UniProtKB - P53610 (PGTB1_RAT)
Protein
Geranylgeranyl transferase type-1 subunit beta
Gene
Pggt1b
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to a cysteine at the fourth position from the C-terminus of proteins with the C-terminal sequence Cys-aliphatic-aliphatic-X. Known substrates include RAC1, RAC2, RAP1A and RAP1B.1 Publication
Catalytic activityi
- geranylgeranyl diphosphate + L-cysteinyl-[protein] = diphosphate + S-geranylgeranyl-L-cysteinyl-[protein]1 PublicationEC:2.5.1.591 Publication
Cofactori
Protein has several cofactor binding sites:Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 269 | Zinc; catalyticCombined sources1 Publication | 1 | |
Metal bindingi | 271 | Zinc; catalyticCombined sources1 Publication | 1 | |
Metal bindingi | 321 | Zinc; via tele nitrogen; catalyticCombined sources1 Publication | 1 |
GO - Molecular functioni
- CAAX-protein geranylgeranyltransferase activity Source: UniProtKB
- protein geranylgeranyltransferase activity Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- negative regulation of nitric-oxide synthase biosynthetic process Source: RGD
- positive regulation of cell cycle Source: RGD
- positive regulation of cell population proliferation Source: RGD
- protein geranylgeranylation Source: UniProtKB
- protein prenylation Source: GO_Central
- response to cytokine Source: RGD
Keywordsi
Molecular function | Prenyltransferase, Transferase |
Ligand | Magnesium, Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Geranylgeranyl transferase type-1 subunit beta (EC:2.5.1.59)Alternative name(s): Geranylgeranyl transferase type I subunit beta Short name: GGTase-I-beta Type I protein geranyl-geranyltransferase subunit beta |
Gene namesi | Name:Pggt1b |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621754, Pggt1b |
Subcellular locationi
Other locations
- CAAX-protein geranylgeranyltransferase complex Source: UniProtKB
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119771 | 1 – 377 | Geranylgeranyl transferase type-1 subunit betaAdd BLAST | 377 |
Proteomic databases
PaxDbi | P53610 |
PRIDEi | P53610 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000003541, Expressed in ileum and 21 other tissues |
Genevisiblei | P53610, RN |
Interactioni
Subunit structurei
Heterodimer of FNTA and PGGT1B. PGGT1B mediates interaction with substrate peptides.
3 PublicationsBinary interactionsi
P53610
With | #Exp. | IntAct |
---|---|---|
Fnta [Q04631] | 7 | EBI-602610,EBI-602447 |
Protein-protein interaction databases
CORUMi | P53610 |
DIPi | DIP-33953N |
IntActi | P53610, 2 interactors |
STRINGi | 10116.ENSRNOP00000004723 |
Chemistry databases
BindingDBi | P53610 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P53610 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P53610 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 144 – 186 | PFTB 1Sequence analysisAdd BLAST | 43 | |
Repeati | 193 – 234 | PFTB 2Sequence analysisAdd BLAST | 42 | |
Repeati | 245 – 284 | PFTB 3Sequence analysisAdd BLAST | 40 | |
Repeati | 291 – 333 | PFTB 4Sequence analysisAdd BLAST | 43 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 219 – 221 | Geranylgeranyl diphosphate bindingCombined sources1 Publication | 3 | |
Regioni | 263 – 266 | Geranylgeranyl diphosphate bindingCombined sources1 Publication | 4 | |
Regioni | 272 – 275 | Geranylgeranyl diphosphate bindingCombined sources1 Publication | 4 |
Sequence similaritiesi
Belongs to the protein prenyltransferase subunit beta family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0367, Eukaryota |
GeneTreei | ENSGT00950000183128 |
HOGENOMi | CLU_028946_2_2_1 |
InParanoidi | P53610 |
OMAi | RKYQWGH |
OrthoDBi | 1042804at2759 |
PhylomeDBi | P53610 |
TreeFami | TF105968 |
Family and domain databases
CDDi | cd02895, GGTase-I, 1 hit |
InterProi | View protein in InterPro IPR041960, GGTase_I_beta IPR001330, PFTB_repeat IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF00432, Prenyltrans, 4 hits |
SUPFAMi | SSF48239, SSF48239, 1 hit |
i Sequence
Sequence statusi: Complete.
P53610-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAATEDDRLA GSGEGERLDF LRDRHVRFFQ RCLQVLPERY SSLETSRLTI
60 70 80 90 100
AFFALSGLDM LDSLDVVNKD DIIEWIYSLQ VLPTEDRSNL DRCGFRGSSY
110 120 130 140 150
LGIPFNPSKN PGTAHPYDSG HIAMTYTGLS CLIILGDDLS RVDKEACLAG
160 170 180 190 200
LRALQLEDGS FCAVPEGSEN DMRFVYCASC ICYMLNNWSG MDMKKAISYI
210 220 230 240 250
RRSMSYDNGL AQGAGLESHG GSTFCGIASL CLMGKLEEVF SEKELNRIKR
260 270 280 290 300
WCIMRQQNGY HGRPNKPVDT CYSFWVGATL KLLKIFQYTN FEKNRNYILS
310 320 330 340 350
TQDRLVGGFA KWPDSHPDAL HAYFGICGLS LMEESGICKV HPALNVSTRT
360 370
SERLRDLHQS WKTKDSKQCS DNVHISS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L24116 mRNA Translation: AAA17756.1 |
PIRi | B53044 |
RefSeqi | NP_112344.1, NM_031082.1 |
Genome annotation databases
Ensembli | ENSRNOT00000004723; ENSRNOP00000004723; ENSRNOG00000003541 |
GeneIDi | 81746 |
KEGGi | rno:81746 |
UCSCi | RGD:621754, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L24116 mRNA Translation: AAA17756.1 |
PIRi | B53044 |
RefSeqi | NP_112344.1, NM_031082.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1N4P | X-ray | 2.65 | B/D/F/H/J/L | 1-377 | [»] | |
1N4Q | X-ray | 2.40 | B/D/F/H/J/L | 1-377 | [»] | |
1N4R | X-ray | 2.80 | B/D/F/H/J/L | 1-377 | [»] | |
1N4S | X-ray | 2.60 | B/D/F/H/J/L | 1-377 | [»] | |
1S64 | X-ray | 2.55 | B/D/F/H/J/L | 1-377 | [»] | |
1TNB | X-ray | 2.85 | B/D/F/H/J/L | 1-377 | [»] | |
1TNO | X-ray | 2.70 | B/D/F/H/J/L | 1-377 | [»] | |
1TNU | X-ray | 2.70 | B/D/F/H/J/L | 1-377 | [»] | |
1TNY | X-ray | 2.70 | B/D/F/H/J/L | 1-377 | [»] | |
1TNZ | X-ray | 2.90 | B/D/F/H/J/L | 1-377 | [»] | |
SMRi | P53610 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
CORUMi | P53610 |
DIPi | DIP-33953N |
IntActi | P53610, 2 interactors |
STRINGi | 10116.ENSRNOP00000004723 |
Chemistry databases
BindingDBi | P53610 |
ChEMBLi | CHEMBL2111479 |
Proteomic databases
PaxDbi | P53610 |
PRIDEi | P53610 |
Genome annotation databases
Ensembli | ENSRNOT00000004723; ENSRNOP00000004723; ENSRNOG00000003541 |
GeneIDi | 81746 |
KEGGi | rno:81746 |
UCSCi | RGD:621754, rat |
Organism-specific databases
CTDi | 5229 |
RGDi | 621754, Pggt1b |
Phylogenomic databases
eggNOGi | KOG0367, Eukaryota |
GeneTreei | ENSGT00950000183128 |
HOGENOMi | CLU_028946_2_2_1 |
InParanoidi | P53610 |
OMAi | RKYQWGH |
OrthoDBi | 1042804at2759 |
PhylomeDBi | P53610 |
TreeFami | TF105968 |
Miscellaneous databases
EvolutionaryTracei | P53610 |
PROi | PR:P53610 |
Gene expression databases
Bgeei | ENSRNOG00000003541, Expressed in ileum and 21 other tissues |
Genevisiblei | P53610, RN |
Family and domain databases
CDDi | cd02895, GGTase-I, 1 hit |
InterProi | View protein in InterPro IPR041960, GGTase_I_beta IPR001330, PFTB_repeat IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF00432, Prenyltrans, 4 hits |
SUPFAMi | SSF48239, SSF48239, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PGTB1_RAT | |
Accessioni | P53610Primary (citable) accession number: P53610 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families