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Protein

APC/C activator protein CDH1

Gene

CDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). During telophase and in the subsequent G1 phase of the cell cycle, recognizes and binds proteins containing a destruction box (D-box) and an additional degradation signal termed the KEN box including ASE1, CDC20, the B-type cyclins CLB2 and CLB3, the polo-like kinase CDC5 and HSL1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for exit from mitosis, cytokinesis and formation of prereplicative complexes in G1. Probably is the target of a BUB2-dependent spindle checkpoint pathway.3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30527-MONOMER
ReactomeiR-SCE-141405 Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components
R-SCE-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-SCE-176408 Regulation of APC/C activators between G1/S and early anaphase
R-SCE-5689880 Ub-specific processing proteases
R-SCE-69656 Cyclin A:Cdk2-associated events at S phase entry

Names & Taxonomyi

Protein namesi
Recommended name:
APC/C activator protein CDH1
Alternative name(s):
CDC20 homolog 1
Homolog of CDC twenty 1
Gene namesi
Name:CDH1
Synonyms:HCT1
Ordered Locus Names:YGL003C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL003C
SGDiS000002971 CDH1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi12T → A: Abolishes phosphorylation; when associated with A-16; A-42; A-157; A-169; A-173; A-176; A-227 and A-239. 1 Publication1
Mutagenesisi16S → A: Abolishes phosphorylation; when associated with A-12; A-42; A-157; A-169; A-173; A-176; A-227 and A-239. 1 Publication1
Mutagenesisi42S → A: Abolishes phosphorylation; when associated with A-12; A-16; A-157; A-169; A-173; A-176; A-227 and A-239. 1 Publication1
Mutagenesisi157T → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-169; A-173; A-176; A-227 and A-239. 1 Publication1
Mutagenesisi169S → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-173; A-176; A-227 and A-239. 1 Publication1
Mutagenesisi173T → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-176; A-227 and A-239. 1 Publication1
Mutagenesisi176T → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-227 and A-239. 1 Publication1
Mutagenesisi227S → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-176 and A-239. 1 Publication1
Mutagenesisi239S → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-176 and A-227. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509041 – 566APC/C activator protein CDH1Add BLAST566

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei213PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at multiple sites by CDC28, probably in its CLB5 bound form, in S, G2 and M phase of the cell cycle, thereby blocking the association of CDH1 to the APC/C and promoting nuclear export of CDH1 by MSN5. Dephosphorylated and activated by CDC14 in late anaphase, which may be necessary for PSE1-dependent nuclear localization.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53197
PaxDbiP53197
PRIDEiP53197

PTM databases

iPTMnetiP53197

Interactioni

Subunit structurei

Associates with the APC/C complex. Interacts with CLB2, CLB3, CDC5, HSL1, MSN5 and PSE1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ACM1Q089818EBI-23684,EBI-2345174

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33242, 346 interactors
ComplexPortaliCPX-761 Anaphase-Promoting Complex variant 3
DIPiDIP-5915N
ELMiP53197
IntActiP53197, 22 interactors
MINTiP53197
STRINGi4932.YGL003C

Structurei

Secondary structure

1566
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP53197
SMRiP53197
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati258 – 298WD 1Add BLAST41
Repeati300 – 339WD 2Add BLAST40
Repeati342 – 379WD 3Add BLAST38
Repeati383 – 422WD 4Add BLAST40
Repeati425 – 467WD 5Add BLAST43
Repeati469 – 510WD 6Add BLAST42
Repeati513 – 552WD 7Add BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi55 – 61C-box7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi32 – 38Poly-Ser7

Domaini

The C-box is required for the association with the APC/C complex.By similarity

Sequence similaritiesi

Belongs to the WD repeat CDC20/Fizzy family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00870000136444
HOGENOMiHOG000195514
InParanoidiP53197
KOiK03364
OMAiQSRHGPN
OrthoDBiEOG092C2B79

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR024977 Apc4_WD40_dom
IPR033010 Cdc20/Fizzy
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19918 PTHR19918, 1 hit
PfamiView protein in Pfam
PF12894 ANAPC4_WD40, 1 hit
PF00400 WD40, 2 hits
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 3 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

P53197-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTNLNPFMN NTPSSSPLKG SESKRVSKRP ISSSSSASLL SSPSRRSRPS
60 70 80 90 100
TVYGDRYIPS RTDIDFNSIV SISSMASVPA LNPSSTEDQV EYQKERQAHE
110 120 130 140 150
TYNTLLKNEL FGEMLSKDTV GSESSIDRIK NTRPSTRGNV HAENTTRHGY
160 170 180 190 200
ELERVSTPPP EAAGLEEFSP HSTPVTPRRL FTSQQDEITR PSSNSVRGAS
210 220 230 240 250
LLTYQQRKGR RLSAASLLQS QFFDSMSPVR PDSKQLLLSP GKQFRQIAKV
260 270 280 290 300
PYRVLDAPSL ADDFYYSLID WSSTDVLAVA LGKSIFLTDN NTGDVVHLCD
310 320 330 340 350
TENEYTSLSW IGAGSHLAVG QANGLVEIYD VMKRKCIRTL SGHIDRVACL
360 370 380 390 400
SWNNHVLTSG SRDHRILHRD VRMPDPFFET IESHTQEVCG LKWNVADNKL
410 420 430 440 450
ASGGNDNVVH VYEGTSKSPI LTFDEHKAAV KAMAWSPHKR GVLATGGGTA
460 470 480 490 500
DRRLKIWNVN TSIKMSDIDS GSQICNMVWS KNTNELVTSH GYSKYNLTLW
510 520 530 540 550
DCNSMDPIAI LKGHSFRVLH LTLSNDGTTV VSGAGDETLR YWKLFDKPKA
560
KVQPNSLIFD AFNQIR
Length:566
Mass (Da):62,821
Last modified:October 1, 1996 - v1
Checksum:iDA6DE5545607906B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72525 Genomic DNA Translation: CAA96703.1
AY693078 Genomic DNA Translation: AAT93097.1
BK006941 Genomic DNA Translation: DAA08096.1
PIRiS64005
RefSeqiNP_011512.1, NM_001180868.1

Genome annotation databases

EnsemblFungiiYGL003C; YGL003C; YGL003C
GeneIDi852881
KEGGisce:YGL003C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72525 Genomic DNA Translation: CAA96703.1
AY693078 Genomic DNA Translation: AAT93097.1
BK006941 Genomic DNA Translation: DAA08096.1
PIRiS64005
RefSeqiNP_011512.1, NM_001180868.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BH6X-ray2.90A/B/C/D/E/F/G/H241-548[»]
5A31electron microscopy4.30R246-546[»]
ProteinModelPortaliP53197
SMRiP53197
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33242, 346 interactors
ComplexPortaliCPX-761 Anaphase-Promoting Complex variant 3
DIPiDIP-5915N
ELMiP53197
IntActiP53197, 22 interactors
MINTiP53197
STRINGi4932.YGL003C

PTM databases

iPTMnetiP53197

Proteomic databases

MaxQBiP53197
PaxDbiP53197
PRIDEiP53197

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL003C; YGL003C; YGL003C
GeneIDi852881
KEGGisce:YGL003C

Organism-specific databases

EuPathDBiFungiDB:YGL003C
SGDiS000002971 CDH1

Phylogenomic databases

GeneTreeiENSGT00870000136444
HOGENOMiHOG000195514
InParanoidiP53197
KOiK03364
OMAiQSRHGPN
OrthoDBiEOG092C2B79

Enzyme and pathway databases

BioCyciYEAST:G3O-30527-MONOMER
ReactomeiR-SCE-141405 Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components
R-SCE-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-SCE-176408 Regulation of APC/C activators between G1/S and early anaphase
R-SCE-5689880 Ub-specific processing proteases
R-SCE-69656 Cyclin A:Cdk2-associated events at S phase entry

Miscellaneous databases

PROiPR:P53197

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR024977 Apc4_WD40_dom
IPR033010 Cdc20/Fizzy
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19918 PTHR19918, 1 hit
PfamiView protein in Pfam
PF12894 ANAPC4_WD40, 1 hit
PF00400 WD40, 2 hits
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 3 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCDH1_YEAST
AccessioniPrimary (citable) accession number: P53197
Secondary accession number(s): D6VUD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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