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Protein

Arfaptin-2

Gene

ARFIP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • GTP binding Source: ProtInc
  • GTP-dependent protein binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • phosphatidylinositol-4-phosphate binding Source: FlyBase
  • phospholipid binding Source: GO_Central
  • protein domain specific binding Source: InterPro
  • Rac GTPase binding Source: UniProtKB

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-6811440 Retrograde transport at the Trans-Golgi-Network
SIGNORiP53365

Names & Taxonomyi

Protein namesi
Recommended name:
Arfaptin-2
Alternative name(s):
ADP-ribosylation factor-interacting protein 2
Partner of RAC1
Short name:
Protein POR1
Gene namesi
Name:ARFIP2
Synonyms:POR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000132254.12
HGNCiHGNC:17160 ARFIP2
MIMi601638 gene
neXtProtiNX_P53365

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi23647
OpenTargetsiENSG00000132254
PharmGKBiPA134879398

Polymorphism and mutation databases

BioMutaiARFIP2
DMDMi1703205

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000646671 – 341Arfaptin-2Add BLAST341

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72PhosphoserineCombined sources1
Modified residuei76PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP53365
MaxQBiP53365
PaxDbiP53365
PeptideAtlasiP53365
PRIDEiP53365
ProteomicsDBi56573
56574 [P53365-2]

PTM databases

iPTMnetiP53365
PhosphoSitePlusiP53365

Expressioni

Gene expression databases

BgeeiENSG00000132254 Expressed in 224 organ(s), highest expression level in prostate gland
CleanExiHS_ARFIP2
ExpressionAtlasiP53365 baseline and differential
GenevisibleiP53365 HS

Organism-specific databases

HPAiCAB032985
HPA038156

Interactioni

Subunit structurei

Interacts with RAC1 by binding directly to it. Specifically binds to GTP-bound ARF1 and ARF6, but binds to RAC1.GTP and RAC1.GDP with similar affinities. Directly interacts with ARL1 GTP-bound form; this interaction leads to a > 4-fold increase in the amount of ARL1-bound GTP at steady state level.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi117174, 43 interactors
IntActiP53365, 70 interactors
MINTiP53365
STRINGi9606.ENSP00000254584

Structurei

Secondary structure

1341
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP53365
SMRiP53365
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53365

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini121 – 321AHPROSITE-ProRule annotationAdd BLAST201

Phylogenomic databases

eggNOGiKOG3876 Eukaryota
ENOG410YJUM LUCA
GeneTreeiENSGT00500000044828
HOGENOMiHOG000236308
HOVERGENiHBG050564
InParanoidiP53365
KOiK20314
PhylomeDBiP53365
TreeFamiTF314945

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR010504 AH_dom
IPR030798 Arfaptin_fam
PANTHERiPTHR12141 PTHR12141, 1 hit
PfamiView protein in Pfam
PF06456 Arfaptin, 1 hit
SMARTiView protein in SMART
SM01015 Arfaptin, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
PROSITEiView protein in PROSITE
PS50870 AH, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P53365-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTDGILGKAA TMEIPIHGNG EARQLPEDDG LEQDLQQVMV SGPNLNETSI
60 70 80 90 100
VSGGYGGSGD GLIPTGSGRH PSHSTTPSGP GDEVARGIAG EKFDIVKKWG
110 120 130 140 150
INTYKCTKQL LSERFGRGSR TVDLELELQI ELLRETKRKY ESVLQLGRAL
160 170 180 190 200
TAHLYSLLQT QHALGDAFAD LSQKSPELQE EFGYNAETQK LLCKNGETLL
210 220 230 240 250
GAVNFFVSSI NTLVTKTMED TLMTVKQYEA ARLEYDAYRT DLEELSLGPR
260 270 280 290 300
DAGTRGRLES AQATFQAHRD KYEKLRGDVA IKLKFLEENK IKVMHKQLLL
310 320 330 340
FHNAVSAYFA GNQKQLEQTL QQFNIKLRPP GAEKPSWLEE Q
Length:341
Mass (Da):37,856
Last modified:October 1, 1996 - v1
Checksum:iAB8D224430D8E213
GO
Isoform 2 (identifier: P53365-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: MTDGILGKAA...VKKWGINTYK → MKPALCLVAMGALVMDSSPQ

Note: No experimental confirmation available.
Show »
Length:256
Mass (Da):29,044
Checksum:iE94956B55C3FFF70
GO
Isoform 3 (identifier: P53365-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Show »
Length:303
Mass (Da):33,782
Checksum:i6C52F76110467F97
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087X1E4A0A087X1E4_HUMAN
Arfaptin-2
ARFIP2
374Annotation score:
E9PRV8E9PRV8_HUMAN
Arfaptin-2
ARFIP2
202Annotation score:
E9PPY7E9PPY7_HUMAN
Arfaptin-2
ARFIP2
159Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225V → A in BAG63298 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0425241 – 105MTDGI…INTYK → MKPALCLVAMGALVMDSSPQ in isoform 2. 1 PublicationAdd BLAST105
Alternative sequenceiVSP_0469131 – 38Missing in isoform 3. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52522 mRNA Translation: AAA97924.1
AK301856 mRNA Translation: BAG63298.1
AK304512 mRNA Translation: BAG65318.1
AC084337 Genomic DNA No translation available.
CH471064 Genomic DNA Translation: EAW68707.1
CH471064 Genomic DNA Translation: EAW68708.1
BC000392 mRNA Translation: AAH00392.1
X97567 mRNA Translation: CAA66179.1
CCDSiCCDS55739.1 [P53365-2]
CCDS55740.1 [P53365-3]
CCDS7765.1 [P53365-1]
PIRiG02516
RefSeqiNP_001229784.1, NM_001242855.1 [P53365-3]
NP_001229785.1, NM_001242856.1 [P53365-2]
NP_036534.1, NM_012402.3 [P53365-1]
XP_005252897.1, XM_005252840.4 [P53365-1]
UniGeneiHs.732203
Hs.75139

Genome annotation databases

EnsembliENST00000254584; ENSP00000254584; ENSG00000132254 [P53365-1]
ENST00000396777; ENSP00000379998; ENSG00000132254 [P53365-1]
ENST00000423813; ENSP00000398375; ENSG00000132254 [P53365-3]
ENST00000445086; ENSP00000391427; ENSG00000132254 [P53365-2]
GeneIDi23647
KEGGihsa:23647
UCSCiuc001mdk.4 human [P53365-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52522 mRNA Translation: AAA97924.1
AK301856 mRNA Translation: BAG63298.1
AK304512 mRNA Translation: BAG65318.1
AC084337 Genomic DNA No translation available.
CH471064 Genomic DNA Translation: EAW68707.1
CH471064 Genomic DNA Translation: EAW68708.1
BC000392 mRNA Translation: AAH00392.1
X97567 mRNA Translation: CAA66179.1
CCDSiCCDS55739.1 [P53365-2]
CCDS55740.1 [P53365-3]
CCDS7765.1 [P53365-1]
PIRiG02516
RefSeqiNP_001229784.1, NM_001242855.1 [P53365-3]
NP_001229785.1, NM_001242856.1 [P53365-2]
NP_036534.1, NM_012402.3 [P53365-1]
XP_005252897.1, XM_005252840.4 [P53365-1]
UniGeneiHs.732203
Hs.75139

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I49X-ray2.80A/B118-341[»]
1I4DX-ray2.50A/B118-341[»]
1I4LX-ray2.70A/B118-341[»]
1I4TX-ray2.60A/B118-341[»]
4DCNX-ray3.01C/D118-315[»]
ProteinModelPortaliP53365
SMRiP53365
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117174, 43 interactors
IntActiP53365, 70 interactors
MINTiP53365
STRINGi9606.ENSP00000254584

PTM databases

iPTMnetiP53365
PhosphoSitePlusiP53365

Polymorphism and mutation databases

BioMutaiARFIP2
DMDMi1703205

Proteomic databases

EPDiP53365
MaxQBiP53365
PaxDbiP53365
PeptideAtlasiP53365
PRIDEiP53365
ProteomicsDBi56573
56574 [P53365-2]

Protocols and materials databases

DNASUi23647
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254584; ENSP00000254584; ENSG00000132254 [P53365-1]
ENST00000396777; ENSP00000379998; ENSG00000132254 [P53365-1]
ENST00000423813; ENSP00000398375; ENSG00000132254 [P53365-3]
ENST00000445086; ENSP00000391427; ENSG00000132254 [P53365-2]
GeneIDi23647
KEGGihsa:23647
UCSCiuc001mdk.4 human [P53365-1]

Organism-specific databases

CTDi23647
DisGeNETi23647
EuPathDBiHostDB:ENSG00000132254.12
GeneCardsiARFIP2
HGNCiHGNC:17160 ARFIP2
HPAiCAB032985
HPA038156
MIMi601638 gene
neXtProtiNX_P53365
OpenTargetsiENSG00000132254
PharmGKBiPA134879398
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3876 Eukaryota
ENOG410YJUM LUCA
GeneTreeiENSGT00500000044828
HOGENOMiHOG000236308
HOVERGENiHBG050564
InParanoidiP53365
KOiK20314
PhylomeDBiP53365
TreeFamiTF314945

Enzyme and pathway databases

ReactomeiR-HSA-6811440 Retrograde transport at the Trans-Golgi-Network
SIGNORiP53365

Miscellaneous databases

ChiTaRSiARFIP2 human
EvolutionaryTraceiP53365
GeneWikiiARFIP2
GenomeRNAii23647
PROiPR:P53365
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132254 Expressed in 224 organ(s), highest expression level in prostate gland
CleanExiHS_ARFIP2
ExpressionAtlasiP53365 baseline and differential
GenevisibleiP53365 HS

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR010504 AH_dom
IPR030798 Arfaptin_fam
PANTHERiPTHR12141 PTHR12141, 1 hit
PfamiView protein in Pfam
PF06456 Arfaptin, 1 hit
SMARTiView protein in SMART
SM01015 Arfaptin, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
PROSITEiView protein in PROSITE
PS50870 AH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARFP2_HUMAN
AccessioniPrimary (citable) accession number: P53365
Secondary accession number(s): B4DX86, B4E306, D3DQT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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