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UniProtKB - P53624 (MA1A1_DROME)
Protein
Mannosyl-oligosaccharide alpha-1,2-mannosidase IA
Gene
alpha-Man-Ia
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.
By similarityCatalytic activityi
- 4 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
- 3 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
Cofactori
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 529 | Proton donorBy similarity | 1 | |
Metal bindingi | 640 | CalciumBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: FlyBase
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- determination of adult lifespan Source: FlyBase
- encapsulation of foreign target Source: FlyBase
- N-glycan processing Source: GO_Central
- protein glycosylation Source: UniProtKB-UniPathway
- response to anesthetic Source: FlyBase
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-DME-964827, Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
Names & Taxonomyi
Protein namesi | Recommended name: Mannosyl-oligosaccharide alpha-1,2-mannosidase IAImported (EC:3.2.1.113By similarity)Alternative name(s): Man(9)-alpha-mannosidase Mannosidase-1 |
Gene namesi | ORF Names:CG32684Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0259170, alpha-Man-Ia |
VEuPathDBi | VectorBase:FBgn0259170 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Golgi apparatus
- Golgi membrane Source: GO_Central
Other locations
- endomembrane system Source: FlyBase
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 18 | CytoplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 19 – 39 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 40 – 667 | LumenalSequence analysisAdd BLAST | 628 |
Keywords - Cellular componenti
Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210316 | 1 – 667 | Mannosyl-oligosaccharide alpha-1,2-mannosidase IAAdd BLAST | 667 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 278 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 483 ↔ 515 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PRIDEi | P53624 |
PTM databases
GlyGeni | P53624, 1 site |
Expressioni
Tissue specificityi
Complex spatial distribution during embryogenesis, including expression in lobula plate giant neurons. Also expressed in adult wing and eyes.1 Publication
Developmental stagei
Expressed both maternally and zygotically during embryonic stages.1 Publication
Gene expression databases
Bgeei | FBgn0259170, Expressed in embryo and 36 other tissues |
ExpressionAtlasi | P53624, baseline and differential |
Genevisiblei | P53624, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 58390, 3 interactors |
IntActi | P53624, 2 interactors |
STRINGi | 7227.FBpp0288908 |
Chemistry databases
BindingDBi | P53624 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 154 – 192 | DisorderedSequence analysisAdd BLAST | 39 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 155 – 182 | Polar residuesSequence analysisAdd BLAST | 28 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 47 family.UniRule annotation
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2204, Eukaryota |
GeneTreei | ENSGT00940000167910 |
HOGENOMi | CLU_003818_3_2_1 |
InParanoidi | P53624 |
OMAi | LIKMYLY |
PhylomeDBi | P53624 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative promoter usage. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform HImported (identifier: P53624-1) [UniParc]FASTAAdd to basket
Also known as: A1 Publication, JImported, LImported, MImported, NImported, OImported
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MYRISPIGRK SNFHSREKCL IGLVLVTLCF LCFGGIFLLP DNFGSDRVLR
60 70 80 90 100
VYKHFRKAGP EIFIPAPPLA AHAPHRSEDP HFIGDRQRLE QKIRAELGDM
110 120 130 140 150
LDEPPAAGGG EPGQFQVLAQ QAQAPAPVAA LADQPLDQDE GHAAIPVLAA
160 170 180 190 200
PVQGDNAASQ ASSHPQSSAQ QHNQQQPQLP LGGGGNDQAP DTLDATLEER
210 220 230 240 250
RQKVKEMMEH AWHNYKLYAW GKNELRPLSQ RPHSASIFGS YDLGATIVDG
260 270 280 290 300
LDTLYIMGLE KEYREGRDWI ERKFSLDNIS AELSVFETNI RFVGGMLTLY
310 320 330 340 350
AFTGDPLYKE KAQHVADKLL PAFQTPTGIP YALVNTKTGV AKNYGWASGG
360 370 380 390 400
SSILSEFGTL HLEFAYLSDI TGNPLYRERV QTIRQVLKEI EKPKGLYPNF
410 420 430 440 450
LNPKTGKWGQ LHMSLGALGD SYYEYLLKAW LQSGQTDEEA REMFDEAMLA
460 470 480 490 500
ILDKMVRTSP GGLTYVSDLK FDRLEHKMDH LACFSGGLFA LGAATRQNDY
510 520 530 540 550
TDKYMEVGKG ITNTCHESYI RAPTQLGPEA FRFSEAVEAR ALRSQEKYYI
560 570 580 590 600
LRPETFESYF VLWRLTHDQK YRDWGWEAVL ALEKHCRTAH GYCGLRNVYQ
610 620 630 640 650
QEPQKDDVQQ SFFLAETLKY LYLLFSDDSV LPLDEWVFNT EAHPLPIKGA
660
NAYYRQAPVT LPVSNAS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ8IRL4 | Q8IRL4_DROME | alpha-1,2-Mannosidase | alpha-Man-Ia a-Man-Ia, alpha-Man-I, alpha-Man-l, CG17809, CG18799 | 684 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 21 | I → T in CAA57962 (PubMed:7729592).Curated | 1 | |
Sequence conflicti | 568 | D → E in CAA57962 (PubMed:7729592).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_057904 | 1 – 206 | MYRIS…QKVKE → MCPKTSKTTPLLLIGGICFV IVLVGITGITLINNINLSNI IRLNEKVASDSVSNNENQIK ELNYVNNHPRNVYLKLNASS RDDEDDEQMQKEQEQLELPK ISAVIGGSKPKVEDNQVKES SEVISPSTSTFSMRSSAGEL TSTSAPLSSIVSVTAPPMPF GGVKYNQSSGLIDYEKRNQV VK in isoform K. Add BLAST | 206 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82640 mRNA Translation: CAA57962.1 X82641 mRNA Translation: CAA57963.1 AE014298 Genomic DNA Translation: AAF46570.1 AE014298 Genomic DNA Translation: AAF46571.3 AE014298 Genomic DNA Translation: AAS65302.1 AE014298 Genomic DNA Translation: AAS65303.1 AE014298 Genomic DNA Translation: AAS65304.1 AE014298 Genomic DNA Translation: AAS65305.1 AE014298 Genomic DNA Translation: AAS65306.1 AE014298 Genomic DNA Translation: AGB95245.1 BT011036 mRNA Translation: AAR30196.1 |
PIRi | S60709 |
RefSeqi | NP_001259402.1, NM_001272473.2 [P53624-2] NP_511105.2, NM_078550.4 [P53624-2] NP_727407.1, NM_167223.3 [P53624-1] NP_996395.1, NM_206672.3 [P53624-1] NP_996396.1, NM_206673.3 [P53624-1] NP_996397.1, NM_206674.3 [P53624-1] NP_996398.1, NM_206675.3 [P53624-1] NP_996399.1, NM_206676.3 [P53624-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0299632; FBpp0288907; FBgn0259170 [P53624-1] FBtr0300511; FBpp0289738; FBgn0259170 [P53624-1] FBtr0300512; FBpp0289739; FBgn0259170 [P53624-2] FBtr0300513; FBpp0289740; FBgn0259170 [P53624-1] FBtr0300514; FBpp0289741; FBgn0259170 [P53624-1] FBtr0300515; FBpp0289742; FBgn0259170 [P53624-1] FBtr0300516; FBpp0289743; FBgn0259170 [P53624-1] FBtr0331759; FBpp0304147; FBgn0259170 [P53624-2] |
GeneIDi | 31957 |
KEGGi | dme:Dmel_CG42275 |
Keywords - Coding sequence diversityi
Alternative promoter usageSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82640 mRNA Translation: CAA57962.1 X82641 mRNA Translation: CAA57963.1 AE014298 Genomic DNA Translation: AAF46570.1 AE014298 Genomic DNA Translation: AAF46571.3 AE014298 Genomic DNA Translation: AAS65302.1 AE014298 Genomic DNA Translation: AAS65303.1 AE014298 Genomic DNA Translation: AAS65304.1 AE014298 Genomic DNA Translation: AAS65305.1 AE014298 Genomic DNA Translation: AAS65306.1 AE014298 Genomic DNA Translation: AGB95245.1 BT011036 mRNA Translation: AAR30196.1 |
PIRi | S60709 |
RefSeqi | NP_001259402.1, NM_001272473.2 [P53624-2] NP_511105.2, NM_078550.4 [P53624-2] NP_727407.1, NM_167223.3 [P53624-1] NP_996395.1, NM_206672.3 [P53624-1] NP_996396.1, NM_206673.3 [P53624-1] NP_996397.1, NM_206674.3 [P53624-1] NP_996398.1, NM_206675.3 [P53624-1] NP_996399.1, NM_206676.3 [P53624-1] |
3D structure databases
AlphaFoldDBi | P53624 |
SMRi | P53624 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 58390, 3 interactors |
IntActi | P53624, 2 interactors |
STRINGi | 7227.FBpp0288908 |
Chemistry databases
BindingDBi | P53624 |
ChEMBLi | CHEMBL1697673 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
PTM databases
GlyGeni | P53624, 1 site |
Proteomic databases
PRIDEi | P53624 |
Protocols and materials databases
DNASUi | 31957 |
Genome annotation databases
EnsemblMetazoai | FBtr0299632; FBpp0288907; FBgn0259170 [P53624-1] FBtr0300511; FBpp0289738; FBgn0259170 [P53624-1] FBtr0300512; FBpp0289739; FBgn0259170 [P53624-2] FBtr0300513; FBpp0289740; FBgn0259170 [P53624-1] FBtr0300514; FBpp0289741; FBgn0259170 [P53624-1] FBtr0300515; FBpp0289742; FBgn0259170 [P53624-1] FBtr0300516; FBpp0289743; FBgn0259170 [P53624-1] FBtr0331759; FBpp0304147; FBgn0259170 [P53624-2] |
GeneIDi | 31957 |
KEGGi | dme:Dmel_CG42275 |
Organism-specific databases
CTDi | 31957 |
FlyBasei | FBgn0259170, alpha-Man-Ia |
VEuPathDBi | VectorBase:FBgn0259170 |
Phylogenomic databases
eggNOGi | KOG2204, Eukaryota |
GeneTreei | ENSGT00940000167910 |
HOGENOMi | CLU_003818_3_2_1 |
InParanoidi | P53624 |
OMAi | LIKMYLY |
PhylomeDBi | P53624 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-DME-964827, Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2 |
Miscellaneous databases
BioGRID-ORCSi | 31957, 1 hit in 3 CRISPR screens |
ChiTaRSi | alpha-Man-Ia, fly |
GenomeRNAii | 31957 |
PROi | PR:P53624 |
Gene expression databases
Bgeei | FBgn0259170, Expressed in embryo and 36 other tissues |
ExpressionAtlasi | P53624, baseline and differential |
Genevisiblei | P53624, DM |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MA1A1_DROME | |
Accessioni | P53624Primary (citable) accession number: P53624 Secondary accession number(s): A4V480 Q9W2W7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | March 15, 2004 | |
Last modified: | May 25, 2022 | |
This is version 177 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families