UniProtKB - P53622 (COPA_YEAST)
Protein
Coatomer subunit alpha
Gene
COP1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.1 Publication
Miscellaneous
Present with 15200 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- structural molecule activity Source: InterPro
- ubiquitin binding Source: SGD
GO - Biological processi
- endoplasmic reticulum to Golgi vesicle-mediated transport Source: SGD
- intracellular mRNA localization Source: SGD
- intracellular protein transport Source: GO_Central
- intra-Golgi vesicle-mediated transport Source: GO_Central
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: SGD
Keywordsi
Biological process | ER-Golgi transport, Protein transport, Transport |
Enzyme and pathway databases
BioCyci | YEAST:G3O-29542-MONOMER |
Reactomei | R-SCE-6807878, COPI-mediated anterograde transport R-SCE-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Protein family/group databases
TCDBi | 3.A.31.1.1, the endosomal sorting complexes required for transport iii (escrt-iii) family |
Names & Taxonomyi
Protein namesi | Recommended name: Coatomer subunit alphaAlternative name(s): Alpha-coat protein Short name: Alpha-COP Retrieval from endoplasmic reticulum protein 1 Secretory protein 22 Suppressor of osmo-sensitivity 1 |
Gene namesi | Name:COP1 Synonyms:RET1, SEC33, SOO1 Ordered Locus Names:YDL145C ORF Names:D1578 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002304, COP1 |
VEuPathDBi | FungiDB:YDL145C |
Subcellular locationi
Golgi apparatus
Other locations
Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
Golgi apparatus
- COPI vesicle coat Source: SGD
- Golgi membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050910 | 1 – 1201 | Coatomer subunit alphaAdd BLAST | 1201 |
Proteomic databases
MaxQBi | P53622 |
PaxDbi | P53622 |
PRIDEi | P53622 |
PTM databases
CarbonylDBi | P53622 |
iPTMneti | P53622 |
Interactioni
Subunit structurei
Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.
Interacts with the ESCRT-0 subunit VPS27.
Interacts with KEI1 (via C-terminal region).
2 PublicationsBinary interactionsi
Hide detailsP53622
With | #Exp. | IntAct |
---|---|---|
SEC27 [P41811] | 17 | EBI-4860,EBI-4898 |
SEC28 [P40509] | 11 | EBI-4860,EBI-4884 |
WBP1 [P33767] | 3 | EBI-4860,EBI-12658 |
GO - Molecular functioni
- ubiquitin binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 31916, 598 interactors |
ComplexPortali | CPX-1652, COPI vesicle coat complex |
DIPi | DIP-2582N |
IntActi | P53622, 73 interactors |
MINTi | P53622 |
STRINGi | 4932.YDL145C |
Miscellaneous databases
RNActi | P53622, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P53622 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P53622 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 9 – 39 | WD 1Add BLAST | 31 | |
Repeati | 51 – 81 | WD 2Add BLAST | 31 | |
Repeati | 93 – 123 | WD 3Add BLAST | 31 | |
Repeati | 135 – 165 | WD 4Add BLAST | 31 | |
Repeati | 207 – 237 | WD 5Add BLAST | 31 | |
Repeati | 251 – 281 | WD 6Add BLAST | 31 |
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0292, Eukaryota |
GeneTreei | ENSGT00940000155451 |
HOGENOMi | CLU_007565_1_0_1 |
InParanoidi | P53622 |
OMAi | GNQVFCL |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR016391, Coatomer_asu IPR010714, Coatomer_asu_C IPR006692, Coatomer_WD-assoc_reg IPR020472, G-protein_beta_WD-40_rep IPR011044, Quino_amine_DH_bsu IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF04053, Coatomer_WDAD, 1 hit PF06957, COPI_C, 1 hit PF00400, WD40, 5 hits |
PIRSFi | PIRSF003354, Coatomer_alpha_subunit, 1 hit |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50969, SSF50969, 1 hit SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
P53622-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKMLTKFESK STRAKGIAFH PSRPWVLVAL FSSTIQLWDY RMGTLLHRFE
60 70 80 90 100
DHEGPVRGLD FHPTQPIFVS AGDDYTIKVW SLDTNKCLYT LTGHLDYVRT
110 120 130 140 150
VFFHRELPWI ISASDDQTIR IWNWQNRKEI ACLTGHNHFV MCAQFHPTDD
160 170 180 190 200
LIVSASLDET IRIWDISGLR KRHSAPGTSS FEEQMSAQQN LLDGSLGDCV
210 220 230 240 250
VKFILEGHTR GVNWASFHPT LPLIVSGSDD RQVKLWRMSA TKAWEVDTCR
260 270 280 290 300
GHTNNVDSVI FHPHQNLIIS VGEDKTLRVW DLDKRTPVKQ FKRENDRFWL
310 320 330 340 350
IAAHPHINLF GAAHDSGIMV FKLDRERPCS FIHQNQLFFV NAEKQIQSFN
360 370 380 390 400
FQKRVASLPY ASLKGIGQPW DAFRSISYNP SQHSVLVNEA NGKFALVILP
410 420 430 440 450
KQPVGAVEPT SVTQDTGNFA TFVGRNRFVV YNKNTESVEV RSLENKVTRN
460 470 480 490 500
IKVEETVRTI VAAGPGSVLV IHPREVILYD VQQGKKVSQL AVKNVKYVSW
510 520 530 540 550
SLDGQYVALM SKHTITLATK KLELINSMHE TIRIKSAAWD ETGVLIYSTL
560 570 580 590 600
NHIRYSLLNG DRGIIKTLEK TLYITKVQGK LVYCLNREGE IEILTIDPTE
610 620 630 640 650
YRFKKALVNK NFPEVLRLIK DSNLVGQNII SYLQKSGYPE IALQFVQDPH
660 670 680 690 700
IRFDLALEYG NLDVALDEAK KLNDSSTWER LIQEALAQGN ASLAEMIYQT
710 720 730 740 750
QHSFDKLSFL YLVTGDVNKL SKMQNIAQTR EDFGSMLLNT FYNNSTKERS
760 770 780 790 800
SIFAEGGSLP LAYAVAKANG DEAAASAFLE QAEVDEQDVT LPDQMDASNF
810 820 830 840 850
VQRPVISKPL EKWPLKEAEL SYFEKAVLGQ IDDLTIDDET PAVNTTQEQE
860 870 880 890 900
EPLGEENFND EDIGEDEGAW DLGDEDLDVG EELPEEVEQG EITSPAQEVE
910 920 930 940 950
TAIWIKNSKL PAVLVAAGAF DAAVQALSKQ VGVVKLEPLK KYFTNIYEGC
960 970 980 990 1000
RTYIPSTPCE LPAQLGYVRA YDDTVSEDQI LPYVPGLDVV NEKMNEGYKN
1010 1020 1030 1040 1050
FKLNKPDIAI ECFREAIYRI TLLMVDDAED EKLAHKILET AREYILGLSI
1060 1070 1080 1090 1100
ELERRSLKEG NTVRMLELAA YFTKAKLSPI HRTNALQVAM SQHFKHKNFL
1110 1120 1130 1140 1150
QASYFAGEFL KIISSGPRAE QARKIKNKAD SMASDAIPID FDPYAKFDIC
1160 1170 1180 1190 1200
AATYKPIYED TPSVSDPLTG SKYVITEKDK IDRIAMISKI GAPASGLRIR
V
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 753 | F → L in CAA86588 (PubMed:8001155).Curated | 1 | |
Sequence conflicti | 753 | F → L in CAA58712 (PubMed:7724544).Curated | 1 | |
Sequence conflicti | 905 | I → T in CAA86588 (PubMed:8001155).Curated | 1 | |
Sequence conflicti | 905 | I → T in CAA58712 (PubMed:7724544).Curated | 1 | |
Sequence conflicti | 1006 | P → L in CAA86588 (PubMed:8001155).Curated | 1 | |
Sequence conflicti | 1006 | P → L in CAA58712 (PubMed:7724544).Curated | 1 | |
Sequence conflicti | 1027 – 1028 | DA → NT in CAA86588 (PubMed:8001155).Curated | 2 | |
Sequence conflicti | 1027 – 1028 | DA → NT in CAA58712 (PubMed:7724544).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z46617 Genomic DNA Translation: CAA86588.1 X83754 Genomic DNA Translation: CAA58712.1 Z74193 Genomic DNA Translation: CAA98719.1 Z74192 Genomic DNA Translation: CAA98718.1 X97751 Genomic DNA Translation: CAA66346.1 BK006938 Genomic DNA Translation: DAA11713.1 |
PIRi | S67692, ERBYA |
RefSeqi | NP_010136.1, NM_001180205.1 |
Genome annotation databases
EnsemblFungii | YDL145C_mRNA; YDL145C; YDL145C |
GeneIDi | 851410 |
KEGGi | sce:YDL145C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z46617 Genomic DNA Translation: CAA86588.1 X83754 Genomic DNA Translation: CAA58712.1 Z74193 Genomic DNA Translation: CAA98719.1 Z74192 Genomic DNA Translation: CAA98718.1 X97751 Genomic DNA Translation: CAA66346.1 BK006938 Genomic DNA Translation: DAA11713.1 |
PIRi | S67692, ERBYA |
RefSeqi | NP_010136.1, NM_001180205.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3MKQ | X-ray | 2.50 | B/D/F | 642-818 | [»] | |
3MV2 | X-ray | 2.90 | A/C/E | 900-1201 | [»] | |
3MV3 | X-ray | 3.25 | A/C/E | 900-1201 | [»] | |
6U3W | X-ray | 2.39 | A | 899-1201 | [»] | |
SMRi | P53622 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31916, 598 interactors |
ComplexPortali | CPX-1652, COPI vesicle coat complex |
DIPi | DIP-2582N |
IntActi | P53622, 73 interactors |
MINTi | P53622 |
STRINGi | 4932.YDL145C |
Protein family/group databases
TCDBi | 3.A.31.1.1, the endosomal sorting complexes required for transport iii (escrt-iii) family |
PTM databases
CarbonylDBi | P53622 |
iPTMneti | P53622 |
Proteomic databases
MaxQBi | P53622 |
PaxDbi | P53622 |
PRIDEi | P53622 |
Genome annotation databases
EnsemblFungii | YDL145C_mRNA; YDL145C; YDL145C |
GeneIDi | 851410 |
KEGGi | sce:YDL145C |
Organism-specific databases
SGDi | S000002304, COP1 |
VEuPathDBi | FungiDB:YDL145C |
Phylogenomic databases
eggNOGi | KOG0292, Eukaryota |
GeneTreei | ENSGT00940000155451 |
HOGENOMi | CLU_007565_1_0_1 |
InParanoidi | P53622 |
OMAi | GNQVFCL |
Enzyme and pathway databases
BioCyci | YEAST:G3O-29542-MONOMER |
Reactomei | R-SCE-6807878, COPI-mediated anterograde transport R-SCE-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Miscellaneous databases
EvolutionaryTracei | P53622 |
PROi | PR:P53622 |
RNActi | P53622, protein |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR016391, Coatomer_asu IPR010714, Coatomer_asu_C IPR006692, Coatomer_WD-assoc_reg IPR020472, G-protein_beta_WD-40_rep IPR011044, Quino_amine_DH_bsu IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF04053, Coatomer_WDAD, 1 hit PF06957, COPI_C, 1 hit PF00400, WD40, 5 hits |
PIRSFi | PIRSF003354, Coatomer_alpha_subunit, 1 hit |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50969, SSF50969, 1 hit SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | COPA_YEAST | |
Accessioni | P53622Primary (citable) accession number: P53622 Secondary accession number(s): D6VRK3, Q07595 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 194 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references