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UniProtKB - P52910 (ACS2_YEAST)
Protein
Acetyl-coenzyme A synthetase 2
Gene
ACS2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the production of acetyl-CoA. Provides the acetyl-CoA source for histone acetylation in the nucleus. 'Anaerobic' isozyme of acetyl-coenzyme A synthetase, which is required for growth on fermentable carbon sources such as glucose. May be involved in the PDH (pyruvate dehydrogenase complex) bypass.
2 PublicationsMiscellaneous
Present with 225000 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:6.2.1.11 Publication
Kineticsi
- KM=8.8 mM for acetate1 Publication
- KM=1.3 mM for ATP1 Publication
- Vmax=0.34 µmol/min/mg enzyme1 Publication
: pyruvate metabolism Pathwayi
This protein is involved in the pathway pyruvate metabolism, which is part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the pathway pyruvate metabolism and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 325 | Coenzyme ABy similarity | 1 | |
Binding sitei | 516 | AMPBy similarity | 1 | |
Binding sitei | 516 | ATPBy similarity | 1 | |
Binding sitei | 531 | AMPBy similarity | 1 | |
Binding sitei | 531 | ATPBy similarity | 1 | |
Binding sitei | 539 | Coenzyme A; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 542 | ATPBy similarity | 1 | |
Binding sitei | 612 | Coenzyme ABy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 401 – 403 | ATPBy similarity | 3 | |
Nucleotide bindingi | 425 – 430 | AMPBy similarity | 6 | |
Nucleotide bindingi | 425 – 430 | ATPBy similarity | 6 |
GO - Molecular functioni
- acetate-CoA ligase activity Source: SGD
- acid-ammonia (or amide) ligase activity Source: SGD
- AMP binding Source: InterPro
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- acetyl-CoA biosynthetic process Source: SGD
- acetyl-CoA biosynthetic process from acetate Source: InterPro
- histone acetylation Source: SGD
- pyruvate metabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.2.1.1, 984 |
Reactomei | R-SCE-71384, Ethanol oxidation |
SABIO-RKi | P52910 |
UniPathwayi | UPA00231 |
Names & Taxonomyi
Protein namesi | Recommended name: Acetyl-coenzyme A synthetase 21 Publication (EC:6.2.1.11 Publication)Alternative name(s): Acetate--CoA ligase 21 Publication Acyl-activating enzyme 21 Publication |
Gene namesi | Name:ACS21 Publication Ordered Locus Names:YLR153C ORF Names:L9634.10 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004143, ACS2 |
VEuPathDBi | FungiDB:YLR153C |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000208421 | 1 – 683 | Acetyl-coenzyme A synthetase 2Add BLAST | 683 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 506 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 679 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P52910 |
PaxDbi | P52910 |
PRIDEi | P52910 |
PTM databases
iPTMneti | P52910 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 31422, 353 interactors |
DIPi | DIP-992N |
IntActi | P52910, 45 interactors |
MINTi | P52910 |
STRINGi | 4932.YLR153C |
Miscellaneous databases
RNActi | P52910, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 206 – 209 | Coenzyme A bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Phylogenomic databases
eggNOGi | KOG1175, Eukaryota |
GeneTreei | ENSGT00940000176537 |
HOGENOMi | CLU_000022_3_6_1 |
InParanoidi | P52910 |
OMAi | AIKASWP |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR011904, Ac_CoA_lig IPR032387, ACAS_N IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF16177, ACAS_N, 1 hit PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
TIGRFAMsi | TIGR02188, Ac_CoA_lig_AcsA, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
P52910-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTIKEHKVVY EAHNVKALKA PQHFYNSQPG KGYVTDMQHY QEMYQQSINE
60 70 80 90 100
PEKFFDKMAK EYLHWDAPYT KVQSGSLNNG DVAWFLNGKL NASYNCVDRH
110 120 130 140 150
AFANPDKPAL IYEADDESDN KIITFGELLR KVSQIAGVLK SWGVKKGDTV
160 170 180 190 200
AIYLPMIPEA VIAMLAVARI GAIHSVVFAG FSAGSLKDRV VDANSKVVIT
210 220 230 240 250
CDEGKRGGKT INTKKIVDEG LNGVDLVSRI LVFQRTGTEG IPMKAGRDYW
260 270 280 290 300
WHEEAAKQRT YLPPVSCDAE DPLFLLYTSG STGSPKGVVH TTGGYLLGAA
310 320 330 340 350
LTTRYVFDIH PEDVLFTAGD VGWITGHTYA LYGPLTLGTA SIIFESTPAY
360 370 380 390 400
PDYGRYWRII QRHKATHFYV APTALRLIKR VGEAEIAKYD TSSLRVLGSV
410 420 430 440 450
GEPISPDLWE WYHEKVGNKN CVICDTMWQT ESGSHLIAPL AGAVPTKPGS
460 470 480 490 500
ATVPFFGINA CIIDPVTGVE LEGNDVEGVL AVKSPWPSMA RSVWNHHDRY
510 520 530 540 550
MDTYLKPYPG HYFTGDGAGR DHDGYYWIRG RVDDVVNVSG HRLSTSEIEA
560 570 580 590 600
SISNHENVSE AAVVGIPDEL TGQTVVAYVS LKDGYLQNNA TEGDAEHITP
610 620 630 640 650
DNLRRELILQ VRGEIGPFAS PKTIILVRDL PRTRSGKIMR RVLRKVASNE
660 670 680
AEQLGDLTTL ANPEVVPAII SAVENQFFSQ KKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S79456 Genomic DNA Translation: AAB35143.1 U53879 Genomic DNA Translation: AAB82387.1 Z73325 Genomic DNA Translation: CAA97725.1 BK006945 Genomic DNA Translation: DAA09464.1 |
PIRi | S65002 |
RefSeqi | NP_013254.1, NM_001182040.1 |
Genome annotation databases
EnsemblFungii | YLR153C_mRNA; YLR153C; YLR153C |
GeneIDi | 850846 |
KEGGi | sce:YLR153C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S79456 Genomic DNA Translation: AAB35143.1 U53879 Genomic DNA Translation: AAB82387.1 Z73325 Genomic DNA Translation: CAA97725.1 BK006945 Genomic DNA Translation: DAA09464.1 |
PIRi | S65002 |
RefSeqi | NP_013254.1, NM_001182040.1 |
3D structure databases
SMRi | P52910 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 31422, 353 interactors |
DIPi | DIP-992N |
IntActi | P52910, 45 interactors |
MINTi | P52910 |
STRINGi | 4932.YLR153C |
PTM databases
iPTMneti | P52910 |
Proteomic databases
MaxQBi | P52910 |
PaxDbi | P52910 |
PRIDEi | P52910 |
Genome annotation databases
EnsemblFungii | YLR153C_mRNA; YLR153C; YLR153C |
GeneIDi | 850846 |
KEGGi | sce:YLR153C |
Organism-specific databases
SGDi | S000004143, ACS2 |
VEuPathDBi | FungiDB:YLR153C |
Phylogenomic databases
eggNOGi | KOG1175, Eukaryota |
GeneTreei | ENSGT00940000176537 |
HOGENOMi | CLU_000022_3_6_1 |
InParanoidi | P52910 |
OMAi | AIKASWP |
Enzyme and pathway databases
UniPathwayi | UPA00231 |
BRENDAi | 6.2.1.1, 984 |
Reactomei | R-SCE-71384, Ethanol oxidation |
SABIO-RKi | P52910 |
Miscellaneous databases
PROi | PR:P52910 |
RNActi | P52910, protein |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR011904, Ac_CoA_lig IPR032387, ACAS_N IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF16177, ACAS_N, 1 hit PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
TIGRFAMsi | TIGR02188, Ac_CoA_lig_AcsA, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACS2_YEAST | |
Accessioni | P52910Primary (citable) accession number: P52910 Secondary accession number(s): D6VYE8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families