UniProtKB - P53657 (KPYR_MOUSE)
Protein
Pyruvate kinase PKLR
Gene
Pklr
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a key role in glycolysis.By similarity
Miscellaneous
There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.
Catalytic activityi
- EC:2.7.1.40
Cofactori
Protein has several cofactor binding sites:Activity regulationi
Allosterically activated by fructose 1,6-bisphosphate.By similarity
: glycolysis Pathwayi
This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gm10358), Glyceraldehyde-3-phosphate dehydrogenase (Gm3839), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Gapdhs)
- Phosphoglycerate kinase 1 (Pgk1), Phosphoglycerate kinase (Pgk1), Phosphoglycerate kinase (Pgk1), Phosphoglycerate kinase 2 (Pgk2)
- no protein annotated in this organism
- 2-phospho-D-glycerate hydro-lyase (Eno2), 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydro-lyase (Eno3), 2-phospho-D-glycerate hydro-lyase (Eno4), 2-phospho-D-glycerate hydro-lyase (Eno2), 2-phospho-D-glycerate hydro-lyase (Eno4), 2-phospho-D-glycerate hydro-lyase (Eno2), 2-phospho-D-glycerate hydro-lyase (Eno1), 2-phospho-D-glycerate hydro-lyase (EG433182), 2-phospho-D-glycerate hydro-lyase (Eno2), 2-phospho-D-glycerate hydro-lyase (Eno4), Gamma-enolase (Eno2), 2-phospho-D-glycerate hydro-lyase (Eno3), Beta-enolase (Eno3), Alpha-enolase (Eno1), 2-phospho-D-glycerate hydro-lyase (Eno2), 2-phospho-D-glycerate hydro-lyase (Eno2), 2-phospho-D-glycerate hydro-lyase (Eno3), 2-phospho-D-glycerate hydro-lyase (Eno1), Enolase 4 (Eno4)
- Pyruvate kinase (Pkm), Pyruvate kinase (Pkm), Pyruvate kinase (Pkm), Pyruvate kinase (Pklr), Pyruvate kinase (Pkm), Pyruvate kinase (Pklr), Pyruvate kinase (Pkm), Pyruvate kinase PKLR (Pklr), Pyruvate kinase (Pkm), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase PKM (Pkm)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 116 | SubstrateBy similarity | 1 | |
Metal bindingi | 118 | PotassiumBy similarity | 1 | |
Metal bindingi | 120 | PotassiumBy similarity | 1 | |
Metal bindingi | 156 | PotassiumBy similarity | 1 | |
Metal bindingi | 157 | Potassium; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 163 | ATPBy similarity | 1 | |
Binding sitei | 250 | ATPBy similarity | 1 | |
Sitei | 313 | Transition state stabilizerBy similarity | 1 | |
Metal bindingi | 315 | MagnesiumBy similarity | 1 | |
Binding sitei | 338 | Substrate; via amide nitrogenBy similarity | 1 | |
Metal bindingi | 339 | MagnesiumBy similarity | 1 | |
Binding sitei | 339 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 371 | SubstrateBy similarity | 1 | |
Binding sitei | 525 | Allosteric activatorBy similarity | 1 | |
Binding sitei | 532 | Allosteric activatorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 118 – 121 | ATPBy similarity | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- kinase activity Source: UniProtKB-KW
- magnesium ion binding Source: InterPro
- potassium ion binding Source: InterPro
- pyruvate kinase activity Source: MGI
GO - Biological processi
- ATP biosynthetic process Source: MGI
- carbohydrate metabolic process Source: MGI
- cellular response to insulin stimulus Source: MGI
- endocrine pancreas development Source: Reactome
- glycolytic process Source: MGI
- pyruvate biosynthetic process Source: MGI
- response to ATP Source: MGI
- response to cAMP Source: MGI
- response to heat Source: MGI
- response to lithium ion Source: MGI
- response to other organism Source: MGI
Keywordsi
Molecular function | Allosteric enzyme, Kinase, Transferase |
Biological process | Glycolysis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate |
Enzyme and pathway databases
Reactomei | R-MMU-70171, Glycolysis [P53657-2] |
SABIO-RKi | P53657 |
UniPathwayi | UPA00109;UER00188 |
Names & Taxonomyi
Protein namesi | Recommended name: Pyruvate kinase PKLR (EC:2.7.1.40)Alternative name(s): L-PK Pyruvate kinase isozymes L/R |
Gene namesi | Name:Pklr |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97604, Pklr |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: MGI
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000112095 | 1 – 574 | Pyruvate kinase PKLRAdd BLAST | 574 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | PhosphoserineBy similarity | 1 | |
Modified residuei | 19 | PhosphoserineBy similarity | 1 | |
Modified residuei | 26 | PhosphoserineBy similarity | 1 | |
Modified residuei | 43 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 292 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P53657 |
PaxDbi | P53657 |
PeptideAtlasi | P53657 |
PRIDEi | P53657 |
PTM databases
iPTMneti | P53657 |
PhosphoSitePlusi | P53657 |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
ComplexPortali | CPX-3097, PKL pyruvate kinase complex [P53657-2] CPX-3098, PKR pyruvate kinase complex [P53657-1] |
IntActi | P53657, 5 interactors |
MINTi | P53657 |
STRINGi | 10090.ENSMUSP00000035417 |
Miscellaneous databases
RNActi | P53657, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 475 – 480 | Allosteric activator bindingBy similarity | 6 | |
Regioni | 559 – 564 | Allosteric activator bindingBy similarity | 6 |
Sequence similaritiesi
Belongs to the pyruvate kinase family.Curated
Phylogenomic databases
eggNOGi | KOG2323, Eukaryota |
InParanoidi | P53657 |
PhylomeDBi | P53657 |
Family and domain databases
CDDi | cd00288, Pyruvate_Kinase, 1 hit |
Gene3Di | 2.40.33.10, 1 hit 3.20.20.60, 1 hit 3.40.1380.20, 1 hit |
InterProi | View protein in InterPro IPR001697, Pyr_Knase IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf IPR011037, Pyrv_Knase-like_insert_dom_sf IPR018209, Pyrv_Knase_AS IPR015793, Pyrv_Knase_brl IPR015795, Pyrv_Knase_C IPR036918, Pyrv_Knase_C_sf IPR015806, Pyrv_Knase_insert_dom_sf |
PANTHERi | PTHR11817, PTHR11817, 1 hit |
Pfami | View protein in Pfam PF00224, PK, 1 hit PF02887, PK_C, 1 hit |
PRINTSi | PR01050, PYRUVTKNASE |
SUPFAMi | SSF50800, SSF50800, 1 hit SSF51621, SSF51621, 1 hit SSF52935, SSF52935, 1 hit |
TIGRFAMsi | TIGR01064, pyruv_kin, 1 hit |
PROSITEi | View protein in PROSITE PS00110, PYRUVATE_KINASE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform R-type (identifier: P53657-1) [UniParc]FASTAAdd to basket
Also known as: PKR
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSVQENELPQ QLWPWIFKSQ KDLAKSALSG APGGPAGYLR RASVAQLTQE
60 70 80 90 100
LGTAFFQQQQ LPAAMADTFL EHLCLLDIDS EPVAARSTSI IATIGPASRS
110 120 130 140 150
VDRLKEMIKA GMNIARLNFS HGSHEYHAES IANIREAAES FATSPLSYRP
160 170 180 190 200
VAIALDTKGP EIRTGVLQGG PESEVEIVKG SQVLVTVDPK FRTRGDAKTV
210 220 230 240 250
WVDYHNITQV VAVGGRIYID DGLISLVVRK IGPEGLVTEV EHGGFLGNRK
260 270 280 290 300
GVNLPNAEVD LPGLSEQDLL DLRFGVEHYV DIIFASFVRK ASDVVAVRDA
310 320 330 340 350
LGPEGRGIKI ISKIENHEGV KKFDEILEVS DGIMMARGDL GIEIPAEKVF
360 370 380 390 400
LAQKMMIGRC NLAGKPVVCA TQMLESMITK ARPTRAETSD VANAVLDGAD
410 420 430 440 450
CIMLSGETAK GSFPVEAVKM QHAIAREAEA AVYHRQLFEE LRRAAPLSRD
460 470 480 490 500
PTEVTAIGAV EASFKCCAAA IIVLTKTGRS AQLLSRYRPR AAVIAVTRSA
510 520 530 540 550
QAARQVHLSR GVFPLLYREP PEAVWADDVD RRVQFGIESG KLRGFLRVGD
560 570
LVIVVTGWRP GSGYTNIMRV LTIS
Length:–
Mass (Da):–
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3X925 | G3X925_MOUSE | Pyruvate kinase | Pklr | 574 | Annotation score: | ||
E9Q509 | E9Q509_MOUSE | Pyruvate kinase | Pklr | 543 | Annotation score: | ||
D3Z2C4 | D3Z2C4_MOUSE | Pyruvate kinase | Pklr | 72 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S79731 mRNA Translation: AAB35435.1 D63764 mRNA Translation: BAA23642.1 |
CCDSi | CCDS17489.1 [P53657-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S79731 mRNA Translation: AAB35435.1 D63764 mRNA Translation: BAA23642.1 |
CCDSi | CCDS17489.1 [P53657-1] |
3D structure databases
SMRi | P53657 |
ModBasei | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-3097, PKL pyruvate kinase complex [P53657-2] CPX-3098, PKR pyruvate kinase complex [P53657-1] |
IntActi | P53657, 5 interactors |
MINTi | P53657 |
STRINGi | 10090.ENSMUSP00000035417 |
PTM databases
iPTMneti | P53657 |
PhosphoSitePlusi | P53657 |
Proteomic databases
jPOSTi | P53657 |
PaxDbi | P53657 |
PeptideAtlasi | P53657 |
PRIDEi | P53657 |
Organism-specific databases
MGIi | MGI:97604, Pklr |
Phylogenomic databases
eggNOGi | KOG2323, Eukaryota |
InParanoidi | P53657 |
PhylomeDBi | P53657 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00188 |
Reactomei | R-MMU-70171, Glycolysis [P53657-2] |
SABIO-RKi | P53657 |
Miscellaneous databases
ChiTaRSi | Pklr, mouse |
PROi | PR:P53657 |
RNActi | P53657, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd00288, Pyruvate_Kinase, 1 hit |
Gene3Di | 2.40.33.10, 1 hit 3.20.20.60, 1 hit 3.40.1380.20, 1 hit |
InterProi | View protein in InterPro IPR001697, Pyr_Knase IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf IPR011037, Pyrv_Knase-like_insert_dom_sf IPR018209, Pyrv_Knase_AS IPR015793, Pyrv_Knase_brl IPR015795, Pyrv_Knase_C IPR036918, Pyrv_Knase_C_sf IPR015806, Pyrv_Knase_insert_dom_sf |
PANTHERi | PTHR11817, PTHR11817, 1 hit |
Pfami | View protein in Pfam PF00224, PK, 1 hit PF02887, PK_C, 1 hit |
PRINTSi | PR01050, PYRUVTKNASE |
SUPFAMi | SSF50800, SSF50800, 1 hit SSF51621, SSF51621, 1 hit SSF52935, SSF52935, 1 hit |
TIGRFAMsi | TIGR01064, pyruv_kin, 1 hit |
PROSITEi | View protein in PROSITE PS00110, PYRUVATE_KINASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KPYR_MOUSE | |
Accessioni | P53657Primary (citable) accession number: P53657 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families