UniProtKB - P53192 (GET1_YEAST)
Golgi to ER traffic protein 1
GET1
Functioni
Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET2, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in mitochondrial distribution and morphology.
UniRule annotation6 PublicationsMiscellaneous
GO - Molecular functioni
- protein-membrane adaptor activity Source: SGD
GO - Biological processi
- mitophagy Source: SGD
- protein insertion into ER membrane Source: ComplexPortal
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: SGD
- tail-anchored membrane protein insertion into ER membrane Source: InterPro
Keywordsi
Biological process | ER-Golgi transport, Transport |
Protein family/group databases
TCDBi | 3.A.21.1.1, the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family |
Names & Taxonomyi
Protein namesi | Recommended name: Golgi to ER traffic protein 1UniRule annotationAlternative name(s): Guided entry of tail-anchored proteins 1UniRule annotation Mitochondrial distribution and morphology protein 39 |
Gene namesi | Ordered Locus Names:YGL020C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002988, GET1 |
VEuPathDBi | FungiDB:YGL020C |
Subcellular locationi
Golgi apparatus
Endoplasmic reticulum
Mitochondrion
- Mitochondrion membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: Reactome
- GET complex Source: UniProtKB
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial membrane Source: UniProtKB-SubCell
- mitochondrion Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 | LumenalUniRule annotation | 1 | |
Transmembranei | 2 – 21 | HelicalUniRule annotationAdd BLAST | 20 | |
Topological domaini | 22 – 104 | CytoplasmicUniRule annotationAdd BLAST | 83 | |
Transmembranei | 105 – 125 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 126 – 181 | LumenalUniRule annotationAdd BLAST | 56 | |
Transmembranei | 182 – 198 | HelicalUniRule annotationAdd BLAST | 17 | |
Topological domaini | 199 – 235 | CytoplasmicUniRule annotationAdd BLAST | 37 |
Keywords - Cellular componenti
Endoplasmic reticulum, Golgi apparatus, Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000414847 | 1 – 235 | Golgi to ER traffic protein 1Add BLAST | 235 |
Proteomic databases
MaxQBi | P53192 |
PaxDbi | P53192 |
PRIDEi | P53192 |
Interactioni
Subunit structurei
Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Within the complex, GET1 and GET2 form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the GET3 homodimer (PubMed:32910895).
UniRule annotation5 PublicationsBinary interactionsi
P53192
With | #Exp. | IntAct |
---|---|---|
GET2 [P40056] | 3 | EBI-23722,EBI-22604 |
GET3 [Q12154] | 12 | EBI-23722,EBI-2989 |
Protein-protein interaction databases
BioGRIDi | 33226, 777 interactors |
ComplexPortali | CPX-956, GET complex |
DIPi | DIP-6309N |
IntActi | P53192, 3 interactors |
MINTi | P53192 |
STRINGi | 4932.YGL020C |
Miscellaneous databases
RNActi | P53192, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P53192 |
SMRi | P53192 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 68 – 104 | UniRule annotationAdd BLAST | 37 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4253, Eukaryota |
HOGENOMi | CLU_089418_2_1_1 |
InParanoidi | P53192 |
OMAi | AQDNYAR |
Family and domain databases
Gene3Di | 1.10.287.660, 1 hit |
HAMAPi | MF_03113, Get1, 1 hit |
InterProi | View protein in InterPro IPR028945, Get1 IPR027538, Get1_fungi IPR029012, Helix_hairpin_bin_sf |
Pfami | View protein in Pfam PF04420, CHD5, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MHWAAAVAIF FIVVTKFLQY TNKYHEKWIS KFAPGNELSK KYLAKVKERH
60 70 80 90 100
ELKEFNNSIS AQDNYAKWTK NNRKLDSLDK EINNLKDEIQ SENKAFQAHL
110 120 130 140 150
HKLRLLALTV PFFVFKIMYG KTPVYKLSSS TSTLFPTFVS GVWSQGWLYV
160 170 180 190 200
LLHPLRTISQ KWHIMEGKFG ASKFDDMALQ SVSLGIWVWA LMNVINGVEF
210 220 230
IVKQLFLTPK MEAPASVETQ EEKALDAVDD AIILD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z72542 Genomic DNA Translation: CAA96720.1 BK006941 Genomic DNA Translation: DAA08078.1 |
PIRi | S64022 |
RefSeqi | NP_011495.1, NM_001180885.1 |
Genome annotation databases
EnsemblFungii | YGL020C_mRNA; YGL020C; YGL020C |
GeneIDi | 852864 |
KEGGi | sce:YGL020C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z72542 Genomic DNA Translation: CAA96720.1 BK006941 Genomic DNA Translation: DAA08078.1 |
PIRi | S64022 |
RefSeqi | NP_011495.1, NM_001180885.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3B2E | X-ray | 3.00 | E/F/G/H | 21-104 | [»] | |
3SJA | X-ray | 3.00 | C/D/G/H/J | 36-93 | [»] | |
3SJB | X-ray | 3.30 | C/D | 19-103 | [»] | |
3SJC | X-ray | 3.20 | C/D/G/H | 36-93 | [»] | |
3VLC | X-ray | 4.50 | E | 21-104 | [»] | |
3ZS8 | X-ray | 3.00 | C/D | 21-104 | [»] | |
AlphaFoldDBi | P53192 | |||||
SMRi | P53192 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33226, 777 interactors |
ComplexPortali | CPX-956, GET complex |
DIPi | DIP-6309N |
IntActi | P53192, 3 interactors |
MINTi | P53192 |
STRINGi | 4932.YGL020C |
Protein family/group databases
TCDBi | 3.A.21.1.1, the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family |
Proteomic databases
MaxQBi | P53192 |
PaxDbi | P53192 |
PRIDEi | P53192 |
Genome annotation databases
EnsemblFungii | YGL020C_mRNA; YGL020C; YGL020C |
GeneIDi | 852864 |
KEGGi | sce:YGL020C |
Organism-specific databases
SGDi | S000002988, GET1 |
VEuPathDBi | FungiDB:YGL020C |
Phylogenomic databases
eggNOGi | KOG4253, Eukaryota |
HOGENOMi | CLU_089418_2_1_1 |
InParanoidi | P53192 |
OMAi | AQDNYAR |
Miscellaneous databases
PROi | PR:P53192 |
RNActi | P53192, protein |
Family and domain databases
Gene3Di | 1.10.287.660, 1 hit |
HAMAPi | MF_03113, Get1, 1 hit |
InterProi | View protein in InterPro IPR028945, Get1 IPR027538, Get1_fungi IPR029012, Helix_hairpin_bin_sf |
Pfami | View protein in Pfam PF04420, CHD5, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GET1_YEAST | |
Accessioni | P53192Primary (citable) accession number: P53192 Secondary accession number(s): D6VUB7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families