | |
Sequence conflicti | 412 | T → S in CAA58520 (PubMed:7708715).Curated | 1 | |
Sequence conflicti | 417 | A → T in CAA58520 (PubMed:7708715).Curated | 1 | |
Sequence conflicti | 512 | G → R in AAB51753 (PubMed:9648071).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83536 mRNA Translation: CAA58520.2 AF022432 , AF022424, AF022425, AF022426, AF022427, AF022428, AF022429, AF022430, AF022431 Genomic DNA Translation: AAB86602.1 U54984 mRNA Translation: AAB51753.1 DQ249870 mRNA Translation: ABB45784.1 AK088476 mRNA Translation: BAC40377.1 AK138611 mRNA Translation: BAE23719.1 AK149907 mRNA Translation: BAE29158.1 AK149988 mRNA Translation: BAE29216.1 AK165014 mRNA Translation: BAE38000.1 CH466535 Genomic DNA Translation: EDL36348.1 |
CCDSi | CCDS36922.1 |
PIRi | I48673 |
RefSeqi | NP_032634.3, NM_008608.4 |
Genome annotation databases
Ensembli | ENSMUST00000089688; ENSMUSP00000087119; ENSMUSG00000000957 |
GeneIDi | 17387 |
KEGGi | mmu:17387 |
UCSCi | uc007twc.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83536 mRNA Translation: CAA58520.2 AF022432 , AF022424, AF022425, AF022426, AF022427, AF022428, AF022429, AF022430, AF022431 Genomic DNA Translation: AAB86602.1 U54984 mRNA Translation: AAB51753.1 DQ249870 mRNA Translation: ABB45784.1 AK088476 mRNA Translation: BAC40377.1 AK138611 mRNA Translation: BAE23719.1 AK149907 mRNA Translation: BAE29158.1 AK149988 mRNA Translation: BAE29216.1 AK165014 mRNA Translation: BAE38000.1 CH466535 Genomic DNA Translation: EDL36348.1 |
CCDSi | CCDS36922.1 |
PIRi | I48673 |
RefSeqi | NP_032634.3, NM_008608.4 |
3D structure databases
BMRBi | P53690 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 201446, 7 interactors |
IntActi | P53690, 1 interactor |
MINTi | P53690 |
STRINGi | 10090.ENSMUSP00000087119 |
Chemistry databases
ChEMBLi | CHEMBL4295783 |
Protein family/group databases
MEROPSi | M10.014 |
PTM databases
iPTMneti | P53690 |
PhosphoSitePlusi | P53690 |
SwissPalmi | P53690 |
Proteomic databases
MaxQBi | P53690 |
PaxDbi | P53690 |
PeptideAtlasi | P53690 |
PRIDEi | P53690 |
Protocols and materials databases
Antibodypediai | 4088, 1006 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000089688; ENSMUSP00000087119; ENSMUSG00000000957 |
GeneIDi | 17387 |
KEGGi | mmu:17387 |
UCSCi | uc007twc.2, mouse |
Organism-specific databases
CTDi | 4323 |
MGIi | MGI:101900, Mmp14 |
Phylogenomic databases
eggNOGi | KOG1565, Eukaryota |
GeneTreei | ENSGT00940000157808 |
HOGENOMi | CLU_015489_8_1_1 |
InParanoidi | P53690 |
OMAi | LPTKMPP |
OrthoDBi | 1075463at2759 |
TreeFami | TF352396 |
Enzyme and pathway databases
Reactomei | R-MMU-1442490, Collagen degradation R-MMU-1474228, Degradation of the extracellular matrix R-MMU-1592389, Activation of Matrix Metalloproteinases |
Miscellaneous databases
BioGRID-ORCSi | 17387, 0 hits in 18 CRISPR screens |
ChiTaRSi | Mmp14, mouse |
PROi | PR:P53690 |
RNActi | P53690, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000000957, Expressed in uterus and 293 other tissues |
ExpressionAtlasi | P53690, baseline and differential |
Genevisiblei | P53690, MM |
Family and domain databases
CDDi | cd00094, HX, 1 hit cd04278, ZnMc_MMP, 1 hit |
Gene3Di | 1.10.101.10, 1 hit 2.110.10.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR000585, Hemopexin-like_dom IPR036375, Hemopexin-like_dom_sf IPR018487, Hemopexin-like_repeat IPR018486, Hemopexin_CS IPR033739, M10A_MMP IPR024079, MetalloPept_cat_dom_sf IPR001818, Pept_M10_metallopeptidase IPR021190, Pept_M10A IPR021805, Pept_M10A_metallopeptidase_C IPR021158, Pept_M10A_Zn_BS IPR006026, Peptidase_Metallo IPR002477, Peptidoglycan-bd-like IPR036365, PGBD-like_sf IPR036366, PGBDSf |
Pfami | View protein in Pfam PF11857, DUF3377, 1 hit PF00045, Hemopexin, 4 hits PF00413, Peptidase_M10, 1 hit PF01471, PG_binding_1, 1 hit |
PIRSFi | PIRSF001191, Peptidase_M10A_matrix, 1 hit |
PRINTSi | PR00138, MATRIXIN |
SMARTi | View protein in SMART SM00120, HX, 4 hits SM00235, ZnMc, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit SSF50923, SSF50923, 1 hit |
PROSITEi | View protein in PROSITE PS00546, CYSTEINE_SWITCH, 1 hit PS00024, HEMOPEXIN, 1 hit PS51642, HEMOPEXIN_2, 4 hits PS00142, ZINC_PROTEASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MMP14_MOUSE | |
Accessioni | P53690Primary (citable) accession number: P53690 Secondary accession number(s): O08645, O35369, Q8BTX2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 189 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries