UniProtKB - P53368 (8ODP_MOUSE)
Protein
7,8-dihydro-8-oxoguanine triphosphatase
Gene
Nudt1
Organism
Mus musculus (Mouse)
Status
Functioni
Antimutagenic (PubMed:11572992). Plays a redundant role in sanitizing oxidized nucleotide pools, such as 8-oxo-dGTP pools (PubMed:29281266). Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP (By similarity). Does not play a role in U8 snoRNA decapping activity (PubMed:21070968). Binds U8 snoRNA (PubMed:21070968).By similarity1 Publication3 Publications
Catalytic activityi
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 8 | Substrate; via carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 23 | SubstrateCombined sources1 Publication | 1 | |
Sitei | 27 | Important for 2-OH-dATPase and 8-oxo-dGTPase activitiesBy similarity | 1 | |
Binding sitei | 33 | SubstrateCombined sources1 Publication | 1 | |
Metal bindingi | 37 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 52 | MagnesiumBy similarity | 1 | |
Metal bindingi | 55 | MagnesiumBy similarity | 1 | |
Metal bindingi | 56 | MagnesiumBy similarity | 1 | |
Sitei | 117 | Essential for 2-OH-dATPase and 8-oxo-dGTPase activitiesBy similarity | 1 | |
Sitei | 119 | Essential for 2-OH-dATPase activity and important for 8-oxo-dGTPase activityBy similarity | 1 |
GO - Molecular functioni
- 8-oxo-7,8-dihydrodeoxyguanosine triphosphate pyrophosphatase activity Source: UniProtKB
- 8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity Source: MGI
- ATP diphosphatase activity Source: UniProtKB
- dATP pyrophosphohydrolase activity Source: UniProtKB-EC
- GTPase activity Source: UniProtKB
- GTP diphosphatase activity Source: MGI
- metal ion binding Source: UniProtKB-KW
- snoRNA binding Source: UniProtKB
GO - Biological processi
- aging Source: Ensembl
- dATP catabolic process Source: UniProtKB
- dGTP catabolic process Source: MGI
- DNA protection Source: UniProtKB
- DNA repair Source: InterPro
- male gonad development Source: Ensembl
- purine nucleotide catabolic process Source: MGI
- response to cadmium ion Source: Ensembl
Keywordsi
Molecular function | Hydrolase, RNA-binding |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.6.1.56, 3474 |
Reactomei | R-MMU-2393930, Phosphate bond hydrolysis by NUDT proteins |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Nudt1 Synonyms:Mth1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109280, Nudt1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
- Nucleus membrane By similarity
Other locations
Cytosol
- cytosol Source: MGI
Extracellular region or secreted
- extracellular space Source: MGI
Mitochondrion
- mitochondrial matrix Source: UniProtKB
- mitochondrion Source: UniProtKB
Nucleus
- nuclear membrane Source: MGI
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- acrosomal vesicle Source: MGI
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
Mutant mice appear normal, but have higher incidence of tumors in lung, liver and stomach.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000057100 | 21 – 156 | 7,8-dihydro-8-oxoguanine triphosphataseAdd BLAST | 136 |
Proteomic databases
EPDi | P53368 |
PaxDbi | P53368 |
PRIDEi | P53368 |
PTM databases
iPTMneti | P53368 |
PhosphoSitePlusi | P53368 |
Expressioni
Tissue specificityi
High expression levels detected in thymus, liver, spleen, kidney, testis and large intestine, with lower levels detected in brain, heart, lung and stomach (at protein level). Expressed in kidney, liver and small intestine.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000036639, Expressed in cochlea and 256 other tissues |
Genevisiblei | P53368, MM |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000059983 |
Miscellaneous databases
RNActi | P53368, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P53368 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 132 | Nudix hydrolasePROSITE-ProRule annotationAdd BLAST | 112 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 35 – 38 | Substrate bindingCombined sources1 Publication | 4 | |
Regioni | 117 – 120 | Substrate bindingCombined sources1 Publication | 4 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 37 – 58 | Nudix boxPROSITE-ProRule annotationAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the Nudix hydrolase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502S254, Eukaryota |
GeneTreei | ENSGT00390000000341 |
InParanoidi | P53368 |
OMAi | VGYFKFQ |
PhylomeDBi | P53368 |
TreeFami | TF106348 |
Family and domain databases
InterProi | View protein in InterPro IPR020476, Nudix_hydrolase IPR015797, NUDIX_hydrolase-like_dom_sf IPR020084, NUDIX_hydrolase_CS IPR000086, NUDIX_hydrolase_dom IPR003563, OxG-triPHTase |
Pfami | View protein in Pfam PF00293, NUDIX, 1 hit |
PRINTSi | PR01403, 8OXTPHPHTASE PR00502, NUDIXFAMILY |
SUPFAMi | SSF55811, SSF55811, 1 hit |
PROSITEi | View protein in PROSITE PS51462, NUDIX, 1 hit PS00893, NUDIX_BOX, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P53368-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTSRLYTLV LVLQPQRVLL GMKKRGFGAG RWNGFGGKVQ EGETIEDGAK
60 70 80 90 100
RELLEESGLS VDTLHKVGHI SFEFVGSPEL MDVHIFSADH VHGTPTESEE
110 120 130 140 150
MRPQWFQLDQ IPFADLWPDD SYWFPLLLQK KKFCGHFKFQ DQDTILSYSL
REVDSF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YWH7 | D3YWH7_MOUSE | 7,8-dihydro-8-oxoguanine triphospha... | Nudt1 | 151 | Annotation score: | ||
A0A0G2JFE5 | A0A0G2JFE5_MOUSE | 7,8-dihydro-8-oxoguanine triphospha... | Nudt1 | 112 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 116 | L → M in AAH21940 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D49956 mRNA Translation: BAA08711.1 D88356 Genomic DNA Translation: BAA19866.1 AK011695 mRNA Translation: BAB27785.1 AK088309 mRNA Translation: BAC40274.1 AK168312 mRNA Translation: BAE40252.1 CH466529 Genomic DNA Translation: EDL19122.1 CH466529 Genomic DNA Translation: EDL19123.1 BC021940 mRNA Translation: AAH21940.1 BC098239 mRNA Translation: AAH98239.1 |
CCDSi | CCDS19818.1 |
PIRi | I49446 |
RefSeqi | NP_032663.1, NM_008637.1 XP_006504718.1, XM_006504655.2 XP_006504719.1, XM_006504656.2 XP_006504720.1, XM_006504657.2 |
Genome annotation databases
Ensembli | ENSMUST00000050205; ENSMUSP00000059983; ENSMUSG00000036639 ENSMUST00000071881; ENSMUSP00000071778; ENSMUSG00000036639 ENSMUST00000110826; ENSMUSP00000106450; ENSMUSG00000036639 ENSMUST00000110827; ENSMUSP00000106451; ENSMUSG00000036639 |
GeneIDi | 17766 |
KEGGi | mmu:17766 |
UCSCi | uc009ahn.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D49956 mRNA Translation: BAA08711.1 D88356 Genomic DNA Translation: BAA19866.1 AK011695 mRNA Translation: BAB27785.1 AK088309 mRNA Translation: BAC40274.1 AK168312 mRNA Translation: BAE40252.1 CH466529 Genomic DNA Translation: EDL19122.1 CH466529 Genomic DNA Translation: EDL19123.1 BC021940 mRNA Translation: AAH21940.1 BC098239 mRNA Translation: AAH98239.1 |
CCDSi | CCDS19818.1 |
PIRi | I49446 |
RefSeqi | NP_032663.1, NM_008637.1 XP_006504718.1, XM_006504655.2 XP_006504719.1, XM_006504656.2 XP_006504720.1, XM_006504657.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5MZE | X-ray | 2.10 | A/B/C/D | 1-156 | [»] | |
5MZG | X-ray | 1.85 | A/B | 1-156 | [»] | |
6EHH | X-ray | 2.40 | A/B/C/D | 1-156 | [»] | |
SMRi | P53368 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000059983 |
PTM databases
iPTMneti | P53368 |
PhosphoSitePlusi | P53368 |
Proteomic databases
EPDi | P53368 |
PaxDbi | P53368 |
PRIDEi | P53368 |
Protocols and materials databases
Antibodypediai | 1866, 227 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 4521 |
MGIi | MGI:109280, Nudt1 |
Phylogenomic databases
eggNOGi | ENOG502S254, Eukaryota |
GeneTreei | ENSGT00390000000341 |
InParanoidi | P53368 |
OMAi | VGYFKFQ |
PhylomeDBi | P53368 |
TreeFami | TF106348 |
Enzyme and pathway databases
BRENDAi | 3.6.1.56, 3474 |
Reactomei | R-MMU-2393930, Phosphate bond hydrolysis by NUDT proteins |
Miscellaneous databases
BioGRID-ORCSi | 17766, 1 hit in 17 CRISPR screens |
ChiTaRSi | Nudt1, mouse |
PROi | PR:P53368 |
RNActi | P53368, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036639, Expressed in cochlea and 256 other tissues |
Genevisiblei | P53368, MM |
Family and domain databases
InterProi | View protein in InterPro IPR020476, Nudix_hydrolase IPR015797, NUDIX_hydrolase-like_dom_sf IPR020084, NUDIX_hydrolase_CS IPR000086, NUDIX_hydrolase_dom IPR003563, OxG-triPHTase |
Pfami | View protein in Pfam PF00293, NUDIX, 1 hit |
PRINTSi | PR01403, 8OXTPHPHTASE PR00502, NUDIXFAMILY |
SUPFAMi | SSF55811, SSF55811, 1 hit |
PROSITEi | View protein in PROSITE PS51462, NUDIX, 1 hit PS00893, NUDIX_BOX, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 8ODP_MOUSE | |
Accessioni | P53368Primary (citable) accession number: P53368 Secondary accession number(s): P97795, Q542J4, Q8VDG0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families