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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Kitasatospora aureofaciens (Streptomyces aureofaciens)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei440NucleophileBy similarity1
Active sitei493Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
OrganismiKitasatospora aureofaciens (Streptomyces aureofaciens)
Taxonomic identifieri1894 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeKitasatospora

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001887461 – 7641,4-alpha-glucan branching enzyme GlgBAdd BLAST764

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP52980
SMRiP52980
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
HAMAPiMF_00685 GlgB, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR037439 Branching_enzy
IPR006407 GlgB
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PANTHERiPTHR43651 PTHR43651, 1 hit
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF02922 CBM_48, 1 hit
PIRSFiPIRSF000463 GlgB, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
TIGRFAMsiTIGR01515 branching_enzym, 1 hit

Sequencei

Sequence statusi: Complete.

P52980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAARQPSPT VRDKAAPEPA APAAPKGARA PRARRAAPPH GVRPAPALAA
60 70 80 90 100
EERARLLEGR HHDPHAVLGA RTQRGGVAFR VLRPYAKAVT VVAKGLRTEL
110 120 130 140 150
VDEGDGLFSG LLPLTGVPDY RLLVTYDSDE IEVHDPYRFL PALGELDLHL
160 170 180 190 200
IGEGRHEELW TALGSQPMEH QGVAGTRFTV WAPNALGVRV TGDFSYWDAV
210 220 230 240 250
AYPMRSLGAS GVWELFLPGV AEGALYKYEI TRPDGGRTLR ADPMARYAEV
260 270 280 290 300
PPANASIVTA SRYEWQDAEW MARRGALAPH QAPMSVYELH LASWRPGLSY
310 320 330 340 350
RQLAEQLPAY VKELGFTHVE LMPVAEHPFG GSWGYQVTGF YAPTSRMGTP
360 370 380 390 400
DDFRFLVDAL HRAGIGVIVD WVPAHFPRDD WALAEFDGRP LYEHQDPRRA
410 420 430 440 450
AHPDWGTLEF DYGRKEVRNF LVANAVYWCQ EFHVDGLRAD AVASMLYLDY
460 470 480 490 500
SRDEGDWSPN AHGGREDLDA VALLQEMNAT VYRRFPGVVT IAEESTAWDG
510 520 530 540 550
VTRPTDSGGL GFGLKWNMGW MHDTLRYVSK EPVHRKYHHH DMTFGMVYAF
560 570 580 590 600
SENFVLPISH DEVVHGKRSL VSKMPGDWWQ QRATHRAYLG FMWAHPGKQL
610 620 630 640 650
LFMGQEFAQG SEWSETYGPD WWVLDSSYPA AGDHLGVRSL VRDLNRTYTA
660 670 680 690 700
SPALWERDSV PEGFAWVEAD AADDNVFAFL RFARDGSPLL CVSNFSPVVR
710 720 730 740 750
HGYRIGVPQE VGQWREVLNT DLEPYGGSGV HHARALRPEP VPAQGRAVSL
760
RMTLPPMATV WLRP
Length:764
Mass (Da):85,325
Last modified:October 1, 1996 - v1
Checksum:i6B45482E4A268ACF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11647 Genomic DNA Translation: AAA67437.1
PIRiS47569

Similar proteinsi

Entry informationi

Entry nameiGLGB_KITAU
AccessioniPrimary (citable) accession number: P52980
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 18, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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