UniProtKB - P53128 (MTHR2_YEAST)
Protein
Methylenetetrahydrofolate reductase 2
Gene
MET13
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 8600 molecules/cell in log phase SD medium.1 Publication
Caution
Was originally thought to be a mitochondrial ribosomal protein.1 Publication
Catalytic activityi
- (6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP+ = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H+ + NADPHEC:1.5.1.20
- (6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD+ = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H+ + NADHEC:1.5.1.20
Cofactori
FADBy similarity
: tetrahydrofolate interconversion Pathwayi
This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 22 | Proton donor/acceptorBy similarity | 1 | |
Binding sitei | 84 | FADBy similarity | 1 | |
Binding sitei | 116 | SubstrateBy similarity | 1 | |
Binding sitei | 156 | FADBy similarity | 1 | |
Binding sitei | 171 | FADBy similarity | 1 | |
Binding sitei | 178 | FADBy similarity | 1 | |
Binding sitei | 189 | SubstrateBy similarity | 1 | |
Binding sitei | 282 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 22 – 27 | NADBy similarity | 6 | |
Nucleotide bindingi | 54 – 55 | NAD and FADBy similarity | 2 | |
Nucleotide bindingi | 114 – 116 | FADBy similarity | 3 | |
Nucleotide bindingi | 133 – 134 | FADBy similarity | 2 |
GO - Molecular functioni
- FAD binding Source: GO_Central
- methylenetetrahydrofolate reductase (NAD(P)H) activity Source: SGD
GO - Biological processi
- methionine biosynthetic process Source: SGD
- one-carbon metabolic process Source: GO_Central
- tetrahydrofolate interconversion Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein, NADP |
Enzyme and pathway databases
BRENDAi | 1.5.1.20, 984 |
Reactomei | R-SCE-196757, Metabolism of folate and pterines |
SABIO-RKi | P53128 |
UniPathwayi | UPA00193 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:MET13 Synonyms:MET11, MRPL45 Ordered Locus Names:YGL125W ORF Names:G2882 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003093, MET13 |
VEuPathDBi | FungiDB:YGL125W |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Mitochondrion
- mitochondrion Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000190256 | 1 – 600 | Methylenetetrahydrofolate reductase 2Add BLAST | 600 |
Proteomic databases
MaxQBi | P53128 |
PaxDbi | P53128 |
PRIDEi | P53128 |
PTM databases
CarbonylDBi | P53128 |
iPTMneti | P53128 |
Interactioni
Binary interactionsi
P53128
With | #Exp. | IntAct |
---|---|---|
MET12 [P46151] | 2 | EBI-11572,EBI-11567 |
Protein-protein interaction databases
BioGRIDi | 33126, 91 interactors |
DIPi | DIP-5188N |
IntActi | P53128, 3 interactors |
MINTi | P53128 |
STRINGi | 4932.YGL125W |
Miscellaneous databases
RNActi | P53128, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the methylenetetrahydrofolate reductase family.Curated
Phylogenomic databases
eggNOGi | KOG0564, Eukaryota |
HOGENOMi | CLU_025841_2_2_1 |
InParanoidi | P53128 |
OMAi | HLTCCNQ |
Family and domain databases
CDDi | cd00537, MTHFR, 1 hit |
InterProi | View protein in InterPro IPR029041, FAD-linked_oxidoreductase-like IPR004621, Fadh2_euk IPR003171, Mehydrof_redctse-like |
Pfami | View protein in Pfam PF02219, MTHFR, 1 hit |
SUPFAMi | SSF51730, SSF51730, 1 hit |
TIGRFAMsi | TIGR00677, fadh2_euk, 1 hit |
i Sequence
Sequence statusi: Complete.
P53128-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKITEKLEQH RQTSGKPTYS FEYFVPKTTQ GVQNLYDRMD RMYEASLPQF
60 70 80 90 100
IDITWNAGGG RLSHLSTDLV ATAQSVLGLE TCMHLTCTNM PISMIDDALE
110 120 130 140 150
NAYHSGCQNI LALRGDPPRD AENWTPVEGG FQYAKDLIKY IKSKYGDHFA
160 170 180 190 200
IGVAGYPECH PELPNKDVKL DLEYLKQKID AGGDFIITQM FYDVDNFINW
210 220 230 240 250
CSQVRAAGMD VPIIPGIMPI TTYAAFLRRA QWGQISIPQH FSSRLDPIKD
260 270 280 290 300
DDELVRDIGT NLIVEMCQKL LDSGYVSHLH IYTMNLEKAP LMILERLNIL
310 320 330 340 350
PTESEFNAHP LAVLPWRKSL NPKRKNEEVR PIFWKRRPYS YVARTSQWAV
360 370 380 390 400
DEFPNGRFGD SSSPAFGDLD LCGSDLIRQS ANKCLELWST PTSINDVAFL
410 420 430 440 450
VINYLNGNLK CLPWSDIPIN DEINPIKAHL IELNQHSIIT INSQPQVNGI
460 470 480 490 500
RSNDKIHGWG PKDGYVYQKQ YLEFMLPKTK LPKLIDTLKN NEFLTYFAID
510 520 530 540 550
SQGDLLSNHP DNSKSNAVTW GIFPGREILQ PTIVEKISFL AWKEEFYHIL
560 570 580 590 600
NEWKLNMNKY DKPHSAQFIQ SLIDDYCLVN IVDNDYISPD DQIHSILLSL
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 41 – 42 | RM → LA AA sequence (PubMed:9151978).Curated | 2 | |
Sequence conflicti | 48 | P → A AA sequence (PubMed:9151978).Curated | 1 | |
Sequence conflicti | 73 | A → R in CAA63833 (PubMed:8896269).Curated | 1 | |
Sequence conflicti | 152 – 154 | GVA → RC in AAC99805 (Ref. 5) Curated | 3 | |
Sequence conflicti | 183 | Missing in AAC99805 (Ref. 5) Curated | 1 | |
Sequence conflicti | 211 | V → L in AAC99805 (Ref. 5) Curated | 1 | |
Sequence conflicti | 233 – 243 | GQISIPQHFSS → ANLHPSTFLV in AAC99805 (Ref. 5) CuratedAdd BLAST | 11 | |
Sequence conflicti | 266 – 276 | MCQKLLDSGYV → CVKIARQWLR in AAC99805 (Ref. 5) CuratedAdd BLAST | 11 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z72647 Genomic DNA Translation: CAA96833.1 Sequence problems. X94106 Genomic DNA Translation: CAA63833.1 Sequence problems. U24271 Genomic DNA Translation: AAC99805.1 BK006941 Genomic DNA Translation: DAA07984.1 |
PIRi | S64136 |
RefSeqi | NP_011390.2, NM_001180990.1 |
Genome annotation databases
EnsemblFungii | YGL125W_mRNA; YGL125W; YGL125W |
GeneIDi | 852752 |
KEGGi | sce:YGL125W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z72647 Genomic DNA Translation: CAA96833.1 Sequence problems. X94106 Genomic DNA Translation: CAA63833.1 Sequence problems. U24271 Genomic DNA Translation: AAC99805.1 BK006941 Genomic DNA Translation: DAA07984.1 |
PIRi | S64136 |
RefSeqi | NP_011390.2, NM_001180990.1 |
3D structure databases
SMRi | P53128 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33126, 91 interactors |
DIPi | DIP-5188N |
IntActi | P53128, 3 interactors |
MINTi | P53128 |
STRINGi | 4932.YGL125W |
PTM databases
CarbonylDBi | P53128 |
iPTMneti | P53128 |
Proteomic databases
MaxQBi | P53128 |
PaxDbi | P53128 |
PRIDEi | P53128 |
Genome annotation databases
EnsemblFungii | YGL125W_mRNA; YGL125W; YGL125W |
GeneIDi | 852752 |
KEGGi | sce:YGL125W |
Organism-specific databases
SGDi | S000003093, MET13 |
VEuPathDBi | FungiDB:YGL125W |
Phylogenomic databases
eggNOGi | KOG0564, Eukaryota |
HOGENOMi | CLU_025841_2_2_1 |
InParanoidi | P53128 |
OMAi | HLTCCNQ |
Enzyme and pathway databases
UniPathwayi | UPA00193 |
BRENDAi | 1.5.1.20, 984 |
Reactomei | R-SCE-196757, Metabolism of folate and pterines |
SABIO-RKi | P53128 |
Miscellaneous databases
PROi | PR:P53128 |
RNActi | P53128, protein |
Family and domain databases
CDDi | cd00537, MTHFR, 1 hit |
InterProi | View protein in InterPro IPR029041, FAD-linked_oxidoreductase-like IPR004621, Fadh2_euk IPR003171, Mehydrof_redctse-like |
Pfami | View protein in Pfam PF02219, MTHFR, 1 hit |
SUPFAMi | SSF51730, SSF51730, 1 hit |
TIGRFAMsi | TIGR00677, fadh2_euk, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MTHR2_YEAST | |
Accessioni | P53128Primary (citable) accession number: P53128 Secondary accession number(s): D6VU23, O94090, Q92318 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 17, 2006 | |
Last modified: | February 10, 2021 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names