UniProtKB - P52912 (TIA1_MOUSE)
Cytotoxic granule associated RNA binding protein TIA1
Tia1
Functioni
RNA-binding protein involved in the regulation of alternative pre-RNA splicing and mRNA translation by binding to uridine-rich (U-rich) RNA sequences (PubMed:10938105, PubMed:16227602).
Binds to U-rich sequences immediately downstream from a 5' splice sites in a uridine-rich small nuclear ribonucleoprotein (U snRNP)-dependent fashion, thereby modulating alternative pre-RNA splicing (PubMed:10938105).
Preferably binds to the U-rich IAS1 sequence in a U1 snRNP-dependent manner; this binding is optimal if a 5' splice site is adjacent to IAS1 (PubMed:10938105).
Activates the use of heterologous 5' splice sites; the activation depends on the intron sequence downstream from the 5' splice site, with a preference for a downstream U-rich sequence (PubMed:10938105).
By interacting with SNRPC/U1-C, promotes recruitment and binding of spliceosomal U1 snRNP to 5' splice sites followed by U-rich sequences, thereby facilitating atypical 5' splice site recognition by U1 snRNP (By similarity).
Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIAR mRNA (PubMed:11514562).
Acts as a modulator of alternative splicing for the apoptotic FAS receptor, thereby promoting apoptosis (By similarity).
Binds to the 5' splice site region of FAS intron 5 to promote accumulation of transcripts that include exon 6 at the expense of transcripts in which exon 6 is skipped, thereby leading to the transcription of a membrane-bound apoptotic FAS receptor, which promotes apoptosis (By similarity).
Binds to a conserved AU-rich cis element in COL2A1 intron 2 and modulates alternative splicing of COL2A1 exon 2 (By similarity).
Also binds to the equivalent AT-rich element in COL2A1 genomic DNA, and may thereby be involved in the regulation of transcription (By similarity).
Involved in the repression of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs), including target ARE-bearing mRNAs encoding TNF and PTGS2 (PubMed:16227602, PubMed:10921895).
Also participates in the cellular response to environmental stress, by acting downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SGs), leading to stress-induced translational arrest (By similarity).
Formation and recruitment to SGs is regulated by Zn2+ (PubMed:29298433).
Possesses nucleolytic activity against cytotoxic lymphocyte target cells (By similarity).
By similarity5 PublicationsGO - Molecular functioni
- mRNA 3'-UTR AU-rich region binding Source: MGI
- mRNA 3'-UTR binding Source: UniProtKB
- RNA binding Source: MGI
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- negative regulation of cytokine production Source: MGI
- negative regulation of translation Source: UniProtKB
- positive regulation of epithelial cell apoptotic process Source: MGI
- protein localization to cytoplasmic stress granule Source: MGI
- regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
- regulation of mRNA splicing, via spliceosome Source: MGI
- stress granule assembly Source: UniProtKB
Keywordsi
Molecular function | RNA-binding |
Biological process | Apoptosis, mRNA processing, mRNA splicing |
Enzyme and pathway databases
Reactomei | R-MMU-6803529, FGFR2 alternative splicing |
Names & Taxonomyi
Protein namesi | Recommended name: Cytotoxic granule associated RNA binding protein TIA1ImportedAlternative name(s): Nucleolysin TIA-1Curated RNA-binding protein TIA-1 T-cell-restricted intracellular antigen-1 Short name: TIA-1 |
Gene namesi | Name:Tia1 Synonyms:Tia |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107914, Tia1 |
VEuPathDBi | HostDB:ENSMUSG00000071337 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- Stress granule 1 Publication
Note: Accumulates in cytoplasmic stress granules (SG) following cellular damage (PubMed:29298433). Recruitment to SG is induced by Zn2+ (PubMed:29298433).1 Publication
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear stress granule Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- cytoplasmic stress granule Source: UniProtKB
- ribonucleoprotein complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081977 | 1 – 386 | Cytotoxic granule associated RNA binding protein TIA1Add BLAST | 386 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
AcetylationProteomic databases
EPDi | P52912 |
PaxDbi | P52912 |
PRIDEi | P52912 |
ProteomicsDBi | 262777 |
PTM databases
iPTMneti | P52912 |
PhosphoSitePlusi | P52912 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000071337, Expressed in midbrain and 305 other tissues |
ExpressionAtlasi | P52912, baseline and differential |
Genevisiblei | P52912, MM |
Interactioni
Subunit structurei
Homooligomer; homooligomerization is induced by Zn2+ (PubMed:29298433).
Interacts with FASTK; the interactions leads to its phosphorylation (By similarity).
Interacts (via RRM1 and the C-terminal glutamine-rich (Q) sequence) with SNRPC/U1-C (via N-terminus); thereby facilitating spliceosomal U1 snRNP recruitment to 5' splice sites (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 204190, 189 interactors |
CORUMi | P52912 |
IntActi | P52912, 1 interactor |
MINTi | P52912 |
STRINGi | 10090.ENSMUSP00000093425 |
Miscellaneous databases
RNActi | P52912, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P52912 |
SMRi | P52912 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P52912 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 83 | RRM 1PROSITE-ProRule annotationAdd BLAST | 77 | |
Domaini | 106 – 184 | RRM 2PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 214 – 286 | RRM 3PROSITE-ProRule annotationAdd BLAST | 73 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 355 – 376 | DisorderedSequence analysisAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 355 – 373 | Polar residuesSequence analysisAdd BLAST | 19 |
Domaini
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0148, Eukaryota |
GeneTreei | ENSGT00940000154962 |
InParanoidi | P52912 |
OMAi | ESFQNQH |
OrthoDBi | 1066369at2759 |
PhylomeDBi | P52912 |
TreeFami | TF312915 |
Family and domain databases
CDDi | cd12615, RRM1_TIA1, 1 hit cd12618, RRM2_TIA1, 1 hit cd12621, RRM3_TIA1, 1 hit |
Gene3Di | 3.30.70.330, 3 hits |
IDEALi | IID50284 |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR003954, RRM_dom_euk IPR034827, TIA-1_RRM1 IPR034830, TIA1_RRM2 IPR034832, TIA1_RRM3 |
Pfami | View protein in Pfam PF00076, RRM_1, 3 hits |
SMARTi | View protein in SMART SM00360, RRM, 3 hits SM00361, RRM_1, 3 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MEDEMPKTLY VGNLSRDVTE ALILQLFSQI GPCKNCKMIM DTAGNDPYCF
60 70 80 90 100
VEFHEHRHAA AALAAMNGRK IMGKEVKVNW ATTPSSQKKD TSSSTVVSTQ
110 120 130 140 150
RSQDHFHVFV GDLSPEITTE DIKAAFAPFG RISDARVVKD MATGKSKGYG
160 170 180 190 200
FVSFFNKWDA ENAIQQMGGQ WLGGRQIRTN WATRKPPAPK STYESNTKQL
210 220 230 240 250
SYDEVVSQSS PNNCTVYCGG VTSGLTEQLM RQTFSPFGQI MEIRVFPDKG
260 270 280 290 300
YSFVRFSSHE SAAHAIVSVN GTTIEGHVVK CYWGKETLDM INPVQQQNQI
310 320 330 340 350
GYPPTYGQWG QWYGNAQQIG QYVPNGWQVP AYGVYGQPWS QQGFNQTQSS
360 370 380
APWMGPNYSV PPPQGQNGSM LPSQPAGYRV AGYETQ
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ80ZW7 | Q80ZW7_MOUSE | Cytotoxic granule-associated RNA-bi... | Tia1 | 377 | Annotation score: | ||
G5E8L2 | G5E8L2_MOUSE | Cytotoxic granule-associated RNA-bi... | Tia1 | 375 | Annotation score: | ||
Q8CII5 | Q8CII5_MOUSE | Cytotoxic granule-associated RNA-bi... | Tia1 | 285 | Annotation score: | ||
D3Z4H6 | D3Z4H6_MOUSE | Cytotoxic granule-associated RNA-bi... | Tia1 | 385 | Annotation score: | ||
D3YY02 | D3YY02_MOUSE | Cytotoxic granule-associated RNA-bi... | Tia1 | 212 | Annotation score: | ||
A0A0N4SUX1 | A0A0N4SUX1_MOUSE | Cytotoxic granule-associated RNA-bi... | Tia1 | 133 | Annotation score: | ||
A0A0N4SVR8 | A0A0N4SVR8_MOUSE | Cytotoxic granule-associated RNA-bi... | Tia1 | 93 | Annotation score: | ||
A0A0N4SW78 | A0A0N4SW78_MOUSE | Cytotoxic granule-associated RNA-bi... | Tia1 | 105 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00689 mRNA Translation: AAA03711.1 U55862 mRNA Translation: AAC52871.1 |
CCDSi | CCDS20312.1 |
PIRi | S72435 |
RefSeqi | NP_035715.1, NM_011585.4 |
Genome annotation databases
Ensembli | ENSMUST00000095753; ENSMUSP00000093425; ENSMUSG00000071337 |
GeneIDi | 21841 |
KEGGi | mmu:21841 |
UCSCi | uc009crp.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00689 mRNA Translation: AAA03711.1 U55862 mRNA Translation: AAC52871.1 |
CCDSi | CCDS20312.1 |
PIRi | S72435 |
RefSeqi | NP_035715.1, NM_011585.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DGO | NMR | - | A | 87-199 | [»] | |
2RNE | NMR | - | A | 87-199 | [»] | |
AlphaFoldDBi | P52912 | |||||
SMRi | P52912 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204190, 189 interactors |
CORUMi | P52912 |
IntActi | P52912, 1 interactor |
MINTi | P52912 |
STRINGi | 10090.ENSMUSP00000093425 |
PTM databases
iPTMneti | P52912 |
PhosphoSitePlusi | P52912 |
Proteomic databases
EPDi | P52912 |
PaxDbi | P52912 |
PRIDEi | P52912 |
ProteomicsDBi | 262777 |
Protocols and materials databases
Antibodypediai | 16302, 539 antibodies from 43 providers |
DNASUi | 21841 |
Genome annotation databases
Ensembli | ENSMUST00000095753; ENSMUSP00000093425; ENSMUSG00000071337 |
GeneIDi | 21841 |
KEGGi | mmu:21841 |
UCSCi | uc009crp.2, mouse |
Organism-specific databases
CTDi | 7072 |
MGIi | MGI:107914, Tia1 |
VEuPathDBi | HostDB:ENSMUSG00000071337 |
Phylogenomic databases
eggNOGi | KOG0148, Eukaryota |
GeneTreei | ENSGT00940000154962 |
InParanoidi | P52912 |
OMAi | ESFQNQH |
OrthoDBi | 1066369at2759 |
PhylomeDBi | P52912 |
TreeFami | TF312915 |
Enzyme and pathway databases
Reactomei | R-MMU-6803529, FGFR2 alternative splicing |
Miscellaneous databases
BioGRID-ORCSi | 21841, 4 hits in 74 CRISPR screens |
ChiTaRSi | Tia1, mouse |
EvolutionaryTracei | P52912 |
PROi | PR:P52912 |
RNActi | P52912, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000071337, Expressed in midbrain and 305 other tissues |
ExpressionAtlasi | P52912, baseline and differential |
Genevisiblei | P52912, MM |
Family and domain databases
CDDi | cd12615, RRM1_TIA1, 1 hit cd12618, RRM2_TIA1, 1 hit cd12621, RRM3_TIA1, 1 hit |
Gene3Di | 3.30.70.330, 3 hits |
IDEALi | IID50284 |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR003954, RRM_dom_euk IPR034827, TIA-1_RRM1 IPR034830, TIA1_RRM2 IPR034832, TIA1_RRM3 |
Pfami | View protein in Pfam PF00076, RRM_1, 3 hits |
SMARTi | View protein in SMART SM00360, RRM, 3 hits SM00361, RRM_1, 3 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | TIA1_MOUSE | |
Accessioni | P52912Primary (citable) accession number: P52912 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references