UniProtKB - P53612 (PGTB2_MOUSE)
Geranylgeranyl transferase type-2 subunit beta
Rabggtb
Functioni
Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of Rab proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX, such as RAB1A, RAB3A, RAB5A and RAB7A.
1 PublicationCatalytic activityi
- geranylgeranyl diphosphate + L-cysteinyl-[protein] = diphosphate + S-geranylgeranyl-L-cysteinyl-[protein]1 PublicationEC:2.5.1.601 Publication
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 246 | Zinc; catalytic1 Publication | 1 | |
Metal bindingi | 248 | Zinc; catalytic1 Publication | 1 | |
Metal bindingi | 298 | Zinc; via tele nitrogen; catalytic1 Publication | 1 |
GO - Molecular functioni
- isoprenoid binding Source: MGI
- Rab geranylgeranyltransferase activity Source: UniProtKB
- small GTPase binding Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- protein geranylgeranylation Source: UniProtKB
- protein prenylation Source: GO_Central
Keywordsi
Molecular function | Prenyltransferase, Transferase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-6803205, TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain R-MMU-8873719, RAB geranylgeranylation |
Names & Taxonomyi
Protein namesi | Recommended name: Geranylgeranyl transferase type-2 subunit beta (EC:2.5.1.60)Alternative name(s): Geranylgeranyl transferase type II subunit beta Short name: GGTase-II-beta Rab geranyl-geranyltransferase subunit beta Short name: Rab GG transferase beta Short name: Rab GGTase beta Rab geranylgeranyltransferase subunit beta Type II protein geranyl-geranyltransferase subunit beta |
Gene namesi | Name:Rabggtb |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99537, Rabggtb |
VEuPathDBi | HostDB:ENSMUSG00000038975 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119773 | 1 – 339 | Geranylgeranyl transferase type-2 subunit betaAdd BLAST | 339 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 11 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P53612 |
MaxQBi | P53612 |
PaxDbi | P53612 |
PeptideAtlasi | P53612 |
PRIDEi | P53612 |
ProteomicsDBi | 288135 |
PTM databases
iPTMneti | P53612 |
PhosphoSitePlusi | P53612 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000038975, Expressed in cumulus cell and 316 other tissues |
Genevisiblei | P53612, MM |
Interactioni
Subunit structurei
Heterotrimer composed of RABGGTA, RABGGTB and CHM; within this trimer, RABGGTA and RABGGTB form the catalytic component B, while CHM (component A) mediates peptide substrate binding (PubMed:21520375). The Rab GGTase dimer (RGGT) interacts with CHM (component A) prior to Rab protein binding; the association is stabilized by geranylgeranyl pyrophosphate (GGpp) (By similarity). The CHM:RGGT:Rab complex is destabilized by GGpp (By similarity). Interaction of RABGGTB with prenylated PTP4A2 precludes its association with RABGGTA and inhibits enzyme activity (By similarity).
Interacts with CHODL (PubMed:18161010).
Interacts with non-phosphorylated form of RAB8A; phosphorylation of RAB8A at 'Thr-72' disrupts this interaction (By similarity).
By similarity2 PublicationsBinary interactionsi
P53612
With | #Exp. | IntAct |
---|---|---|
Chodl [Q9CXM0] | 2 | EBI-9104297,EBI-13948582 |
GO - Molecular functioni
- small GTPase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 202555, 9 interactors |
ComplexPortali | CPX-2920, Protein geranylgeranyltransferase type II complex |
IntActi | P53612, 1 interactor |
STRINGi | 10090.ENSMUSP00000129481 |
Miscellaneous databases
RNActi | P53612, protein |
Structurei
3D structure databases
SMRi | P53612 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P53612 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 28 – 69 | PFTB 1Add BLAST | 42 | |
Repeati | 76 – 117 | PFTB 2Add BLAST | 42 | |
Repeati | 124 – 165 | PFTB 3Add BLAST | 42 | |
Repeati | 172 – 213 | PFTB 4Add BLAST | 42 | |
Repeati | 220 – 261 | PFTB 5Add BLAST | 42 | |
Repeati | 268 – 310 | PFTB 6Add BLAST | 43 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 198 – 200 | Geranylgeranyl diphosphate bindingBy similarity | 3 | |
Regioni | 240 – 252 | Geranylgeranyl diphosphate bindingBy similarityAdd BLAST | 13 | |
Regioni | 249 – 252 | Geranylgeranyl diphosphate bindingBy similarity | 4 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0366, Eukaryota |
GeneTreei | ENSGT00950000183128 |
InParanoidi | P53612 |
OrthoDBi | 1042804at2759 |
PhylomeDBi | P53612 |
TreeFami | TF105762 |
Family and domain databases
CDDi | cd02894, GGTase-II, 1 hit |
InterProi | View protein in InterPro IPR001330, PFTB_repeat IPR045089, PGGT1B-like IPR026873, Ptb1 IPR008930, Terpenoid_cyclase/PrenylTrfase |
PANTHERi | PTHR11774, PTHR11774, 1 hit |
Pfami | View protein in Pfam PF00432, Prenyltrans, 5 hits |
SUPFAMi | SSF48239, SSF48239, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGSLLFSWKG TQQKDVTIKS DAPDTLLLEK HADYIASYGS KKDDYEYCMS
60 70 80 90 100
EYLRMSGVYW GLTVMDLMGQ LHRMNREEIL VFIKSCQHEC GGISASIGHD
110 120 130 140 150
PHLLYTLSAV QILTLYDSVH VINVDKVVAY VQSLQKEDGS FAGDIWGEID
160 170 180 190 200
TRFSFCAVAT LALLGKLDAI NVEKAIEFVL SCMNFDGGFG CRPGSESHAG
210 220 230 240 250
QIYCCTGFLA ITSQLHQVNS DLLGWWLCER QLPSGGLNGR PEKLPDVCYS
260 270 280 290 300
WWVLASLKII GRLHWIDREK LRSFILACQD EETGGFADRP GDMVDPFHTL
310 320 330
FGIAGLSLLG EEQIKPVSPV FCMPEEVLQR VNVQPELVS
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3TVF4 | Q3TVF4_MOUSE | Geranylgeranyl transferase type-2 s... | Rabggtb | 331 | Annotation score: | ||
A0A0G2JG79 | A0A0G2JG79_MOUSE | Geranylgeranyl transferase type-2 s... | Rabggtb | 205 | Annotation score: | ||
Q6PFA1 | Q6PFA1_MOUSE | Geranylgeranyl transferase type-2 s... | Rabggtb | 132 | Annotation score: | ||
A0A0G2JF29 | A0A0G2JF29_MOUSE | Geranylgeranyl transferase type-2 s... | Rabggtb | 162 | Annotation score: | ||
Q9JIF6 | Q9JIF6_MOUSE | Geranylgeranyl transferase type-2 s... | Rabggtb | 153 | Annotation score: | ||
A0A0G2JDK3 | A0A0G2JDK3_MOUSE | Geranylgeranyl transferase type-2 s... | Rabggtb | 149 | Annotation score: | ||
A0A0G2JGU4 | A0A0G2JGU4_MOUSE | Geranylgeranyl transferase type-2 s... | Rabggtb | 152 | Annotation score: | ||
A0A0G2JFP7 | A0A0G2JFP7_MOUSE | Geranylgeranyl transferase type-2 s... | Rabggtb | 59 | Annotation score: | ||
A0A0G2JGH5 | A0A0G2JGH5_MOUSE | Geranylgeranyl transferase type-2 s... | Rabggtb | 80 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 230 | R → A in AAB01502 (PubMed:7544156).Curated | 1 | |
Sequence conflicti | 236 | G → A in AAB01502 (PubMed:7544156).Curated | 1 | |
Sequence conflicti | 241 | P → R in AAB01502 (PubMed:7544156).Curated | 1 | |
Sequence conflicti | 267 – 268 | DR → VS in AAB01502 (PubMed:7544156).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U12922 mRNA Translation: AAB01502.1 CH466532 Genomic DNA Translation: EDL11902.1 BC132473 mRNA Translation: AAI32474.1 BC138547 mRNA Translation: AAI38548.1 |
CCDSi | CCDS17923.1 |
PIRi | I49116 JC6177 |
RefSeqi | NP_035361.2, NM_011231.2 |
Genome annotation databases
Ensembli | ENSMUST00000167111; ENSMUSP00000129481; ENSMUSG00000038975 |
GeneIDi | 19352 |
KEGGi | mmu:19352 |
UCSCi | uc008rug.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U12922 mRNA Translation: AAB01502.1 CH466532 Genomic DNA Translation: EDL11902.1 BC132473 mRNA Translation: AAI32474.1 BC138547 mRNA Translation: AAI38548.1 |
CCDSi | CCDS17923.1 |
PIRi | I49116 JC6177 |
RefSeqi | NP_035361.2, NM_011231.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3PZ1 | X-ray | 1.95 | B | 10-339 | [»] | |
3PZ2 | X-ray | 2.35 | B | 10-339 | [»] | |
3PZ3 | X-ray | 2.00 | B | 10-339 | [»] | |
SMRi | P53612 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202555, 9 interactors |
ComplexPortali | CPX-2920, Protein geranylgeranyltransferase type II complex |
IntActi | P53612, 1 interactor |
STRINGi | 10090.ENSMUSP00000129481 |
PTM databases
iPTMneti | P53612 |
PhosphoSitePlusi | P53612 |
Proteomic databases
EPDi | P53612 |
MaxQBi | P53612 |
PaxDbi | P53612 |
PeptideAtlasi | P53612 |
PRIDEi | P53612 |
ProteomicsDBi | 288135 |
Protocols and materials databases
Antibodypediai | 19716, 68 antibodies from 23 providers |
DNASUi | 19352 |
Genome annotation databases
Ensembli | ENSMUST00000167111; ENSMUSP00000129481; ENSMUSG00000038975 |
GeneIDi | 19352 |
KEGGi | mmu:19352 |
UCSCi | uc008rug.2, mouse |
Organism-specific databases
CTDi | 5876 |
MGIi | MGI:99537, Rabggtb |
VEuPathDBi | HostDB:ENSMUSG00000038975 |
Phylogenomic databases
eggNOGi | KOG0366, Eukaryota |
GeneTreei | ENSGT00950000183128 |
InParanoidi | P53612 |
OrthoDBi | 1042804at2759 |
PhylomeDBi | P53612 |
TreeFami | TF105762 |
Enzyme and pathway databases
Reactomei | R-MMU-6803205, TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain R-MMU-8873719, RAB geranylgeranylation |
Miscellaneous databases
BioGRID-ORCSi | 19352, 25 hits in 60 CRISPR screens |
ChiTaRSi | Rabggtb, mouse |
EvolutionaryTracei | P53612 |
PROi | PR:P53612 |
RNActi | P53612, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038975, Expressed in cumulus cell and 316 other tissues |
Genevisiblei | P53612, MM |
Family and domain databases
CDDi | cd02894, GGTase-II, 1 hit |
InterProi | View protein in InterPro IPR001330, PFTB_repeat IPR045089, PGGT1B-like IPR026873, Ptb1 IPR008930, Terpenoid_cyclase/PrenylTrfase |
PANTHERi | PTHR11774, PTHR11774, 1 hit |
Pfami | View protein in Pfam PF00432, Prenyltrans, 5 hits |
SUPFAMi | SSF48239, SSF48239, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PGTB2_MOUSE | |
Accessioni | P53612Primary (citable) accession number: P53612 Secondary accession number(s): A2RTE6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 151 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families