Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - P53355 (DAPK1_HUMAN)

Protein

Death-associated protein kinase 1

Gene

DAPK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.
Isoform 2 cannot induce apoptosis but can induce membrane blebbing.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+

Activity regulationi

Activated by Ca2+/calmodulin. Regulated by a locking mechanism, involving autophosphorylation at Ser-308 and calmodulin binding. In the inactive state, Ser-308 is phosphorylated. Activation involves its dephosphorylation and a release-of-autoinhibition mechanism where binding of calmodulin induces a conformational change that relieves the steric block of the active site by the autoinhibitory domain. Activity is modulated by UNC5B and NTN1. UNC5B activates it by inhibiting the phosphorylation at Ser-308, whereas NTN1 inhibits UNC5B-mediated activation of DAPK1. Endoplasmic-stress activates by causing Ser-308 dephosphorylation.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42ATP1
Binding sitei100ATP1
Active sitei139Proton acceptorPROSITE-ProRule annotation1
Binding sitei161ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 27ATP9
Nucleotide bindingi94 – 96ATP3

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • calmodulin binding Source: UniProtKB
  • calmodulin-dependent protein kinase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • protein kinase activity Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB
  • syntaxin-1 binding Source: UniProtKB
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionCalmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis, Translation regulation
LigandATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 2681
ReactomeiR-HSA-418889 via Dependence Receptors in the absence of ligand
SignaLinkiP53355
SIGNORiP53355

Names & Taxonomyi

Protein namesi
Recommended name:
Death-associated protein kinase 1 (EC:2.7.11.1)
Short name:
DAP kinase 1
Gene namesi
Name:DAPK1
Synonyms:DAPK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000196730.12
HGNCiHGNC:2674 DAPK1
MIMi600831 gene
neXtProtiNX_P53355

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42K → A: Loss of activity, apoptotic function and of autophosphorylation. 2 Publications1
Mutagenesisi289S → A: Loss of phosphorylation and significant increase in proapoptotic activity. 1
Mutagenesisi289S → E: Reduction in proapoptotic activity. 1
Mutagenesisi308S → A: Elevated Ca(2+)-calmodulin binding and Ca(2+)-calmodulin-independent kinase activity. Increases apoptotic activity. 1 Publication1
Mutagenesisi308S → D: Reduced Ca(2+)-calmodulin binding and Ca(2+)-calmodulin-independent kinase activity. Decreases apoptotic activity. 1 Publication1
Mutagenesisi313S → A: Minimal effect on activity. 1 Publication1

Organism-specific databases

DisGeNETi1612
OpenTargetsiENSG00000196730
PharmGKBiPA27142

Chemistry databases

ChEMBLiCHEMBL2558
DrugBankiDB04395 Phosphoaminophosphonic Acid-Adenylate Ester
GuidetoPHARMACOLOGYi2002

Polymorphism and mutation databases

BioMutaiDAPK1
DMDMi317373595

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859101 – 1430Death-associated protein kinase 1Add BLAST1430

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei289Phosphoserine; by RPS6KA1 and RPS6KA31 Publication1
Modified residuei308Phosphoserine; by autocatalysis3 Publications1
Modified residuei319PhosphoserineCombined sources1
Modified residuei333PhosphoserineCombined sources1
Modified residuei734Phosphoserine; by MAPK11 Publication1
Modified residuei1115PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex, leading to its degradation by the proteasome.1 Publication
Removal of the C-terminal tail of isoform 2 (corresponding to amino acids 296-337 of isoform 2) by proteolytic cleavage stimulates maximally its membrane-blebbing function.1 Publication
In response to mitogenic stimulation (PMA or EGF), phosphorylated at Ser-289; phosphorylation suppresses DAPK1 pro-apoptotic function. Autophosphorylation at Ser-308 inhibits its catalytic activity. Phosphorylation at Ser-734 by MAPK1 increases its catalytic activity and promotes cytoplasmic retention of MAPK1. Endoplasmic-stress can cause dephosphorylation at Ser-308.5 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP53355
MaxQBiP53355
PaxDbiP53355
PeptideAtlasiP53355
PRIDEiP53355
ProteomicsDBi56570
56571 [P53355-2]
56572 [P53355-3]

PTM databases

iPTMnetiP53355
PhosphoSitePlusiP53355

Expressioni

Tissue specificityi

Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.1 Publication

Inductioni

Up-regulated following treatment with IFNG/IFN-gamma.1 Publication

Gene expression databases

BgeeiENSG00000196730 Expressed in 212 organ(s), highest expression level in amniotic fluid
CleanExiHS_DAPK1
ExpressionAtlasiP53355 baseline and differential
GenevisibleiP53355 HS

Organism-specific databases

HPAiCAB037302
HPA040472
HPA048436

Interactioni

Subunit structurei

Interacts with KLHL20. Interacts (via death domain) with MAPK1 and MAPK3. Interacts with MAP1B (via N-terminus). Interacts (via death domain) with UNC5B (via death domain). Interacts with PRKD1 in an oxidative stress-regulated manner. Interacts with PIN1, PDCD6, BECN1, GRINB, TSC2 and STX1A. Interacts (via kinase domain) with DAPK3 (via kinase domain).15 Publications

Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi107982, 52 interactors
ComplexPortaliCPX-102 DAPK1 - calmodulin complex
IntActiP53355, 119 interactors
MINTiP53355
STRINGi9606.ENSP00000350785

Chemistry databases

BindingDBiP53355

Structurei

Secondary structure

11430
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP53355
SMRiP53355
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53355

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 275Protein kinasePROSITE-ProRule annotationAdd BLAST263
Repeati378 – 407ANK 1Add BLAST30
Repeati411 – 440ANK 2Add BLAST30
Repeati444 – 473ANK 3Add BLAST30
Repeati477 – 506ANK 4Add BLAST30
Repeati510 – 539ANK 5Add BLAST30
Repeati543 – 572ANK 6Add BLAST30
Repeati576 – 605ANK 7Add BLAST30
Repeati609 – 638ANK 8Add BLAST30
Domaini681 – 955RocPROSITE-ProRule annotationAdd BLAST275
Repeati875 – 904ANK 9Add BLAST30
Repeati1162 – 1196ANK 10Add BLAST35
Domaini1312 – 1396DeathPROSITE-ProRule annotationAdd BLAST85

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni267 – 334Calmodulin-bindingAdd BLAST68
Regioni292 – 301Autoinhibitory domainBy similarity10

Domaini

The autoinhibitory domain sterically blocks the substrate peptide-binding site by making both hydrophobic and electrostatic contacts with the kinase core.

Sequence similaritiesi

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.Curated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0032 Eukaryota
ENOG410XRMJ LUCA
GeneTreeiENSGT00760000118877
HOGENOMiHOG000082489
HOVERGENiHBG051296
InParanoidiP53355
KOiK08803
OMAiCQMEVIK
OrthoDBiEOG091G0J2O
PhylomeDBiP53355
TreeFamiTF314166

Family and domain databases

CDDicd00204 ANK, 3 hits
Gene3Di1.25.40.20, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR020676 DAPK1
IPR011029 DEATH-like_dom_sf
IPR000488 Death_domain
IPR011009 Kinase-like_dom_sf
IPR027417 P-loop_NTPase
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR020859 ROC_dom
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR44619 PTHR44619, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF00531 Death, 1 hit
PF00069 Pkinase, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 9 hits
SM00005 DEATH, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF47986 SSF47986, 1 hit
SSF48403 SSF48403, 1 hit
SSF52540 SSF52540, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 7 hits
PS50017 DEATH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS51424 ROC, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: P53355-1) [UniParc]FASTAAdd to basket
Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTVFRQENVD DYYDTGEELG SGQFAVVKKC REKSTGLQYA AKFIKKRRTK 
        60         70         80         90        100
SSRRGVSRED IEREVSILKE IQHPNVITLH EVYENKTDVI LILELVAGGE 
       110        120        130        140        150
LFDFLAEKES LTEEEATEFL KQILNGVYYL HSLQIAHFDL KPENIMLLDR 
       160        170        180        190        200
NVPKPRIKII DFGLAHKIDF GNEFKNIFGT PEFVAPEIVN YEPLGLEADM 
       210        220        230        240        250
WSIGVITYIL LSGASPFLGD TKQETLANVS AVNYEFEDEY FSNTSALAKD 
       260        270        280        290        300
FIRRLLVKDP KKRMTIQDSL QHPWIKPKDT QQALSRKASA VNMEKFKKFA 
       310        320        330        340        350
ARKKWKQSVR LISLCQRLSR SFLSRSNMSV ARSDDTLDEE DSFVMKAIIH 
       360        370        380        390        400
AINDDNVPGL QHLLGSLSNY DVNQPNKHGT PPLLIAAGCG NIQILQLLIK 
       410        420        430        440        450
RGSRIDVQDK GGSNAVYWAA RHGHVDTLKF LSENKCPLDV KDKSGEMALH 
       460        470        480        490        500
VAARYGHADV AQLLCSFGSN PNIQDKEEET PLHCAAWHGY YSVAKALCEA 
       510        520        530        540        550
GCNVNIKNRE GETPLLTASA RGYHDIVECL AEHGADLNAC DKDGHIALHL 
       560        570        580        590        600
AVRRCQMEVI KTLLSQGCFV DYQDRHGNTP LHVACKDGNM PIVVALCEAN 
       610        620        630        640        650
CNLDISNKYG RTPLHLAANN GILDVVRYLC LMGASVEALT TDGKTAEDLA 
       660        670        680        690        700
RSEQHEHVAG LLARLRKDTH RGLFIQQLRP TQNLQPRIKL KLFGHSGSGK 
       710        720        730        740        750
TTLVESLKCG LLRSFFRRRR PRLSSTNSSR FPPSPLASKP TVSVSINNLY 
       760        770        780        790        800
PGCENVSVRS RSMMFEPGLT KGMLEVFVAP THHPHCSADD QSTKAIDIQN 
       810        820        830        840        850
AYLNGVGDFS VWEFSGNPVY FCCYDYFAAN DPTSIHVVVF SLEEPYEIQL 
       860        870        880        890        900
NQVIFWLSFL KSLVPVEEPI AFGGKLKNPL QVVLVATHAD IMNVPRPAGG 
       910        920        930        940        950
EFGYDKDTSL LKEIRNRFGN DLHISNKLFV LDAGASGSKD MKVLRNHLQE 
       960        970        980        990       1000
IRSQIVSVCP PMTHLCEKII STLPSWRKLN GPNQLMSLQQ FVYDVQDQLN 
      1010       1020       1030       1040       1050
PLASEEDLRR IAQQLHSTGE INIMQSETVQ DVLLLDPRWL CTNVLGKLLS 
      1060       1070       1080       1090       1100
VETPRALHHY RGRYTVEDIQ RLVPDSDVEE LLQILDAMDI CARDLSSGTM 
      1110       1120       1130       1140       1150
VDVPALIKTD NLHRSWADEE DEVMVYGGVR IVPVEHLTPF PCGIFHKVQV 
      1160       1170       1180       1190       1200
NLCRWIHQQS TEGDADIRLW VNGCKLANRG AELLVLLVNH GQGIEVQVRG 
      1210       1220       1230       1240       1250
LETEKIKCCL LLDSVCSTIE NVMATTLPGL LTVKHYLSPQ QLREHHEPVM 
      1260       1270       1280       1290       1300
IYQPRDFFRA QTLKETSLTN TMGGYKESFS SIMCFGCHDV YSQASLGMDI 
      1310       1320       1330       1340       1350
HASDLNLLTR RKLSRLLDPP DPLGKDWCLL AMNLGLPDLV AKYNTSNGAP 
      1360       1370       1380       1390       1400
KDFLPSPLHA LLREWTTYPE STVGTLMSKL RELGRRDAAD FLLKASSVFK 
      1410       1420       1430
INLDGNGQEA YASSCNSGTS YNSISSVVSR
Length:1,430
Mass (Da):160,046
Last modified:January 11, 2011 - v6
Checksum:iE2C4246E7C78A6D2
GO
Isoform 2 (identifier: P53355-2) [UniParc]FASTAAdd to basket
Also known as: s-DAPK-1

The sequence of this isoform differs from the canonical sequence as follows:
     1-446: Missing.
     742-783: VSVSINNLYP...LEVFVAPTHH → GRNLHAGPVS...SLGLYWTLWP
     784-1430: Missing.

Show »
Length:337
Mass (Da):36,765
Checksum:i3BA58BA79D87E0C6
GO
Isoform 3 (identifier: P53355-3) [UniParc]FASTAAdd to basket
Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     1430-1430: R → RRNSHVWNPTV

Show »
Length:1,440
Mass (Da):161,237
Checksum:iD43BB6B6A3F0ABF9
GO
Isoform 4 (identifier: P53355-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     805-870: Missing.

Note: No experimental confirmation available.
Show »
Length:1,364
Mass (Da):152,467
Checksum:i60E285E6A32348CB
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WCQ3F8WCQ3_HUMAN
Death-associated protein kinase 1
DAPK1
188Annotation score:

Sequence cautioni

The sequence AAP35581 differs from that shown. Contaminating sequence. Sequence of unknown origin in the C-terminal part.Curated
The sequence CAA53712 differs from that shown. Reason: Frameshift at positions 462 and 464.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti490Y → H in BAC87163 (PubMed:14702039).Curated1
Sequence conflicti1217S → G in CAH18690 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_033235416V → I1 PublicationCorresponds to variant dbSNP:rs12343465Ensembl.1
Natural variantiVAR_040420461A → S1 Publication1
Natural variantiVAR_040421519S → A1 Publication1
Natural variantiVAR_040422540C → Y1 PublicationCorresponds to variant dbSNP:rs56327474Ensembl.1
Natural variantiVAR_060693591P → L1 PublicationCorresponds to variant dbSNP:rs36214022Ensembl.1
Natural variantiVAR_060694622I → M1 PublicationCorresponds to variant dbSNP:rs36215047Ensembl.1
Natural variantiVAR_040423941M → T1 Publication1
Natural variantiVAR_040424977R → W1 Publication1
Natural variantiVAR_040425978K → N1 Publication1
Natural variantiVAR_040426993Y → C1 Publication1
Natural variantiVAR_040427994D → E1 Publication1
Natural variantiVAR_0404281005E → Q1 Publication1
Natural variantiVAR_0404291007D → Y1 Publication1
Natural variantiVAR_0404301008L → P1 Publication1
Natural variantiVAR_0404311010R → C1 PublicationCorresponds to variant dbSNP:rs371784492Ensembl.1
Natural variantiVAR_0404321018T → A1 Publication1
Natural variantiVAR_0404331272M → I1 PublicationCorresponds to variant dbSNP:rs56169226Ensembl.1
Natural variantiVAR_0404341346S → N5 PublicationsCorresponds to variant dbSNP:rs1056719Ensembl.1
Natural variantiVAR_0404351405G → V2 PublicationsCorresponds to variant dbSNP:rs36220450Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0420531 – 446Missing in isoform 2. 1 PublicationAdd BLAST446
Alternative sequenceiVSP_042054742 – 783VSVSI…APTHH → GRNLHAGPVSPAGVGFRTLS FQGLGGKGVVFGSLGLYWTL WP in isoform 2. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_042055784 – 1430Missing in isoform 2. 1 PublicationAdd BLAST647
Alternative sequenceiVSP_054478805 – 870Missing in isoform 4. 1 PublicationAdd BLAST66
Alternative sequenceiVSP_0420561430R → RRNSHVWNPTV in isoform 3. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76104 mRNA Translation: CAA53712.1 Frameshift.
AK127855 mRNA Translation: BAC87163.1
CR749834 mRNA Translation: CAH18690.1
DQ436495 Genomic DNA Translation: ABD96827.1
AL160279 Genomic DNA No translation available.
AL161787 Genomic DNA No translation available.
AL591852 Genomic DNA No translation available.
CH471089 Genomic DNA Translation: EAW62727.1
BC113660 mRNA Translation: AAI13661.1
BC143733 mRNA Translation: AAI43734.1
BC143759 mRNA Translation: AAI43760.1
BT006935 mRNA Translation: AAP35581.1 Sequence problems.
CCDSiCCDS43842.1 [P53355-1]
PIRiI37275
RefSeqiNP_001275658.1, NM_001288729.1 [P53355-1]
NP_001275659.1, NM_001288730.1 [P53355-1]
NP_001275660.1, NM_001288731.1 [P53355-1]
NP_004929.2, NM_004938.3 [P53355-1]
UniGeneiHs.380277
Hs.693441

Genome annotation databases

EnsembliENST00000358077; ENSP00000350785; ENSG00000196730 [P53355-1]
ENST00000408954; ENSP00000386135; ENSG00000196730 [P53355-1]
ENST00000469640; ENSP00000418885; ENSG00000196730 [P53355-4]
ENST00000472284; ENSP00000417076; ENSG00000196730 [P53355-1]
ENST00000491893; ENSP00000419026; ENSG00000196730 [P53355-4]
ENST00000622514; ENSP00000484267; ENSG00000196730 [P53355-1]
GeneIDi1612
KEGGihsa:1612
UCSCiuc004apc.5 human [P53355-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs
Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76104 mRNA Translation: CAA53712.1 Frameshift.
AK127855 mRNA Translation: BAC87163.1
CR749834 mRNA Translation: CAH18690.1
DQ436495 Genomic DNA Translation: ABD96827.1
AL160279 Genomic DNA No translation available.
AL161787 Genomic DNA No translation available.
AL591852 Genomic DNA No translation available.
CH471089 Genomic DNA Translation: EAW62727.1
BC113660 mRNA Translation: AAI13661.1
BC143733 mRNA Translation: AAI43734.1
BC143759 mRNA Translation: AAI43760.1
BT006935 mRNA Translation: AAP35581.1 Sequence problems.
CCDSiCCDS43842.1 [P53355-1]
PIRiI37275
RefSeqiNP_001275658.1, NM_001288729.1 [P53355-1]
NP_001275659.1, NM_001288730.1 [P53355-1]
NP_001275660.1, NM_001288731.1 [P53355-1]
NP_004929.2, NM_004938.3 [P53355-1]
UniGeneiHs.380277
Hs.693441

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IG1X-ray1.80A2-285[»]
1JKKX-ray2.40A2-285[»]
1JKLX-ray1.62A2-285[»]
1JKSX-ray1.50A2-285[»]
1JKTX-ray3.50A/B2-285[»]
1P4FX-ray1.90A2-285[»]
1WVWX-ray2.40A1-278[»]
1WVXX-ray2.60A1-278[»]
1WVYX-ray2.80A1-278[»]
1YR5X-ray1.70B302-320[»]
2W4JX-ray1.30A1-277[»]
2W4KX-ray1.90A1-302[»]
2X0GX-ray2.20A1-334[»]
2XUUX-ray1.80A1-334[»]
2XZSX-ray2.00A/B2-312[»]
2Y0AX-ray2.60A2-304[»]
2Y4PX-ray2.65A/B/C/D1-285[»]
2Y4VX-ray1.80B302-320[»]
2YAKX-ray2.20A1-285[»]
3DFCX-ray1.90B1-285[»]
3DGKX-ray1.70A1-285[»]
3EH9X-ray1.70A2-285[»]
3EHAX-ray1.60A2-285[»]
3F5GX-ray1.85A2-285[»]
3F5UX-ray2.00A1-285[»]
3GU4X-ray1.35A1-285[»]
3GU5X-ray1.65A1-285[»]
3GU6X-ray1.49A1-285[»]
3GU7X-ray1.90A1-285[»]
3GU8X-ray1.60A1-285[»]
3GUBX-ray1.71A1-285[»]
3ZXTX-ray2.65A/B/C/D1-285[»]
4B4LX-ray1.75A1-334[»]
4PF4X-ray1.13A1-277[»]
4TL0X-ray2.70A1-334[»]
4TXCX-ray1.95A1-285[»]
4UV0X-ray2.49A1-321[»]
4YO4X-ray1.60A2-285[»]
4YPDX-ray1.40A2-285[»]
5AUTX-ray1.70A1-285[»]
5AUUX-ray1.70A1-285[»]
5AUVX-ray1.50A1-285[»]
5AUWX-ray1.50A1-285[»]
5AUXX-ray1.50A1-285[»]
5AUYX-ray2.00A1-285[»]
5AUZX-ray1.60A1-285[»]
5AV0X-ray1.85A1-285[»]
5AV1X-ray1.50A1-285[»]
5AV2X-ray1.50A1-285[»]
5AV3X-ray1.90A1-285[»]
5AV4X-ray1.40A1-285[»]
ProteinModelPortaliP53355
SMRiP53355
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107982, 52 interactors
ComplexPortaliCPX-102 DAPK1 - calmodulin complex
IntActiP53355, 119 interactors
MINTiP53355
STRINGi9606.ENSP00000350785

Chemistry databases

BindingDBiP53355
ChEMBLiCHEMBL2558
DrugBankiDB04395 Phosphoaminophosphonic Acid-Adenylate Ester
GuidetoPHARMACOLOGYi2002

PTM databases

iPTMnetiP53355
PhosphoSitePlusiP53355

Polymorphism and mutation databases

BioMutaiDAPK1
DMDMi317373595

Proteomic databases

EPDiP53355
MaxQBiP53355
PaxDbiP53355
PeptideAtlasiP53355
PRIDEiP53355
ProteomicsDBi56570
56571 [P53355-2]
56572 [P53355-3]

Protocols and materials databases

DNASUi1612
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358077; ENSP00000350785; ENSG00000196730 [P53355-1]
ENST00000408954; ENSP00000386135; ENSG00000196730 [P53355-1]
ENST00000469640; ENSP00000418885; ENSG00000196730 [P53355-4]
ENST00000472284; ENSP00000417076; ENSG00000196730 [P53355-1]
ENST00000491893; ENSP00000419026; ENSG00000196730 [P53355-4]
ENST00000622514; ENSP00000484267; ENSG00000196730 [P53355-1]
GeneIDi1612
KEGGihsa:1612
UCSCiuc004apc.5 human [P53355-1]

Organism-specific databases

CTDi1612
DisGeNETi1612
EuPathDBiHostDB:ENSG00000196730.12
GeneCardsiDAPK1
HGNCiHGNC:2674 DAPK1
HPAiCAB037302
HPA040472
HPA048436
MIMi600831 gene
neXtProtiNX_P53355
OpenTargetsiENSG00000196730
PharmGKBiPA27142
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0032 Eukaryota
ENOG410XRMJ LUCA
GeneTreeiENSGT00760000118877
HOGENOMiHOG000082489
HOVERGENiHBG051296
InParanoidiP53355
KOiK08803
OMAiCQMEVIK
OrthoDBiEOG091G0J2O
PhylomeDBiP53355
TreeFamiTF314166

Enzyme and pathway databases

BRENDAi2.7.11.1 2681
ReactomeiR-HSA-418889 via Dependence Receptors in the absence of ligand
SignaLinkiP53355
SIGNORiP53355

Miscellaneous databases

ChiTaRSiDAPK1 human
EvolutionaryTraceiP53355
GeneWikiiDAPK1
GenomeRNAii1612
PROiPR:P53355
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000196730 Expressed in 212 organ(s), highest expression level in amniotic fluid
CleanExiHS_DAPK1
ExpressionAtlasiP53355 baseline and differential
GenevisibleiP53355 HS

Family and domain databases

CDDicd00204 ANK, 3 hits
Gene3Di1.25.40.20, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR020676 DAPK1
IPR011029 DEATH-like_dom_sf
IPR000488 Death_domain
IPR011009 Kinase-like_dom_sf
IPR027417 P-loop_NTPase
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR020859 ROC_dom
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR44619 PTHR44619, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF00531 Death, 1 hit
PF00069 Pkinase, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 9 hits
SM00005 DEATH, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF47986 SSF47986, 1 hit
SSF48403 SSF48403, 1 hit
SSF52540 SSF52540, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 7 hits
PS50017 DEATH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS51424 ROC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDAPK1_HUMAN
AccessioniPrimary (citable) accession number: P53355
Secondary accession number(s): B7ZLD2
, B7ZLE7, Q14CQ7, Q1W5W0, Q68CP8, Q6ZRZ3, Q9BTL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 11, 2011
Last modified: October 10, 2018
This is version 202 of the entry and version 6 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  7. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again