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Protein

Poly(A) RNA polymerase protein 2

Gene

PAP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of the TRAMP complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates like cryptic transcripts generated by RNA polymerase II and III, or hypomethylated pre-tRNAi-Met. Polyadenylates RNA processing and degradation intermediates of snRNAs, snoRNAs and mRNAs that accumulate in strains lacking a functional exosome. TRF4 is also required for proper nuclear division in mitosis, DNA damage repair and sister chromatid cohesion. Involved in the regulation of histone mRNA levels. May mediate mitotic chromosome condensation.17 Publications

Miscellaneous

Present with 7550 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to have DNA polymerase activity.Curated

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi236Magnesium or manganese; catalyticBy similarity1
Metal bindingi238Magnesium or manganese; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processCell cycle, Cell division, Mitosis
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33512-MONOMER
BRENDAi4.2.99.B1 984

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase protein 2 (EC:2.7.7.-)
Alternative name(s):
DNA polymerase kappa
DNA polymerase sigma
Topoisomerase 1-related protein TRF4
Gene namesi
Name:PAP2
Synonyms:TRF4
Ordered Locus Names:YOL115W
ORF Names:HRC584, O0716
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL115W
SGDiS000005475 PAP2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi131 – 133EDE → AAA in TRF4-131; slow growth. 1 Publication3
Mutagenesisi140 – 142ERE → AAA in TRF4-140; slow growth. 1 Publication3
Mutagenesisi182 – 185EIKD → AIAA in TRF4-182; lethal. 1 Publication4
Mutagenesisi194R → A in TRF4-194; lethal; when associated with A-195; A-196 and A-198. 1
Mutagenesisi195 – 198EEIE → AIAA in TRF4-194; lethal. 4
Mutagenesisi217 – 219DAD → AAA in TRF4-217; slow growth. 1 Publication3
Mutagenesisi224 – 225GS → AA in TRF4-224; lethal. 1 Publication2
Mutagenesisi236 – 238DID → AIA in TRF4-236; lethal. 3 Publications3
Mutagenesisi275 – 277KAR → AAA in TRF4-275; temperature sensitive. 1 Publication3
Mutagenesisi282K → A in TRF4-282; lethal; when associated with A-285. 1
Mutagenesisi285E → A in TRF4-282; lethal; when associated with A-282. 1
Mutagenesisi309 – 310RE → AA in TRF4-309; lethal. 1 Publication2
Mutagenesisi332 – 333RR → AA in TRF4-332; temperature sensitive. 1 Publication2
Mutagenesisi378E → A in TRF4-378; lethal; when associated with A-381. 1
Mutagenesisi381E → A in TRF4-378; lethal; when associated with A-378. 1
Mutagenesisi425D → A in TRF4-425; lethal; when associated with A-428 and A-429. 1
Mutagenesisi428 – 429DE → AA in TRF4-425; lethal; when associated with A-425. 2
Mutagenesisi444 – 445KK → AA in TRF4-444; lethal. 1 Publication2
Mutagenesisi467 – 469KDR → AAA in TRF4-467; temperature sensitive. 1 Publication3
Mutagenesisi486 – 487RD → AA in TRF4-486; lethal. 2
Mutagenesisi490 – 492DER → AAA in TRF4-486; lethal. 3
Mutagenesisi502E → A in TRF4-502; lethal; when associated with A-504. 1
Mutagenesisi504E → A in TRF4-502; lethal; when associated with A-502. 1
Mutagenesisi508 – 510KKR → AAA in TRF4-508; lethal. 1 Publication3
Mutagenesisi559 – 560KR → AA in TRF4-559; slow growth. 1 Publication2
Mutagenesisi560R → A in TRF4-559; slow growth; when associated with A-559. 1
Mutagenesisi572 – 574EDD → AAA in TRF4-572; slow growth. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001203141 – 584Poly(A) RNA polymerase protein 2Add BLAST584

Proteomic databases

MaxQBiP53632
PaxDbiP53632
PRIDEiP53632

PTM databases

iPTMnetiP53632

Interactioni

Subunit structurei

Component of the TRAMP complex (also called TRF4 complex) composed of at least HUL4, MTR4, PAP2/TRF4 and either AIR1 or AIR2. Interacts with NOP53 and POL2. Interacts directly with AIR2.6 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi34285, 420 interactors
ComplexPortaliCPX-1678 TRAMP complex variant 1
CPX-1679 TRAMP complex variant 2
DIPiDIP-4214N
IntActiP53632, 40 interactors
MINTiP53632
STRINGi4932.YOL115W

Structurei

Secondary structure

1584
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP53632
SMRiP53632
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53632

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini371 – 431PAP-associatedAdd BLAST61

Sequence similaritiesi

Belongs to the DNA polymerase type-B-like family.Curated

Phylogenomic databases

GeneTreeiENSGT00400000022055
HOGENOMiHOG000246586
InParanoidiP53632
KOiK03514
OMAiRKQYEMR
OrthoDBiEOG092C144L

Family and domain databases

InterProiView protein in InterPro
IPR002058 PAP_assoc
IPR002934 Polymerase_NTP_transf_dom
PfamiView protein in Pfam
PF01909 NTP_transf_2, 1 hit
PF03828 PAP_assoc, 1 hit

Sequencei

Sequence statusi: Complete.

P53632-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGAKSVTASS SKKIKNRHNG KVKKSKKIKK VRKPQKSISL NDENEVEILP
60 70 80 90 100
SRNEQETNKL PKDHVTADGI LVLEHKSDDD EGFDVYDGHF DNPTDIPSTT
110 120 130 140 150
EESKTPSLAV HGDEKDLANN DDFISLSASS EDEQAEQEEE REKQELEIKK
160 170 180 190 200
EKQKEILNTD YPWILNHDHS KQKEISDWLT FEIKDFVAYI SPSREEIEIR
210 220 230 240 250
NQTISTIREA VKQLWPDADL HVFGSYSTDL YLPGSDIDCV VTSELGGKES
260 270 280 290 300
RNNLYSLASH LKKKNLATEV EVVAKARVPI IKFVEPHSGI HIDVSFERTN
310 320 330 340 350
GIEAAKLIRE WLDDTPGLRE LVLIVKQFLH ARRLNNVHTG GLGGFSIICL
360 370 380 390 400
VFSFLHMHPR IITNEIDPKD NLGVLLIEFF ELYGKNFGYD DVALGSSDGY
410 420 430 440 450
PVYFPKSTWS AIQPIKNPFS LAIQDPGDES NNISRGSFNI RDIKKAFAGA
460 470 480 490 500
FDLLTNRCFE LHSATFKDRL GKSILGNVIK YRGKARDFKD ERGLVLNKAI
510 520 530 540 550
IENENYHKKR SRIIHDEDFA EDTVTSTATA TTTDDDYEIT NPPAKKAKIE
560 570 580
EKPESEPAKR NSGETYITVS SEDDDEDGYN PYTL
Length:584
Mass (Da):66,031
Last modified:October 1, 1996 - v1
Checksum:i8A58B29E4BFDC022
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31355 Genomic DNA Translation: AAC49091.1
Z48149 Genomic DNA Translation: CAA88145.1
Z74857 Genomic DNA Translation: CAA99134.1
AY723865 Genomic DNA Translation: AAU09782.1
BK006948 Genomic DNA Translation: DAA10668.1
PIRiS51882
RefSeqiNP_014526.1, NM_001183369.1

Genome annotation databases

EnsemblFungiiYOL115W; YOL115W; YOL115W
GeneIDi854034
KEGGisce:YOL115W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31355 Genomic DNA Translation: AAC49091.1
Z48149 Genomic DNA Translation: CAA88145.1
Z74857 Genomic DNA Translation: CAA99134.1
AY723865 Genomic DNA Translation: AAU09782.1
BK006948 Genomic DNA Translation: DAA10668.1
PIRiS51882
RefSeqiNP_014526.1, NM_001183369.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MOWNMR-B573-584[»]
3NYBX-ray2.70A161-481[»]
4U4CX-ray2.40B111-160[»]
ProteinModelPortaliP53632
SMRiP53632
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34285, 420 interactors
ComplexPortaliCPX-1678 TRAMP complex variant 1
CPX-1679 TRAMP complex variant 2
DIPiDIP-4214N
IntActiP53632, 40 interactors
MINTiP53632
STRINGi4932.YOL115W

PTM databases

iPTMnetiP53632

Proteomic databases

MaxQBiP53632
PaxDbiP53632
PRIDEiP53632

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL115W; YOL115W; YOL115W
GeneIDi854034
KEGGisce:YOL115W

Organism-specific databases

EuPathDBiFungiDB:YOL115W
SGDiS000005475 PAP2

Phylogenomic databases

GeneTreeiENSGT00400000022055
HOGENOMiHOG000246586
InParanoidiP53632
KOiK03514
OMAiRKQYEMR
OrthoDBiEOG092C144L

Enzyme and pathway databases

BioCyciYEAST:G3O-33512-MONOMER
BRENDAi4.2.99.B1 984

Miscellaneous databases

EvolutionaryTraceiP53632
PROiPR:P53632

Family and domain databases

InterProiView protein in InterPro
IPR002058 PAP_assoc
IPR002934 Polymerase_NTP_transf_dom
PfamiView protein in Pfam
PF01909 NTP_transf_2, 1 hit
PF03828 PAP_assoc, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPAP2_YEAST
AccessioniPrimary (citable) accession number: P53632
Secondary accession number(s): D6W1V2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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Main funding by: National Institutes of Health

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