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Protein

Mitochondrial thiamine pyrophosphate carrier 1

Gene

TPC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.3 Publications

Kineticsi

  1. KM=0.51 mM for dATP uptake by unloaded proteoliposomes1 Publication
  1. Vmax=38 µmol/min/g enzyme with dATP as substrate uptaken by unloaded proteoliposomes1 Publication

GO - Molecular functioni

  • thiamine pyrophosphate transmembrane transporter activity Source: SGD

GO - Biological processi

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciYEAST:G3O-30806-MONOMER
ReactomeiR-SCE-196819 Vitamin B1 (thiamin) metabolism

Protein family/group databases

TCDBi2.A.29.28.1 the mitochondrial carrier (mc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial thiamine pyrophosphate carrier 1
Gene namesi
Name:TPC1
Ordered Locus Names:YGR096W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR096W
SGDiS000003328 TPC1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei14 – 30Helical; Name=1Sequence analysisAdd BLAST17
Transmembranei84 – 100Helical; Name=2Sequence analysisAdd BLAST17
Transmembranei116 – 136Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei170 – 186Helical; Name=4Sequence analysisAdd BLAST17
Transmembranei217 – 233Helical; Name=5Sequence analysisAdd BLAST17
Transmembranei285 – 302Helical; Name=6Sequence analysisAdd BLAST18

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000906961 – 314Mitochondrial thiamine pyrophosphate carrier 1Add BLAST314

Proteomic databases

PaxDbiP53257
PRIDEiP53257

Expressioni

Inductioni

Expression is regulated by carbon source, with very low expression with succinate, acetate, ethanol or pyruvate as carbon source.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi33339, 35 interactors
IntActiP53257, 1 interactor
MINTiP53257
STRINGi4932.YGR096W

Structurei

3D structure databases

ProteinModelPortaliP53257
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati14 – 103Solcar 1Add BLAST90
Repeati110 – 195Solcar 2Add BLAST86
Repeati210 – 310Solcar 3Add BLAST101

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074902
HOGENOMiHOG000165726
InParanoidiP53257
KOiK15108
OMAiWKGNVPA
OrthoDBiEOG092C3KDM

Family and domain databases

Gene3Di1.50.40.10, 1 hit
InterProiView protein in InterPro
IPR002067 Mit_carrier
IPR018108 Mitochondrial_sb/sol_carrier
IPR023395 Mt_carrier_dom_sf
PfamiView protein in Pfam
PF00153 Mito_carr, 3 hits
PRINTSiPR00926 MITOCARRIER
SUPFAMiSSF103506 SSF103506, 1 hit
PROSITEiView protein in PROSITE
PS50920 SOLCAR, 3 hits

Sequencei

Sequence statusi: Complete.

P53257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKEEDSLRK GQNVAAWKTL LAGAVSGLLA RSITAPMDTI KIRLQLTPAN
60 70 80 90 100
GLKPFGSQVM EVARSMIKNE GIRSFWKGNI PGSLLYVTYG SAQFSSYSLF
110 120 130 140 150
NRYLTPFGLE ARLHSLVVGA FAGITSSIVS YPFDVLRTRL VANNQMHSMS
160 170 180 190 200
ITREVRDIWK LEGLPGFFKG SIASMTTITL TASIMFGTYE TIRIYCDENE
210 220 230 240 250
KTTAAHKKWE LATLNHSAGT IGGVIAKIIT FPLETIRRRM QFMNSKHLEK
260 270 280 290 300
FSRHSSVYGS YKGYGFARIG LQILKQEGVS SLYRGILVAL SKTIPTTFVS
310
FWGYETAIHY LRMY
Length:314
Mass (Da):35,137
Last modified:October 1, 1996 - v1
Checksum:i1ED829A655ED03F4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66M → T in AAT93128 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72881 Genomic DNA Translation: CAA97099.1
AY693109 Genomic DNA Translation: AAT93128.1
BK006941 Genomic DNA Translation: DAA08189.1
PIRiS64401
RefSeqiNP_011610.3, NM_001181225.3

Genome annotation databases

EnsemblFungiiYGR096W; YGR096W; YGR096W
GeneIDi852988
KEGGisce:YGR096W

Similar proteinsi

Entry informationi

Entry nameiTPC1_YEAST
AccessioniPrimary (citable) accession number: P53257
Secondary accession number(s): D6VUM8, Q6B1H1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 18, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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