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UniProtKB - P53152 (MMS2_YEAST)
Protein
Ubiquitin-conjugating enzyme variant MMS2
Gene
MMS2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Has a role in the DNA error-free postreplication repair (PRR) pathway. Lacks catalytic activity by itself. The UBC13/MMS2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'.
Miscellaneous
Present with 2760 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- ligase activity Source: UniProtKB-KW
- ubiquitin-protein transferase activity Source: SGD
GO - Biological processi
- free ubiquitin chain polymerization Source: SGD
- postreplication repair Source: SGD
- protein K63-linked ubiquitination Source: GO_Central
- protein polyubiquitination Source: SGD
- protein targeting to vacuolar membrane Source: SGD
Keywordsi
Molecular function | Ligase |
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-SCE-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-SCE-9020702, Interleukin-1 signaling R-SCE-9646399, Aggrephagy R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-conjugating enzyme variant MMS2Short name: UEV MMS2 |
Gene namesi | Name:MMS2 Ordered Locus Names:YGL087C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003055, MMS2 |
VEuPathDBi | FungiDB:YGL087C |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleus Source: SGD
Vacuole
- fungal-type vacuole membrane Source: SGD
Other locations
- cytoplasm Source: SGD
- UBC13-MMS2 complex Source: ComplexPortal
- ubiquitin conjugating enzyme complex Source: SGD
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 8 | F → A: Strongly reduces UBC13 binding and interferes with error-free DNA repair. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082598 | 1 – 137 | Ubiquitin-conjugating enzyme variant MMS2Add BLAST | 137 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 71 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P53152 |
PaxDbi | P53152 |
PRIDEi | P53152 |
PTM databases
iPTMneti | P53152 |
Interactioni
Subunit structurei
Heterodimer with UBC13.
1 PublicationBinary interactionsi
P53152
With | #Exp. | IntAct |
---|---|---|
UBC13 [P52490] | 10 | EBI-11035,EBI-19777 |
Protein-protein interaction databases
BioGRIDi | 33163, 270 interactors |
ComplexPortali | CPX-2541, MMS2-UBC13 ubiquitin ligase complex |
DIPi | DIP-5829N |
IntActi | P53152, 10 interactors |
MINTi | P53152 |
STRINGi | 4932.YGL087C |
Miscellaneous databases
RNActi | P53152, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P53152 |
SMRi | P53152 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P53152 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 137 | UBC corePROSITE-ProRule annotationAdd BLAST | 133 |
Sequence similaritiesi
Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0896, Eukaryota |
HOGENOMi | CLU_063065_4_0_1 |
InParanoidi | P53152 |
OMAi | NLPCVDQ |
Family and domain databases
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS50127, UBC_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P53152-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKVPRNFRL LEELEKGEKG FGPESCSYGL ADSDDITMTK WNGTILGPPH
60 70 80 90 100
SNHENRIYSL SIDCGPNYPD SPPKVTFISK INLPCVNPTT GEVQTDFHTL
110 120 130
RDWKRAYTME TLLLDLRKEM ATPANKKLRQ PKEGETF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U66724 Genomic DNA Translation: AAC24241.1 Z72609 Genomic DNA Translation: CAA96792.1 BK006941 Genomic DNA Translation: DAA08018.1 |
PIRi | S64094 |
RefSeqi | NP_011428.1, NM_001180952.1 |
Genome annotation databases
EnsemblFungii | YGL087C_mRNA; YGL087C; YGL087C |
GeneIDi | 852793 |
KEGGi | sce:YGL087C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U66724 Genomic DNA Translation: AAC24241.1 Z72609 Genomic DNA Translation: CAA96792.1 BK006941 Genomic DNA Translation: DAA08018.1 |
PIRi | S64094 |
RefSeqi | NP_011428.1, NM_001180952.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JAT | X-ray | 1.60 | B | 1-137 | [»] | |
2GMI | X-ray | 2.50 | B | 1-137 | [»] | |
5OJW | X-ray | 2.00 | B | 1-137 | [»] | |
AlphaFoldDBi | P53152 | |||||
SMRi | P53152 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33163, 270 interactors |
ComplexPortali | CPX-2541, MMS2-UBC13 ubiquitin ligase complex |
DIPi | DIP-5829N |
IntActi | P53152, 10 interactors |
MINTi | P53152 |
STRINGi | 4932.YGL087C |
PTM databases
iPTMneti | P53152 |
Proteomic databases
MaxQBi | P53152 |
PaxDbi | P53152 |
PRIDEi | P53152 |
Genome annotation databases
EnsemblFungii | YGL087C_mRNA; YGL087C; YGL087C |
GeneIDi | 852793 |
KEGGi | sce:YGL087C |
Organism-specific databases
SGDi | S000003055, MMS2 |
VEuPathDBi | FungiDB:YGL087C |
Phylogenomic databases
eggNOGi | KOG0896, Eukaryota |
HOGENOMi | CLU_063065_4_0_1 |
InParanoidi | P53152 |
OMAi | NLPCVDQ |
Enzyme and pathway databases
Reactomei | R-SCE-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-SCE-9020702, Interleukin-1 signaling R-SCE-9646399, Aggrephagy R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
EvolutionaryTracei | P53152 |
PROi | PR:P53152 |
RNActi | P53152, protein |
Family and domain databases
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS50127, UBC_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MMS2_YEAST | |
Accessioni | P53152Primary (citable) accession number: P53152 Secondary accession number(s): D6VU57 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 181 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families