Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 137 (13 Feb 2019)
Sequence version 1 (01 Oct 1996)
Previous versions | rss
Other tutorials and videosHelp videoFeedback
Protein

Mediator of RNA polymerase II transcription subunit 5

Gene

NUT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.4 Publications

Miscellaneous

Present with 2491 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

  • regulation of transcription by RNA polymerase II Source: SGD
  • transcription by RNA polymerase II Source: SGD

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-30643-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 5
Alternative name(s):
Mediator complex subunit 5
Negative regulator of URS2 protein 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:NUT1
Synonyms:MED5
Ordered Locus Names:YGL151W
ORF Names:G1876
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome VII

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YGL151W

Saccharomyces Genome Database

More...
SGDi
S000003119 NUT1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000963871 – 1132Mediator of RNA polymerase II transcription subunit 5Add BLAST1132

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P53114

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P53114

PRoteomics IDEntifications database

More...
PRIDEi
P53114

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P53114

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins.1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
33102, 607 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-3226 Core mediator complex

Database of interacting proteins

More...
DIPi
DIP-6632N

Protein interaction database and analysis system

More...
IntActi
P53114, 32 interactors

Molecular INTeraction database

More...
MINTi
P53114

STRING: functional protein association networks

More...
STRINGi
4932.YGL151W

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
P53114

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P53114

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the Mediator complex subunit 5 family.Curated

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000113545

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P53114

KEGG Orthology (KO)

More...
KOi
K15147

Identification of Orthologs from Complete Genome Data

More...
OMAi
LHSSTYY

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR014801 Mediator_Med5_fun

The PANTHER Classification System

More...
PANTHERi
PTHR35784 PTHR35784, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF08689 Med5, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P53114-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEKESVYNLA LKCAERQLTS MEFSNLYKEF FNEKFPSLIQ EEEEDTTTTA
60 70 80 90 100
NINEVKKASD LVDTPSNNTA ATADTTHLHE ALDIVCSDFV KILNLEKPLI
110 120 130 140 150
LADYIVEVLL VNYNSDMIKC FLPKLNSVRN SLLLAHFFSK SCSFFAKLSD
160 170 180 190 200
TLIIDQVRKD LGNVIVPNIL SLDMNSMNKE LIAIVSKLLQ TTLKLSPSPI
210 220 230 240 250
LLTSAGCKNG SFTLLNQLSQ TNKLLFKRVS QTFEAKLHFK DTKPFLNKDS
260 270 280 290 300
TNEFVGSPSL TSPQYIPSPL SSTKPPGSVN SAAKYKDMKL LRYYKNIWLN
310 320 330 340 350
NKIINWEISN PDFLSKYSAI TSSIFQESFN SVQNLDQLLT DLIETSFTCF
360 370 380 390 400
AQFVSNKQYH QANSNLTLLE RKWVIFITKH LPLLILENSS RSPRVVTNAL
410 420 430 440 450
DNIDEKVVKA IRIYFTEKDD NKTNNEDLFD DYPSTSLDIR HDFIKGLIML
460 470 480 490 500
NLQPASVINN YLREDQMIDT SILPTRDDLF VRNLQGIQEV VHNTNSFIIS
510 520 530 540 550
SLDTLELESI TESITHDSSN GLFQVLHNFE SVAPTKQREI VKAFLSIFED
560 570 580 590 600
AIKELNYNRI AKICALLFFN FSHSLTTILS FSSPAALMKT LIKFVDLSRN
610 620 630 640 650
GRNGSNGNDE SSEYETINIS LSFSWAILLI INLTQTYGIS VVDVALKYPE
660 670 680 690 700
LSIKNSFIIN FISNLPNVSD KYYLEESNVN DSDMLTKSHN TVQSWLCDLF
710 720 730 740 750
VNGSITDQLI QNIETRQLAN LIPFIVKQVL LSVEIGVLTD ISSLIGGFEY
760 770 780 790 800
FLQPLLLVGL IKTFYWLEQF LSCVKNDTIS EDILQGIFNL LNTLFNPVTL
810 820 830 840 850
NEDSKAFHTA VLRLNAIPLL KVLRKFRVQS QSNYGIYSSD AQGDPNLEPL
860 870 880 890 900
IAKLVAVLNV SPVYDVDPRI INSENDYSRK QLGYGKFLIL NENPINKIMT
910 920 930 940 950
NQINSFWSLH SSTYYNLDYL FELIELVTPK SFLFDVLKTL EYKLATYGVP
960 970 980 990 1000
GSENKRGSLD SEHVFDYFFY FLVLYDVKTA EEASQLIEYM ENDAKKSKGD
1010 1020 1030 1040 1050
VDIKGEDLHE KNDSAEVRQE TQPKAEATQD DDFDMLFGEN DTSTQAYEEE
1060 1070 1080 1090 1100
EENEDNDGNN RTNNVPMIKA EETPSKTNKI SILKRHSFAV LLHERKLLND
1110 1120 1130
LALENGEITK TENEKFISYH DKYLCMLKTC VF
Length:1,132
Mass (Da):128,794
Last modified:October 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iBBD54661A4DB5F09
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Z48618 Genomic DNA No translation available.
Z72673 Genomic DNA Translation: CAA96863.1
BK006941 Genomic DNA Translation: DAA07960.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S60433

NCBI Reference Sequences

More...
RefSeqi
NP_011364.1, NM_001181016.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YGL151W_mRNA; YGL151W_mRNA; YGL151W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
852726

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YGL151W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48618 Genomic DNA No translation available.
Z72673 Genomic DNA Translation: CAA96863.1
BK006941 Genomic DNA Translation: DAA07960.1
PIRiS60433
RefSeqiNP_011364.1, NM_001181016.1

3D structure databases

ProteinModelPortaliP53114
SMRiP53114
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33102, 607 interactors
ComplexPortaliCPX-3226 Core mediator complex
DIPiDIP-6632N
IntActiP53114, 32 interactors
MINTiP53114
STRINGi4932.YGL151W

PTM databases

iPTMnetiP53114

Proteomic databases

MaxQBiP53114
PaxDbiP53114
PRIDEiP53114

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL151W_mRNA; YGL151W_mRNA; YGL151W
GeneIDi852726
KEGGisce:YGL151W

Organism-specific databases

EuPathDBiFungiDB:YGL151W
SGDiS000003119 NUT1

Phylogenomic databases

HOGENOMiHOG000113545
InParanoidiP53114
KOiK15147
OMAiLHSSTYY

Enzyme and pathway databases

BioCyciYEAST:G3O-30643-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P53114

Family and domain databases

InterProiView protein in InterPro
IPR014801 Mediator_Med5_fun
PANTHERiPTHR35784 PTHR35784, 1 hit
PfamiView protein in Pfam
PF08689 Med5, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMED5_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P53114
Secondary accession number(s): D6VTZ9
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 13, 2019
This is version 137 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again