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Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi-1)
  3. ATP-dependent 6-phosphofructokinase (emp-3)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63Glyceraldehyde 3-phosphateBy similarity1
Active sitei112Proton donorBy similarity1
Metal bindingi113Zinc 1; catalyticBy similarity1
Metal bindingi147Zinc 2By similarity1
Metal bindingi177Zinc 2By similarity1
Metal bindingi229Zinc 1; catalyticBy similarity1
Binding sitei230Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
Metal bindingi268Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fba
ORF Names:NCU07807
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 2, Linkage Group V

Organism-specific databases

EuPathDBiFungiDB:NCU07807

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001787591 – 362Fructose-bisphosphate aldolaseAdd BLAST362

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP53444
SMRiP53444
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni269 – 271Dihydroxyacetone phosphate bindingBy similarity3
Regioni290 – 293Dihydroxyacetone phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000227794
InParanoidiP53444
KOiK01624
OMAiELCKDCI
OrthoDBiEOG092C385G

Family and domain databases

CDDicd00946 FBP_aldolase_IIA, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR000771 FBA_II
IPR006411 Fruct_bisP_bact
PANTHERiPTHR30559 PTHR30559, 1 hit
PfamiView protein in Pfam
PF01116 F_bP_aldolase, 1 hit
PIRSFiPIRSF001359 F_bP_aldolase_II, 1 hit
TIGRFAMsiTIGR00167 cbbA, 1 hit
TIGR01520 FruBisAldo_II_A, 1 hit
PROSITEiView protein in PROSITE
PS00602 ALDOLASE_CLASS_II_1, 1 hit
PS00806 ALDOLASE_CLASS_II_2, 1 hit

Sequencei

Sequence statusi: Complete.

P53444-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIFDELNLP AGVLYGDDVL KLFQYAREKQ FAIPACNVTS SSTAVAALEA
60 70 80 90 100
ARDQKAPIIL QTSQGGAAFF AGKGIKDSAE KREASVAGAI AAAHYIRSIA
110 120 130 140 150
PIYGIPVVLH TDHCAKKLLP WLDGMLEEDE KFFKANGVPL FSSHMIDLSE
160 170 180 190 200
EPVEENISTC VKYLKRMAPM KQWLEMEIGI TGGEEDGVDN SEVDNASLYT
210 220 230 240 250
QPEDIWQIEE AFRPISPYFS IAAGFGNVHG VYAPGNVKLH PELLGKHQAY
260 270 280 290 300
VSEKLGGKDK KPVFFVFHGG SGSSKEEYRE AISNGVVKVN VDTDLQWSYL
310 320 330 340 350
VGIRDYILNN IDYLRSQVGN PEGPNKPNKK KYDPRVWIRE GEKTMKARVE
360
EALKDFNAAG TV
Length:362
Mass (Da):39,889
Last modified:March 1, 2004 - v2
Checksum:iC402186EEEBD9368
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 15MGIFD…AGVLY → MRCPIPEHKQNLTFSLPL in AAB00930 (Ref. 1) CuratedAdd BLAST15
Sequence conflicti58 – 59II → SS in AAB00930 (Ref. 1) Curated2
Sequence conflicti66G → C in AAB00930 (Ref. 1) Curated1
Sequence conflicti82 – 84REA → ARD in AAB00930 (Ref. 1) Curated3
Sequence conflicti109L → RI in AAB00930 (Ref. 1) Curated1
Sequence conflicti120 – 126PWLDGML → FGDGIP in AAB00930 (Ref. 1) Curated7
Sequence conflicti211 – 212AF → DS in AAB00930 (Ref. 1) Curated2
Sequence conflicti222A → G in AAB00930 (Ref. 1) Curated1
Sequence conflicti242E → D in AAB00930 (Ref. 1) Curated1
Sequence conflicti247H → Q in AAB00930 (Ref. 1) Curated1
Sequence conflicti252S → P in AAB00930 (Ref. 1) Curated1
Sequence conflicti263V → F in AAB00930 (Ref. 1) Curated1
Sequence conflicti352 – 360ALKDFNAAG → GPQGPSNTTV in AAB00930 (Ref. 1) Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42380 Genomic DNA Translation: AAB00930.1
CM002240 Genomic DNA Translation: ESA42348.1
CM002240 Genomic DNA Translation: ESA42349.1
PIRiT47260
RefSeqiXP_011394728.1, XM_011396426.1
XP_011394729.1, XM_011396427.1

Genome annotation databases

EnsemblFungiiESA42348; ESA42348; NCU07807
ESA42349; ESA42349; NCU07807
GeneIDi3874540
KEGGincr:NCU07807

Similar proteinsi

Entry informationi

Entry nameiALF_NEUCR
AccessioniPrimary (citable) accession number: P53444
Secondary accession number(s): A7UX41, Q7S1C1, V5IKX9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 1, 2004
Last modified: December 20, 2017
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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