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Protein

Aspartokinase

Gene

lysC

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids threonine, isoleucine and methionine.By similarity

Catalytic activityi

ATP + L-aspartate = ADP + 4-phospho-L-aspartate.

Enzyme regulationi

Lysine-sensitive.By similarity

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (GT94_03850), Aspartokinase (AA904_06710), Aspartokinase (lysC), Aspartokinase (yclM), Aspartokinase (GS458_3084), Aspartokinase (AA904_06830), Aspartokinase (B4109_1081), Aspartokinase (lysC), Aspartokinase (B4114_2755), Aspartokinase (B4114_0875)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA), 4-hydroxy-tetrahydrodipicolinate synthase (dapA), 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB), 4-hydroxy-tetrahydrodipicolinate reductase (dapB), 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-homoserine from L-aspartate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Aspartokinase (GT94_03850), Aspartokinase (AA904_06710), Aspartokinase (lysC), Aspartokinase (yclM), Aspartokinase (GS458_3084), Aspartokinase (AA904_06830), Aspartokinase (B4109_1081), Aspartokinase (lysC), Aspartokinase (B4114_2755), Aspartokinase (B4114_0875)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Homoserine dehydrogenase (GT94_10700), Homoserine dehydrogenase (GS458_2869), Homoserine dehydrogenase (B4114_2542), Homoserine dehydrogenase (hom), Homoserine dehydrogenase (AA904_14675)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homoserine from L-aspartate, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (GT94_03850), Aspartokinase (AA904_06710), Aspartokinase (lysC), Aspartokinase (yclM), Aspartokinase (GS458_3084), Aspartokinase (AA904_06830), Aspartokinase (B4109_1081), Aspartokinase (lysC), Aspartokinase (B4114_2755), Aspartokinase (B4114_0875)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Homoserine dehydrogenase (GT94_10700), Homoserine dehydrogenase (GS458_2869), Homoserine dehydrogenase (B4114_2542), Homoserine dehydrogenase (hom), Homoserine dehydrogenase (AA904_14675)
  4. Homoserine kinase (thrB_1), Homoserine kinase (thrB), Homoserine kinase (thrB), Homoserine kinase (thrB), Homoserine kinase (thrB)
  5. Threonine synthase (thrC_1)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei7Contribution to the catalysisBy similarity1
Binding sitei41ATPBy similarity1
Binding sitei74SubstrateBy similarity1
Sitei74Contribution to the catalysisBy similarity1
Binding sitei153SubstrateBy similarity1
Binding sitei295SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00034; UER00015
UPA00050; UER00461
UPA00051; UER00462

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartokinase (EC:2.7.2.4)
Alternative name(s):
Aspartate kinase
Gene namesi
Name:lysC
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000023711 – 407AspartokinaseAdd BLAST407

Interactioni

Subunit structurei

Tetramer consisting of 2 isoforms Alpha (catalytic and regulation) and of a homodimer of 2 isoforms Beta (regulation).By similarity

Structurei

3D structure databases

ProteinModelPortaliP53553
SMRiP53553
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini264 – 338ACT 1PROSITE-ProRule annotationAdd BLAST75
Domaini340 – 407ACT 2PROSITE-ProRule annotationAdd BLAST68

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 10ATP bindingBy similarity4
Regioni25 – 30Substrate bindingBy similarity6
Regioni47 – 49Substrate bindingBy similarity3
Regioni125 – 126Substrate bindingBy similarity2
Regioni150 – 153Substrate bindingBy similarity4
Regioni173 – 174ATP bindingBy similarity2
Regioni179 – 184ATP bindingBy similarity6
Regioni289 – 291Substrate bindingBy similarity3
Regioni351 – 352Substrate bindingBy similarity2
Regioni365 – 366Substrate bindingBy similarity2
Regioni372 – 373Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the aspartokinase family.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR002912 ACT_dom
IPR001048 Asp/Glu/Uridylate_kinase
IPR005260 Asp_kin_monofn
IPR001341 Asp_kinase
IPR018042 Aspartate_kinase_CS
IPR027795 CASTOR_ACT_dom
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01842 ACT, 1 hit
PF13840 ACT_7, 1 hit
PIRSFiPIRSF000726 Asp_kin, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR00656 asp_kin_monofn, 1 hit
TIGR00657 asp_kinases, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS00324 ASPARTOKINASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Alpha (identifier: P53553-1) [UniParc]FASTAAdd to basket
Also known as: Aspartokinase 2 subunit alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGIIVQKFGG TSVGSIERIQ HVANRVIEEV QKGNDVVVVV SAMGKTTDEL
60 70 80 90 100
VNLAKQISNH PSKREMDMLL STGEQVSIAL LAMSLHEKGY KAVSLTGWQA
110 120 130 140 150
GITTEEMHGN ARIMNIDTTR IRRCLDEGAI VIVAGFQGVT ETGEITTLGR
160 170 180 190 200
GGSDTTAVAL AAALKAEKCD IYTDVTGVFT TDPRYVKTAR KIKEISYDEM
210 220 230 240 250
LELANLGAGV LHPRAVEFAK NYEVPLEVRS SMENERGTMV KEEVSMEQHL
260 270 280 290 300
IVRGIAFEDQ VTRVTVVGIE KYLQSVATIF TALANRGINV DIIIQNATNS
310 320 330 340 350
ETASVSFSIR TEDLPETLQV LQALEGADVH YESGLAKVSI VGSGMISNPG
360 370 380 390 400
VAARVFEVLA DQGIEIKMVS ISEIKISTVI DEKYMVSAVE ELHEAFGLAE

EAAAVRS
Length:407
Mass (Da):44,002
Last modified:October 1, 1996 - v1
Checksum:i2A848E6A7037B172
GO
Isoform Beta (identifier: P53553-2) [UniParc]FASTAAdd to basket
Also known as: Aspartokinase 2 subunit beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-245: Missing.

Note: No experimental confirmation available.
Show »
Length:162
Mass (Da):17,435
Checksum:i3F8D01A6B3591229
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186541 – 245Missing in isoform Beta. CuratedAdd BLAST245

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L46351 Genomic DNA Translation: AAB06216.1
PIRiJC4640

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiAK_GEOSE
AccessioniPrimary (citable) accession number: P53553
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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