UniProtKB - P53220 (TIM21_YEAST)
Mitochondrial import inner membrane translocase subunit TIM21
TIM21
Functioni
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to keep the TOM and the TIM23 complexes in close contact. At some point, it is released from the TOM23 complex to allow protein translocation into the mitochondrial matrix. In the complex, it acts as an antagonist of TIM50 by reducing preprotein accumulation at the TOM23 complex and promotes dissociation of the PAM complex from the TIM23 complex.
2 PublicationsMiscellaneous
GO - Biological processi
- protein import into mitochondrial matrix Source: SGD
Keywordsi
Biological process | Protein transport, Translocation, Transport |
Protein family/group databases
TCDBi | 3.A.8.1.1, the mitochondrial protein translocase (mpt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Mitochondrial import inner membrane translocase subunit TIM21 |
Gene namesi | Name:TIM21 Ordered Locus Names:YGR033C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003265, TIM21 |
VEuPathDBi | FungiDB:YGR033C |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 2 Publications; Single-pass membrane protein 2 Publications
Mitochondrion
- mitochondrial inner membrane Source: Reactome
- mitochondrion Source: SGD
- TIM23 mitochondrial import inner membrane translocase complex Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 71 – 75 | Mitochondrial matrixSequence analysis | 5 | |
Transmembranei | 76 – 96 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 97 – 239 | Mitochondrial intermembraneSequence analysisAdd BLAST | 143 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 70 | MitochondrionSequence analysisAdd BLAST | 70 | |
ChainiPRO_0000043166 | 71 – 239 | Mitochondrial import inner membrane translocase subunit TIM21Add BLAST | 169 |
Proteomic databases
MaxQBi | P53220 |
PaxDbi | P53220 |
PRIDEi | P53220 |
Interactioni
Subunit structurei
Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21.
Interacts directly with TIM23.
Interacts with TOM22 component of the TOM complex. Released from the TOM23 complex by the PAM complex, leading to protein translocation into the mitochondrial matrix.
2 PublicationsBinary interactionsi
P53220
With | #Exp. | IntAct |
---|---|---|
TIM22 [Q12328] | 2 | EBI-23128,EBI-9132 |
TIM23 [P32897] | 16 | EBI-23128,EBI-9136 |
TIM50 [Q02776] | 10 | EBI-23128,EBI-30302 |
TOM22 [P49334] | 2 | EBI-23128,EBI-12527 |
Protein-protein interaction databases
BioGRIDi | 33278, 178 interactors |
ComplexPortali | CPX-6127, TIM23 mitochondrial inner membrane pre-sequence translocase complex, sort variant |
DIPi | DIP-3940N |
IntActi | P53220, 12 interactors |
MINTi | P53220 |
STRINGi | 4932.YGR033C |
Miscellaneous databases
RNActi | P53220, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P53220 |
SMRi | P53220 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P53220 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4836, Eukaryota |
HOGENOMi | CLU_089043_1_0_1 |
InParanoidi | P53220 |
OMAi | YEWGELS |
Family and domain databases
Gene3Di | 3.10.450.320, 1 hit |
InterProi | View protein in InterPro IPR013261, Tim21 IPR038552, Tim21_IMS_sf |
PANTHERi | PTHR13032, PTHR13032, 1 hit |
Pfami | View protein in Pfam PF08294, TIM21, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSSSLPRSLL RLGHRKPLFP RYNTFVNSSV ITHTSLLRTR LYSNGTGATS
60 70 80 90 100
GKKDDKTRNK PKPLWPQVKS ASTFTFSGIL VIGAVGISAI VIYLILSELF
110 120 130 140 150
SPSGDTQLFN RAVSMVEKNK DIRSLLQCDD GITGKERLKA YGELITNDKW
160 170 180 190 200
TRNRPIVSTK KLDKEGRTHH YMRFHVESKK KIALVHLEAK ESKQNYQPDF
210 220 230
INMYVDVPGE KRYYLIKPKL HPVSNSKGFL GIRWGPRKD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 39 | T → A in AAZ22448 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ115390 Genomic DNA Translation: AAZ22448.1 Z72818 Genomic DNA Translation: CAA97021.1 BK006941 Genomic DNA Translation: DAA08130.1 |
PIRi | S64324 |
RefSeqi | NP_011547.3, NM_001181162.3 |
Genome annotation databases
EnsemblFungii | YGR033C_mRNA; YGR033C; YGR033C |
GeneIDi | 852921 |
KEGGi | sce:YGR033C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ115390 Genomic DNA Translation: AAZ22448.1 Z72818 Genomic DNA Translation: CAA97021.1 BK006941 Genomic DNA Translation: DAA08130.1 |
PIRi | S64324 |
RefSeqi | NP_011547.3, NM_001181162.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CIU | X-ray | 1.60 | A | 103-225 | [»] | |
2MF7 | NMR | - | A | 103-225 | [»] | |
6K7D | X-ray | 2.00 | A | 105-217 | [»] | |
6K7E | X-ray | 1.53 | A | 105-217 | [»] | |
6K7F | X-ray | 1.80 | A | 105-217 | [»] | |
6K8Q | NMR | - | A | 105-217 | [»] | |
BMRBi | P53220 | |||||
SMRi | P53220 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33278, 178 interactors |
ComplexPortali | CPX-6127, TIM23 mitochondrial inner membrane pre-sequence translocase complex, sort variant |
DIPi | DIP-3940N |
IntActi | P53220, 12 interactors |
MINTi | P53220 |
STRINGi | 4932.YGR033C |
Protein family/group databases
TCDBi | 3.A.8.1.1, the mitochondrial protein translocase (mpt) family |
Proteomic databases
MaxQBi | P53220 |
PaxDbi | P53220 |
PRIDEi | P53220 |
Genome annotation databases
EnsemblFungii | YGR033C_mRNA; YGR033C; YGR033C |
GeneIDi | 852921 |
KEGGi | sce:YGR033C |
Organism-specific databases
SGDi | S000003265, TIM21 |
VEuPathDBi | FungiDB:YGR033C |
Phylogenomic databases
eggNOGi | KOG4836, Eukaryota |
HOGENOMi | CLU_089043_1_0_1 |
InParanoidi | P53220 |
OMAi | YEWGELS |
Miscellaneous databases
EvolutionaryTracei | P53220 |
PROi | PR:P53220 |
RNActi | P53220, protein |
Family and domain databases
Gene3Di | 3.10.450.320, 1 hit |
InterProi | View protein in InterPro IPR013261, Tim21 IPR038552, Tim21_IMS_sf |
PANTHERi | PTHR13032, PTHR13032, 1 hit |
Pfami | View protein in Pfam PF08294, TIM21, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TIM21_YEAST | |
Accessioni | P53220Primary (citable) accession number: P53220 Secondary accession number(s): D6VUG9, Q45U51 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | September 29, 2021 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families