UniProtKB - P53204 (NMA2_YEAST)
Protein
Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
Gene
NMA2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PubMed:12597897). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate to form deamido-NAD+ (NaAD). Key enzyme in both de novo and salvage pathways for NAD+ biosynthesis (By similarity). Predominantly acts in the salvage pathways via NMN (PubMed:11884393).By similarity2 Publications
Miscellaneous
Present with 1430 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Cofactori
Co2+1 PublicationNote: Divalent metal cation.1 Publication
Kineticsi
- KM=1.4 mM for ATP1 Publication
- KM=0.13 mM for NMN1 Publication
- KM=0.023 mM for NAD+1 Publication
- KM=5 mM for diphosphate1 Publication
pH dependencei
Optimum pH is 6.5-8.0.1 Publication
: NAD(+) biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes deamido-NAD(+) from nicotinate D-ribonucleotide.1 PublicationProteins known to be involved in this subpathway in this organism are:
- Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1 (NMA1), Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2 (NMA2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes deamido-NAD(+) from nicotinate D-ribonucleotide, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.
Pathwayi: NAD(+) biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from nicotinamide D-ribonucleotide.1 PublicationProteins known to be involved in this subpathway in this organism are:
- Nicotinamide mononucleotide adenylyltransferase (POF1), Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1 (NMA1), Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2 (NMA2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from nicotinamide D-ribonucleotide, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 175 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 166 – 168 | ATPBy similarity | 3 | |
Nucleotide bindingi | 282 – 284 | ATPBy similarity | 3 | |
Nucleotide bindingi | 350 – 353 | ATPBy similarity | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- nicotinamide-nucleotide adenylyltransferase activity Source: SGD
- nicotinate-nucleotide adenylyltransferase activity Source: GO_Central
GO - Biological processi
- NAD biosynthetic process Source: SGD
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Pyridine nucleotide biosynthesis |
Ligand | ATP-binding, NAD, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:YGR010W-MONOMER YEAST:YGR010W-MONOMER |
BRENDAi | 2.7.7.1 984 2.7.7.18 984 |
Reactomei | R-SCE-196807 Nicotinate metabolism |
UniPathwayi | UPA00253;UER00332 UPA00253;UER00600 |
Names & Taxonomyi
Protein namesi | Recommended name: Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2Curated (EC:2.7.7.11 Publication, EC:2.7.7.181 Publication)Short name: NMN/NaMN adenylyltransferase 2 Alternative name(s): NAD(+) diphosphorylase 2 NAD(+) pyrophosphorylase 2 Nicotinamide-nucleotide adenylyltransferase 21 Publication Short name: NMN adenylyltransferase 2 Short name: NMNAT 2 Nicotinate-nucleotide adenylyltransferase 21 Publication Short name: NaMN adenylyltransferase 2 Short name: NaMNAT 2 |
Gene namesi | Name:NMA21 Publication Ordered Locus Names:YGR010WImported |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YGR010W |
SGDi | S000003242 NMA2 |
Subcellular locationi
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000135019 | 1 – 395 | Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2Add BLAST | 395 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 85 | PhosphoserineCombined sources | 1 | |
Modified residuei | 89 | PhosphoserineCombined sources | 1 | |
Modified residuei | 90 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P53204 |
PaxDbi | P53204 |
PRIDEi | P53204 |
PTM databases
iPTMneti | P53204 |
Expressioni
Inductioni
Expression is slightly induced in late log phase.1 Publication
Interactioni
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
Q06178 | 6 | EBI-23073,EBI-11803 |
Protein-protein interaction databases
BioGridi | 33254, 48 interactors |
DIPi | DIP-1227N |
IntActi | P53204, 2 interactors |
MINTi | P53204 |
STRINGi | 4932.YGR010W |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 206 – 208 | Substrate bindingBy similarity | 3 | |
Regioni | 244 – 247 | Substrate bindingBy similarity | 4 | |
Regioni | 294 – 295 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the eukaryotic NMN adenylyltransferase family.Curated
Phylogenomic databases
HOGENOMi | HOG000216047 |
InParanoidi | P53204 |
KOi | K06210 |
OMAi | PAHHRVI |
Family and domain databases
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR004821 Cyt_trans-like IPR005248 NadD/NMNAT IPR014729 Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF01467 CTP_transf_like, 1 hit |
TIGRFAMsi | TIGR00482 TIGR00482, 1 hit |
i Sequence
Sequence statusi: Complete.
P53204-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDPTKAPDFK PPQPNEELQP PPDPTHTIPK SGPIVPYVLA DYNSSIDAPF
60 70 80 90 100
NLDIYKTLSS RKKNANSSNR MDHIPLNTSD FQPLSRDVSS EEESEGQSNG
110 120 130 140 150
IDATLQDVTM TGNLGVLKSQ IADLEEVPHT IVRQARTIED YEFPVHRLTK
160 170 180 190 200
KLQDPEKLPL IIVACGSFSP ITYLHLRMFE MALDDINEQT RFEVVGGYFS
210 220 230 240 250
PVSDNYQKRG LAPAYHRVRM CELACERTSS WLMVDAWESL QSSYTRTAKV
260 270 280 290 300
LDHFNHEINI KRGGIMTVDG EKMGVKIMLL AGGDLIESMG EPHVWADSDL
310 320 330 340 350
HHILGNYGCL IVERTGSDVR SFLLSHDIMY EHRRNILIIK QLIYNDISST
360 370 380 390
KVRLFIRRGM SVQYLLPNSV IRYIQEYNLY INQSEPVKQV LDSKE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z72795 Genomic DNA Translation: CAA96993.1 BK006941 Genomic DNA Translation: DAA08108.1 |
PIRi | S64299 |
RefSeqi | NP_011524.1, NM_001181139.1 |
Genome annotation databases
EnsemblFungii | YGR010W_mRNA; YGR010W; YGR010W |
GeneIDi | 852893 |
KEGGi | sce:YGR010W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z72795 Genomic DNA Translation: CAA96993.1 BK006941 Genomic DNA Translation: DAA08108.1 |
PIRi | S64299 |
RefSeqi | NP_011524.1, NM_001181139.1 |
3D structure databases
SMRi | P53204 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 33254, 48 interactors |
DIPi | DIP-1227N |
IntActi | P53204, 2 interactors |
MINTi | P53204 |
STRINGi | 4932.YGR010W |
PTM databases
iPTMneti | P53204 |
Proteomic databases
MaxQBi | P53204 |
PaxDbi | P53204 |
PRIDEi | P53204 |
Genome annotation databases
EnsemblFungii | YGR010W_mRNA; YGR010W; YGR010W |
GeneIDi | 852893 |
KEGGi | sce:YGR010W |
Organism-specific databases
EuPathDBi | FungiDB:YGR010W |
SGDi | S000003242 NMA2 |
Phylogenomic databases
HOGENOMi | HOG000216047 |
InParanoidi | P53204 |
KOi | K06210 |
OMAi | PAHHRVI |
Enzyme and pathway databases
UniPathwayi | UPA00253;UER00332 UPA00253;UER00600 |
BioCyci | MetaCyc:YGR010W-MONOMER YEAST:YGR010W-MONOMER |
BRENDAi | 2.7.7.1 984 2.7.7.18 984 |
Reactomei | R-SCE-196807 Nicotinate metabolism |
Miscellaneous databases
PROi | PR:P53204 |
Family and domain databases
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR004821 Cyt_trans-like IPR005248 NadD/NMNAT IPR014729 Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF01467 CTP_transf_like, 1 hit |
TIGRFAMsi | TIGR00482 TIGR00482, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NMA2_YEAST | |
Accessioni | P53204Primary (citable) accession number: P53204 Secondary accession number(s): D6VUE7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | October 16, 2019 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - SIMILARITY comments
Index of protein domains and families - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways