ERG25

Functionⁱ

Catalyzes the first step in the removal of the two C-4 methyl groups of 4,4-dimethylzymosterol.2 Publications

Miscellaneous

Present with 77100 molecules/cell in log phase SD medium.1 Publication

Catalytic activityⁱ

4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + 6 ferrocytochrome b5 + 3 O₂ + 6 H⁺ = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + 6 ferricytochrome b5 + 4 H₂O.1 Publication

Cofactorⁱ

Fe cation1 Publication

Pathwayⁱ: zymosterol biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:

Lanosterol 14-alpha demethylase (ERG11)
Delta(14)-sterol reductase (ERG24)
Methylsterol monooxygenase (ERG25)
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (ERG26)
3-keto-steroid reductase (ERG27)
no protein annotated in this organism

This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

GO - Molecular functionⁱ

C-4 methylsterol oxidase activity
Source: SGD
Inferred from direct assayⁱ
- 8663358
iron ion binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

ergosterol biosynthetic process
Source: SGD
Inferred from direct assayⁱ
- 8663358

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Biological process	Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
Ligand	Iron, NAD

Enzyme and pathway databases

BioCycⁱ	MetaCyc:YGR060W-MONOMER YEAST:YGR060W-MONOMER
Reactomeⁱ	R-SCE-191273 Cholesterol biosynthesis R-SCE-192105 Synthesis of bile acids and bile salts
UniPathwayⁱ	UPA00770;UER00756

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Methylsterol monooxygenase (EC:1.14.18.91 Publication) Alternative name(s): C-4 methylsterol oxidase
Gene namesⁱ	Name:ERG25 Synonyms:FET6 Ordered Locus Names:YGR060W
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome VII

Organism-specific databases

EuPathDBⁱ	FungiDB:YGR060W
Saccharomyces Genome Database More...SGDⁱ	S000003292 ERG25

Topology

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transmembraneⁱ	56 – 76	HelicalSequence analysisAdd BLAST		21

Keywords - Cellular componentⁱ

Endoplasmic reticulum, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000117039	1 – 309	Methylsterol monooxygenaseAdd BLAST		309

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Cross-linkⁱ	96	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMⁱ

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P53045
PaxDbⁱ	P53045
PRIDEⁱ	P53045

PTM databases

iPTMnetⁱ	P53045

iPTMnetⁱ

P53045

Interactionⁱ

Subunit structureⁱ

Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum. Interacts with ERG27 and ERG28.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
ERG11	P10614	3	EBI-6506,EBI-5127
ERG27	Q12452	4	EBI-6506,EBI-38132
ERG28	P40030	3	EBI-6506,EBI-22518
ERG3	P32353	3	EBI-6506,EBI-6554

Protein-protein interaction databases

BioGridⁱ	33305, 312 interactors
Database of interacting proteins More...DIPⁱ	DIP-2799N
IntActⁱ	P53045, 60 interactors
Molecular INTeraction database More...MINTⁱ	P53045
STRINGⁱ	4932.YGR060W

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P53045
SMRⁱ	P53045
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Length
Motifⁱ	160 – 164	Histidine box-1	5
Motifⁱ	173 – 177	Histidine box-2	5
Motifⁱ	257 – 263	Histidine box-3	7

Domainⁱ

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similaritiesⁱ

Belongs to the sterol desaturase family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063017
HOGENOMⁱ	HOG000162289
InParanoidⁱ	P53045
KEGG Orthology (KO) More...KOⁱ	K07750
OMAⁱ	QFNCNFA
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C3Y3T

Family and domain databases

InterProⁱ	View protein in InterPro IPR006694 Fatty_acid_hydroxylase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04116 FA_hydroxylase, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P53045-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSAVFNNATL SGLVQASTYS QTLQNVAHYQ PQLNFMEKYW AAWYSYMNND 
        60         70         80         90        100
VLATGLMFFL LHEFMYFFRC LPWFIIDQIP YFRRWKLQPT KIPSAKEQLY 
       110        120        130        140        150
CLKSVLLSHF LVEAIPIWTF HPMCEKLGIT VEVPFPSLKT MALEIGLFFV 
       160        170        180        190        200
LEDTWHYWAH RLFHYGVFYK YIHKQHHRYA APFGLSAEYA HPAETLSLGF 
       210        220        230        240        250
GTVGMPILYV MYTGKLHLFT LCVWITLRLF QAVDSHSGYD FPWSLNKIMP 
       260        270        280        290        300
FWAGAEHHDL HHHYFIGNYA SSFRWWDYCL DTESGPEAKA SREERMKKRA 

ENNAQKKTN

Length:309

Mass (Da):36,479

Last modified:October 1, 1996 - v1

Checksum:ⁱE0422C416DD17794

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	302	N → K in AAS56153 (PubMed:17322287).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U31885 Genomic DNA Translation: AAC49139.1 DQ115391 Genomic DNA Translation: AAZ22473.1 Z72845 Genomic DNA Translation: CAA97062.1 AY557827 Genomic DNA Translation: AAS56153.1 BK006941 Genomic DNA Translation: DAA08156.1
PIRⁱ	S64354
NCBI Reference Sequences More...RefSeqⁱ	NP_011574.3, NM_001181189.3

Genome annotation databases

EnsemblFungiⁱ	YGR060W; YGR060W; YGR060W
GeneIDⁱ	852951
KEGGⁱ	sce:YGR060W

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P53045	C-4 sterol methyl oxidase		YEAS1		309	UniRef100_P53045
K7_Erg25p		YEASK		309
Erg25p		YEASA		309
Erg25p		SACCK		309
ERG25p C-4 methyl sterol oxidase		Saccharomyces sp. 'boulardii'		309
+5

Protein	Similar proteins		Species	Score	Length	Source
P53045	C-4 sterol methyl oxidase		YEAS1		309	UniRef90_P53045
K7_Erg25p		YEASK		309
Erg25p		YEASA		309
Erg25p		SACCK		309
ERG25p C-4 methyl sterol oxidase		Saccharomyces sp. 'boulardii'		309
+14

Protein	Similar proteins		Species	Score	Length	Source
P53045	C-4 sterol methyl oxidase		YEAS1		309	UniRef50_P53045
K7_Erg25p		YEASK		309
Erg25p		YEASA		309
Erg25p		SACCK		309
ERG25p C-4 methyl sterol oxidase		Saccharomyces sp. 'boulardii'		309
+108

Entry informationⁱ

Entry nameⁱ	MSMO_YEAST
Accessionⁱ	P53045Primary (citable) accession number: P53045 Secondary accession number(s): D6VUJ5, E9P8T3, Q45U26
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
	Last sequence update:	October 1, 1996
	Last modified:	September 12, 2018
	This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P53045 (MSMO_YEAST)

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Methylsterol monooxygenase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: zymosterol biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

Endoplasmic reticulum

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi