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Protein

Cytosolic Fe-S cluster assembly factor NUBP1

Gene

NUBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery (PubMed:18573874). Required for maturation of extramitochondrial Fe-S proteins (PubMed:18573874). The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins (PubMed:18573874). Implicated in the regulation of centrosome duplication (By similarity). Negatively regulates cilium formation and structure (By similarity).By similarity1 Publication

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi22Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi25Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi31Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi235Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi238Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 69ATPUniRule annotation8

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-UniRule
  • ATP binding Source: UniProtKB-KW
  • iron-sulfur cluster binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • nucleotide binding Source: ProtInc

GO - Biological processi

Keywordsi

Biological processCilium biogenesis/degradation
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-2564830 Cytosolic iron-sulfur cluster assembly

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP1UniRule annotation
Alternative name(s):
Nucleotide-binding protein 1UniRule annotation
Short name:
NBP 1UniRule annotation
Gene namesi
Name:NUBP1UniRule annotation
Synonyms:NBP, NBP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000103274.10
HGNCiHGNC:8041 NUBP1
MIMi600280 gene
neXtProtiNX_P53384

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4682
OpenTargetsiENSG00000103274
PharmGKBiPA31823

Polymorphism and mutation databases

DMDMi257050984

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001849431 – 320Cytosolic Fe-S cluster assembly factor NUBP1Add BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei319PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP53384
MaxQBiP53384
PaxDbiP53384
PeptideAtlasiP53384
PRIDEiP53384
ProteomicsDBi56579
56580 [P53384-2]

PTM databases

iPTMnetiP53384
PhosphoSitePlusiP53384

Expressioni

Gene expression databases

BgeeiENSG00000103274 Expressed in 203 organ(s), highest expression level in right adrenal gland
CleanExiHS_NUBP1
ExpressionAtlasiP53384 baseline and differential
GenevisibleiP53384 HS

Organism-specific databases

HPAiHPA041656
HPA041799

Interactioni

Subunit structurei

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains (By similarity). Interacts with KIFC1 (By similarity). Interacts with NUBP2 (PubMed:18573874). Interacts with the BBS/CCT complex subunit CCT1 (By similarity).UniRule annotationBy similarity1 Publication

Protein-protein interaction databases

BioGridi110762, 53 interactors
IntActiP53384, 1 interactor
MINTiP53384
STRINGi9606.ENSP00000283027

Structurei

3D structure databases

ProteinModelPortaliP53384
SMRiP53384
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410KCSJ Eukaryota
COG0489 LUCA
GeneTreeiENSGT00390000009735
HOGENOMiHOG000079916
HOVERGENiHBG051027
InParanoidiP53384
OMAiHCPGVES
OrthoDBiEOG091G0DY5
PhylomeDBiP53384
TreeFamiTF300755

Family and domain databases

CDDicd02037 MRP-like, 1 hit
HAMAPiMF_02040 Mrp_NBP35, 1 hit
MF_03038 NUBP1, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR019591 Mrp/NBP35_ATP-bd
IPR000808 Mrp_CS
IPR028601 NUBP1/Nbp35
IPR027417 P-loop_NTPase
IPR033756 YlxH/NBP35
PANTHERiPTHR23264 PTHR23264, 1 hit
PfamiView protein in Pfam
PF10609 ParA, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS01215 MRP, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P53384-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEEVPHDCPG ADSAQAGRGA SCQGCPNQRL CASGAGATPD TAIEEIKEKM
60 70 80 90 100
KTVKHKILVL SGKGGVGKST FSAHLAHGLA EDENTQIALL DIDICGPSIP
110 120 130 140 150
KIMGLEGEQV HQSGSGWSPV YVEDNLGVMS VGFLLSSPDD AVIWRGPKKN
160 170 180 190 200
GMIKQFLRDV DWGEVDYLIV DTPPGTSDEH LSVVRYLATA HIDGAVIITT
210 220 230 240 250
PQEVSLQDVR KEINFCRKVK LPIIGVVENM SGFICPKCKK ESQIFPPTTG
260 270 280 290 300
GAELMCQDLE VPLLGRVPLD PLIGKNCDKG QSFFIDAPDS PATLAYRSII
310 320
QRIQEFCNLH QSKEENLISS
Length:320
Mass (Da):34,534
Last modified:September 1, 2009 - v2
Checksum:i346E1C694224E437
GO
Isoform 2 (identifier: P53384-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-120: Missing.

Note: No experimental confirmation available.
Show »
Length:309
Mass (Da):33,412
Checksum:i1A2201FF81478FFE
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
I3L3A0I3L3A0_HUMAN
Cytosolic Fe-S cluster assembly fac...
NUBP1
142Annotation score:
I3L531I3L531_HUMAN
Cytosolic Fe-S cluster assembly fac...
NUBP1
168Annotation score:
I3L518I3L518_HUMAN
Cytosolic Fe-S cluster assembly fac...
NUBP1
63Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti204V → L in AAA61932 (PubMed:7926816).Curated1
Sequence conflicti232G → P in AAA61932 (PubMed:7926816).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02035939P → A2 PublicationsCorresponds to variant dbSNP:rs2233531Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_029043110 – 120Missing in isoform 2. 1 PublicationAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01833 mRNA Translation: AAA61932.1
AK223204 mRNA Translation: BAD96924.1
BC100290 mRNA Translation: AAI00291.1
BC109322 mRNA Translation: AAI09323.1
BC109323 mRNA Translation: AAI09324.1
CCDSiCCDS10543.1 [P53384-1]
CCDS61839.1 [P53384-2]
PIRiJC4010
RefSeqiNP_001265435.1, NM_001278506.1 [P53384-2]
NP_002475.2, NM_002484.3 [P53384-1]
UniGeneiHs.81469

Genome annotation databases

EnsembliENST00000283027; ENSP00000283027; ENSG00000103274 [P53384-1]
ENST00000433392; ENSP00000409654; ENSG00000103274 [P53384-2]
GeneIDi4682
KEGGihsa:4682
UCSCiuc002daa.3 human [P53384-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01833 mRNA Translation: AAA61932.1
AK223204 mRNA Translation: BAD96924.1
BC100290 mRNA Translation: AAI00291.1
BC109322 mRNA Translation: AAI09323.1
BC109323 mRNA Translation: AAI09324.1
CCDSiCCDS10543.1 [P53384-1]
CCDS61839.1 [P53384-2]
PIRiJC4010
RefSeqiNP_001265435.1, NM_001278506.1 [P53384-2]
NP_002475.2, NM_002484.3 [P53384-1]
UniGeneiHs.81469

3D structure databases

ProteinModelPortaliP53384
SMRiP53384
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110762, 53 interactors
IntActiP53384, 1 interactor
MINTiP53384
STRINGi9606.ENSP00000283027

PTM databases

iPTMnetiP53384
PhosphoSitePlusiP53384

Polymorphism and mutation databases

DMDMi257050984

Proteomic databases

EPDiP53384
MaxQBiP53384
PaxDbiP53384
PeptideAtlasiP53384
PRIDEiP53384
ProteomicsDBi56579
56580 [P53384-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000283027; ENSP00000283027; ENSG00000103274 [P53384-1]
ENST00000433392; ENSP00000409654; ENSG00000103274 [P53384-2]
GeneIDi4682
KEGGihsa:4682
UCSCiuc002daa.3 human [P53384-1]

Organism-specific databases

CTDi4682
DisGeNETi4682
EuPathDBiHostDB:ENSG00000103274.10
GeneCardsiNUBP1
H-InvDBiHIX0038641
HGNCiHGNC:8041 NUBP1
HPAiHPA041656
HPA041799
MIMi600280 gene
neXtProtiNX_P53384
OpenTargetsiENSG00000103274
PharmGKBiPA31823
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410KCSJ Eukaryota
COG0489 LUCA
GeneTreeiENSGT00390000009735
HOGENOMiHOG000079916
HOVERGENiHBG051027
InParanoidiP53384
OMAiHCPGVES
OrthoDBiEOG091G0DY5
PhylomeDBiP53384
TreeFamiTF300755

Enzyme and pathway databases

ReactomeiR-HSA-2564830 Cytosolic iron-sulfur cluster assembly

Miscellaneous databases

GenomeRNAii4682
PROiPR:P53384
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103274 Expressed in 203 organ(s), highest expression level in right adrenal gland
CleanExiHS_NUBP1
ExpressionAtlasiP53384 baseline and differential
GenevisibleiP53384 HS

Family and domain databases

CDDicd02037 MRP-like, 1 hit
HAMAPiMF_02040 Mrp_NBP35, 1 hit
MF_03038 NUBP1, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR019591 Mrp/NBP35_ATP-bd
IPR000808 Mrp_CS
IPR028601 NUBP1/Nbp35
IPR027417 P-loop_NTPase
IPR033756 YlxH/NBP35
PANTHERiPTHR23264 PTHR23264, 1 hit
PfamiView protein in Pfam
PF10609 ParA, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS01215 MRP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNUBP1_HUMAN
AccessioniPrimary (citable) accession number: P53384
Secondary accession number(s): Q32M30, Q498A9, Q53FS7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 1, 2009
Last modified: November 7, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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