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Protein

Mannosyl-oligosaccharide alpha-1,2-mannosidase IA

Gene

alpha-Man-Ia

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.By similarity

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+By similarity, Mg2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei529Proton donorBy similarity1
Metal bindingi640CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • determination of adult lifespan Source: FlyBase
  • encapsulation of foreign target Source: FlyBase
  • N-glycan processing Source: GO_Central
  • protein glycosylation Source: UniProtKB-UniPathway
  • response to anesthetic Source: FlyBase

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-964827 Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide alpha-1,2-mannosidase IAImported (EC:3.2.1.113By similarity)
Alternative name(s):
Man(9)-alpha-mannosidase
Mannosidase-1
Gene namesi
Name:alpha-Man-IaImported
Synonyms:alpha-man-1Imported, mas-1Imported
ORF Names:CG32684Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0259170 alpha-Man-Ia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicSequence analysisAdd BLAST18
Transmembranei19 – 39Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini40 – 667LumenalSequence analysisAdd BLAST628

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1697673

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002103161 – 667Mannosyl-oligosaccharide alpha-1,2-mannosidase IAAdd BLAST667

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi278N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi483 ↔ 515By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP53624

Expressioni

Tissue specificityi

Complex spatial distribution during embryogenesis, including expression in lobula plate giant neurons. Also expressed in adult wing and eyes.1 Publication

Developmental stagei

Expressed both maternally and zygotically during embryonic stages.1 Publication

Gene expression databases

BgeeiFBgn0259170 Expressed in 28 organ(s), highest expression level in embryo
ExpressionAtlasiP53624 baseline and differential
GenevisibleiP53624 DM

Interactioni

Protein-protein interaction databases

BioGridi58390, 2 interactors
IntActiP53624, 2 interactors

Chemistry databases

BindingDBiP53624

Structurei

3D structure databases

ProteinModelPortaliP53624
SMRiP53624
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.UniRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016529
InParanoidiP53624
KOiK01230
OMAiQYGWGHN
OrthoDBiEOG091G04C8
PhylomeDBiP53624

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform HImported (identifier: P53624-1) [UniParc]FASTAAdd to basket
Also known as: A1 Publication, JImported, LImported, MImported, NImported, OImported

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MYRISPIGRK SNFHSREKCL IGLVLVTLCF LCFGGIFLLP DNFGSDRVLR
60 70 80 90 100
VYKHFRKAGP EIFIPAPPLA AHAPHRSEDP HFIGDRQRLE QKIRAELGDM
110 120 130 140 150
LDEPPAAGGG EPGQFQVLAQ QAQAPAPVAA LADQPLDQDE GHAAIPVLAA
160 170 180 190 200
PVQGDNAASQ ASSHPQSSAQ QHNQQQPQLP LGGGGNDQAP DTLDATLEER
210 220 230 240 250
RQKVKEMMEH AWHNYKLYAW GKNELRPLSQ RPHSASIFGS YDLGATIVDG
260 270 280 290 300
LDTLYIMGLE KEYREGRDWI ERKFSLDNIS AELSVFETNI RFVGGMLTLY
310 320 330 340 350
AFTGDPLYKE KAQHVADKLL PAFQTPTGIP YALVNTKTGV AKNYGWASGG
360 370 380 390 400
SSILSEFGTL HLEFAYLSDI TGNPLYRERV QTIRQVLKEI EKPKGLYPNF
410 420 430 440 450
LNPKTGKWGQ LHMSLGALGD SYYEYLLKAW LQSGQTDEEA REMFDEAMLA
460 470 480 490 500
ILDKMVRTSP GGLTYVSDLK FDRLEHKMDH LACFSGGLFA LGAATRQNDY
510 520 530 540 550
TDKYMEVGKG ITNTCHESYI RAPTQLGPEA FRFSEAVEAR ALRSQEKYYI
560 570 580 590 600
LRPETFESYF VLWRLTHDQK YRDWGWEAVL ALEKHCRTAH GYCGLRNVYQ
610 620 630 640 650
QEPQKDDVQQ SFFLAETLKY LYLLFSDDSV LPLDEWVFNT EAHPLPIKGA
660
NAYYRQAPVT LPVSNAS
Length:667
Mass (Da):74,966
Last modified:March 15, 2004 - v2
Checksum:iD707F491E4AE47F2
GO
Isoform KImported (identifier: P53624-2) [UniParc] [UniParc]FASTAAdd to basket
Also known as: B1 Publication, PImported

The sequence of this isoform differs from the canonical sequence as follows:
     1-206: MYRISPIGRK...LEERRQKVKE → MCPKTSKTTP...DYEKRNQVVK

Show »
Length:643
Mass (Da):72,525
Checksum:i8725B99D31DE7AC3
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8IRL4Q8IRL4_DROME
alpha-1,2-Mannosidase
alpha-Man-Ia a-Man-Ia, alpha-Man-I, alpha-Man-l, CG17809, CG18799 alpha-Man-Ia a-Man-Ia, alpha-Man-I, alpha-Man-l, CG17809, CG18799, CG32682, CG32684, DM-GI, dManI, dMas-1, Dmel\CG42275, MA12, Man'ase, MAN-1, ManI, mas-1, mas1, CG42275, Dmel_CG42275
684Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21I → T in CAA57962 (PubMed:7729592).Curated1
Sequence conflicti568D → E in CAA57962 (PubMed:7729592).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0579041 – 206MYRIS…QKVKE → MCPKTSKTTPLLLIGGICFV IVLVGITGITLINNINLSNI IRLNEKVASDSVSNNENQIK ELNYVNNHPRNVYLKLNASS RDDEDDEQMQKEQEQLELPK ISAVIGGSKPKVEDNQVKES SEVISPSTSTFSMRSSAGEL TSTSAPLSSIVSVTAPPMPF GGVKYNQSSGLIDYEKRNQV VK in isoform K. Add BLAST206

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82640 mRNA Translation: CAA57962.1
X82641 mRNA Translation: CAA57963.1
AE014298 Genomic DNA Translation: AAF46570.1
AE014298 Genomic DNA Translation: AAF46571.3
AE014298 Genomic DNA Translation: AAS65302.1
AE014298 Genomic DNA Translation: AAS65303.1
AE014298 Genomic DNA Translation: AAS65304.1
AE014298 Genomic DNA Translation: AAS65305.1
AE014298 Genomic DNA Translation: AAS65306.1
AE014298 Genomic DNA Translation: AGB95245.1
BT011036 mRNA Translation: AAR30196.1
PIRiS60709
RefSeqiNP_001259402.1, NM_001272473.2 [P53624-2]
NP_511105.2, NM_078550.4 [P53624-2]
NP_727407.1, NM_167223.3 [P53624-1]
NP_996395.1, NM_206672.3 [P53624-1]
NP_996396.1, NM_206673.3 [P53624-1]
NP_996397.1, NM_206674.3 [P53624-1]
NP_996398.1, NM_206675.3 [P53624-1]
NP_996399.1, NM_206676.3 [P53624-1]
UniGeneiDm.7316

Genome annotation databases

EnsemblMetazoaiFBtr0299632; FBpp0288907; FBgn0259170 [P53624-1]
FBtr0300511; FBpp0289738; FBgn0259170 [P53624-1]
FBtr0300512; FBpp0289739; FBgn0259170 [P53624-2]
FBtr0300513; FBpp0289740; FBgn0259170 [P53624-1]
FBtr0300514; FBpp0289741; FBgn0259170 [P53624-1]
FBtr0300515; FBpp0289742; FBgn0259170 [P53624-1]
FBtr0300516; FBpp0289743; FBgn0259170 [P53624-1]
FBtr0331759; FBpp0304147; FBgn0259170 [P53624-2]
GeneIDi31957
KEGGidme:Dmel_CG42275

Keywords - Coding sequence diversityi

Alternative promoter usage

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82640 mRNA Translation: CAA57962.1
X82641 mRNA Translation: CAA57963.1
AE014298 Genomic DNA Translation: AAF46570.1
AE014298 Genomic DNA Translation: AAF46571.3
AE014298 Genomic DNA Translation: AAS65302.1
AE014298 Genomic DNA Translation: AAS65303.1
AE014298 Genomic DNA Translation: AAS65304.1
AE014298 Genomic DNA Translation: AAS65305.1
AE014298 Genomic DNA Translation: AAS65306.1
AE014298 Genomic DNA Translation: AGB95245.1
BT011036 mRNA Translation: AAR30196.1
PIRiS60709
RefSeqiNP_001259402.1, NM_001272473.2 [P53624-2]
NP_511105.2, NM_078550.4 [P53624-2]
NP_727407.1, NM_167223.3 [P53624-1]
NP_996395.1, NM_206672.3 [P53624-1]
NP_996396.1, NM_206673.3 [P53624-1]
NP_996397.1, NM_206674.3 [P53624-1]
NP_996398.1, NM_206675.3 [P53624-1]
NP_996399.1, NM_206676.3 [P53624-1]
UniGeneiDm.7316

3D structure databases

ProteinModelPortaliP53624
SMRiP53624
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58390, 2 interactors
IntActiP53624, 2 interactors

Chemistry databases

BindingDBiP53624
ChEMBLiCHEMBL1697673

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Proteomic databases

PRIDEiP53624

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0299632; FBpp0288907; FBgn0259170 [P53624-1]
FBtr0300511; FBpp0289738; FBgn0259170 [P53624-1]
FBtr0300512; FBpp0289739; FBgn0259170 [P53624-2]
FBtr0300513; FBpp0289740; FBgn0259170 [P53624-1]
FBtr0300514; FBpp0289741; FBgn0259170 [P53624-1]
FBtr0300515; FBpp0289742; FBgn0259170 [P53624-1]
FBtr0300516; FBpp0289743; FBgn0259170 [P53624-1]
FBtr0331759; FBpp0304147; FBgn0259170 [P53624-2]
GeneIDi31957
KEGGidme:Dmel_CG42275

Organism-specific databases

CTDi31957
FlyBaseiFBgn0259170 alpha-Man-Ia

Phylogenomic databases

GeneTreeiENSGT00390000016529
InParanoidiP53624
KOiK01230
OMAiQYGWGHN
OrthoDBiEOG091G04C8
PhylomeDBiP53624

Enzyme and pathway databases

UniPathwayi
UPA00378

ReactomeiR-DME-964827 Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2

Miscellaneous databases

ChiTaRSialpha-Man-Ia fly
GenomeRNAii31957
PROiPR:P53624

Gene expression databases

BgeeiFBgn0259170 Expressed in 28 organ(s), highest expression level in embryo
ExpressionAtlasiP53624 baseline and differential
GenevisibleiP53624 DM

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMA1A1_DROME
AccessioniPrimary (citable) accession number: P53624
Secondary accession number(s): A4V480
, M9PH54, P53625, Q9W2W6, Q9W2W7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 15, 2004
Last modified: September 12, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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Main funding by: National Institutes of Health

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