UniProtKB - P53670 (LIMK2_RAT)
LIM domain kinase 2
Limk2
Functioni
Catalytic activityi
- EC:2.7.11.1By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.7.11.1By similarityThis reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 360 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 451 | By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 337 – 345 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- protein kinase activity Source: RGD
- protein serine/threonine kinase activity Source: RGD
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
GO - Biological processi
- actin cytoskeleton organization Source: GO_Central
- astral microtubule organization Source: UniProtKB
- cornea development in camera-type eye Source: RGD
- establishment of vesicle localization Source: UniProtKB
- head development Source: RGD
- negative regulation of cilium assembly Source: UniProtKB
- positive regulation of protein localization to nucleus Source: UniProtKB
- positive regulation of protein phosphorylation Source: UniProtKB
- protein phosphorylation Source: RGD
- spermatid development Source: RGD
- spermatogenesis Source: RGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 2.7.10.2, 5301 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Limk2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 62056, Limk2 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Cytoplasm By similarity
- perinuclear region By similarity
Cytoskeleton
- spindle By similarity
- centrosome By similarity
Cytoskeleton
- centrosome Source: UniProtKB
- mitotic spindle Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Golgi apparatus
- cis-Golgi network Source: RGD
Nucleus
- nucleus Source: RGD
Other locations
- cytoplasm Source: RGD
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075811 | 1 – 638 | LIM domain kinase 2Add BLAST | 638 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 210 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 293 | PhosphoserineBy similarity | 1 | |
Modified residuei | 298 | PhosphoserineBy similarity | 1 | |
Modified residuei | 505 | Phosphothreonine; by ROCK1 and CDC42BPBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P53670 |
PRIDEi | P53670 |
PTM databases
iPTMneti | P53670 |
PhosphoSitePlusi | P53670 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000019000, Expressed in brain and 22 other tissues |
ExpressionAtlasi | P53670, baseline and differential |
Genevisiblei | P53670, RN |
Interactioni
Subunit structurei
Binds ROCK1 and MARF1 (By similarity).
Interacts with NISCH (By similarity).
By similarityProtein-protein interaction databases
IntActi | P53670, 1 interactor |
STRINGi | 10116.ENSRNOP00000026032 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 63 | LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST | 52 | |
Domaini | 72 – 124 | LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 152 – 239 | PDZPROSITE-ProRule annotationAdd BLAST | 88 | |
Domaini | 331 – 608 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 278 |
Sequence similaritiesi
Keywords - Domaini
LIM domain, RepeatPhylogenomic databases
eggNOGi | KOG1187, Eukaryota |
GeneTreei | ENSGT00940000159133 |
HOGENOMi | CLU_110071_0_0_1 |
InParanoidi | P53670 |
OMAi | NCYCAGC |
OrthoDBi | 988822at2759 |
PhylomeDBi | P53670 |
TreeFami | TF318014 |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR001478, PDZ IPR036034, PDZ_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR001781, Znf_LIM |
Pfami | View protein in Pfam PF00412, LIM, 2 hits PF00595, PDZ, 1 hit PF07714, PK_Tyr_Ser-Thr, 1 hit |
SMARTi | View protein in SMART SM00132, LIM, 2 hits SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00478, LIM_DOMAIN_1, 2 hits PS50023, LIM_DOMAIN_2, 2 hits PS50106, PDZ, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAALAGEEAW RCRGCGNYVP LSQRLYRTAN EAWHSSCFRC SECQESLTNW
60 70 80 90 100
YYEKDGKLYC HKDYWAKFGE FCHGCSLLMT GPAMVAGEFK YHPECFACMS
110 120 130 140 150
CKVIIEDGDA YALVQHATLY CGKCHNEVVL APMFERLSTE SVQDQLPYSV
160 170 180 190 200
TLISMPATTE CRRGFSVSVE SASSNYATTV QVKEVNRMHI SPNNRNAIHP
210 220 230 240 250
GDRILEINGT PVRTLRVEEV EDAINQTSQT LQLLIEHDPV PQRLDQLRLD
260 270 280 290 300
TRLSPHMQSS GHTLMLSTLD AKENQEGTLR RRSLRRSNSI SKSPGPSSPK
310 320 330 340 350
EPLLLSRDIS RSESLRCSSS YSQQIFRPCD LIHGEVLGKG FFGQAIKVTH
360 370 380 390 400
KATGKVMVMK ELIRCDEETQ KTFLTEVKVM RSLDHPNVLK FIGVLYKDKK
410 420 430 440 450
LNLLTEYIEG GTLKDFLRNV DPFPWQQKVR FAKGIASGMA YLHSMCIIHR
460 470 480 490 500
DLNSHNCLIK LDKTVVVADF GLSRLIVEER KRPPVEKAAT KKRTLRKSDR
510 520 530 540 550
KKRYTVVGNP YWMAPEMLNG KSYDETVDVF SFGIVLCEII GQVYADPDCL
560 570 580 590 600
PRTLDFGLNV KLFWEKFVPT DCPPAFFPLA AICCKLEPES RPAFSKLEDS
610 620 630
FEALSLFLGE LAIPLPAELE ELDHTVSMEY GLTRDSPP
The sequence of this isoform differs from the canonical sequence as follows:
1-37: MAALAGEEAWRCRGCGNYVPLSQRLYRTANEAWHSSC → MGSYLSVPAYFTSRDP
The sequence of this isoform differs from the canonical sequence as follows:
1-37: MAALAGEEAWRCRGCGNYVPLSQRLYRTANEAWHSSC → MGSYLSVPAYFTSRDP
348-371: VTHKATGKVMVMKELIRCDEETQK → SWEGGSGDSQSHRQSDGHEGVNSL
372-638: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
1-37: MAALAGEEAWRCRGCGNYVPLSQRLYRTANEAWHSSC → MGSYLSVPAYFTSRDP
185-638: Missing.
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003128 | 1 – 37 | MAALA…WHSSC → MGSYLSVPAYFTSRDP in isoform LIMK2B, isoform LIMK2C and isoform LIMK2D. 1 PublicationAdd BLAST | 37 | |
Alternative sequenceiVSP_003131 | 185 – 638 | Missing in isoform LIMK2D. 1 PublicationAdd BLAST | 454 | |
Alternative sequenceiVSP_003129 | 348 – 371 | VTHKA…EETQK → SWEGGSGDSQSHRQSDGHEG VNSL in isoform LIMK2C. 1 PublicationAdd BLAST | 24 | |
Alternative sequenceiVSP_003130 | 372 – 638 | Missing in isoform LIMK2C. 1 PublicationAdd BLAST | 267 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D31874 mRNA Translation: BAA06673.1 D31875 mRNA Translation: BAA06674.1 D31876 mRNA Translation: BAA06675.1 D31877 mRNA Translation: BAA06676.1 |
PIRi | I78846 I78847 I78848 |
RefSeqi | NP_077049.2, NM_024135.2 [P53670-1] XP_006251298.1, XM_006251236.3 [P53670-2] |
Genome annotation databases
Ensembli | ENSRNOT00000026032; ENSRNOP00000026032; ENSRNOG00000019000 [P53670-1] ENSRNOT00000050005; ENSRNOP00000044951; ENSRNOG00000019000 [P53670-2] ENSRNOT00000057673; ENSRNOP00000054482; ENSRNOG00000019000 [P53670-4] |
GeneIDi | 29524 |
KEGGi | rno:29524 |
UCSCi | RGD:62056, rat [P53670-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D31874 mRNA Translation: BAA06673.1 D31875 mRNA Translation: BAA06674.1 D31876 mRNA Translation: BAA06675.1 D31877 mRNA Translation: BAA06676.1 |
PIRi | I78846 I78847 I78848 |
RefSeqi | NP_077049.2, NM_024135.2 [P53670-1] XP_006251298.1, XM_006251236.3 [P53670-2] |
3D structure databases
SMRi | P53670 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P53670, 1 interactor |
STRINGi | 10116.ENSRNOP00000026032 |
PTM databases
iPTMneti | P53670 |
PhosphoSitePlusi | P53670 |
Proteomic databases
PaxDbi | P53670 |
PRIDEi | P53670 |
Genome annotation databases
Ensembli | ENSRNOT00000026032; ENSRNOP00000026032; ENSRNOG00000019000 [P53670-1] ENSRNOT00000050005; ENSRNOP00000044951; ENSRNOG00000019000 [P53670-2] ENSRNOT00000057673; ENSRNOP00000054482; ENSRNOG00000019000 [P53670-4] |
GeneIDi | 29524 |
KEGGi | rno:29524 |
UCSCi | RGD:62056, rat [P53670-1] |
Organism-specific databases
CTDi | 3985 |
RGDi | 62056, Limk2 |
Phylogenomic databases
eggNOGi | KOG1187, Eukaryota |
GeneTreei | ENSGT00940000159133 |
HOGENOMi | CLU_110071_0_0_1 |
InParanoidi | P53670 |
OMAi | NCYCAGC |
OrthoDBi | 988822at2759 |
PhylomeDBi | P53670 |
TreeFami | TF318014 |
Enzyme and pathway databases
BRENDAi | 2.7.10.2, 5301 |
Miscellaneous databases
PROi | PR:P53670 |
Gene expression databases
Bgeei | ENSRNOG00000019000, Expressed in brain and 22 other tissues |
ExpressionAtlasi | P53670, baseline and differential |
Genevisiblei | P53670, RN |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR001478, PDZ IPR036034, PDZ_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR001781, Znf_LIM |
Pfami | View protein in Pfam PF00412, LIM, 2 hits PF00595, PDZ, 1 hit PF07714, PK_Tyr_Ser-Thr, 1 hit |
SMARTi | View protein in SMART SM00132, LIM, 2 hits SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00478, LIM_DOMAIN_1, 2 hits PS50023, LIM_DOMAIN_2, 2 hits PS50106, PDZ, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LIMK2_RAT | |
Accessioni | P53670Primary (citable) accession number: P53670 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families