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Protein

Coatomer subunit alpha

Gene

COPA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).By similarity
Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHormone
Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
SignaLinkiP53621

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit alpha
Alternative name(s):
Alpha-coat protein
Short name:
Alpha-COP
HEP-COP
Short name:
HEPCOP
Cleaved into the following 2 chains:
Alternative name(s):
Xenopsin-related peptide
Gene namesi
Name:COPA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000122218.14
HGNCiHGNC:2230 COPA
MIMi601924 gene
neXtProtiNX_P53621

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Involvement in diseasei

Autoimmune interstitial lung, joint, and kidney disease (AILJK)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autoimmune disease characterized by inflammatory arthritis, interstitial lung disease, and immune complex-mediated renal disease.
See also OMIM:616414
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_073844230K → N in AILJK; causes a defect in retrograde transport from the Golgi to the endoplasmic reticulum. 1 PublicationCorresponds to variant dbSNP:rs864309710EnsemblClinVar.1
Natural variantiVAR_073845233R → H in AILJK. 1 PublicationCorresponds to variant dbSNP:rs794727993EnsemblClinVar.1
Natural variantiVAR_073846241E → K in AILJK; causes a defect in retrograde transport from the Golgi to the endoplasmic reticulum. 1 PublicationCorresponds to variant dbSNP:rs794727995EnsemblClinVar.1
Natural variantiVAR_073847243D → G in AILJK. 1 PublicationCorresponds to variant dbSNP:rs794727994EnsemblClinVar.1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi1314
MalaCardsiCOPA
MIMi616414 phenotype
OpenTargetsiENSG00000122218
PharmGKBiPA26746

Polymorphism and mutation databases

BioMutaiCOPA
DMDMi205371746

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002233071 – 1224Coatomer subunit alphaAdd BLAST1224
PeptideiPRO_00000414001 – 35ProxeninAdd BLAST35
PeptideiPRO_00000414011 – 25XeninAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei173PhosphoserineCombined sources1
Modified residuei185PhosphothreonineCombined sources1
Modified residuei402PhosphoserineCombined sources1
Modified residuei591PhosphothreonineCombined sources1
Modified residuei895PhosphoserineCombined sources1
Modified residuei965Omega-N-methylarginineCombined sources1
Modified residuei1193PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiP53621
MaxQBiP53621
PaxDbiP53621
PeptideAtlasiP53621
PRIDEiP53621
ProteomicsDBi56593
56594 [P53621-2]

PTM databases

iPTMnetiP53621
PhosphoSitePlusiP53621
SwissPalmiP53621

Expressioni

Tissue specificityi

Uniformly expressed in a wide range of adult and fetal tissues. Xenin is found in gastric, duodenal and jejunal mucosa. Circulates in the blood. Seems to be confined to specific endocrine cells.

Developmental stagei

Xenin is released into the circulation after a meal.

Gene expression databases

BgeeiENSG00000122218 Expressed in 240 organ(s), highest expression level in corpus callosum
CleanExiHS_COPA
GenevisibleiP53621 HS

Organism-specific databases

HPAiHPA028024

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Probably interacts with PEX11A. Interacts with SCYL1 (By similarity). Interacts with JAGN1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107709, 104 interactors
IntActiP53621, 70 interactors
MINTiP53621
STRINGi9606.ENSP00000357048

Structurei

3D structure databases

ProteinModelPortaliP53621
SMRiP53621
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati3 – 38WD 1Add BLAST36
Repeati42 – 80WD 2Add BLAST39
Repeati84 – 122WD 3Add BLAST39
Repeati126 – 164WD 4Add BLAST39
Repeati195 – 234WD 5Add BLAST40
Repeati241 – 278WD 6Add BLAST38
Repeati282 – 319WD 7Add BLAST38

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0292 Eukaryota
ENOG410XPZS LUCA
GeneTreeiENSGT00920000149070
HOGENOMiHOG000195913
HOVERGENiHBG005379
InParanoidiP53621
KOiK05236
OMAiGHDNGVM
OrthoDBiEOG091G00SH
PhylomeDBiP53621
TreeFamiTF105693

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR024977 Apc4_WD40_dom
IPR016391 Coatomer_asu
IPR010714 Coatomer_asu_C
IPR006692 Coatomer_WD-assoc_reg
IPR020472 G-protein_beta_WD-40_rep
IPR011048 Haem_d1_sf
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF12894 ANAPC4_WD40, 1 hit
PF04053 Coatomer_WDAD, 1 hit
PF06957 COPI_C, 1 hit
PF00400 WD40, 3 hits
PIRSFiPIRSF003354 Coatomer_alpha_subunit, 1 hit
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 2 hits
SSF51004 SSF51004, 3 hits
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: P53621-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLTKFETKSA RVKGLSFHPK RPWILTSLHN GVIQLWDYRM CTLIDKFDEH
60 70 80 90 100
DGPVRGIDFH KQQPLFVSGG DDYKIKVWNY KLRRCLFTLL GHLDYIRTTF
110 120 130 140 150
FHHEYPWILS ASDDQTIRVW NWQSRTCVCV LTGHNHYVMC AQFHPTEDLV
160 170 180 190 200
VSASLDQTVR VWDISGLRKK NLSPGAVESD VRGITGVDLF GTTDAVVKHV
210 220 230 240 250
LEGHDRGVNW AAFHPTMPLI VSGADDRQVK IWRMNESKAW EVDTCRGHYN
260 270 280 290 300
NVSCAVFHPR QELILSNSED KSIRVWDMSK RTGVQTFRRD HDRFWVLAAH
310 320 330 340 350
PNLNLFAAGH DGGMIVFKLE RERPAYAVHG NMLHYVKDRF LRQLDFNSSK
360 370 380 390 400
DVAVMQLRSG SKFPVFNMSY NPAENAVLLC TRASNLENST YDLYTIPKDA
410 420 430 440 450
DSQNPDAPEG KRSSGLTAVW VARNRFAVLD RMHSLLIKNL KNEITKKVQV
460 470 480 490 500
PNCDEIFYAG TGNLLLRDAD SITLFDVQQK RTLASVKISK VKYVIWSADM
510 520 530 540 550
SHVALLAKHA IVICNRKLDA LCNIHENIRV KSGAWDESGV FIYTTSNHIK
560 570 580 590 600
YAVTTGDHGI IRTLDLPIYV TRVKGNNVYC LDRECRPRVL TIDPTEFKFK
610 620 630 640 650
LALINRKYDE VLHMVRNAKL VGQSIIAYLQ KKGYPEVALH FVKDEKTRFS
660 670 680 690 700
LALECGNIEI ALEAAKALDD KNCWEKLGEV ALLQGNHQIV EMCYQRTKNF
710 720 730 740 750
DKLSFLYLIT GNLEKLRKMM KIAEIRKDMS GHYQNALYLG DVSERVRILK
760 770 780 790 800
NCGQKSLAYL TAATHGLDEE AESLKETFDP EKETIPDIDP NAKLLQPPAP
810 820 830 840 850
IMPLDTNWPL LTVSKGFFEG TIASKGKGGA LAADIDIDTV GTEGWGEDAE
860 870 880 890 900
LQLDEDGFVE ATEGLGDDAL GKGQEEGGGW DVEEDLELPP ELDISPGAAG
910 920 930 940 950
GAEDGFFVPP TKGTSPTQIW CNNSQLPVDH ILAGSFETAM RLLHDQVGVI
960 970 980 990 1000
QFGPYKQLFL QTYARGRTTY QALPCLPSMY GYPNRNWKDA GLKNGVPAVG
1010 1020 1030 1040 1050
LKLNDLIQRL QLCYQLTTVG KFEEAVEKFR SILLSVPLLV VDNKQEIAEA
1060 1070 1080 1090 1100
QQLITICREY IVGLSVETER KKLPKETLEQ QKRICEMAAY FTHSNLQPVH
1110 1120 1130 1140 1150
MILVLRTALN LFFKLKNFKT AATFARRLLE LGPKPEVAQQ TRKILSACEK
1160 1170 1180 1190 1200
NPTDAYQLNY DMHNPFDICA ASYRPIYRGK PVEKCPLSGA CYSPEFKGQI
1210 1220
CRVTTVTEIG KDVIGLRISP LQFR
Length:1,224
Mass (Da):138,346
Last modified:September 2, 2008 - v2
Checksum:i5A8BC35CE78F155D
GO
Isoform 2 (identifier: P53621-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     509-509: H → HEHSCPLPLT

Show »
Length:1,233
Mass (Da):139,324
Checksum:iF43D084894C9AD98
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti703L → V in AAB70879 (PubMed:8647451).Curated1

RNA editingi

Edited at position 164.1 Publication
Edited at about 31%.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_066525164I → V in RNA edited version. 1
Natural variantiVAR_073844230K → N in AILJK; causes a defect in retrograde transport from the Golgi to the endoplasmic reticulum. 1 PublicationCorresponds to variant dbSNP:rs864309710EnsemblClinVar.1
Natural variantiVAR_073845233R → H in AILJK. 1 PublicationCorresponds to variant dbSNP:rs794727993EnsemblClinVar.1
Natural variantiVAR_073846241E → K in AILJK; causes a defect in retrograde transport from the Golgi to the endoplasmic reticulum. 1 PublicationCorresponds to variant dbSNP:rs794727995EnsemblClinVar.1
Natural variantiVAR_073847243D → G in AILJK. 1 PublicationCorresponds to variant dbSNP:rs794727994EnsemblClinVar.1
Natural variantiVAR_0338031040V → G. Corresponds to variant dbSNP:rs34997807Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_035043509H → HEHSCPLPLT in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24105 mRNA Translation: AAB70879.1
AL513282 Genomic DNA No translation available.
AL445230 Genomic DNA No translation available.
CH471121 Genomic DNA Translation: EAW52723.1
CH471121 Genomic DNA Translation: EAW52725.1
BC038447 mRNA Translation: AAH38447.1
CCDSiCCDS1202.1 [P53621-1]
CCDS41424.1 [P53621-2]
PIRiJC4668 ERHUAH
RefSeqiNP_001091868.1, NM_001098398.1 [P53621-2]
NP_004362.2, NM_004371.3 [P53621-1]
UniGeneiHs.162121
Hs.685025

Genome annotation databases

EnsembliENST00000241704; ENSP00000241704; ENSG00000122218 [P53621-1]
ENST00000368069; ENSP00000357048; ENSG00000122218 [P53621-2]
GeneIDi1314
KEGGihsa:1314
UCSCiuc001fvv.5 human [P53621-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism, RNA editing

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

Xenin entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24105 mRNA Translation: AAB70879.1
AL513282 Genomic DNA No translation available.
AL445230 Genomic DNA No translation available.
CH471121 Genomic DNA Translation: EAW52723.1
CH471121 Genomic DNA Translation: EAW52725.1
BC038447 mRNA Translation: AAH38447.1
CCDSiCCDS1202.1 [P53621-1]
CCDS41424.1 [P53621-2]
PIRiJC4668 ERHUAH
RefSeqiNP_001091868.1, NM_001098398.1 [P53621-2]
NP_004362.2, NM_004371.3 [P53621-1]
UniGeneiHs.162121
Hs.685025

3D structure databases

ProteinModelPortaliP53621
SMRiP53621
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107709, 104 interactors
IntActiP53621, 70 interactors
MINTiP53621
STRINGi9606.ENSP00000357048

PTM databases

iPTMnetiP53621
PhosphoSitePlusiP53621
SwissPalmiP53621

Polymorphism and mutation databases

BioMutaiCOPA
DMDMi205371746

Proteomic databases

EPDiP53621
MaxQBiP53621
PaxDbiP53621
PeptideAtlasiP53621
PRIDEiP53621
ProteomicsDBi56593
56594 [P53621-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000241704; ENSP00000241704; ENSG00000122218 [P53621-1]
ENST00000368069; ENSP00000357048; ENSG00000122218 [P53621-2]
GeneIDi1314
KEGGihsa:1314
UCSCiuc001fvv.5 human [P53621-1]

Organism-specific databases

CTDi1314
DisGeNETi1314
EuPathDBiHostDB:ENSG00000122218.14
GeneCardsiCOPA
HGNCiHGNC:2230 COPA
HPAiHPA028024
MalaCardsiCOPA
MIMi601924 gene
616414 phenotype
neXtProtiNX_P53621
OpenTargetsiENSG00000122218
PharmGKBiPA26746
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0292 Eukaryota
ENOG410XPZS LUCA
GeneTreeiENSGT00920000149070
HOGENOMiHOG000195913
HOVERGENiHBG005379
InParanoidiP53621
KOiK05236
OMAiGHDNGVM
OrthoDBiEOG091G00SH
PhylomeDBiP53621
TreeFamiTF105693

Enzyme and pathway databases

ReactomeiR-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
SignaLinkiP53621

Miscellaneous databases

ChiTaRSiCOPA human
GeneWikiiCOPA_(gene)
GenomeRNAii1314
PROiPR:P53621
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000122218 Expressed in 240 organ(s), highest expression level in corpus callosum
CleanExiHS_COPA
GenevisibleiP53621 HS

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR024977 Apc4_WD40_dom
IPR016391 Coatomer_asu
IPR010714 Coatomer_asu_C
IPR006692 Coatomer_WD-assoc_reg
IPR020472 G-protein_beta_WD-40_rep
IPR011048 Haem_d1_sf
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF12894 ANAPC4_WD40, 1 hit
PF04053 Coatomer_WDAD, 1 hit
PF06957 COPI_C, 1 hit
PF00400 WD40, 3 hits
PIRSFiPIRSF003354 Coatomer_alpha_subunit, 1 hit
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 2 hits
SSF51004 SSF51004, 3 hits
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCOPA_HUMAN
AccessioniPrimary (citable) accession number: P53621
Secondary accession number(s): Q5T201, Q8IXZ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 2, 2008
Last modified: October 10, 2018
This is version 197 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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