TIM21

Functionⁱ

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to keep the TOM and the TIM23 complexes in close contact. At some point, it is released from the TOM23 complex to allow protein translocation into the mitochondrial matrix. In the complex, it acts as an antagonist of TIM50 by reducing preprotein accumulation at the TOM23 complex and promotes dissociation of the PAM complex from the TIM23 complex.2 Publications

Miscellaneous

Present with 3770 molecules/cell in log phase SD medium.1 Publication

GO - Biological processⁱ

mitochondrial respiratory chain complex IV assembly Source: GO_Central
protein import into mitochondrial matrix
Source: SGD
Inferred from mutant phenotypeⁱ
- 15797382

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Protein transport, Translocation, Transport

Biological process

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-30755-MONOMER

BioCycⁱ

YEAST:G3O-30755-MONOMER

Protein family/group databases

TCDBⁱ	3.A.8.1.1 the mitochondrial protein translocase (mpt) family

TCDBⁱ

3.A.8.1.1 the mitochondrial protein translocase (mpt) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Mitochondrial import inner membrane translocase subunit TIM21
Gene namesⁱ	Name:TIM21 Ordered Locus Names:YGR033C
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome VII

Organism-specific databases

EuPathDBⁱ	FungiDB:YGR033C
Saccharomyces Genome Database More...SGDⁱ	S000003265 TIM21

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	71 – 75	Mitochondrial matrixSequence analysis	5
Transmembraneⁱ	76 – 96	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	97 – 239	Mitochondrial intermembraneSequence analysisAdd BLAST	143

Keywords - Cellular componentⁱ

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transit peptideⁱ	1 – 70	MitochondrionSequence analysisAdd BLAST		70
ChainⁱPRO_0000043166	71 – 239	Mitochondrial import inner membrane translocase subunit TIM21Add BLAST		169

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P53220
PaxDbⁱ	P53220
PRIDEⁱ	P53220

Interactionⁱ

Subunit structureⁱ

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with TOM22 component of the TOM complex. Released from the TOM23 complex by the PAM complex, leading to protein translocation into the mitochondrial matrix.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
TIM22	Q12328	2	EBI-23128,EBI-9132
TIM23	P32897	16	EBI-23128,EBI-9136
TIM50	Q02776	10	EBI-23128,EBI-30302
TOM22	P49334	2	EBI-23128,EBI-12527

Protein-protein interaction databases

BioGridⁱ	33278, 176 interactors
ComplexPortalⁱ	CPX-539 Mitochondrial inner membrane pre-sequence translocase complex
Database of interacting proteins More...DIPⁱ	DIP-3940N
IntActⁱ	P53220, 12 interactors
Molecular INTeraction database More...MINTⁱ	P53220
STRINGⁱ	4932.YGR033C

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	104 – 118	Combined sources	15
Helixⁱ	120 – 125	Combined sources	6
Beta strandⁱ	139 – 142	Combined sources	4
Beta strandⁱ	144 – 146	Combined sources	3
Beta strandⁱ	151 – 153	Combined sources	3
Beta strandⁱ	158 – 162	Combined sources	5
Beta strandⁱ	168 – 177	Combined sources	10
Beta strandⁱ	182 – 190	Combined sources	9
Beta strandⁱ	199 – 206	Combined sources	8
Beta strandⁱ	213 – 216	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	P53220
SMRⁱ	P53220
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P53220

EvolutionaryTraceⁱ

P53220

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the TIM21 family.Curated

Keywords - Domainⁱ

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMⁱ	HOG000093236
InParanoidⁱ	P53220
KEGG Orthology (KO) More...KOⁱ	K17796
OMAⁱ	HFHVEGP
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C4QRS

Family and domain databases

Gene3Dⁱ	3.10.450.320, 1 hit
InterProⁱ	View protein in InterPro IPR013261 Tim21 IPR038552 Tim21_IMS_sf
PANTHERⁱ	PTHR13032 PTHR13032, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08294 TIM21, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P53220-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSSSLPRSLL RLGHRKPLFP RYNTFVNSSV ITHTSLLRTR LYSNGTGATS 
        60         70         80         90        100
GKKDDKTRNK PKPLWPQVKS ASTFTFSGIL VIGAVGISAI VIYLILSELF 
       110        120        130        140        150
SPSGDTQLFN RAVSMVEKNK DIRSLLQCDD GITGKERLKA YGELITNDKW 
       160        170        180        190        200
TRNRPIVSTK KLDKEGRTHH YMRFHVESKK KIALVHLEAK ESKQNYQPDF 
       210        220        230 
INMYVDVPGE KRYYLIKPKL HPVSNSKGFL GIRWGPRKD

Length:239

Mass (Da):27,206

Last modified:October 1, 1996 - v1

Checksum:ⁱFC140F3034E5B5BB

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	39	T → A in AAZ22448 (Ref. 1) Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	DQ115390 Genomic DNA Translation: AAZ22448.1 Z72818 Genomic DNA Translation: CAA97021.1 BK006941 Genomic DNA Translation: DAA08130.1
PIRⁱ	S64324
NCBI Reference Sequences More...RefSeqⁱ	NP_011547.3, NM_001181162.3

Genome annotation databases

EnsemblFungiⁱ	YGR033C; YGR033C; YGR033C
GeneIDⁱ	852921
KEGGⁱ	sce:YGR033C

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P53220	Tim21p		YEASC		239	UniRef90_P53220
Tim21p		YEASL		250
Tim21p		YEASB		239
Uncharacterized protein		YEAS1		239
K7_Tim21p		YEASK		239
+9

Protein	Similar proteins		Species	Score	Length	Source
P53220	Tim21p		YEASC		239	UniRef50_P53220
Tim21p		YEASL		250
Tim21p		YEASB		239
Uncharacterized protein		YEAS1		239
K7_Tim21p		YEASK		239
+15

Entry informationⁱ

Entry nameⁱ	TIM21_YEAST
Accessionⁱ	P53220Primary (citable) accession number: P53220 Secondary accession number(s): D6VUG9, Q45U51
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
	Last sequence update:	October 1, 1996
	Last modified:	September 12, 2018
	This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

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Annotation systems

Supporting data

UniProtKB - P53220 (TIM21_YEAST)

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Mitochondrial import inner membrane translocase subunit TIM21

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Mitochondrion

Other locations

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi