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UniProtKB - P53283 (TPO2_YEAST)
Protein
Polyamine transporter 2
Gene
TPO2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Recognizes spermine, but not spermidine.
2 PublicationsGO - Molecular functioni
- antiporter activity Source: UniProtKB-KW
- spermine transmembrane transporter activity Source: SGD
- xenobiotic transmembrane transporter activity Source: GO_Central
GO - Biological processi
- spermine transport Source: SGD
- xenobiotic detoxification by transmembrane export across the plasma membrane Source: GO_Central
Keywordsi
Biological process | Antiport, Transport |
Protein family/group databases
TCDBi | 2.A.1.2.67, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Polyamine transporter 2 |
Gene namesi | Name:TPO2 Ordered Locus Names:YGR138C ORF Names:G6417 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003370, TPO2 |
VEuPathDBi | FungiDB:YGR138C |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications
Plasma Membrane
- plasma membrane Source: SGD
Vacuole
- fungal-type vacuole membrane Source: SGD
Other locations
- cell periphery Source: SGD
- integral component of membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 173 | CytoplasmicSequence analysisAdd BLAST | 173 | |
Transmembranei | 174 – 194 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 195 – 206 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 207 – 227 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 228 – 236 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 237 – 257 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 258 – 266 | ExtracellularSequence analysis | 9 | |
Transmembranei | 267 – 287 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 288 – 297 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 298 – 318 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 319 – 326 | ExtracellularSequence analysis | 8 | |
Transmembranei | 327 – 347 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 348 – 407 | CytoplasmicSequence analysisAdd BLAST | 60 | |
Transmembranei | 408 – 428 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 429 – 437 | ExtracellularSequence analysis | 9 | |
Transmembranei | 438 – 458 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 459 – 478 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 479 – 499 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 500 – 503 | ExtracellularSequence analysis | 4 | |
Transmembranei | 504 – 524 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 525 – 541 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 542 – 562 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 563 – 574 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 575 – 595 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 596 – 614 | CytoplasmicSequence analysisAdd BLAST | 19 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173439 | 1 – 614 | Polyamine transporter 2Add BLAST | 614 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 50 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P53283 |
PaxDbi | P53283 |
PRIDEi | P53283 |
PTM databases
iPTMneti | P53283 |
Expressioni
Inductioni
By transcription factor HAA1 in response to acetaldehyde accumulation.1 Publication
Interactioni
Binary interactionsi
P53283
With | #Exp. | IntAct |
---|---|---|
TPO3 [Q06451] | 3 | EBI-2044753,EBI-34275 |
Protein-protein interaction databases
BioGRIDi | 33385, 66 interactors |
DIPi | DIP-7902N |
IntActi | P53283, 26 interactors |
MINTi | P53283 |
STRINGi | 4932.YGR138C |
Miscellaneous databases
RNActi | P53283, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 40 | DisorderedSequence analysisAdd BLAST | 40 | |
Regioni | 99 – 144 | DisorderedSequence analysisAdd BLAST | 46 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 99 – 122 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 126 – 144 | Acidic residuesSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
Belongs to the major facilitator superfamily. DHA1 family. Polyamines/proton antiporter (TC 2.A.1.2.16) subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0255, Eukaryota |
GeneTreei | ENSGT00940000176486 |
HOGENOMi | CLU_008455_11_5_1 |
InParanoidi | P53283 |
OMAi | TWKEADP |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
i Sequence
Sequence statusi: Complete.
P53283-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDQESVVSF NSQNTSMVDV EGQQPQQYVP SKTNSRANQL KLTKTETVKS
60 70 80 90 100
LQDLGVTSAA PVPDINAPQT AKNNIFPEEY TMETPSGLVP VATLQSMGRT
110 120 130 140 150
ASALSRTRTK QLNRTATNSS STGKEEMEEE ETEEREDQSG ENELDPEIEF
160 170 180 190 200
VTFVTGDPEN PHNWPSWVRW SYTVLLSILV ICVAYGSACI SGGLGTVEKK
210 220 230 240 250
YHVGMEAAIL SCSLMVIGFS LGPLIWSPVS DLYGRRVAYF VSMGLYVIFN
260 270 280 290 300
IPCALAPNLG CLLACRFLCG VWSSSGLCLV GGSIADMFPS ETRGKAIAFF
310 320 330 340 350
AFAPYVGPVV GPLVNGFISV STGRMDLIFW VNMAFAGVMW IISSAIPETY
360 370 380 390 400
APVILKRKAA RLRKETGNPK IMTEQEAQGV SMSEMMRACL LRPLYFAVTE
410 420 430 440 450
PVLVATCFYV CLIYSLLYAF FFAFPVIFGE LYGYKDNLVG LMFIPIVIGA
460 470 480 490 500
LWALATTFYC ENKYLQIVKQ RKPTPEDRLL GAKIGAPFAA IALWILGATA
510 520 530 540 550
YKHIIWVGPA SAGLAFGFGM VLIYYSLNNY IIDCYVQYAS SALATKVFLR
560 570 580 590 600
SAGGAAFPLF TIQMYHKLNL HWGSWLLAFI STAMIALPFA FSYWGKGLRH
610
KLSKKDYSID SVEM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z72923 Genomic DNA Translation: CAA97151.1 BK006941 Genomic DNA Translation: DAA08230.1 |
PIRi | S64447 |
RefSeqi | NP_011654.1, NM_001181267.1 |
Genome annotation databases
EnsemblFungii | YGR138C_mRNA; YGR138C; YGR138C |
GeneIDi | 853039 |
KEGGi | sce:YGR138C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z72923 Genomic DNA Translation: CAA97151.1 BK006941 Genomic DNA Translation: DAA08230.1 |
PIRi | S64447 |
RefSeqi | NP_011654.1, NM_001181267.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 33385, 66 interactors |
DIPi | DIP-7902N |
IntActi | P53283, 26 interactors |
MINTi | P53283 |
STRINGi | 4932.YGR138C |
Protein family/group databases
TCDBi | 2.A.1.2.67, the major facilitator superfamily (mfs) |
PTM databases
iPTMneti | P53283 |
Proteomic databases
MaxQBi | P53283 |
PaxDbi | P53283 |
PRIDEi | P53283 |
Genome annotation databases
EnsemblFungii | YGR138C_mRNA; YGR138C; YGR138C |
GeneIDi | 853039 |
KEGGi | sce:YGR138C |
Organism-specific databases
SGDi | S000003370, TPO2 |
VEuPathDBi | FungiDB:YGR138C |
Phylogenomic databases
eggNOGi | KOG0255, Eukaryota |
GeneTreei | ENSGT00940000176486 |
HOGENOMi | CLU_008455_11_5_1 |
InParanoidi | P53283 |
OMAi | TWKEADP |
Miscellaneous databases
PROi | PR:P53283 |
RNActi | P53283, protein |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TPO2_YEAST | |
Accessioni | P53283Primary (citable) accession number: P53283 Secondary accession number(s): D6VUR9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | September 29, 2021 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - SIMILARITY comments
Index of protein domains and families