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Protein

Biotin synthase

Gene

bioB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi65Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi69Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi72Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi109Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi141Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi201Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi271Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU30200-MONOMER
UniPathwayi
UPA00078;UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:BSU30200
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001855461 – 335Biotin synthaseAdd BLAST335

Proteomic databases

PaxDbiP53557
PRIDEiP53557

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiP53557, 1 interactor
MINTiP53557
STRINGi224308.Bsubs1_010100016451

Structurei

3D structure databases

ProteinModelPortaliP53557
SMRiP53557
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
HOGENOMiHOG000239958
InParanoidiP53557
KOiK01012
OMAiADRFCMG
PhylomeDBiP53557

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

P53557-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNQWMELADR VLAGAEVTDE EALSILHCPD EDILLLMHGA FHIRKHFYGK
60 70 80 90 100
KVKLNMIMNA KSGLCPENCG YCSQSAISKA PIESYRMVNK ETLLEGAKRA
110 120 130 140 150
HDLNIGTYCI VASGRGPSNR EVDQVVDAVQ EIKETYGLKI CACLGLLKPE
160 170 180 190 200
QAKRLKDAGV DRYNHNLNTS QRNHSNITTS HTYDDRVNTV EIAKESGLSP
210 220 230 240 250
CSGAIIGMKE TKQDVIDIAK SLKALDADSI PVNFLHAIDG TPLEGVNELN
260 270 280 290 300
PLYCLKVLAL FRFINPSKEI RISGGREVNL RTLQPLGLYA ANSIFVGDYL
310 320 330
TTAGQEETED HKMLSDLGFE VESVEEMKAS LSAKS
Length:335
Mass (Da):36,927
Last modified:October 1, 1996 - v1
Checksum:iD4B2E1A53271ED26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51868 Genomic DNA Translation: AAB17461.1
AF008220 Genomic DNA Translation: AAC00265.1
AL009126 Genomic DNA Translation: CAB14998.1
PIRiD69594
RefSeqiNP_390898.1, NC_000964.3
WP_004398967.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14998; CAB14998; BSU30200
GeneIDi938180
KEGGibsu:BSU30200
PATRICifig|224308.179.peg.3276

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51868 Genomic DNA Translation: AAB17461.1
AF008220 Genomic DNA Translation: AAC00265.1
AL009126 Genomic DNA Translation: CAB14998.1
PIRiD69594
RefSeqiNP_390898.1, NC_000964.3
WP_004398967.1, NZ_JNCM01000036.1

3D structure databases

ProteinModelPortaliP53557
SMRiP53557
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP53557, 1 interactor
MINTiP53557
STRINGi224308.Bsubs1_010100016451

Proteomic databases

PaxDbiP53557
PRIDEiP53557

Protocols and materials databases

DNASUi938180
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14998; CAB14998; BSU30200
GeneIDi938180
KEGGibsu:BSU30200
PATRICifig|224308.179.peg.3276

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
HOGENOMiHOG000239958
InParanoidiP53557
KOiK01012
OMAiADRFCMG
PhylomeDBiP53557

Enzyme and pathway databases

UniPathwayi
UPA00078;UER00162

BioCyciBSUB:BSU30200-MONOMER

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBIOB_BACSU
AccessioniPrimary (citable) accession number: P53557
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 10, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
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Main funding by: National Institutes of Health

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