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Protein

N-glycosylase/DNA lyase

Gene

OGG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.

Miscellaneous

Present with 3690 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei134DNABy similarity1
Binding sitei139DNABy similarity1
Binding sitei189DNABy similarity1
Active sitei241Schiff-base intermediate with DNA1
Binding sitei2588-oxoguanine; via carbonyl oxygenBy similarity1
Binding sitei2608-oxoguanineBy similarity1
Binding sitei262DNABy similarity1
Binding sitei3208-oxoguanineBy similarity1
Binding sitei3248-oxoguanineBy similarity1

GO - Molecular functioni

GO - Biological processi

  • base-excision repair, AP site formation Source: SGD
  • DNA repair Source: SGD
  • error-free translesion synthesis Source: SGD
  • nucleotide-excision repair Source: InterPro
  • telomere maintenance via telomerase Source: SGD

Keywordsi

Molecular functionGlycosidase, Hydrolase, Lyase, Multifunctional enzyme
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-32655-MONOMER
ReactomeiR-SCE-110329 Cleavage of the damaged pyrimidine
R-SCE-110331 Cleavage of the damaged purine
R-SCE-110357 Displacement of DNA glycosylase by APEX1

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyase
Including the following 2 domains:
8-oxoguanine DNA glycosylase (EC:3.2.2.-)
DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
Short name:
AP lyase
Gene namesi
Name:OGG1
Ordered Locus Names:YML060W
ORF Names:YM9958.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML060W
SGDiS000004525 OGG1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi241K → Q: Abolishes both DNA glycosylase and AP lyase activity. 2 Publications1
Mutagenesisi241K → R: Diminishes both DNA glycosylase and AP lyase activity. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000585951 – 376N-glycosylase/DNA lyaseAdd BLAST376

Proteomic databases

MaxQBiP53397
PaxDbiP53397
PRIDEiP53397
TopDownProteomicsiP53397

PTM databases

CarbonylDBiP53397

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35106, 87 interactors
DIPiDIP-4538N
IntActiP53397, 8 interactors
MINTiP53397
STRINGi4932.YML060W

Structurei

3D structure databases

ProteinModelPortaliP53397
SMRiP53397
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the type-1 OGG1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00640000091554
HOGENOMiHOG000180756
InParanoidiP53397
KOiK03660
OMAiEYAGYAQ
OrthoDBiEOG092C29TV

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR003265 HhH-GPD_domain
IPR023170 HTH_base_excis_C
IPR004577 Ogg1
IPR012904 OGG_N
PfamiView protein in Pfam
PF00730 HhH-GPD, 1 hit
PF07934 OGG_N, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
TIGRFAMsiTIGR00588 ogg, 1 hit

Sequencei

Sequence statusi: Complete.

P53397-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYKFGKLAI NKSELCLANV LQAGQSFRWI WDEKLNQYST TMKIGQQEKY
60 70 80 90 100
SVVILRQDEE NEILEFVAVG DCGNQDALKT HLMKYFRLDV SLKHLFDNVW
110 120 130 140 150
IPSDKAFAKL SPQGIRILAQ EPWETLISFI CSSNNNISRI TRMCNSLCSN
160 170 180 190 200
FGNLITTIDG VAYHSFPTSE ELTSRATEAK LRELGFGYRA KYIIETARKL
210 220 230 240 250
VNDKAEANIT SDTTYLQSIC KDAQYEDVRE HLMSYNGVGP KVADCVCLMG
260 270 280 290 300
LHMDGIVPVD VHVSRIAKRD YQISANKNHL KELRTKYNAL PISRKKINLE
310 320 330 340 350
LDHIRLMLFK KWGSYAGWAQ GVLFSKEIGG TSGSTTTGTI KKRKWDMIKE
360 370
TEAIVTKQMK LKVELSDLHI KEAKID
Length:376
Mass (Da):42,781
Last modified:October 1, 1996 - v1
Checksum:i4ADB72EB20E4BF20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44855 Genomic DNA Translation: AAC49312.1
Z46729 Genomic DNA Translation: CAA86715.1
BK006946 Genomic DNA Translation: DAA09837.1
PIRiS49801
RefSeqiNP_013651.1, NM_001182419.1

Genome annotation databases

EnsemblFungiiYML060W; YML060W; YML060W
GeneIDi854942
KEGGisce:YML060W

Similar proteinsi

Entry informationi

Entry nameiOGG1_YEAST
AccessioniPrimary (citable) accession number: P53397
Secondary accession number(s): D6VZB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 18, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

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