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Protein

Mitochondrial import inner membrane translocase subunit TIM21

Gene

TIM21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to keep the TOM and the TIM23 complexes in close contact. At some point, it is released from the TOM23 complex to allow protein translocation into the mitochondrial matrix. In the complex, it acts as an antagonist of TIM50 by reducing preprotein accumulation at the TOM23 complex and promotes dissociation of the PAM complex from the TIM23 complex.2 Publications

Miscellaneous

Present with 3770 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • mitochondrial respiratory chain complex IV assembly Source: GO_Central
  • protein import into mitochondrial matrix Source: SGD

Keywordsi

Biological processProtein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30755-MONOMER

Protein family/group databases

TCDBi3.A.8.1.1 the mitochondrial protein translocase (mpt) family

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM21
Gene namesi
Name:TIM21
Ordered Locus Names:YGR033C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR033C
SGDiS000003265 TIM21

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini71 – 75Mitochondrial matrixSequence analysis5
Transmembranei76 – 96HelicalSequence analysisAdd BLAST21
Topological domaini97 – 239Mitochondrial intermembraneSequence analysisAdd BLAST143

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 70MitochondrionSequence analysisAdd BLAST70
ChainiPRO_000004316671 – 239Mitochondrial import inner membrane translocase subunit TIM21Add BLAST169

Proteomic databases

MaxQBiP53220
PaxDbiP53220
PRIDEiP53220

Interactioni

Subunit structurei

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with TOM22 component of the TOM complex. Released from the TOM23 complex by the PAM complex, leading to protein translocation into the mitochondrial matrix.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi33278, 176 interactors
ComplexPortaliCPX-539 Mitochondrial inner membrane pre-sequence translocase complex
DIPiDIP-3940N
IntActiP53220, 12 interactors
MINTiP53220
STRINGi4932.YGR033C

Structurei

Secondary structure

1239
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP53220
SMRiP53220
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53220

Family & Domainsi

Sequence similaritiesi

Belongs to the TIM21 family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000093236
InParanoidiP53220
KOiK17796
OMAiHFHVEGP
OrthoDBiEOG092C4QRS

Family and domain databases

Gene3Di3.10.450.320, 1 hit
InterProiView protein in InterPro
IPR013261 Tim21
IPR038552 Tim21_IMS_sf
PANTHERiPTHR13032 PTHR13032, 1 hit
PfamiView protein in Pfam
PF08294 TIM21, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53220-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSSLPRSLL RLGHRKPLFP RYNTFVNSSV ITHTSLLRTR LYSNGTGATS
60 70 80 90 100
GKKDDKTRNK PKPLWPQVKS ASTFTFSGIL VIGAVGISAI VIYLILSELF
110 120 130 140 150
SPSGDTQLFN RAVSMVEKNK DIRSLLQCDD GITGKERLKA YGELITNDKW
160 170 180 190 200
TRNRPIVSTK KLDKEGRTHH YMRFHVESKK KIALVHLEAK ESKQNYQPDF
210 220 230
INMYVDVPGE KRYYLIKPKL HPVSNSKGFL GIRWGPRKD
Length:239
Mass (Da):27,206
Last modified:October 1, 1996 - v1
Checksum:iFC140F3034E5B5BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39T → A in AAZ22448 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115390 Genomic DNA Translation: AAZ22448.1
Z72818 Genomic DNA Translation: CAA97021.1
BK006941 Genomic DNA Translation: DAA08130.1
PIRiS64324
RefSeqiNP_011547.3, NM_001181162.3

Genome annotation databases

EnsemblFungiiYGR033C; YGR033C; YGR033C
GeneIDi852921
KEGGisce:YGR033C

Similar proteinsi

Entry informationi

Entry nameiTIM21_YEAST
AccessioniPrimary (citable) accession number: P53220
Secondary accession number(s): D6VUG9, Q45U51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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