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Protein

Ubiquitin-conjugating enzyme variant MMS2

Gene

MMS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a role in the DNA error-free postreplication repair (PRR) pathway. Lacks catalytic activity by itself. The UBC13/MMS2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'.

Miscellaneous

Present with 2760 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • free ubiquitin chain polymerization Source: SGD
  • postreplication repair Source: SGD
  • protein K63-linked ubiquitination Source: GO_Central
  • protein polyubiquitination Source: SGD

Keywordsi

Molecular functionLigase
Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-30588-MONOMER
ReactomeiR-SCE-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-SCE-8866654 E3 ubiquitin ligases ubiquitinate target proteins
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme variant MMS2
Short name:
UEV MMS2
Gene namesi
Name:MMS2
Ordered Locus Names:YGL087C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL087C
SGDiS000003055 MMS2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi8F → A: Strongly reduces UBC13 binding and interferes with error-free DNA repair. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825981 – 137Ubiquitin-conjugating enzyme variant MMS2Add BLAST137

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53152
PaxDbiP53152
PRIDEiP53152

PTM databases

iPTMnetiP53152

Interactioni

Subunit structurei

Heterodimer with UBC13.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33163, 263 interactors
ComplexPortaliCPX-2541 MMS2-UBC13 ubiquitin ligase complex
DIPiDIP-5829N
IntActiP53152, 14 interactors
MINTiP53152
STRINGi4932.YGL087C

Structurei

Secondary structure

1137
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 18Combined sources13
Beta strandi26 – 32Combined sources7
Beta strandi40 – 46Combined sources7
Beta strandi49 – 51Combined sources3
Turni52 – 55Combined sources4
Beta strandi57 – 63Combined sources7
Turni66 – 70Combined sources5
Beta strandi74 – 79Combined sources6
Beta strandi84 – 86Combined sources3
Turni88 – 90Combined sources3
Helixi98 – 101Combined sources4
Helixi109 – 120Combined sources12
Helixi123 – 126Combined sources4

3D structure databases

ProteinModelPortaliP53152
SMRiP53152
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53152

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00740000115534
HOGENOMiHOG000036561
InParanoidiP53152
KOiK10704
OMAiGHTVHEN
OrthoDBiEOG092C4YVR

Family and domain databases

Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P53152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVPRNFRL LEELEKGEKG FGPESCSYGL ADSDDITMTK WNGTILGPPH
60 70 80 90 100
SNHENRIYSL SIDCGPNYPD SPPKVTFISK INLPCVNPTT GEVQTDFHTL
110 120 130
RDWKRAYTME TLLLDLRKEM ATPANKKLRQ PKEGETF
Length:137
Mass (Da):15,545
Last modified:October 1, 1996 - v1
Checksum:iE1DA713C94378795
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66724 Genomic DNA Translation: AAC24241.1
Z72609 Genomic DNA Translation: CAA96792.1
BK006941 Genomic DNA Translation: DAA08018.1
PIRiS64094
RefSeqiNP_011428.1, NM_001180952.1

Genome annotation databases

EnsemblFungiiYGL087C; YGL087C; YGL087C
GeneIDi852793
KEGGisce:YGL087C

Similar proteinsi

Entry informationi

Entry nameiMMS2_YEAST
AccessioniPrimary (citable) accession number: P53152
Secondary accession number(s): D6VU57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 18, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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