UniProtKB - P53131 (PRP43_YEAST)
Protein
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43
Gene
PRP43
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA.2 Publications
Miscellaneous
Present with 16900 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:3.6.4.13
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 116 – 123 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- mRNA binding Source: SGD
- RNA binding Source: GO_Central
- RNA helicase activity Source: SGD
GO - Biological processi
- maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
- maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
- maturation of SSU-rRNA Source: SGD
- maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
- ribosomal large subunit biogenesis Source: SGD
- rRNA processing Source: SGD
- spliceosomal complex disassembly Source: SGD
Keywordsi
Molecular function | Helicase, Hydrolase |
Biological process | mRNA processing, mRNA splicing |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43 (EC:3.6.4.13)Alternative name(s): Helicase JA1 |
Gene namesi | Name:PRP43 Ordered Locus Names:YGL120C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003088, PRP43 |
VEuPathDBi | FungiDB:YGL120C |
Subcellular locationi
Nucleus
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- post-mRNA release spliceosomal complex Source: SGD
- spliceosomal complex Source: GO_Central
Other locations
- 90S preribosome Source: SGD
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 194 | G → D in PRP43-DN1; dominant-negative phenotype. 1 Publication | 1 | |
Mutagenesisi | 247 | S → L in PRP43-DN2; dominant-negative phenotype. 1 Publication | 1 | |
Mutagenesisi | 395 | G → E in PRP43-1; temperature-sensitive. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055145 | 1 – 767 | Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43Add BLAST | 767 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 8 | PhosphoserineCombined sources | 1 | |
Modified residuei | 9 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P53131 |
PaxDbi | P53131 |
PRIDEi | P53131 |
PTM databases
iPTMneti | P53131 |
Interactioni
Subunit structurei
Component of the NTR complex (NTC-related complex), composed of NTR1, NTR2 and PRP43.
Interacts with NTR1 and NTR2.
Interacts with SPP382.
3 PublicationsBinary interactionsi
Hide detailsP53131
Protein-protein interaction databases
BioGRIDi | 33131, 467 interactors |
ComplexPortali | CPX-1604, Small ribosomal subunit processome, variant 1 CPX-1607, Small ribosomal subunit processome, variant 2 CPX-1608, Small ribosomal subunit processome, variant 3 CPX-1886, Post-mRNA release spliceosomal complex |
DIPi | DIP-5152N |
IntActi | P53131, 172 interactors |
MINTi | P53131 |
STRINGi | 4932.YGL120C |
Miscellaneous databases
RNActi | P53131, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P53131 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P53131 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 103 – 268 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 166 | |
Domaini | 293 – 473 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 181 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 215 – 218 | DEAH box | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0925, Eukaryota |
GeneTreei | ENSGT00940000175573 |
HOGENOMi | CLU_001832_5_11_1 |
InParanoidi | P53131 |
OMAi | QWCVDFA |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR002464, DNA/RNA_helicase_DEAH_CS IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
i Sequence
Sequence statusi: Complete.
P53131-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSKRRFSSE HPDPVETSIP EQAAEIAEEL SKQHPLPSEE PLVHHDAGEF
60 70 80 90 100
KGLQRHHTSA EEAQKLEDGK INPFTGREFT PKYVDILKIR RELPVHAQRD
110 120 130 140 150
EFLKLYQNNQ IMVFVGETGS GKTTQIPQFV LFDEMPHLEN TQVACTQPRR
160 170 180 190 200
VAAMSVAQRV AEEMDVKLGE EVGYSIRFEN KTSNKTILKY MTDGMLLREA
210 220 230 240 250
MEDHDLSRYS CIILDEAHER TLATDILMGL LKQVVKRRPD LKIIIMSATL
260 270 280 290 300
DAEKFQRYFN DAPLLAVPGR TYPVELYYTP EFQRDYLDSA IRTVLQIHAT
310 320 330 340 350
EEAGDILLFL TGEDEIEDAV RKISLEGDQL VREEGCGPLS VYPLYGSLPP
360 370 380 390 400
HQQQRIFEPA PESHNGRPGR KVVISTNIAE TSLTIDGIVY VVDPGFSKQK
410 420 430 440 450
VYNPRIRVES LLVSPISKAS AQQRAGRAGR TRPGKCFRLY TEEAFQKELI
460 470 480 490 500
EQSYPEILRS NLSSTVLELK KLGIDDLVHF DFMDPPAPET MMRALEELNY
510 520 530 540 550
LACLDDEGNL TPLGRLASQF PLDPMLAVML IGSFEFQCSQ EILTIVAMLS
560 570 580 590 600
VPNVFIRPTK DKKRADDAKN IFAHPDGDHI TLLNVYHAFK SDEAYEYGIH
610 620 630 640 650
KWCRDHYLNY RSLSAADNIR SQLERLMNRY NLELNTTDYE SPKYFDNIRK
660 670 680 690 700
ALASGFFMQV AKKRSGAKGY ITVKDNQDVL IHPSTVLGHD AEWVIYNEFV
710 720 730 740 750
LTSKNYIRTV TSVRPEWLIE IAPAYYDLSN FQKGDVKLSL ERIKEKVDRL
760
NELKQGKNKK KSKHSKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U41851 Genomic DNA Translation: AAB86458.1 Z72642 Genomic DNA Translation: CAA96828.1 BK006941 Genomic DNA Translation: DAA07989.1 |
PIRi | S64130 |
RefSeqi | NP_011395.1, NM_001180985.1 |
Genome annotation databases
EnsemblFungii | YGL120C_mRNA; YGL120C; YGL120C |
GeneIDi | 852757 |
KEGGi | sce:YGL120C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U41851 Genomic DNA Translation: AAB86458.1 Z72642 Genomic DNA Translation: CAA96828.1 BK006941 Genomic DNA Translation: DAA07989.1 |
PIRi | S64130 |
RefSeqi | NP_011395.1, NM_001180985.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2XAU | X-ray | 1.90 | A/B | 1-767 | [»] | |
3KX2 | X-ray | 2.20 | A/B | 1-767 | [»] | |
5I8Q | X-ray | 4.20 | A/B | 1-767 | [»] | |
5JPT | X-ray | 2.94 | A/B | 1-767 | [»] | |
5Y88 | electron microscopy | 3.70 | W | 1-767 | [»] | |
SMRi | P53131 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33131, 467 interactors |
ComplexPortali | CPX-1604, Small ribosomal subunit processome, variant 1 CPX-1607, Small ribosomal subunit processome, variant 2 CPX-1608, Small ribosomal subunit processome, variant 3 CPX-1886, Post-mRNA release spliceosomal complex |
DIPi | DIP-5152N |
IntActi | P53131, 172 interactors |
MINTi | P53131 |
STRINGi | 4932.YGL120C |
PTM databases
iPTMneti | P53131 |
Proteomic databases
MaxQBi | P53131 |
PaxDbi | P53131 |
PRIDEi | P53131 |
Genome annotation databases
EnsemblFungii | YGL120C_mRNA; YGL120C; YGL120C |
GeneIDi | 852757 |
KEGGi | sce:YGL120C |
Organism-specific databases
SGDi | S000003088, PRP43 |
VEuPathDBi | FungiDB:YGL120C |
Phylogenomic databases
eggNOGi | KOG0925, Eukaryota |
GeneTreei | ENSGT00940000175573 |
HOGENOMi | CLU_001832_5_11_1 |
InParanoidi | P53131 |
OMAi | QWCVDFA |
Miscellaneous databases
EvolutionaryTracei | P53131 |
PROi | PR:P53131 |
RNActi | P53131, protein |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR002464, DNA/RNA_helicase_DEAH_CS IPR011709, DUF1605 IPR007502, Helicase-assoc_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF04408, HA2, 1 hit PF00271, Helicase_C, 1 hit PF07717, OB_NTP_bind, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00847, HA2, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRP43_YEAST | |
Accessioni | P53131Primary (citable) accession number: P53131 Secondary accession number(s): D6VU28 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 198 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families