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Protein

Oligo-1,6-glucosidase IMA1

Gene

IMA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major isomaltase (alpha-1,6-glucosidase) required for isomaltose utilization. Preferentially hydrolyzes isomaltose, palatinose, and methyl-alpha-glucoside, with little activity towards isomaltotriose or longer oligosaccharides. Does not hydrolyze maltose.2 Publications

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei215Nucleophile1
Active sitei277Proton donor1
Sitei352Transition state stabilizer1

GO - Molecular functioni

GO - Biological processi

  • disaccharide catabolic process Source: SGD
  • maltose metabolic process Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processMaltose metabolism

Enzyme and pathway databases

BioCyciYEAST:YGR287C-MONOMER
BRENDAi3.2.1.10 984

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13
mycoCLAPiOGL13C_YEAST

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase IMA1 (EC:3.2.1.10)
Alternative name(s):
Alpha-glucosidase
Isomaltase 1
Gene namesi
Name:IMA1
Ordered Locus Names:YGR287C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR287C
SGDiS000003519 IMA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi215D → A: Abolishes catalytic activity. 1 Publication1
Mutagenesisi216V → T: Can also hydrolyze maltose. 1 Publication1
Mutagenesisi217G → A: Does not alter substrate specificity. 1 Publication1
Mutagenesisi218S → G: Does not alter substrate specificity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5848

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000543332 – 589Oligo-1,6-glucosidase IMA1Add BLAST588

Proteomic databases

MaxQBiP53051
PaxDbiP53051
PRIDEiP53051

Expressioni

Inductioni

Expression is increased in response to the addition of maltose, isomaltose, and alpha-methylglucopyranoside.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399402EBI-10464,EBI-16219

Protein-protein interaction databases

BioGridi33537, 59 interactors
IntActiP53051, 5 interactors
STRINGi4932.YGR287C

Chemistry databases

BindingDBiP53051

Structurei

Secondary structure

1589
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP53051
SMRiP53051
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53051

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

GeneTreeiENSGT00900000140965
HOGENOMiHOG000220641
InParanoidiP53051
KOiK01182
OMAiRDWYWWR
OrthoDBiEOG092C12JX

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53051-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTISSAHPET EPKWWKEATF YQIYPASFKD SNDDGWGDMK GIASKLEYIK
60 70 80 90 100
ELGADAIWIS PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL
110 120 130 140 150
GMKFITDLVI NHCSSEHEWF KESRSSKTNP KRDWFFWRPP KGYDAEGKPI
160 170 180 190 200
PPNNWKSYFG GSAWTFDEKT QEFYLRLFCS TQPDLNWENE DCRKAIYESA
210 220 230 240 250
VGYWLDHGVD GFRIDVGSLY SKVVGLPDAP VVDKNSTWQS SDPYTLNGPR
260 270 280 290 300
IHEFHQEMNQ FIRNRVKDGR EIMTVGEMQH ASDETKRLYT SASRHELSEL
310 320 330 340 350
FNFSHTDVGT SPLFRYNLVP FELKDWKIAL AELFRYINGT DCWSTIYLEN
360 370 380 390 400
HDQPRSITRF GDDSPKNRVI SGKLLSVLLS ALTGTLYVYQ GQELGQINFK
410 420 430 440 450
NWPVEKYEDV EIRNNYNAIK EEHGENSEEM KKFLEAIALI SRDHARTPMQ
460 470 480 490 500
WSREEPNAGF SGPSAKPWFY LNDSFREGIN VEDEIKDPNS VLNFWKEALK
510 520 530 540 550
FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYNNKTLFAA LNFSSDATDF
560 570 580
KIPNDDSSFK LEFGNYPKKE VDASSRTLKP WEGRIYISE
Length:589
Mass (Da):68,592
Last modified:October 1, 1996 - v1
Checksum:i08869180588053B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti428E → D in BAD00094 (PubMed:15291818).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB109221 mRNA Translation: BAD00094.1
D43761 Genomic DNA Translation: BAA07818.1
Z73072 Genomic DNA Translation: CAA97319.1
Z73073 Genomic DNA Translation: CAA97321.1
BK006941 Genomic DNA Translation: DAA08375.1
PIRiS59370
RefSeqiNP_011803.3, NM_001181416.3

Genome annotation databases

EnsemblFungiiYGR287C; YGR287C; YGR287C
GeneIDi853204
KEGGisce:YGR287C

Similar proteinsi

Entry informationi

Entry nameiMALX3_YEAST
AccessioniPrimary (citable) accession number: P53051
Secondary accession number(s): D6VV64, Q76CZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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