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UniProtKB - P53657 (KPYR_MOUSE)

Protein

Pyruvate kinase PKLR

Gene

Pklr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a key role in glycolysis.By similarity

Miscellaneous

There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Activity regulationi

Allosterically activated by fructose 1,6-bisphosphate.By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gm3839), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gm10358)
  2. Phosphoglycerate kinase 1 (Pgk1), Phosphoglycerate kinase 2 (Pgk2), Phosphoglycerate kinase (Pgk1), Phosphoglycerate kinase (Pgk1)
  3. no protein annotated in this organism
  4. Gamma-enolase (Eno2), Enolase 4 (Eno4), Alpha-enolase (Eno1), Beta-enolase (Eno3)
  5. Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase PKM (Pkm), Pyruvate kinase PKLR (Pklr), Pyruvate kinase (Pklr)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116SubstrateBy similarity1
Metal bindingi118PotassiumBy similarity1
Metal bindingi120PotassiumBy similarity1
Metal bindingi156PotassiumBy similarity1
Metal bindingi157Potassium; via carbonyl oxygenBy similarity1
Sitei313Transition state stabilizerBy similarity1
Metal bindingi315MagnesiumBy similarity1
Binding sitei338Substrate; via amide nitrogenBy similarity1
Metal bindingi339MagnesiumBy similarity1
Binding sitei339Substrate; via amide nitrogenBy similarity1
Binding sitei371SubstrateBy similarity1
Binding sitei525Allosteric activatorBy similarity1
Binding sitei532Allosteric activatorBy similarity1

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

SABIO-RKiP53657
UniPathwayi
UPA00109;UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase PKLR (EC:2.7.1.40)
Alternative name(s):
L-PK
Pyruvate kinase isozymes L/R
Gene namesi
Name:Pklr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97604 Pklr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120951 – 574Pyruvate kinase PKLRAdd BLAST574

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2PhosphoserineBy similarity1
Modified residuei19PhosphoserineBy similarity1
Modified residuei26PhosphoserineBy similarity1
Modified residuei43Phosphoserine; by PKABy similarity1
Modified residuei292PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP53657
PeptideAtlasiP53657
PRIDEiP53657

PTM databases

iPTMnetiP53657
PhosphoSitePlusiP53657

Expressioni

Gene expression databases

CleanExiMM_PKLR

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

ComplexPortaliCPX-3097 PKL pyruvate kinase complex [P53657-2]
CPX-3098 PKR pyruvate kinase complex [P53657-1]
IntActiP53657, 5 interactors
MINTiP53657
STRINGi10090.ENSMUSP00000035417

Structurei

3D structure databases

ProteinModelPortaliP53657
SMRiP53657
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni475 – 480Allosteric activator bindingBy similarity6
Regioni559 – 564Allosteric activator bindingBy similarity6

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2323 Eukaryota
COG0469 LUCA
HOGENOMiHOG000021559
HOVERGENiHBG000941
InParanoidiP53657
PhylomeDBiP53657

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 2 hits
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform R-type (identifier: P53657-1) [UniParc]FASTAAdd to basket
Also known as: PKR

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSVQENELPQ QLWPWIFKSQ KDLAKSALSG APGGPAGYLR RASVAQLTQE 
        60         70         80         90        100
LGTAFFQQQQ LPAAMADTFL EHLCLLDIDS EPVAARSTSI IATIGPASRS 
       110        120        130        140        150
VDRLKEMIKA GMNIARLNFS HGSHEYHAES IANIREAAES FATSPLSYRP 
       160        170        180        190        200
VAIALDTKGP EIRTGVLQGG PESEVEIVKG SQVLVTVDPK FRTRGDAKTV 
       210        220        230        240        250
WVDYHNITQV VAVGGRIYID DGLISLVVRK IGPEGLVTEV EHGGFLGNRK 
       260        270        280        290        300
GVNLPNAEVD LPGLSEQDLL DLRFGVEHYV DIIFASFVRK ASDVVAVRDA 
       310        320        330        340        350
LGPEGRGIKI ISKIENHEGV KKFDEILEVS DGIMMARGDL GIEIPAEKVF 
       360        370        380        390        400
LAQKMMIGRC NLAGKPVVCA TQMLESMITK ARPTRAETSD VANAVLDGAD 
       410        420        430        440        450
CIMLSGETAK GSFPVEAVKM QHAIAREAEA AVYHRQLFEE LRRAAPLSRD 
       460        470        480        490        500
PTEVTAIGAV EASFKCCAAA IIVLTKTGRS AQLLSRYRPR AAVIAVTRSA 
       510        520        530        540        550
QAARQVHLSR GVFPLLYREP PEAVWADDVD RRVQFGIESG KLRGFLRVGD 
       560        570 
LVIVVTGWRP GSGYTNIMRV LTIS
Length:574
Mass (Da):62,309
Last modified:October 1, 1996 - v1
Checksum:i6C4689DE954E6166
GO
Isoform L-type (identifier: P53657-2)
Also known as: PKL
Sequence is not available
Length:–
Mass (Da):–

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3X925G3X925_MOUSE
Pyruvate kinase
Pklr mCG_17567
574Annotation score:
E9Q509E9Q509_MOUSE
Pyruvate kinase
Pklr
543Annotation score:
D3Z2C4D3Z2C4_MOUSE
Pyruvate kinase PKLR
Pklr
72Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79731 mRNA Translation: AAB35435.1
D63764 mRNA Translation: BAA23642.1
CCDSiCCDS17489.1 [P53657-1]
UniGeneiMm.383180

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79731 mRNA Translation: AAB35435.1
D63764 mRNA Translation: BAA23642.1
CCDSiCCDS17489.1 [P53657-1]
UniGeneiMm.383180

3D structure databases

ProteinModelPortaliP53657
SMRiP53657
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-3097 PKL pyruvate kinase complex [P53657-2]
CPX-3098 PKR pyruvate kinase complex [P53657-1]
IntActiP53657, 5 interactors
MINTiP53657
STRINGi10090.ENSMUSP00000035417

PTM databases

iPTMnetiP53657
PhosphoSitePlusiP53657

Proteomic databases

PaxDbiP53657
PeptideAtlasiP53657
PRIDEiP53657

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:97604 Pklr

Phylogenomic databases

eggNOGiKOG2323 Eukaryota
COG0469 LUCA
HOGENOMiHOG000021559
HOVERGENiHBG000941
InParanoidiP53657
PhylomeDBiP53657

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00188

SABIO-RKiP53657

Miscellaneous databases

ChiTaRSiPklr mouse
PROiPR:P53657
SOURCEiSearch...

Gene expression databases

CleanExiMM_PKLR

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 2 hits
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKPYR_MOUSE
AccessioniPrimary (citable) accession number: P53657
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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